公开(公告)号：US20160039915A1

公开(公告)日：2016-02-11

申请号：US14688449

申请日：2015-04-16

申请人： Boston Biomedical Research Institute

发明人： Victor Raso

IPC分类号： C07K16/18

CPC分类号： C07K16/18 ,  A61K38/00 ,  A61K39/0007 ,  A61K39/3955 ,  C07K2317/34 ,  C12N9/0002 ,  Y10S977/729 ,  Y10S977/896 ,  Y10S977/915 ,  Y10S977/918

摘要： Disclosed herein are compositions and methods useful for controlling β-amyloid levels. In particular, the instant invention relates to an antibody that catalyzes hydrolysis of β-amyloid at a predetermined amide linkage are provided. The present invention also provides a vectorized antibody that is capable of crossing the blood brain barrier and is also capable of catalyzing the hydrolysis of β-amyloid at a predetermined amide linkage. Also provided are methods for modulating β-amyloid levels in vivo using antibodies that bind to β-amyloid. These compositions and methods have therapeutic applications, including the treatment of Alzheimer's disease.

摘要翻译： 本文公开了可用于控制和淀粉样蛋白水平的组合物和方法。 特别地，本发明涉及以预定的酰胺键催化水解淀粉样蛋白的抗体。 本发明还提供能够穿过血脑屏障的载体化抗体，并且还能够以预定的酰胺键催化β-淀粉样蛋白的水解。 还提供了使用与β-淀粉样蛋白结合的抗体在体内调节和淀粉样蛋白水平的方法。 这些组合物和方法具有治疗应用，包括治疗阿尔茨海默氏病。

公开(公告)号：US20140234948A1

公开(公告)日：2014-08-21

申请号：US14260099

申请日：2014-04-23

申请人： The USA, as represented by the Secretary, Dept of Health and Human Services

发明人： Thomas D. Schneider ,  Ilya G. Lyakhov

IPC分类号： C12M1/00

CPC分类号： C12M99/00 ,  B82B1/00 ,  B82B3/00 ,  B82Y30/00 ,  B82Y40/00 ,  C07K14/4716 ,  H02N11/006 ,  Y10S977/729 ,  Y10T29/4984 ,  Y10T156/10

摘要： A molecular motor in which multiple concentric cylinders (or nested cones) rotate around a common longitudinal axis. Opposing complementary surfaces of the cylinders or cones are coated with complementary motor protein pairs (such as actin and myosin). The actin and myosin interact with one another in the presence of ATP to rotate the cylinders or cones relative to one another, and this rotational energy is harnessed to produce work. The concentration of ATP and the number of nested cylinders or cones can be used to control the rotational speed of the motor. The length of the cylinders can also be used to control the power generated by the motor. In another embodiment, the molecular motor includes at least two annular substrates wherein one annular substrate is coated with a first motor protein and the other annular substrate is coated with a second motor protein. The first and second motor proteins interact with each other to move the second annular relative to the first annular substrate.

摘要翻译： 一个分子马达，其中多个同心圆柱体（或嵌套圆锥体）围绕共同的纵向轴线旋转。 圆柱体或锥体的互补表面涂覆有互补的运动蛋白质对（如肌动蛋白和肌球蛋白）。 肌动蛋白和肌球蛋白在ATP存在下相互作用以相对于彼此旋转圆柱体或锥体，并且利用该旋转能量来产生作用。 ATP的浓度和嵌套圆柱体或锥体的数量可用于控制电机的转速。 气缸的长度也可用于控制电机产生的电力。 在另一个实施例中，分子马达包括至少两个环形基底，其中一个环形基底涂覆有第一运动蛋白，另一个环形基底涂覆有第二运动蛋白。 第一和第二运动蛋白相互作用以相对于第一环形基底移动第二环状物。

