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公开(公告)号：US20080127650A1
公开(公告)日：2008-06-05
申请号：US12011239
申请日：2008-01-24
CPC分类号： C12M99/00 , B82B1/00 , B82B3/00 , B82Y30/00 , B82Y40/00 , C07K14/4716 , H02N11/006 , Y10S977/729 , Y10T29/4984 , Y10T156/10
摘要： A molecular motor in which multiple concentric cylinders (or nested cones) rotate around a common longitudinal axis. Opposing complementary surfaces of the cylinders or cones are coated with complementary motor protein pairs (such as actin and myosin). The actin and myosin interact with one another in the presence of ATP to rotate the cylinders or cones relative to one another, and this rotational energy is harnessed to produce work. The concentration of ATP and the number of nested cylinders or cones can be used to control the rotational speed of the motor. The length of the cylinders can also be used to control the power generated by the motor. In another embodiment, the molecular motor includes at least two annular substrates wherein one annular substrate is coated with a first motor protein and the other annular substrate is coated with a second motor protein. The first and second motor proteins interact with each other to move the second annular relative to the first annular substrate.
摘要翻译： 一个分子马达，其中多个同心圆筒（或嵌套圆锥体）围绕共同的纵向轴线旋转。 圆柱体或锥体的互补表面涂覆有互补的运动蛋白质对（如肌动蛋白和肌球蛋白）。 肌动蛋白和肌球蛋白在ATP存在下相互作用以相对于彼此旋转圆柱体或锥体，并且利用该旋转能量来产生作用。 ATP的浓度和嵌套圆柱体或锥体的数量可用于控制电机的转速。 气缸的长度也可用于控制电机产生的电力。 在另一个实施例中，分子马达包括至少两个环形基底，其中一个环形基底涂覆有第一运动蛋白，另一个环形基底涂覆有第二运动蛋白。 第一和第二运动蛋白相互作用以相对于第一环形基底移动第二环状物。
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公开(公告)号：US20120094365A1
公开(公告)日：2012-04-19
申请号：US13306774
申请日：2011-11-29
CPC分类号： C12M99/00 , B82B1/00 , B82B3/00 , B82Y30/00 , B82Y40/00 , C07K14/4716 , H02N11/006 , Y10S977/729 , Y10T29/4984 , Y10T156/10
摘要： A molecular motor in which multiple concentric cylinders (or nested cones) rotate around a common longitudinal axis. Opposing complementary surfaces of the cylinders or cones are coated with complementary motor protein pairs (such as actin and myosin). The actin and myosin interact with one another in the presence of ATP to rotate the cylinders or cones relative to one another, and this rotational energy is harnessed to produce work. The length of the cylinders can also be used to control the power generated by the motor. In another embodiment, the molecular motor includes at least two annular substrates wherein one annular substrate is coated with a first motor protein and the other annular substrate is coated with a second motor protein. The first and second motor proteins interact with each other to move the second annular relative to the first annular substrate.
摘要翻译： 一个分子马达，其中多个同心圆柱体（或嵌套圆锥体）围绕共同的纵向轴线旋转。 圆柱体或锥体的互补表面涂覆有互补的运动蛋白质对（如肌动蛋白和肌球蛋白）。 肌动蛋白和肌球蛋白在ATP存在下相互作用以相对于彼此旋转圆柱体或锥体，并且利用该旋转能量来产生作用。 气缸的长度也可用于控制电机产生的电力。 在另一个实施例中，分子马达包括至少两个环形基底，其中一个环形基底涂覆有第一运动蛋白，另一个环形基底涂覆有第二运动蛋白。 第一和第二运动蛋白相互作用以相对于第一环形基底移动第二环状物。
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公开(公告)号：US20110111975A1
公开(公告)日：2011-05-12
申请号：US12980802
申请日：2010-12-29
CPC分类号： G01N33/542 , C12Q1/6818 , C12Q1/6869 , Y10T436/143333 , C12Q2563/107 , C12Q2525/197 , C12Q2521/30 , C12Q2565/101 , C12Q2535/107
摘要： A nanoprobe for sequencing of nucleic acid molecules is provided, as well as methods for using the nanoprobe. In particular examples, the probe includes a polymerizing agent and one or more molecular linkers that carry a chemical moiety capable of reversibly binding to the template strand of a nucleic acid molecule, without being detached from the linker, by specifically binding with a complementary nucleotide in the target nucleic acid molecule. The reversible binding of the chemical moiety on the linker with a complementary nucleotide in the target nucleic acid molecule is indicated by emission of a characteristic signal that indicates pairing of the chemical moiety on the linker with its complementary nucleotide. An example of such a chemical moiety is a nonhydrolyzable nucleotide analog. In particular examples, the polymerizing agent and the chemical moiety are associated with a tag, such as a donor fluorophore and acceptor fluorophore characteristic of the particular type of chemical moiety.
