摘要:
The invention provides a reagent for diluting a blood sample, comprising water, polyoxyethylene alkyl ether having a hydroxyl value of 52 to 60, and an osmo-regulator for regulating the osmotic pressure of the reagent in the range of 150 to 400 mOsm/kg, as well as a method for measuring the mean corpuscular volume of a blood sample.
摘要:
The invention provides a reagent for diluting a blood sample, comprising water, polyoxyethylene alkyl ether having a hydroxyl value of 52 to 60, and an osmo-regulator for regulating the osmotic pressure of the reagent in the range of 150 to 400 mOsm/kg, as well as a method for measuring the mean corpuscular volume of a blood sample.
摘要:
A reagent container set is disclosed which comprises: a first reagent container for accommodating a first reagent to be used in a sample analyzer, the top part of the first reagent container having a first aspiration opening for inserting a first reagent aspirating tube to aspirate the first reagent; a waste fluid container for accommodating the first reagent used in the sample analyzer as a waste fluid, the top of the waste fluid container having a discharge opening for inserting a waste fluid discharging tube to discharge the waste fluid into the waste fluid container; a box for accommodating the first reagent container and the waste fluid container; and a container holding member for holding the first reagent container and the waste fluid container so that the first aspiration opening and the discharge opening are disposed at predetermined positions, the container holding member being maintained at a predetermined position within the box.
摘要:
A reagent container set is disclosed which comprises: a first reagent container for accommodating a first reagent to be used in a sample analyzer, the top part of the first reagent container having a first aspiration opening for inserting a first reagent aspirating tube to aspirate the first reagent; a waste fluid container for accommodating the first reagent used in the sample analyzer as a waste fluid, the top of the waste fluid container having a discharge opening for inserting a waste fluid discharging tube to discharge the waste fluid into the waste fluid container; a box for accommodating the first reagent container and the waste fluid container; and a container holding member for holding the first reagent container and the waste fluid container so that the first aspiration opening and the discharge opening are disposed at predetermined positions, the container holding member being maintained at a predetermined position within the box.
摘要:
A reagent for partially lysing a cell membrane of a red blood cell infected with a malaria parasite, comprising: a first surfactant which has predetermined lysing ability for the red blood cell membrane; and a second surfactant which has weaker lysing ability than that of the first surfactant; wherein the reagent has pH of 5 to 7 and osmotic pressure of 200 to 300 mOsm/kg·H2O; and wherein the reagent partially lyses the malaria infected red blood cell membrane such that a malaria parasite is retained in the red blood cell and fluorescent dye passes through the cell membrane, is disclosed. A reagent for detecting malaria infected red blood cells, and a sample analyzing method for detecting malaria infected red blood cells, are also disclosed.
摘要:
A blood analyzing method is described that includes steps of: preparing a measurement sample by hemolyzing red blood cells in a blood specimen, the measurement sample containing particles; irradiating a light on the measurement sample to detect two or more types of optical information reflecting characters of the particles in the measurement sample, thereby acquiring data indicating light intensities of the respective optical information as a particle data associated with each of the particles; counting white blood cells based on the particle data; outputting the result of counting white blood cells; determining whether malarial parasites are present or not based on the particle data; and outputting the result of determining. A blood analyzer also described.
摘要:
Aqueous blood-sample diluting reagent and method of its use for compelling a morphological change in a blood sample to yield an MCV value assayed at elapsed time after the sample is drawn to be consistent within a diagnostically acceptable range with the original, immediate post-drawing MCV value. Selection of a small amount of a predetermined surfactant added within a limited range of concentration, and of a salt for adjusting osmotic pressure of the sample is thereby determined. The blood sample is treated with an anti-coagulant agent immediately post-drawing, and for assay in a particle analyzer at post-drawing elapsed time is diluted with the reagent solution. The reagent has an osmotic pressure (&pgr;) of approximately 150-400 mOsm/kg and a pH of 6.0-8.5. The surfactant is present in a 0.0005% to 0.5% concentration and has a hydrophile-lipophile balance (HLB) of 10-20.
摘要:
This blood analyzer includes a sample preparation portion preparing a measurement sample free from a labeling substance from a blood sample and a hemolytic agent free from a labeling substance, a light information generation portion generating fluorescent information and at least two types of scattered light information from the measurement sample and a control portion performing a first classification of white blood cells in the measurement sample into at least four groups of monocytes, neutrophils, eosinophils and others on the basis of the fluorescent information and the two types of scattered light information.
摘要:
This blood analyzer includes a sample preparation portion preparing a first measurement sample containing a blood sample and a hemolytic agent and a second measurement sample containing the blood sample, the same hemolytic agent as the hemolytic agent and a staining agent and a control portion classifying white blood cells in the first measurement sample into at least four groups of monocytes, neutrophils, eosinophils and others on the basis of fluorescent information and two types of scattered light information generated by a light information generation portion and classifying blood cells in the second measurement sample into at least malaria-infected red blood cells and others on the basis of fluorescent information and scattered light information generated by the light information generation portion.
摘要:
A reagent for measurement of leukocytes and hemoglobin concentration in the blood includes a cationic surfactant in an amount sufficient to lyse erythrocytes and denature hemoglobin, at least one of the following hemoglobin stabilizers: (a) sulfosalicylic acid, or its salt, in an amount effective for promoting the conversion of hemoglobin into methemoglobin, (b) 0.2 to 10.0 g/L of a water-soluble chelating agent having a nitrogen atom and a carboxyl group, and (c) piperazine, or its salt, in an amount effective for promoting the conversion of hemoglobin into methemoglobin, and a buffer for maintaining pH at 4 to 6.