公开(公告)号：US20110237012A1

公开(公告)日：2011-09-29

申请号：US12766280

申请日：2010-04-23

申请人： Jyong Sik JANG ,  Oh Seok Kwon ,  Seon Joo Park

发明人： Jyong Sik JANG ,  Oh Seok Kwon ,  Seon Joo Park

IPC分类号： H01L21/04

CPC分类号： G01N33/5438 ,  G01N27/4145 ,  G01N27/4146 ,  Y10S977/716 ,  Y10S977/729 ,  Y10S977/742

摘要： Disclosed is a method for fabricating a high-performance field-effect transistor biosensor for diagnosing cancers using micro conductive polymer nanomaterials funtionalized with anti-VEGF aptamer. Disclosed is a high-sensitivity field-effect transistor biosensor for diagnosing cancers using a micro conductive polymer nanomaterial transistor array including a micro polymer nanomaterial transistor array including a channel region provided with a metal source electrode, a metal drain electrode, a gate and micro polymer nanomaterials, and an anti-VEGF aptamer covalently bound to the surface of the micro polymer nanomaterials constituting the channel region of the micro polymer nanomaterials transistor array, to target VEGF (Vascular endothelial growth factor).

摘要翻译： 本发明公开了一种使用由抗VEGF适体功能化的微导电聚合物纳米材料制造用于诊断癌症的高性能场效应晶体管生物传感器的方法。 公开了一种高灵敏度场效应晶体管生物传感器，用于使用包括微聚合物纳米材料晶体管阵列的微导电聚合物纳米材料晶体管阵列诊断癌症，所述微聚合物纳米材料晶体管阵列包括设置有金属源电极，金属漏电极，栅极和微聚合物的沟道区 纳米材料和共价结合到构成微聚合物纳米材料晶体管阵列的沟道区的微聚合物纳米材料的表面的抗VEGF适体靶向VEGF（血管内皮生长因子）。

公开(公告)号：US07449445B2

公开(公告)日：2008-11-11

申请号：US11252719

申请日：2005-10-19

申请人： Kentaro Onizuka ,  Shuhei Tanaka ,  Hirokazu Sugihara ,  Atsuo Tamura

发明人： Kentaro Onizuka ,  Shuhei Tanaka ,  Hirokazu Sugihara ,  Atsuo Tamura

IPC分类号： A61K38/00 ,  A61K51/00 ,  A61K38/04 ,  C07K2/00 ,  C07K4/00 ,  C07K5/00 ,  C07K7/00 ,  C07K14/00 ,  C07K16/00 ,  C07K17/00 ,  A61M36/14 ,  A01N37/18

CPC分类号： C07K7/06 ,  C07K14/001 ,  Y10S930/28 ,  Y10S977/705 ,  Y10S977/729

摘要： A peptide nanofiber having conductivity is provided. A conductive peptide nanofiber which includes a nanofiber formed through a manner of self-assembly of a peptide that has a nanofiber-forming ability and consists of an amino acid sequence of Xaa-Phe-Ile-Val-Ile-Phe-Xaa (SEQ ID NO: 1, wherein N-terminal Xaa is an arbitrary amino acid residue Xaa1; C-terminal Xaa is an arbitrary amino acid residue Xaa2; and Xaa1 and Xaa2 are an amino acid having an acidic side chain, an amino acid having a basic side chain, or an amino acid having a side chain with polarity according as acidity and basicity) or a derivative of the peptide and a conductive substance added thereto, the aforementioned conductive substance being added to an amino group of the peptide or the derivative.

摘要翻译： 提供了具有导电性的肽纳米纤维。 一种导电肽纳米纤维，其包括通过具有纳米纤维形成能力并由Xaa-Phe-Ile-Val-Ile-Phe-Xaa的氨基酸序列组成的肽的自组装形式的纳米纤维（SEQ ID NO： NO：1，其中N-末端Xaa是任意的氨基酸残基Xaa 1 C末端Xaa是任意的氨基酸残基Xaa 2和Xaa < 1和Xaa 2是具有酸性侧链，具有碱性侧链的氨基酸或具有根据酸度和碱度的极性侧链的氨基酸的氨基酸） 或肽的衍生物和加入其中的导电物质，将上述导电物质加入肽或其衍生物的氨基。

公开(公告)号：US20020136718A1

公开(公告)日：2002-09-26

申请号：US09992994

申请日：2001-11-06

申请人： Boston Biomedical Research Institute

发明人： Victor Raso

IPC分类号： A61K039/395 ,  G01N033/53 ,  G01N033/537 ,  G01N033/543 ,  C12N009/00

CPC分类号： C07K16/18 ,  A61K38/00 ,  A61K39/0007 ,  A61K39/3955 ,  C07K2317/34 ,  C12N9/0002 ,  Y10S977/729 ,  Y10S977/896 ,  Y10S977/915 ,  Y10S977/918

