公开(公告)号：US20120229891A1

公开(公告)日：2012-09-13

申请号：US13418097

申请日：2012-03-12

Applicant: Zhaowei Liu ,  Feifei Wei

Inventor： Zhaowei Liu ,  Feifei Wei

IPC: G02B13/14

CPC classification number: G02B5/008 ,  G01N21/553 ,  G01N21/6458 ,  G01N21/648 ,  G01N2201/062 ,  G01N2201/0628

Abstract: Plasmonic condensers for generating surface plasmon at an evanescent wave surface can include a substrate layer, a metal layer comprising the evanescent wave surface; and a media layer disposed between the metal layer and the substrate layer. The media layer can be active or passive and can include a source of radiation that interacts with the metal layer to create surface plasmons that are not substantially optically detectable as far field radiation until an interfering object is brought into proximity with the evanescent wave surface. When an interfering object such as a sample or specimen is brought into proximity with the evanescent wave surface, it causes coupling of at least some of the surface plasmons into propagating radiation detectable by an objective lens. Systems, methods, and the like are disclosed, as are features of a plasmonic meta-materials illuminator.

Abstract translation: 用于在ev逝波表面产生表面等离子体激元的等离子体冷凝器可以包括基底层，包含ev逝波面的金属层; 以及设置在金属层和基底层之间的介质层。 介质层可以是有源的或无源的，并且可以包括与金属层相互作用的辐射源，以产生表面等离子体，这些表面等离激元不是远场光辐射，直到干扰物体接近于ev逝波面。 当诸如样品或样品的干扰物体与ev逝波表面接近时，其使至少一些表面等离子体激元耦合到由物镜可检测的传播辐射。 公开了系统，方法等，以及等离子体激元间材料照明器的特征。

公开(公告)号：US20060029949A1

公开(公告)日：2006-02-09

申请号：US11103356

申请日：2005-04-11

Applicant: Zhaowei Liu ,  Thomas Kane

Inventor： Zhaowei Liu ,  Thomas Kane

IPC: C12Q1/68

CPC classification number: C12Q1/68 ,  C12Q2565/125 ,  C12Q2565/102 ,  C12Q2545/101

Abstract: According to a method of determining a size of a sample polynucleotide, a sample polynucleotide is subjected to electrophoresis in the presence of a fluorescent compound having a first fluorescence spectrum. Detection of light of the first fluorescence spectrum is indicative of the presence of the sample polynucleotide. One or more size standards are also subjected to electrophoresis, optionally in the presence of the sample polynucleotide. If more than one size standard is used, the different size standards typically have different mobilities. The size standards are generally essentially or completely free of polynucleotides. Migration coordinates, e.g., migration times, of the sample polynucleotide and size standard(s) are determined. A size of the sample polynucleotide can be determined using the migration coordinate of the sample polynucleotide and the migration coordinate(s) of the size standard(s).

Abstract translation: 根据测定样品多核苷酸的大小的方法，在具有第一荧光光谱的荧光化合物的存在下对样品多核苷酸进行电泳。 第一荧光光谱的光的检测指示样品多核苷酸的存在。 也可以在样品多核苷酸的存在下，一个或多个尺寸标准品进行电泳。 如果使用多于一个尺寸的标准，则不同尺寸的标准通常具有不同的移动性。 大小标准通常基本上或完全不含多核苷酸。 测定样品多核苷酸的迁移坐标，例如迁移时间和大小标准。 可以使用样品多核苷酸的迁移坐标和尺寸标准的迁移坐标来确定样品多核苷酸的大小。

公开(公告)号：US09304234B2

公开(公告)日：2016-04-05

申请号：US13418097

申请日：2012-03-12

Applicant: Zhaowei Liu ,  Feifei Wei

Inventor： Zhaowei Liu ,  Feifei Wei

IPC: G02B5/00 ,  G01N21/552 ,  G01N21/64

CPC classification number: G02B5/008 ,  G01N21/553 ,  G01N21/6458 ,  G01N21/648 ,  G01N2201/062 ,  G01N2201/0628

Abstract: Plasmonic condensers for generating surface plasmon at an evanescent wave surface can include a substrate layer, a metal layer comprising the evanescent wave surface; and a media layer disposed between the metal layer and the substrate layer. The media layer can be active or passive and can include a source of radiation that interacts with the metal layer to create surface plasmons that are not substantially optically detectable as far field radiation until an interfering object is brought into proximity with the evanescent wave surface. When an interfering object such as a sample or specimen is brought into proximity with the evanescent wave surface, it causes coupling of at least some of the surface plasmons into propagating radiation detectable by an objective lens. Systems, methods, and the like are disclosed, as are features of a plasmonic meta-materials illuminator.

