公开(公告)号：US07368554B2

公开(公告)日：2008-05-06

申请号：US11346468

申请日：2006-02-02

申请人： Glen E Mikesell ,  Han Chang ,  Robert James Peach

发明人： Glen E Mikesell ,  Han Chang ,  Robert James Peach

IPC分类号： C07H21/04 ,  C12N15/00 ,  C12N1/21 ,  C12N5/16 ,  C12N21/02

CPC分类号： C07K14/70532 ,  A61K38/00 ,  A61K38/1774 ,  A61K39/39533 ,  A61K2039/505 ,  C07K16/2827 ,  C07K19/00 ,  C07K2317/56 ,  C07K2317/73 ,  C07K2317/76 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/03 ,  C07K2319/10 ,  C07K2319/30 ,  C07K2319/40 ,  G01N33/5005 ,  Y02A50/396

摘要： The present invention provides nucleic acids encoding B7-related factors that modulate the activation of immune or inflammatory response cells, such as T-cells. Also provided are expression vectors and fusion constructs comprising nucleic acids encoding B7-related polypeptides, including BSL1, BSL2, and BSL3. The present invention further provides isolated B7-related polypeptides, isolated fusion proteins comprising B7-related polypeptides, and antibodies that are specifically reactive with B7-related polypeptides, or portions thereof. In addition, the present invention provides assays utilizing B7-related nucleic acids, polypeptides, or peptides. The present invention further provides compositions of B7-related nucleic acids, polypeptides, fusion proteins, or antibodies that are useful for the immunomodulation of a human or animal subject.

摘要翻译： 本发明提供编码调节免疫或炎性反应细胞如T细胞活化的B7相关因子的核酸。 还提供了包含编码B7相关多肽（包括BSL1，BSL2和BSL3）的核酸的表达载体和融合构建体。 本发明进一步提供分离的B7相关多肽，分离的包含B7相关多肽的融合蛋白，以及与B7相关多肽特异性反应的抗体或其部分。 此外，本发明提供利用B7相关核酸，多肽或肽的测定法。 本发明还提供可用于人或动物受试者的免疫调节的B7相关核酸，多肽，融合蛋白或抗体的组合物。

公开(公告)号：US5712117A

公开(公告)日：1998-01-27

申请号：US385500

申请日：1995-02-08

申请人： Cindy A. Sprecher

发明人： Cindy A. Sprecher

IPC分类号： C12N15/09 ,  A61K35/76 ,  A61K38/55 ,  C07K1/14 ,  C07K1/22 ,  C07K14/81 ,  C12N5/10 ,  C12P21/02 ,  C12R1/91 ,  C12N15/15 ,  C12N15/63 ,  C12N21/02

CPC分类号： C07K14/8121

摘要： Cytoplasmic Antiproteinase-2 and Cytoplasmic Antiproteinase-3 nucleic acids and serine protease inhibitor proteins encoded thereby are useful in the purification of proteins and in the treatment of inflammatory diseases and diseases involving apoptosis.

摘要翻译： 由此编码的细胞质抗蛋白酶-2和细胞质抗蛋白酶-3核酸和丝氨酸蛋白酶抑制蛋白可用于蛋白质的纯化和用于治疗炎性疾病和涉及细胞凋亡的疾病。

公开(公告)号：US07476536B2

公开(公告)日：2009-01-13

申请号：US10477471

申请日：2002-05-10

申请人： Yoshimi Kuroiwa ,  Kazuma Tomizuka ,  Hitoshi Yoshida ,  Isao Ishida

发明人： Yoshimi Kuroiwa ,  Kazuma Tomizuka ,  Hitoshi Yoshida ,  Isao Ishida

IPC分类号： C12N15/63 ,  C12N21/02 ,  C12N15/00

CPC分类号： A01K67/0278 ,  A01K67/0275 ,  A01K67/0276 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/052 ,  A01K2217/054 ,  A01K2217/15 ,  A01K2227/105 ,  A01K2267/01 ,  A01K2267/0381 ,  A61K48/00 ,  A61K2039/505 ,  C07K16/00 ,  C07K16/243 ,  C07K2317/21 ,  C12N15/8509 ,  C12N15/87 ,  C12N15/90 ,  C12N2800/30