摘要： The present invention provides an antibody which catalyzes hydrolysis of null-amyloid at a predetermined amide linkage. The antibody preferentially binds a transition state analog which mimics the transition state adopted by null-amyloid during hydrolysis at a predetermined amide linkage and also binds to natural null-amyloid with sufficient affinity to detect by ELISA. Alternatively, the antibody preferentially binds a transition state analog which mimics the transition state adopted by null-amyloid during hydrolysis at a predetermined amide linkage, and does not bind natural null-amyloid with sufficient affinity to detect by ELISA. Antibodies generated are characterized by the amide linkage which they hydrolyze. Specific antibodies provided include those which catalyze the hydrolysis at the amyloid linkages between residues 39 and 40, 40 and 41, and 41 and 42, of null-amyloid. The present invention also provides a vectorized antibody which is characterized by the ability to cross the blood brain barrier and is also characterized by the ability to catalyze the hydrolysis of null-amyloid at a predetermined amide linkage. The vectorized antibody can take the form of a bispecific antibody, which has a first specificity for the transferrin receptor and a second specificity for a transition state adopted by null-amyloid during hydrolysis. The present invention also provides a method for sequestering free null-amyloid in the bloodstream of an animal by intravenously administering antibodies specific for null-amyloid to the animal in an amount sufficient to increase retention of null-amyloid in the circulation. In addition, the present invention provides a method for sequestering free null-amyloid in the bloodstream of an animal by immunizing an animal with an antigen comprised of an epitope which is present on null-amyloid endogenous to the animal under conditions appropriate for the generation of antibodies which bind endogenous null-amyloid. Therapeutic applications of these methods include treating patients diagnosed with, or at risk for Alzheimer's disease. Methods for reducing levels of null-amyloid in the brain of an animal, by intravenously administering antibodies specific for endogenous null-amyloid to the animal, or by immunizing the animal with an antigen comprised of an epitope which is present on endogenous null-amyloid are also provided. In one embodiment, the antigen used to generate the antibodies is a transition state analog which mimics the transition state adopted by null-amyloid during hydrolysis at a predetermined amide linkage. Similar methods which utilize or generate antibodies which catalyze the hydrolysis of null-amyloid for reducing levels of circulating null-amyloid in an animal, and also for preventing the formation of amyloid plaques in the brain of an animal, and also for disaggregating amyloid plaques present in the brain of an animal, are also provided. Also provided is a method for generating antibodies which catalyze hydrolysis of a protein or polypeptide by immunizing an animal with an antigen comprised of an epitope which has a statine analog which mimics the conformation of a predetermined hydrolysis transition state of the polypeptide. A similar method, which utilizes reduced peptide bond analogs to mimic the conformation of a hydrolysis transition state of a polypeptide, is also provided.

公开(公告)号：US08138005B2

公开(公告)日：2012-03-20

申请号：US12766280

申请日：2010-04-23

申请人： Jyong Sik Jang ,  Oh Seok Kwon ,  Seon Joo Park

发明人： Jyong Sik Jang ,  Oh Seok Kwon ,  Seon Joo Park

IPC分类号： H01L21/00

CPC分类号： G01N33/5438 ,  G01N27/4145 ,  G01N27/4146 ,  Y10S977/716 ,  Y10S977/729 ,  Y10S977/742

摘要： Disclosed is a method for fabricating a high-performance field-effect transistor biosensor for diagnosing cancers using micro conductive polymer nanomaterials funtionalized with anti-VEGF aptamer. Disclosed is a high-sensitivity field-effect transistor biosensor for diagnosing cancers using a micro conductive polymer nanomaterial transistor array including a micro polymer nanomaterial transistor array including a channel region provided with a metal source electrode, a metal drain electrode, a gate and micro polymer nanomaterials, and an anti-VEGF aptamer covalently bound to the surface of the micro polymer nanomaterials constituting the channel region of the micro polymer nanomaterials transistor array, to target VEGF (Vascular endothelial growth factor).

