公开(公告)号：US20240240153A1

公开(公告)日：2024-07-18

申请号：US18391867

申请日：2023-12-21

Applicant: Vertex Pharmaceuticals Incorporated

Inventor： Yeh-Chuin POH ,  George HARB ,  Felicia J. PAGLIUCA

IPC: C12N5/071 ,  C12N5/00

CPC classification number: C12N5/0676 ,  C12N5/0031 ,  C12N2506/22 ,  C12N2509/10 ,  C12N2527/00

Abstract: The present application discloses cell clusters resembling the function and characteristics of endogenous pancreatic islets, and methods for making and using such cell clusters.

公开(公告)号：US20220235327A1

公开(公告)日：2022-07-28

申请号：US17669874

申请日：2022-02-11

Applicant: Vertex Pharmaceuticals Incorporated

Inventor： George HARB ,  Lillian YE

IPC: C12N5/071 ,  A61K35/39 ,  A61P3/10

Abstract: In some aspects, disclosed herein are methods and compositions for generating enterochromaffin cells. In some aspects, the methods and compositions disclosed herein relate to use of an inhibitor of IL-4/JAK3 signaling pathway. In other aspects, disclosed herein are cell compositions, pharmaceutical compositions, and medical devices that relate to pancreatic cells that are generated according to the methods disclosed herein.

公开(公告)号：US20240358767A1

公开(公告)日：2024-10-31

申请号：US18707756

申请日：2022-11-07

Applicant: VERTEX PHARMACEUTICALS INCORPORATED

Inventor： Chunhui XIE ,  George HARB ,  Elizabeth RYU ,  Nicholas TECENO ,  Christopher FARRAR

IPC: A61K35/39 ,  C12N5/071

CPC classification number: A61K35/39 ,  C12N5/0676 ,  C12N2501/117 ,  C12N2501/16 ,  C12N2501/385 ,  C12N2501/72

Abstract: Disclosed herein are compositions and methods related to differentiation of stem cells into pancreatic cells. In some aspects, the methods provided herein relate to generation of pancreatic β cell, α cell, δ cells, and EC cells in vitro in the presence of one or more chemical compounds that inhibit PI3K/Akt/mTOR signaling. In some aspects, the disclosure provides pharmaceutical compositions including the cells generated according to the methods disclosed herein, as well as methods of making use thereof.

公开(公告)号：US20230146780A1

公开(公告)日：2023-05-11

申请号：US18054860

申请日：2022-11-11

Applicant: Vertex Pharmaceuticals Incorporated

Inventor： Felicia PAGLIUCA ,  George HARB ,  Mads GURTLER ,  Austin THIEL ,  Jihad YASIN ,  Evrett THOMPSON

IPC: C12N5/071

CPC classification number: C12N5/0676 ,  C12N2501/11 ,  C12N2501/15 ,  C12N2501/16 ,  C12N2501/999

Abstract: Disclosed herein are compositions and methods of enhancing stem cell differentiation into beta cells with use of one or more epigenetic modification compounds. The present disclosure also relates to compositions and methods of sorting and enriching the differentiated beta cells. The present disclosure also relates to compositions and methods of irradiating cell population for reducing proliferation.

公开(公告)号：US20230137854A1

公开(公告)日：2023-05-04

申请号：US17985746

申请日：2022-11-11

Applicant: Vertex Pharmaceuticals Incorporated

Inventor： Felicia PAGLIUCA ,  George HARB ,  Mads GURTLER ,  Austin THIEL ,  Jihad YASIN ,  Evrett THOMPSON

IPC: C12N5/071

Abstract: Disclosed herein are compositions and methods of enhancing stem cell differentiation into beta cells with use of one or more epigenetic modification compounds. The present disclosure also relates to compositions and methods of sorting and enriching the differentiated beta cells. The present disclosure also relates to compositions and methods of irradiating cell population for reducing proliferation.

公开(公告)号：US20230075375A1

公开(公告)日：2023-03-09

申请号：US18055312

申请日：2022-11-14

Applicant: Vertex Pharmaceuticals Incorporated

Inventor： Felicia J. PAGLIUCA ,  George HARB ,  Lillian YE

IPC: C12N5/071

Abstract: Provided herein are methods of producing β cells and precursors thereof utilizing a Wnt signaling inhibitor or PKC activator, or both. Also provided herein are in vitro cultures comprising said cells, methods of treating a subject with a disease characterized by high blood sugar levels over a prolonged period of time by administering said cells, and devices for encapsulating said cells.

公开(公告)号：US20240271100A1

公开(公告)日：2024-08-15

申请号：US18018787

申请日：2021-07-30

Applicant: VERTEX PHARMACEUTICALS INCORPORATED

Inventor： George HARB ,  Chunhui XIE

IPC: C12N5/071

CPC classification number: C12N5/0677 ,  C12N2501/115 ,  C12N2506/45

Abstract: Disclosed herein are compositions and methods related to differentiation of stem cells into pancreatic endocrine cells. In some aspects, the methods provided herein relate to generation of pancreatic β cell, α cell, δ cells, and EC cells in vitro. In some aspects, the disclosure provides pharmaceutical compositions including the cells generated according to the methods disclosed herein, as well as methods of treatment making use thereof.

公开(公告)号：US20240117319A1

公开(公告)日：2024-04-11

申请号：US18391831

申请日：2023-12-21

Applicant: Vertex Pharmaceuticals Incorporated

Inventor： Yeh-Chuin POH ,  George HARB ,  Felicia J. PAGLIUCA

IPC: C12N5/071 ,  C12N5/00

CPC classification number: C12N5/0676 ,  C12N5/0031 ,  C12N2506/22 ,  C12N2509/10 ,  C12N2527/00

Abstract: The present application discloses cell clusters resembling the function and characteristics of endogenous pancreatic islets, and methods for making and using such cell clusters.

公开(公告)号：US20230332107A1

公开(公告)日：2023-10-19

申请号：US18162129

申请日：2023-01-31

Applicant: Vertex Pharmaceuticals Incorporated

Inventor： Felicia PAGLIUCA ,  George HARB ,  Jason GAGLIA ,  Andrea VERGANI ,  Christopher THANOS

IPC: C12N5/071

CPC classification number: C12N5/0678 ,  C12N2501/15 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/02

Abstract: Disclosed herein are compositions, kits, and methods related to cell therapy for a disease characterized by high blood glucose levels over a long period of time, such as diabetes. In some aspects, the methods provided herein relate to administration of non-native pancreatic cells to subject with a disease characterized by high blood glucose levels over a long period of time, such as diabetes. In some aspects, the disclosure provides pharmaceutical compositions including non-native cells.

公开(公告)号：US20230085395A1

公开(公告)日：2023-03-16

申请号：US18055327

申请日：2022-11-14

Applicant: Vertex Pharmaceuticals Incorporated

Inventor： Felicia J. PAGLIUCA ,  George HARB ,  Lillian YE

IPC: C12N5/071

Abstract: Provided herein are methods of producing β cells and precursors thereof utilizing a Wnt signaling inhibitor or PKC activator, or both. Also provided herein are in vitro cultures comprising said cells, methods of treating a subject with a disease characterized by high blood sugar levels over a prolonged period of time by administering said cells, and devices for encapsulating said cells.