摘要： The present invention relates to a human artificial chromosome which is genetically transmissible to the next generation with high efficiency and the method for using the same. More specifically, the present invention relates to: a human artificial chromosome in which an about 3.5 Mb to about 1 Mb region containing an antibody λ light chain gene derived from human chromosome 22 is bound to a chromosome fragment which is transmissible to a progeny through a germ line of a non-human animal, said chromosome fragment is derived from another human chromosome; a non-human animal carrying the human artificial chromosome and an offspring thereof; a method for producing the non-human animal; a method for producing a human antibody using the non-human animal or an offspring thereof; and a human antibody-producing mouse carrying the human artificial chromosome.

摘要翻译： 本发明涉及一种以高效率遗传传播到下一代的人类人造染色体及其使用方法。 更具体地，本发明涉及：人类人造染色体，其中含有源自人染色体22的抗体λ轻链基因的约3.5Mb至约1Mb区域与染色体片段结合，所述染色体片段可通过 生殖系的非人类动物，所述染色体片段衍生自另一个人染色体; 携带人类人造染色体的非人类动物及其后代; 一种生产非人动物的方法; 使用非人动物或其后代生产人抗体的方法; 和携带人类人造染色体的人抗体生产小鼠。

公开(公告)号：US07041813B2

公开(公告)日：2006-05-09

申请号：US10156275

申请日：2002-05-28

申请人： Elizabeth J. Haanes ,  Rexann S. Frank

发明人： Elizabeth J. Haanes ,  Rexann S. Frank

IPC分类号： C12N21/02 ,  C12N21/04 ,  C12P15/82 ,  C07K14/00

CPC分类号： C12N15/86 ,  C07K14/005 ,  C12N7/00 ,  C12N9/1211 ,  C12N2710/16721 ,  C12N2710/16722 ,  C12N2710/16743

摘要： The present invention includes novel recombinant canine herpes virus (CHV) and novel recombinant CHV genomes, and particularly to those CHV and CHV genomes that contain heterologous nucleic acid molecules. The present invention also relates to the use of such genomes and viruses in a variety of applications, including as therapeutic compositions to protect animals from disease. The present invention also relates to novel isolated CHV nucleic acid molecules, to CHV proteins encoded by such nucleic acid molecules, and to antibodies raised against such CHV proteins as well as to the use of such CHV nucleic acid molecules, proteins and antibodies as therapeutic compositions to protect an animal from CHV. The present invention also includes constructs comprising CHV nucleic acid molecules that include heterologous nucleic acid molecules, to recombinant vectors including such constructs, and to the use of such constructs and vectors in the production of recombinant CHV and recombinant CHV genomes.

摘要翻译： 本发明包括新型重组犬疱疹病毒（CHV）和新型重组CHV基因组，特别是含有异源核酸分子的那些CHV和CHV基因组。 本发明还涉及这些基因组和病毒在各种应用中的应用，包括作为保护动物免受疾病的治疗组合物。 本发明还涉及新的分离的CHV核酸分子，由这样的核酸分子编码的CHV蛋白，以及针对这种CHV蛋白产生的抗体，以及使用这样的CHV核酸分子，蛋白质和抗体作为治疗组合物 保护动物免受CHV的伤害。 本发明还包括包含包含异源核酸分子的CHV核酸分子，包含这种构建体的重组载体的构建体，以及这些构建体和载体在重组CHV和重组CHV基因组的产生中的用途。