摘要翻译： 本发明公开了一种使用由抗VEGF适体功能化的微导电聚合物纳米材料制造用于诊断癌症的高性能场效应晶体管生物传感器的方法。 公开了一种高灵敏度场效应晶体管生物传感器，用于使用包括微聚合物纳米材料晶体管阵列的微导电聚合物纳米材料晶体管阵列诊断癌症，所述微聚合物纳米材料晶体管阵列包括设置有金属源电极，金属漏电极，栅极和微聚合物的沟道区 纳米材料和共价结合到构成微聚合物纳米材料晶体管阵列的沟道区的微聚合物纳米材料的表面的抗VEGF适体靶向VEGF（血管内皮生长因子）。

公开(公告)号：US20040126820A1

公开(公告)日：2004-07-01

申请号：US10667004

申请日：2003-09-19

发明人： Selena Chan ,  Xing Su ,  Mineo Yamakawa

IPC分类号： G01N033/573

CPC分类号： C12Q1/6816 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y30/00 ,  Y10S977/702 ,  Y10S977/704 ,  Y10S977/705 ,  Y10S977/728 ,  Y10S977/729 ,  Y10S977/734 ,  Y10S977/742 ,  Y10S977/773 ,  Y10S977/774 ,  Y10S977/924 ,  C12Q2565/601 ,  C12Q2563/185

摘要： The methods, apparatus and compositions disclosed herein concern the detection, identification and/or sequencing of biomolecules, such as nucleic acids or proteins. In certain embodiments of the invention, coded probes comprising a probe molecule attached to one or more nano-barcodes may be allowed to bind to one or more target molecules. After binding and separation from unbound coded probes, the bound coded probes may be aligned on a surface and analyzed by scanning probe microscopy. The nano-barcodes may be any molecule or complex that is distinguishable by SPM, such as carbon nanotubes, fullerenes, submicrometer metallic barcodes, nanoparticles or quantum dots. Where the probes are oligonucleotides, adjacent coded probes hybridized to a target nucleic acid may be ligated together before alignment and SPM analysis. Compositions comprising coded probes are also disclosed herein. Systems for biomolecule analysis may comprise an SPM instrument and at least one coded probe attached to a surface.

摘要翻译： 本文公开的方法，装置和组合物涉及生物分子如核酸或蛋白质的检测，鉴定和/或测序。 在本发明的某些实施方案中，包含与一个或多个纳米条形码连接的探针分子的编码探针可以被允许与一个或多个靶分子结合。 在结合和分离未结合的编码探针之后，结合编码的探针可以在表面上对准并通过扫描探针显微镜进行分析。 纳米条形码可以是通过SPM可区分的任何分子或复合物，例如碳纳米管，富勒烯，亚微米金属条形码，纳米粒子或量子点。 当探针是寡核苷酸时，与靶核酸杂交的相邻编码探针可以在对准和SPM分析之前连接在一起。 包含编码探针的组合物也在本文中公开。 用于生物分子分析的系统可以包括SPM仪器和连接到表面的至少一个编码探针。

公开(公告)号：US20040058328A1

公开(公告)日：2004-03-25

申请号：US10251152

申请日：2002-09-20

发明人： Selena Chan ,  Xing Su ,  Mineo Yamakawa

IPC分类号： C12Q001/68 ,  G06F019/00 ,  G01N033/48 ,  G01N033/50

CPC分类号： C12Q1/6816 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y30/00 ,  Y10S977/702 ,  Y10S977/704 ,  Y10S977/705 ,  Y10S977/728 ,  Y10S977/729 ,  Y10S977/734 ,  Y10S977/742 ,  Y10S977/773 ,  Y10S977/774 ,  Y10S977/924 ,  C12Q2565/601 ,  C12Q2563/185

摘要： The methods, apparatus and compositions disclosed herein concern the detection, identification and/or sequencing of biomolecules, such as nucleic acids or proteins. In certain embodiments of the invention, coded probes comprising a probe molecule attached to one or more nanobarcodes may be allowed to bind to one or more target molecules. After binding and separation from unbound coded probes, the bound coded probes may be aligned on a surface and analyzed by scanning probe microscopy. The nanobarcodes may be any molecule or complex that is distinguishable by SPM, such as carbon nanotubes, fullerenes, submicrometer metallic barcodes, nanoparticles or quantum dots. Where the probes are oligonucleotides, adjacent coded probes hybridized to a target nucleic acid may be ligated together before alignment and SPM analysis. Compositions comprising coded probes are also disclosed herein. Systems for biomolecule analysis may comprise an SPM instrument and at least one coded probe attached to a surface.