摘要翻译： 提供了用于核酸分子测序的纳米探针，以及使用纳米探针的方法。 在具体实例中，探针包括聚合剂和一个或多个分子接头，其携带能够可逆地结合核酸分子的模板链的化学部分，而不与接头脱离，通过与互补核苷酸特异性结合 靶核酸分子。 在靶核酸分子中，连接体上的化学部分与互补核苷酸的可逆结合通过发射指示连接体上的化学部分与其互补核苷酸的配对的特征信号来表示。 这种化学部分的实例是不可水解的核苷酸类似物。 在具体实例中，聚合剂和化学部分与特定类型的化学部分特征的标签（例如供体荧光团和受体荧光团）相关联。
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4.发明授权
公开(公告)号：US5867402A
公开(公告)日：1999-02-02
申请号：US494115
申请日：1995-06-23
CPC分类号： G06T7/0012 , C12Q1/68 , C12Q1/6827 , G06F19/22 , G06F19/18
摘要： In accordance with the present invention, binding sites are defined based upon the individual information content of a particular site of interest. Substitutions within the binding site sequences can be analyzed to determine whether the substitution will cause a deleterious mutation or a benign polymorphism. In addition, new binding sites can be identified using individual information content. Further a computer system is described for determining and displaying individual information content of a binding site sequence.
摘要翻译： 根据本发明，基于感兴趣的特定位点的个体信息内容来定义结合位点。 可以分析结合位点序列内的取代以确定取代是否会引起有害突变或良性多态性。 此外，可以使用个人信息内容来识别新的结合位点。 此外，描述了用于确定和显示绑定站点序列的个体信息内容的计算机系统。
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公开(公告)号：US08086432B2
公开(公告)日：2011-12-27
申请号：US12011239
申请日：2008-01-24
IPC分类号： G06G7/58
CPC分类号： C12M99/00 , B82B1/00 , B82B3/00 , B82Y30/00 , B82Y40/00 , C07K14/4716 , H02N11/006 , Y10S977/729 , Y10T29/4984 , Y10T156/10
摘要： A molecular motor in which multiple concentric cylinders (or nested cones) rotate around a common longitudinal axis. Opposing complementary surfaces of the cylinders or cones are coated with complementary motor protein pairs (such as actin and myosin). The actin and myosin interact with one another in the presence of ATP to rotate the cylinders or cones relative to one another, and this rotational energy is harnessed to produce work. The concentration of ATP and the number of nested cylinders or cones can be used to control the rotational speed of the motor. The length of the cylinders can also be used to control the power generated by the motor. In another embodiment, the molecular motor includes at least two annular substrates wherein one annular substrate is coated with a first motor protein and the other annular substrate is coated with a second motor protein. The first and second motor proteins interact with each other to move the second annular relative to the first annular substrate.