公开(公告)号：US07365184B2

公开(公告)日：2008-04-29

申请号：US10823396

申请日：2004-04-12

申请人： Marcus B. Jones ,  Martin J. Blaser ,  Thomas Wood ,  Dacheng Ren

发明人： Marcus B. Jones ,  Martin J. Blaser ,  Thomas Wood ,  Dacheng Ren

IPC分类号： C07H21/04 ,  C12N15/00 ,  C12N21/02 ,  C12P21/04 ,  C12P21/06

CPC分类号： C07K14/32

摘要： The present invention pertains to the discovery that B. anthracis possesses a luxS gene that encodes a functional LuxS polypeptide, and that B. anthracis synthesizes a functional AI-2 quorum-sensing molecule. The invention provides mutant B. anthracis bacteria lacking the function of the luxS gene, which do not produce a functional AI-2 molecule and have growth defects compared to wild-type B. anthracis. The invention also concerns methods for inhibiting the growth of B. anthracis, or for preventing or treating B. anthracis infection, by inhibiting the activity of the B. anthracis LuxS polypeptide, or by exposure of the B. anthracis to furanone. In particular, the invention concerns the use of furanone, a compound that inhibits AI-2-mediated quorum-sensing, to inhibit the growth of B. anthracis, to inhibit B. anthracis toxin production, particularly that of protective antigen, and to prevent or treat B. anthracis infection. The invention also provides methods to prevent B. anthracis infection, or enhance an immune response to B. anthracis infection, by administering a vaccine comprising a B. anthracis cell in which the luxS gene is mutated.

摘要翻译： 本发明涉及炭疽芽孢杆菌具有编码功能性LuxS多肽的luxS基因的发现，并且炭疽杆菌合成功能性的AI-2群体感应分子。 本发明提供了与野生型炭疽芽孢杆菌相比，不产生功能性AI-2分子并具有生长缺陷的缺乏luxS基因功能的突变体B.炭疽杆菌。 本发明还涉及通过抑制炭疽芽孢杆菌LuxS多肽的活性或通过将炭疽杆菌暴露于呋喃酮而抑制炭疽芽孢杆菌生长或预防或治疗炭疽杆菌感染的方法。 特别地，本发明涉及使用呋喃酮（一种抑制AI-2介导的群体感应的化合物）来抑制炭疽芽孢杆菌的生长，抑制炭疽杆菌毒素生产，特别是保护性抗原的产生，并防止 或治疗炭疽杆菌感染。 本发明还提供了通过施用包含luxS基因突变的炭疽杆菌细胞的疫苗来预防炭疽杆菌感染或增强对炭疽杆菌感染的免疫应答的方法。

公开(公告)号：US06919183B2

公开(公告)日：2005-07-19

申请号：US10050279

申请日：2002-01-16

申请人： James P. Fandl ,  Neil Stahl ,  Gang Chen ,  George D. Yancopoulos

发明人： James P. Fandl ,  Neil Stahl ,  Gang Chen ,  George D. Yancopoulos

IPC分类号： A01K67/027 ,  C07K14/315 ,  C07K14/52 ,  C07K16/28 ,  C12N5/10 ,  C12N15/09 ,  C40B30/04 ,  G01N33/569 ,  G01N33/68 ,  G01N33/53 ,  C12N21/02 ,  C12N21/08

CPC分类号： C40B30/04 ,  A01K2217/05 ,  A01K2267/01 ,  C07K14/315 ,  C07K14/52 ,  C07K16/2863 ,  C07K2317/77 ,  C07K2319/00 ,  C12N15/1051 ,  G01N33/56966 ,  G01N33/6845 ,  G01N2500/10

摘要： A method for identifying and isolating cells which produce secreted proteins. This method is based upon a specific characteristic or the expression level of the secreted protein by transiently capturing the secreted protein on the surface of an individual cell, allowing selection of rare cell clones from a heterogeneous population. Also provided is the use of this method to generate cells which produce a desired level of secreted protein or secreted protein of a particular characteristic(s), and organisms which possess such cells. In particular, the method allows rapid isolation of high expression recombinant antibody-producing cell lines, or may be applied directly to rapid isolation of specific hybridomas, or to the isolation of antibody-producing transgenic animals. This method is applicable for any cell which secretes protein.