Abstract translation: 用于在ev逝波表面产生表面等离子体激元的等离子体冷凝器可以包括基底层，包含ev逝波面的金属层; 以及设置在金属层和基底层之间的介质层。 介质层可以是有源的或无源的，并且可以包括与金属层相互作用的辐射源，以产生表面等离子体，这些表面等离激元不是远场光辐射，直到干扰物体接近于ev逝波面。 当诸如样品或样品的干扰物体与ev逝波表面接近时，其使至少一些表面等离子体激元耦合到由物镜可检测的传播辐射。 公开了系统，方法等，以及等离子体激元间材料照明器的特征。

公开(公告)号：US07303879B2

公开(公告)日：2007-12-04

申请号：US10910256

申请日：2004-08-02

Applicant: Zhaowei Liu ,  Yiqiong Wu

Inventor： Zhaowei Liu ,  Yiqiong Wu

IPC: C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12Q1/6827 ,  C12Q1/6858 ,  C12Q2565/131

Abstract: A method for determining a frequency of single nucleotide polymorphism (SNP) within genomic DNA includes providing genomic DNA of each of a plurality of different organisms. The genomic DNA of each organism includes first and second portions, e.g., first and second strands. First and second amplicons are prepared from the genomic DNA of each organism. The first amplicon corresponds to the first portion of the genomic DNA and the second amplicon corresponds to the second portion of the genomic DNA.A plurality of duplexes is prepared from the first and second amplicons of the genomic DNA of each organism. At least some of the duplexes include a portion of one of the first amplicons and a portion of one of the second amplicons.The duplexes are subjected to temperature gradient electrophoresis to obtain first electrophoresis data indicative of the rate of SNP at a first location in the genomic DNA of the plurality of organisms.

Abstract translation: 用于确定基因组DNA内的单核苷酸多态性（SNP）的频率的方法包括提供多种不同生物体中的每一种的基因组DNA。 每个生物体的基因组DNA包括第一和第二部分，例如第一和第二链。 从每个生物体的基因组DNA制备第一和第二扩增子。 第一扩增子对应于基因组DNA的第一部分，第二扩增子对应于基因组DNA的第二部分。

公开(公告)号：US08836948B2

公开(公告)日：2014-09-16

申请号：US13146550

申请日：2010-01-28

Applicant: Zhaowei Liu

Inventor： Zhaowei Liu

IPC: G01B9/02 ,  G01N21/55 ,  G02B27/60 ,  G02B5/00 ,  G01N21/64 ,  G01B11/24 ,  G01B9/04 ,  G02B21/16

CPC classification number: G02B21/16 ,  G01B9/04 ,  G01B11/2441 ,  G01N21/6458 ,  G01N21/648 ,  G01N2201/0635 ,  G01N2201/0675 ,  G02B5/008 ,  G02B27/60

Abstract: Disclosed are systems, apparatus, methods and devices, including a method that includes generating two or more sequential surface plasmon interference patterns, at least one of the two or more sequential surface plasmon interference patterns being different from another of the two or more sequential surface plasmon interference patterns, and capturing respective images of a specimen resulting from the interference patterns. Also disclosed is a method that includes generating two or more sequential optical interference patterns, at least one of the two or more sequential optical interference patterns being different from another of the interference patterns, and removing from each of the generated interference patterns, using a beam stopper, a corresponding zero-order diffraction light component included in the respective generated patterns to obtain resultant corresponding two or more sequential optical interference patterns, directed at a specimen, with missing respective zero-order light components.

Abstract translation: 公开了一种系统，装置，方法和装置，包括一种方法，包括产生两个或多个顺序表面等离子体激元干涉图案，两个或更多个顺序表面等离子体激元干涉图案中的至少一个不同于两个或多个顺序表面等离子体激元 干涉图案，并捕获由干涉图案产生的样本的各个图像。 还公开了一种方法，其包括生成两个或多个顺序光学干涉图案，所述两个或更多个顺序光学干涉图案中的至少一个与干涉图案中的另一个不同，以及使用光束从每个所生成的干涉图案中移除 阻挡器，包括在各个生成的图案中的对应的零级衍射光分量，以获得指向样本的相应的两个或更多个顺序的光学干涉图案，缺少相应的零级光分量。