摘要翻译： 本文公开的方法，装置和组合物涉及生物分子如核酸或蛋白质的检测，鉴定和/或测序。 在本发明的某些实施方案中，包含连接至一个或多个纳米糖基的探针分子的编码探针可以被允许与一个或多个靶分子结合。 在结合和分离未结合的编码探针之后，结合编码的探针可以在表面上对准并通过扫描探针显微镜进行分析。 纳米线可以是通过SPM可区分的任何分子或复合物，例如碳纳米管，富勒烯，亚微米金属条形码，纳米颗粒或量子点。 当探针是寡核苷酸时，与靶核酸杂交的相邻编码探针可以在对准和SPM分析之前连接在一起。 包含编码探针的组合物也在本文中公开。 用于生物分子分析的系统可以包括SPM仪器和附接到表面的至少一个编码探针。

公开(公告)号：US06656712B1

公开(公告)日：2003-12-02

申请号：US09673668

申请日：2000-12-01

申请人： Fabrice Balavoine ,  Charles Miokowski ,  Patrick Schultz ,  Cyrille Richard

发明人： Fabrice Balavoine ,  Charles Miokowski ,  Patrick Schultz ,  Cyrille Richard

IPC分类号： C12N1114

CPC分类号： C07D495/04 ,  B82Y30/00 ,  C07C233/40 ,  C07C235/20 ,  C07C2603/24 ,  C07C2603/54 ,  C07F1/005 ,  C07F15/045 ,  C30B7/00 ,  C30B29/58 ,  G01N33/54353 ,  G01N33/54373 ,  G01N33/551 ,  Y10S977/729 ,  Y10S977/742 ,  Y10S977/746 ,  Y10S977/747 ,  Y10S977/793 ,  Y10S977/842 ,  Y10S977/848 ,  Y10S977/894 ,  Y10S977/896 ,  Y10S977/898 ,  Y10S977/92

摘要： Method for the attachment and/or crystallization of macromolecules, chemical reagents used in the said method, products obtained as well as applications of the said products in the field of materials and of structural biology, in particular as biosensors or as biomaterials. The said method comprises essentially the incubation, without stirring, for at least 15 minutes, of a biological macromolecule in solution with nanotubes of carbon closed at their ends, under suitable temperature and pH conditions.

摘要翻译： 用于大分子的附着和/或结晶的方法，所述方法中使用的化学试剂，获得的产物以及所述产品在材料和结构生物学领域的应用，特别是作为生物传感器或生物材料。所述方法 在适当的温度和pH条件下基本上包括在其末端封闭碳纳米管的情况下，不搅拌至少15分钟孵育溶液中的生物大分子。

公开(公告)号：US06582945B1

公开(公告)日：2003-06-24

申请号：US09594366

申请日：2000-06-15

申请人： Victor Raso

发明人： Victor Raso

IPC分类号： C12N900

CPC分类号： C07K16/18 ,  A61K38/00 ,  A61K39/0007 ,  A61K39/3955 ,  C07K2317/34 ,  C12N9/0002 ,  Y10S977/729 ,  Y10S977/896 ,  Y10S977/915 ,  Y10S977/918

摘要： Disclosed are antibodies which catalyze hydrolysis of &bgr;-amyloid. Antibodies generated are characterized by the amide linkage which they hydrolyze. Methods of generating the antibodies by using &bgr;-amyloid peptides which incorporate transition state analogs are also provided. Also disclosed is a vectorized antibody which is characterized by the ability to cross the blood brain barrier, and is further characterized by the ability to catalyze the hydrolysis of &bgr;-amyloid. The vectorized antibody can take the form of a bispecific antibody, which has a first specificity for the transferrin receptor and a second specificity for a transition state adopted by &bgr;-amyloid during hydrolysis.

摘要翻译： 公开了催化β-淀粉样蛋白水解的抗体。 产生的抗体的特征在于它们水解的酰胺键。 还提供了通过使用结合过渡态类似物的β-淀粉样肽产生抗体的方法。 还公开了一种载体化抗体，其特征在于穿过血脑屏障的能力，其特征还在于催化β-淀粉样蛋白的水解的能力。 载体化抗体可以采用双特异性抗体的形式，其具有转铁蛋白受体的第一特异性，并且在水解期间由β-淀粉样蛋白所采用的过渡态的第​​二特异性。