摘要翻译： 用于鉴定和分离产生分泌蛋白的细胞的方法。 该方法基于通过瞬时捕获单个细胞表面上的分泌蛋白质的分泌蛋白质的特定特征或表达水平，允许从异质群体中选择稀有细胞克隆。 还提供了这种方法用于产生产生具有特定特征的所需水平的分泌蛋白质或分泌蛋白质的细胞以及具有这种细胞的生物体的用途。 特别地，该方法允许高表达重组抗体产生细胞系的快速分离，或者可以直接应用于快速分离特异性杂交瘤，或分离产生抗体的转基因动物。 该方法适用于分泌蛋白质的任何细胞。

公开(公告)号：US5827684A

公开(公告)日：1998-10-27

申请号：US231342

申请日：1994-04-19

申请人： Kotikanyadanam Sreekrishna ,  William D. Prevatt ,  Gregory P. Thill ,  Geneva R. Davis ,  Patricia Koutz ,  Kathryn A. Barr ,  Sharon A. Hopkins

发明人： Kotikanyadanam Sreekrishna ,  William D. Prevatt ,  Gregory P. Thill ,  Geneva R. Davis ,  Patricia Koutz ,  Kathryn A. Barr ,  Sharon A. Hopkins

IPC分类号： A01N63/00 ,  A01N63/02 ,  A01N63/04 ,  C07K14/32 ,  C12N1/19 ,  C12N15/09 ,  C12N15/81 ,  C12P21/02 ,  C12R1/07 ,  C12R1/84 ,  C12N15/32 ,  C12N15/63 ,  C12N21/02

CPC分类号： C12N15/815 ,  A01N63/04 ,  C07K14/32

摘要： A method for producing one or more Bacillus toxin polypeptides by culturing methylotrophic yeast cells which have a gene(s) capable of expressing the Bacillus toxin polypeptide(s) in such cells under conditions that the gene(s) is/are transcribed is provided. The toxin polypeptide encoding segment of the gene(s) has a G+C content of about 40%-55%, and preferably comprises methylotrophic yeast codons. The preferred species of yeast for expressing such synthetic Bacillus toxin gene(s) is Pichia pastoris. Bacillus toxin polypeptides encoded by synthetic genes are expressed at high levels in transformed methylotrophic yeast cells. The toxin expressing cells may be administered as live cells or heat-killed whole cells to provide an insecticidal composition for killing susceptible insect larvae. Also provided by the present invention are DNAs capable of transforming methylotrophic yeast to express one or more Bacillus toxin polypeptides, cultures of such yeast cells transformed with such DNAs and novel Bacillus toxin polypeptides made by the method of the invention. The transformed yeast cells of the present invention are readily ingested as food by insect larvae which are susceptible to the toxin polypeptides.

摘要翻译： 提供了一种通过培养具有能够在基因被转录的条件下能够在这些细胞中表达芽孢杆菌毒素多肽的基因的甲基营养酵母细胞产生一种或多种芽孢杆菌毒素多肽的方法。 编码基因片段的毒素多肽的G + C含量为约40％-55％，优选包含甲基营养酵母密码子。 用于表达这种合成芽孢杆菌毒素基因的酵母的优选种类是巴斯德毕赤酵母。 由合成基因编码的芽孢杆菌毒素多肽在转化的甲基营养酵母细胞中以高水平表达。 表达毒素的细胞可以作为活细胞或热灭活的全细胞施用，以提供用于杀灭易感昆虫幼虫的杀虫组合物。 本发明还提供能够转化甲基营养酵母以表达一种或多种芽孢杆菌毒素多肽的DNA，用这种DNA转化的这种酵母细胞的培养物和通过本发明的方法制备的新的芽孢杆菌毒素多肽。 本发明的转化酵母细胞易于被毒素多肽易感的昆虫幼虫摄取为食物。

公开(公告)号：US07604968B2

公开(公告)日：2009-10-20

申请号：US11069633

申请日：2005-03-01

申请人： Claudia Schmidt-Dannert ,  Kevin Watts

发明人： Claudia Schmidt-Dannert ,  Kevin Watts

IPC分类号： C12P9/00 ,  C12N9/10 ,  C12N9/88 ,  C12N21/02 ,  A61K31/047 ,  A61K31/05 ,  C07C39/10

CPC分类号： C12N9/1029 ,  C12N9/0042 ,  C12N9/0073 ,  C12N9/1037 ,  C12N9/88 ,  C12N9/93 ,  C12P7/22 ,  C12P7/26 ,  C12P17/06

摘要： The invention provides methods and materials related to producing flavonoids as well as other organic compounds. For example, the invention provides isolated nucleic acids, polypeptides, host cells, and methods and materials for producing flavonoids and other organic compounds.