公开(公告)号：US20120069344A1

公开(公告)日：2012-03-22

申请号：US13146550

申请日：2010-01-28

Applicant: Zhaowei Liu

Inventor： Zhaowei Liu

IPC: G01B9/02

CPC classification number: G02B21/16 ,  G01B9/04 ,  G01B11/2441 ,  G01N21/6458 ,  G01N21/648 ,  G01N2201/0635 ,  G01N2201/0675 ,  G02B5/008 ,  G02B27/60

Abstract: Disclosed are systems, apparatus, methods and devices, including a method that includes generating two or more sequential surface plasmon interference patterns, at least one of the two or more sequential surface plasmon interference patterns being different from another of the two or more sequential surface plasmon interference patterns, and capturing respective images of a specimen resulting from the interference patterns. Also disclosed is a method that includes generating two or more sequential optical interference patterns, at least one of the two or more sequential optical interference patterns being different from another of the interference patterns, and removing from each of the generated interference patterns, using a beam stopper, a corresponding zero-order diffraction light component included in the respective generated patterns to obtain resultant corresponding two or more sequential optical interference patterns, directed at a specimen, with missing respective zero-order light components.

Abstract translation: 公开了一种系统，装置，方法和装置，包括一种方法，包括产生两个或多个顺序表面等离子体激元干涉图案，两个或更多个顺序表面等离子体激元干涉图案中的至少一个不同于两个或多个顺序表面等离子体激元 干涉图案，并捕获由干涉图案产生的样本的各个图像。 还公开了一种方法，其包括生成两个或多个顺序光学干涉图案，所述两个或更多个顺序光学干涉图案中的至少一个与干涉图案中的另一个不同，以及使用光束从每个所生成的干涉图案中移除 阻挡器，包括在各个生成的图案中的对应的零级衍射光分量，以获得指向样本的相应的两个或更多个顺序的光学干涉图案，缺少相应的零级光分量。

公开(公告)号：US20060088853A1

公开(公告)日：2006-04-27

申请号：US11185916

申请日：2005-07-20

Applicant: Zhaowei Liu ,  Yiqiong Wu

Inventor： Zhaowei Liu ,  Yiqiong Wu

IPC: C12Q1/68

CPC classification number: C12Q1/6816 ,  C12Q1/68 ,  C12Q2565/125 ,  C12Q2537/107 ,  C12Q2527/15 ,  C12Q2525/204

Abstract: The present invention relates to a method of determining whether a length of a first polynucleotide is equal to (a) a length of a second polynucleotide or (b) a length of a third polynucleotide. A sample including the first polynucleotide is provided. A first portion of the sample is combined with an amount of the second polynucleotide to form a first mixture. A second portion of the sample is combined with an amount of the third polynucleotide to form a second, different mixture. The second polynucleotide includes at least 1 additional base than the third polynucleotide. The at least 1 additional base is located intermediate terminal ends of the second polynucleotide. First duplexes including the first polynucleotide and the second polynucleotide are prepared. Second duplexes including the first polynucleotide and the third polynucleotide are prepared. The first and second duplexes are subjected to temperature gradient electrophoresis to obtain electrophoresis data. The electrophoresis data is analyzed to determine whether the length of the first polynucleotide is equal to (a) the length of the second polynucleotide or (b) the length of the third polynucleotide.

Abstract translation: 本发明涉及确定第一多核苷酸的长度是否等于（a）第二多核苷酸的长度或（b）第三多核苷酸的长度的方法。 提供了包含第一多核苷酸的样品。 将样品的第一部分与一定量的第二多核苷酸组合以形成第一混合物。 样品的第二部分与一定量的第三多核苷酸组合以形成第二种不同的混合物。 第二个多核苷酸包括比第三个多核苷酸至少1个碱基。 至少另外1个碱基位于第二多核苷酸的中间末端。 制备包括第一多核苷酸和第二多核苷酸的第一双链体。 制备包括第一多核苷酸和第三多核苷酸的第二双链体。 对第一和​​第二双链体进行温度梯度电泳以获得电泳数据。 分析电泳数据以确定第一多核苷酸的长度是否等于（a）第二多核苷酸的长度，或（b）第三多核苷酸的长度。

公开(公告)号：US06872530B2

公开(公告)日：2005-03-29

申请号：US10128334

申请日：2002-04-24

Applicant: Zhaowei Liu

Inventor： Zhaowei Liu

IPC: C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC classification number: C12Q1/6827 ,  C12Q2565/125 ,  C12Q2527/15 ,  C12Q2527/107

Abstract: The present invention relates to a peptide nucleic acid probe-based method for generating data indicative of the presence of a nucleotide polymorphism, mutation, or methylated cytosine in a nucleotide containing compound. A peptide nucleic acid probe (PNAP) is subjected to temperature gradient electrophoresis in the presence of a nucleotide containing compound. The PNAP is irradiated to generate a spectroscopic signal. The spectroscopic signal is converted into data suitable for determining the presence of the nucleotide polymorphism or the mutation in the nucleotide-containing compound.