摘要翻译： 一个分子马达，其中多个同心圆柱体（或嵌套圆锥体）围绕共同的纵向轴线旋转。 圆柱体或锥体的互补表面涂覆有互补的运动蛋白质对（如肌动蛋白和肌球蛋白）。 肌动蛋白和肌球蛋白在ATP存在下相互作用以相对于彼此旋转圆柱体或锥体，并且利用该旋转能量来产生作用。 ATP的浓度和嵌套圆柱体或锥体的数量可用于控制电机的转速。 气缸的长度也可用于控制电机产生的电力。 在另一个实施例中，分子马达包括至少两个环形基底，其中一个环形基底涂覆有第一运动蛋白，另一个环形基底涂覆有第二运动蛋白。 第一和第二运动蛋白相互作用以相对于第一环形基底移动第二环状物。
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公开(公告)号：US20080299565A1
公开(公告)日：2008-12-04
申请号：US12095973
申请日：2006-12-12
CPC分类号： G01N33/542 , C12Q1/6818 , C12Q1/6869 , Y10T436/143333 , C12Q2563/107 , C12Q2525/197 , C12Q2521/30 , C12Q2565/101 , C12Q2535/107
摘要： A nanoprobe for sequencing of nucleic acid molecules is provided, as well as methods for using the nanoprobe. In particular examples, the probe includes a polymerizing agent and one or more molecular linkers that carry a chemical moiety capable of reversibly binding to the template strand of a nucleic acid molecule, without being detached from the linker, by specifically binding with a complementary nucleotide in the target nucleic acid molecule. The reversible binding of the chemical moiety on the linker with a complementary nucleotide in the target nucleic acid molecule is indicated by emission of a characteristic signal that indicates pairing of the chemical moiety on the linker with its complementary nucleotide. An example of such a chemical moiety is a nonhydrolyzable nucleotide analog. In particular examples, the polymerizing agent and the chemical moiety are associated with a tag, such as a donor fluorophore and acceptor fluorophore characteristic of the particular type of chemical moiety.
摘要翻译： 提供了用于核酸分子测序的纳米探针，以及使用纳米探针的方法。 在具体实例中，探针包括聚合剂和一个或多个分子接头，其携带能够可逆地结合核酸分子的模板链的化学部分，而不与接头脱离，通过与互补核苷酸特异性结合 靶核酸分子。 在靶核酸分子中，连接体上的化学部分与互补核苷酸的可逆结合通过发射指示连接体上的化学部分与其互补核苷酸的配对的特征信号来表示。 这种化学部分的实例是不可水解的核苷酸类似物。 在具体实例中，聚合剂和化学部分与特定类型的化学部分特征的标签（例如供体荧光团和受体荧光团）相关联。
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公开(公告)号：US07349834B2
公开(公告)日：2008-03-25
申请号：US10061377
申请日：2002-02-01
IPC分类号： G06G7/48
CPC分类号： C12M99/00 , B82B1/00 , B82B3/00 , B82Y30/00 , B82Y40/00 , C07K14/4716 , H02N11/006 , Y10S977/729 , Y10T29/4984 , Y10T156/10
摘要： A molecular motor in which multiple concentric cylinders (or nested cones) rotate around a common longitudinal axis. Opposing complementary surfaces of the cylinders or cones are coated with complementary motor protein pairs (such as actin and myosin). The actin and myosin interact with one another in the presence of ATP to rotate the cylinders or cones relative to one another, and this rotational energy is harnessed to produce work. The concentration of ATP and the number of nested cylinders or cones can be used to control the rotational speed of the motor. The length of the cylinders can also be used to control the power generated by the motor. In another embodiment, the molecular motor includes at least two annular substrates wherein one annular substrate is coated with a first motor protein and the other annular substrate is coated with a second motor protein. The first and second motor proteins interact with each other to move the second annular relative to the first annular substrate.
摘要翻译： 一个分子马达，其中多个同心圆柱体（或嵌套圆锥体）围绕共同的纵向轴线旋转。 圆柱体或锥体的互补表面涂覆有互补的运动蛋白质对（如肌动蛋白和肌球蛋白）。 肌动蛋白和肌球蛋白在ATP存在下相互作用，以相对于彼此旋转圆柱体或锥体，并利用该旋转能量来产生作用。 ATP的浓度和嵌套圆柱体或锥体的数量可用于控制电机的转速。 气缸的长度也可用于控制电机产生的电力。 在另一个实施例中，分子马达包括至少两个环形基底，其中一个环形基底涂覆有第一运动蛋白，另一个环形基底涂覆有第二运动蛋白。 第一和第二运动蛋白相互作用以相对于第一环形基底移动第二环状物。
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公开(公告)号：US20110008794A1
公开(公告)日：2011-01-13
申请号：US12886686
申请日：2010-09-21
申请人： Thomas D. Schneider
发明人： Thomas D. Schneider
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6818 , C12Q2563/107 , C12Q2537/143
摘要： A method and device is disclosed for high speed, automated sequencing of nucleic acid molecules. A nucleic acid molecule to be sequenced is exposed to a polymerase in the presence of nucleotides which are to be incorporated into a complementary nucleic acid strand. The polymerase carries a donor fluorophore, and each type of nucleotide (e.g. A, T/U, C and G) carries a distinguishable acceptor fluorophore characteristic of the particular type of nucleotide. As the polymerase incorporates individual nucleic acid molecules into a complementary strand, a laser continuously irradiates the donor fluorophore, at a wavelength that causes it to emit an emission signal (but the laser wavelength does not stimulate the acceptor fluorophore). In particular embodiments, no laser is needed if the donor fluorophore is a luminescent molecule or is stimulated by one. The emission signal from the polymerase is capable of stimulating any of the donor fluorophores (but not acceptor fluorophores), so that as a nucleotide is added by the polymerase, the acceptor fluorophore emits a signal associated with the type of nucleotide added to the complementary strand. The series of emission signals from the acceptor fluorophores is detected, and correlated with a sequence of nucleotides that correspond to the sequence of emission signals.
