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    Diagnostics and vaccines for mycobacterial infections of animals and humans
    1.
    发明授权
    Diagnostics and vaccines for mycobacterial infections of animals and humans 有权
    用于动物和人类分枝杆菌感染的诊断和疫苗

    公开(公告)号：US08148136B1

    公开(公告)日：2012-04-03

    申请号：US09646568

    申请日：1999-03-18

    申请人： John Hermon-Taylor ,  Timothy John Bull

    发明人： John Hermon-Taylor ,  Timothy John Bull

    IPC分类号： C07H21/04 ,  C07H21/02 ,  C12N1/12 ,  C12N1/20 ,  C12N15/00 ,  A01N63/00 ,  A01N65/00 ,  A61K48/00

    CPC分类号： C12N9/18 ,  A61K39/00 ,  A61K48/00 ,  Y10S435/863 ,  Y10S435/864

    摘要： This invention relates to the protein, Mycobacterium paratuberculosis acylase (mpa) and the gene encoding mpa, which we have identified in the pathogen Mycobacterium paratuberculosis Mptb (also designated Mycobacterium avium subspecies paratuberculosis MAP), and to their use in the diagnosis of Mptb/MAP infections in animals and humans, as well as their use as components of vaccines for the prevention and treatment of diseases caused by Mptb/MAP. The importance of an intact uninterrupted mpa gene as a determinant of pathogenicity in Mptb/MAP is recognized and the invention also provides attenuated strains of normally pathogenic Mptb/MAP and other mycobacteria in which mpa has been inactivated, for use as vaccines.

    摘要翻译： 本发明涉及蛋白质，副结核分枝杆菌酰基转移酶（mpa）和编码mpa的基因，我们在病原体分枝杆菌（Mycobacterium paratuberculosis）Mptb（也称为鸟分枝杆菌亚种paratuberculosis MAP）中鉴定，并且用于诊断Mptb / MAP 动物和人类的感染，以及它们作为预防和治疗由Mptb / MAP引起的疾病的疫苗的组成部分的用途。 认识到完整的不间断mpa基因作为Mptb / MAP中致病性决定簇的重要性，本发明还提供通常致病的Mptb / MAP和其它mpa已被灭活的分解细菌的减毒菌株，用作疫苗。

    Insertional mutations in mycobacteria
    2.
    发明授权
    Insertional mutations in mycobacteria 失效
    分枝杆菌中的插入突变

    公开(公告)号：US06566121B1

    公开(公告)日：2003-05-20

    申请号：US08850977

    申请日：1997-05-05

    申请人： William R. Jacobs, Jr. ,  Barry Bloom ,  Ganjam V. Kalpana ,  Jeffrey D. Cirillo ,  Ruth McAdam

    发明人： William R. Jacobs, Jr. ,  Barry Bloom ,  Ganjam V. Kalpana ,  Jeffrey D. Cirillo ,  Ruth McAdam

    IPC分类号： C12N112

    CPC分类号： A61K39/04 ,  A61K39/00 ,  C07K14/35 ,  C12N15/74 ,  Y10S435/863 ,  Y10S435/864 ,  Y10S435/865 ,  Y10S435/866

    摘要： A mutated mycobacterium selected from the class consisting of mutated M.bovis-BCG, mutated M.tuberculosis, and mutated M. leprae. The mutation of M.bovis-BCG, M.tuberculosis, or M. leprae is preferably effected through an insertional mutation of a mycobacterial gene. The insertional mutagenesis may be effected, for example, through illegitimate recombination or by a mycobacterial transposon. Such mutated mycobacteria may then be transformed with an expression vector(s) containing a complement gene to the gene which is mutated, and preferably also including a heterologous gene.

    摘要翻译： 选自突变的M.bovis-BCG，突变的结核分枝杆菌和突变的麻疯病的类别的突变的分枝杆菌。 M.bovis-BCG，结核分枝杆菌或麻疯病毒的突变优选通过分枝杆菌基因的插入突变来实现。 插入诱变可以例如通过非法重组或通过分枝杆菌转座子进行。 然后可以将这种突变的分枝杆菌用含有突变基因的补体基因的表达载体转化，并且优选还包括异源基因。

    A60-antigen from Mycobacteria and use thereof as tuberculin and as vaccine
    5.
    发明授权
    A60-antigen from Mycobacteria and use thereof as tuberculin and as vaccine 失效
    来自分枝杆菌的A60-抗原，其用作结核菌素和疫苗