摘要翻译： 本发明提供了与生产类黄酮以及其它有机化合物相关的方法和材料。 例如，本发明提供分离的核酸，多肽，宿主细胞，以及用于产生类黄酮和其它有机化合物的方法和材料。

公开(公告)号：US5817499A

公开(公告)日：1998-10-06

申请号：US578590

申请日：1996-01-03

申请人： Henrik Dalb.o slashed.ge ,  Stephan Christgau ,  Lene Nonboe Andersen ,  Lene Venke Kofod ,  Markus Sakari Kauppinen

发明人： Henrik Dalb.o slashed.ge ,  Stephan Christgau ,  Lene Nonboe Andersen ,  Lene Venke Kofod ,  Markus Sakari Kauppinen

IPC分类号： C12N9/42 ,  C12N15/56 ,  C12N5/10 ,  C12N9/24 ,  C12N21/02

CPC分类号： C12N9/2437 ,  C12Y302/01004

摘要： DNA encoding an endoglucanase from Trichoderma harzianum is disclosed. The endoglucanase has activity toward mixed .beta.-1,3-1,4 glucans and is especially useful in brewing processes.

摘要翻译： PCT No.PCT / DK94 / 00275 Sec。 371日期1996年1月3日 102（e）日期1996年1月3日PCT 1994年7月5日PCT公布。 公开号WO95 / 02043 1995年1月19日公开了编码哈茨木霉的内切葡聚糖酶的DNA。 内切葡聚糖酶具有对混合的β-1,3-1,4葡聚糖的活性，并且在酿造过程中特别有用。

公开(公告)号：US5252476A

公开(公告)日：1993-10-12

申请号：US222352

申请日：1988-07-20

申请人： Robert A. Hallewell ,  Guy T. Mullenbach

发明人： Robert A. Hallewell ,  Guy T. Mullenbach

IPC分类号： C12N9/02 ,  C12N15/53 ,  C12N15/62 ,  C12N15/70 ,  C12N15/81 ,  C12N21/02 ,  C12N1/19

CPC分类号： C12N9/0089 ,  C12N15/62 ,  C12N15/70 ,  C12N15/81 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/735 ,  C07K2319/90 ,  Y10S435/942

摘要： Methods and compositions are provided for the production of human superoxide dismutase and a novel protocol for enhancing efficiency of expression. The gene encoding for human superoxide dismutase is isolated and inserted into a vector in conjunction with a synthetic linker which provides for enhanced efficiency in translation.E. coli strain D1210 (pSOD.times.8) was deposited at the A.T.C.C. on Sep. 27, 1983 and given Accession No. 39453. Yeast strain 2150-2-3 (pC1/1GAPSOD) and E. coli strains D1210 (pSOD11) and D1210 (pS2OR) were deposited at the A.T.C.C. on May 9, 1984, and given Accession Nos. 20708, 39679 and 39,680, respectively.

摘要翻译： 提供了用于生产人超氧化物歧化酶的方法和组合物，以及用于增强表达效率的新方案。 分离编码人超氧化物歧化酶的基因，并与合成连接子一起插入载体，其提供翻译效率的提高。 大肠杆菌菌株D1210（pSODx8）保藏在A.T.C.C. 1983年9月27日，授予登录号39453.酵母菌株2150-2-3（pC1 / 1GAPSOD）和大肠杆菌菌株D1210（pSOD11）和D1210（pS2OR）沉积在A.T.C.C. 于一九八四年五月九日，分别获得加拿大号20708,399679及39,680。