Abstract translation: 本发明涉及基于肽核酸探针的方法，用于产生指示核苷酸含有化合物中核苷酸多态性，突变或甲基化胞嘧啶存在的数据。 肽核酸探针（PNAP）在含有核苷酸的化合物存在下进行温度梯度电泳。 照射PNAP以产生光谱信号。 光谱信号被转换为适合于确定核苷酸多态性的存在或含有核苷酸的化合物中的突变的数据。

公开(公告)号：US09151891B2

公开(公告)日：2015-10-06

申请号：US13578665

申请日：2011-02-14

Applicant: Changbao Ma ,  Zhaowei Liu

Inventor： Changbao Ma ,  Zhaowei Liu

IPC: G02B6/32 ,  G02B6/122 ,  B82Y20/00 ,  G02B1/00 ,  G02B6/10 ,  H01Q15/02 ,  H01Q15/00

CPC classification number: G02B6/1226 ,  B82Y20/00 ,  G02B1/002 ,  G02B6/107 ,  G02F2203/10 ,  H01Q15/0086 ,  H01Q15/02

Abstract: Devices based on metamaterial structures to guide and manipulate light, other electromagnetic radiation and acoustic waves. For example, a lens can include a metamaterial structure comprising nano structures of metallic and dielectric materials; and a plasmonic waveguide coupler formed over the metamaterial structure for coupling electromagnetic radiation to or from metamaterial structure. The metamaterial structure has an anisotropic structure and the plasmonic waveguide coupler is structured to include metal and non-metal parts to support surface plasmon polaritons and to cause different phase delays at different locations of an interface with the metamaterial structure in a way that the metamaterial structure and the plasmonic waveguide coupler effect a lens for performing a Fourier transform of the electromagnetic radiation coupled between the metamaterial structure and the plasmonic waveguide coupler.

Abstract translation: 基于超材料结构的设备来引导和操纵光，其他电磁辐射和声波。 例如，透镜可以包括包括金属和介电材料的纳米结构的超材料结构; 以及形成在超材料结构上的等离子体激元波导耦合器，用于将电磁辐射耦合到超材料结构或从超材料结构耦合。 超材料结构具有各向异性结构，等离子体激元波导耦合器被构造为包括金属和非金属部分以支持表面等离子体激元极化并且在与超材料结构的界面的不同位置处引起不同的相位延迟，使得超材料结构 并且等离子体激元波导耦合器影响用于对耦合在超材料结构和等离子体激元波导耦合器之间的电磁辐射进行傅里叶变换的透镜。

公开(公告)号：US07175750B2

公开(公告)日：2007-02-13

申请号：US10287826

申请日：2002-11-05

Applicant: Zhiyong Guo ,  Zhaowei Liu ,  Qingbo Li

Inventor： Zhiyong Guo ,  Zhaowei Liu ,  Qingbo Li

IPC: G01N27/447

CPC classification number: G01N27/44773 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q2600/156 ,  G01N27/44704 ,  G01N27/44721 ,  C12Q2565/125 ,  C12Q2545/113 ,  C12Q2527/101 ,  C12Q2565/131 ,  C12Q2527/107

Abstract: The present invention relates to a method for determining the presence of a mutation in a first sample comprising first nucleotides. The reference sample comprising reference nucleotides. The first sample and a reference sample are subjected to electrophoresis in the presence of at least one intercalating dye. During electrophoresis the temperature of the first sample and the reference sample is changed by an amount sufficient to change an electrophoretic mobility of at least one of the first or reference nucleotides. Fluorescence intensity data are obtained. The fluorescence intensity data are indicative of the presence of the first and reference nucleotides. At least one of the first sample or reference samples comprises products resulting from a polymerase chain reaction (PCR), the products not having been desalted prior to electrophoresis.

Abstract translation: 本发明涉及用于确定包含第一核苷酸的第一样品中突变的存在的方法。 参考样品包含参考核苷酸。 第一个样品和参考样品在至少一个插层染料存在下进行电泳。 在电泳期间，第一样品和参考样品的温度改变足以改变至少一个第一个或参考核苷酸的电泳迁移率的量。 获得荧光强度数据。 荧光强度数据表明第一个和参考核苷酸的存在。 第一个样本或参考样本中的至少一个包含由聚合酶链反应（PCR）产生的产物，该产物在电泳之前未脱盐。