    公开(公告)号：US4965192A

    公开(公告)日：1990-10-23

    申请号：US187919

    申请日：1988-04-29

    申请人： Roland F. Maes

    发明人： Roland F. Maes

    IPC分类号： G01N33/531 ,  G01N33/569

    CPC分类号： C12R1/32 ,  G01N33/531 ,  G01N33/5695 ,  Y10S435/803 ,  Y10S435/82 ,  Y10S435/863 ,  Y10S435/864 ,  Y10S435/866 ,  Y10S436/811 ,  Y10S436/815 ,  Y10S530/806 ,  Y10S530/82 ,  Y10S530/825

    摘要： An interspecific antigen of Mycobacteria consists essentially of a mixture in substantially immunochemically pure form of a protein having a molecular weight of at least about 4.times.10.sup.6 Daltons and polysaccharide having a molecular weight of at least about 1.times.10.sup.6 Daltons, and has when subjected to cross-electrophoresis an immunoelectrophoretic precipitation pattern corresponding to A60-antigen of Mycobacteria bovis strain BCG. This antigen is effective for detecting the prior exposure of a subject to Mycobacterial infections by a cutaneous test.

    摘要翻译： 分枝杆菌的种间抗原基本上由基本上免疫化学纯的形式的分子量为至少约4×10 6道尔顿的蛋白质和分子量为至少约1×10 6道尔顿的多糖组成的混合物，并且当经过交联电泳 免疫电泳沉淀模式对应于牛分枝杆菌菌株BCG的A60-抗原。 该抗原通过皮肤试验检测受试者对分枝杆菌感染的先前暴露是有效的。

    Mycobacterium fol a gene that encodes for the enzyme dihydrofolate reductase
    7.
    发明授权
    Mycobacterium fol a gene that encodes for the enzyme dihydrofolate reductase 失效
    分枝杆菌是编码二氢叶酸还原酶的基因

    公开(公告)号：US06229001B1

    公开(公告)日：2001-05-08

    申请号：US08990791

    申请日：1997-12-15

    申请人： William W. Barrow ,  Sabrina Z. Van Ginkel ,  Thomas P. Dooley ,  William J. Suling

    发明人： William W. Barrow ,  Sabrina Z. Van Ginkel ,  Thomas P. Dooley ,  William J. Suling

    IPC分类号： C07H2102

    CPC分类号： C12N9/003 ,  C07K14/35 ,  C12Q1/04 ,  C12Q1/689 ,  Y10S435/863 ,  Y10S435/864 ,  Y10S435/865 ,  Y10S435/866

    摘要： The invention relates to the nucleic acid sequence and amino acid sequence of dihydrofolate reductase (DHFR) from mycobacteria and to expression of recombinant DHFR protein. Utilizing the recombinant protein, novel therapies and diagnostic strategies can be developed and selective antimycobacterial compositions can be designed and utilized to treat mycobacterial infections in patients. This invention includes all or portions of novel recombinant nucleic acids encoding DHFR for mycobacteria such as M. avium, to novel recombinant DHFR peptides produced by such sequences, and to vaccines, diagnostic kits, cells and therapies utilizing these peptides and nucleic acid sequences. The present invention relates to methods for using the sequences of the present invention to develop drugs specific to M. avium and other mycobacteria, to identify and sequence corresponding sequences in species other than M. avium, as well as diagnostic and treatment methods incorporating the disclosed sequences and peptides.

    摘要翻译： 本发明涉及来自分枝杆菌的二氢叶酸还原酶（DHFR）和重组DHFR蛋白的表达的核酸序列和氨基酸序列。 利用重组蛋白，可以开发出新的疗法和诊断策略，并且可以设计和利用选择性抗分支杆菌组合物来治疗患者的分枝杆菌感染。 本发明包括编码用于分枝杆菌如鸟分枝杆菌的DHFR的新型重组核酸的全部或部分，以及由这些序列产生的新型重组DHFR肽以及利用这些肽和核酸序列的疫苗，诊断试剂盒，细胞和疗法。 本发明涉及使用本发明的序列开发针对鸟分枝杆菌和其他分枝杆菌特异性的药物的方法，以鉴定和序列除了鸟分枝杆菌以外的物种中的相应序列，以及结合所公开的诊断和治疗方法 序列和肽。

    Method of visually demonstrating the presence of microorganisms, identifying them, and testing them for sensitivity to antibiotics with redox indicators
    9.
    发明授权
    Method of visually demonstrating the presence of microorganisms, identifying them, and testing them for sensitivity to antibiotics with redox indicators 失效
    在视觉上显示微生物存在的方法，识别它们，并用氧化还原指示剂测试它们对抗生素的敏感性

    公开(公告)号：US5523214A

    公开(公告)日：1996-06-04

    申请号：US239808

    申请日：1994-05-09

    申请人： Jurgen Horn

    发明人： Jurgen Horn

    IPC分类号： C12Q1/04 ,  C12Q1/18 ,  C12Q1/02

    CPC分类号： C12Q1/04 ,  C12Q2304/22 ,  G01N2333/35 ,  Y10S435/863 ,  Y10S435/864 ,  Y10S435/865 ,  Y10S435/866

    摘要： A method of visually demonstrating the growth of such microorganisms as fungi, yeasts, and bacteria, especially mycobacteria, identifying them, and testing them for sensitivity to antibiotics by the color change of a specific redox indicator system. A mixture of the redox indicators Methylene Blue and resazurin is added to the culture medium. Iron(III) salts mixed with K.sub.3 Fe(CN).sub.6, Iron(II) salts mixed with K.sub.4 Fe(CN).sub.6, or sodium tungstate (Na.sub.2 WO.sub.4) is added to the culture medium.

    摘要翻译： 视觉上显示真菌，酵母菌和细菌，特别是分枝杆菌等微生物生长的鉴定方法，并通过特定的氧化还原指示剂系统的颜色变化测试它们对抗生素的敏感性。 向培养基中加入氧化还原指示剂亚甲基蓝和天蓝蛋白的混合物。 将与K 3 Fe（CN）6混合的铁（III）盐，与K4Fe（CN）6混合的铁（II）盐或钨酸钠（Na 2 WO 4））加入到培养基中。

    Apparatus for determining the presence or absence of MAI (Mycobacterium avium-intacellulars)
    10.
    发明授权
    Apparatus for determining the presence or absence of MAI (Mycobacterium avium-intacellulars) 失效
    用于确定MAI（鸟分枝杆菌 - 细胞内）的存在或不存在的装置

    公开(公告)号：US5472877A

    公开(公告)日：1995-12-05

    申请号：US340983

    申请日：1994-11-17

    申请人： Robert A. Ollar

    发明人： Robert A. Ollar

    IPC分类号： C12Q1/04 ,  C12Q1/18 ,  G01N33/569 ,  C12M1/00 ,  C12M1/14 ,  C12M1/16

    CPC分类号： C12Q1/18 ,  C12Q1/04 ,  G01N33/5695 ,  G01N2333/35 ,  Y10S435/863 ,  Y10S435/864 ,  Y10S435/975

    摘要： A method of speciating and identifying MAI in a specimen comprises placing a paraffin coated slide in a receptacle containing a sterile aqueous solution inoculated with the specimen, analyzing the slide after exposure to the specimen to determine the presence or absence of atypical Mycobacteria, and after the analysis step, if atypical Mycobacteria are determined to be present, performing at least one speciation assay to ascertain if the atypical Mycobacteria are MAI. A related apparatus is also disclosed for speciating and identifying MAI in a specimen comprising a paraffin-wax coated slide, a tube having a sterile aqueous solution contained therein, the tube adapted to hold the slide, and at least one speciation assay means for performing an assay to determine the presence or absence of MAI in the specimen after the specimen is introduced into the tube holding the solution and the slide. An apparatus and method for determining the sensitivity of MAI to different antibiotics and dosages thereof is also provided.

    摘要翻译： 在样品中指定和鉴定MAI的方法包括将石蜡包裹的载玻片放置在含有接种样品的无菌水溶液的容器中，暴露于样品后分析载玻片以确定非典型分枝杆菌的存在或不存在 分析步骤，如果确定非典型分枝杆菌存在，进行至少一种形态分析以确定非典型分枝杆菌是否是MAI。 还公开了一种相关装置，用于指定和鉴定包含石蜡包被的载玻片的试样中的MAI，其中含有无菌水溶液的管，适于保持载玻片的管，以及至少一个形成分析装置，用于执行 在将样品引入保持溶液和载玻片的管中之后，测定样品中是否存在MAI。 还提供了用于确定MAI对不同抗生素及其剂量的敏感性的装置和方法。