摘要翻译： 公开了用于核酸分子的高速，自动测序的方法和装置。 待测序的核酸分子在待并入互补核酸链的核苷酸的存在下暴露于聚合酶。 聚合酶携带供体荧光团，并且每种类型的核苷酸（例如A，T / U，C和G）携带特定类型核苷酸特征的可区别的受体荧光团。 当聚合酶将单个核酸分子结合到互补链中时，激光以使得其发射发射信号（但激光波长不刺激受体荧光团）的波长连续照射供体荧光团。 在具体实施方案中，如果供体荧光团是发光分子或被一个激发的，则不需要激光。 来自聚合酶的发射信号能够刺激任何供体荧光团（但不是受体荧光团），使得当通过聚合酶加入核苷酸时，受体荧光团发射与添加到互补链的核苷酸类型相关联的信号 。 检测来自受体荧光团的一系列发射信号，并与与发射信号序列相对应的核苷酸序列相关。
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公开(公告)号：US08344121B2
公开(公告)日：2013-01-01
申请号：US11638160
申请日：2006-12-12
CPC分类号： G01N33/542 , C12Q1/6818 , C12Q1/6869 , Y10T436/143333 , C12Q2563/107 , C12Q2525/197 , C12Q2521/30 , C12Q2565/101 , C12Q2535/107
摘要： The disclosure provides probes for one or more target molecules. In particular examples, the probes include a molecular linker and first and second functional groups linked and spaced by the molecular linker, wherein the functional groups are capable of interacting with one another or with the target biomolecule in a predetermined reaction, and wherein the molecular linker maintains the first and second functional groups sufficiently spaced from one another such that the functional groups do not substantially interact in an absence of the target biomolecule. In the presence of the target biomolecule the functional groups interact (with each other, with the target biomolecule, or both), and in some examples a detectable signal is produced. In some examples, the functional groups can detect or modify a target molecule. Also provided are methods of using the probes, for example to detect or modify a target molecule.
摘要翻译： 本公开提供了一种或多种靶分子的探针。 在具体实例中，探针包括分子接头和由分子接头连接和隔开的第一和第二官能团，其中官能团能够在预定反应中彼此相互作用或与目标生物分子相互作用，并且其中分子接头 保持第一和第二官能团彼此充分间隔，使得官能团在不存在目标生物分子的情况下基本上不起作用。 在目标生物分子的存在下，官能团相互作用（彼此，与目标生物分子或两者），并且在一些实例中产生可检测的信号。 在一些实例中，官能团可以检测或修饰靶分子。 还提供了使用探针的方法，例如检测或修饰靶分子。
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公开(公告)号：US20090061447A1
公开(公告)日：2009-03-05
申请号：US12196139
申请日：2008-08-21
申请人： Thomas D. Schneider
发明人： Thomas D. Schneider
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6818 , C12Q2563/107 , C12Q2537/143
摘要： A method and device is disclosed for high speed, automated sequencing of nucleic acid molecules. A nucleic acid molecule to be sequenced is exposed to a polymerase in the presence of nucleotides which are to be incorporated into a complementary nucleic acid strand. The polymerase carries a donor fluorophore, and each type of nucleotide (e.g. A, T/U, C and G) carries a distinguishable acceptor fluorophore characteristic of the particular type of nucleotide. As the polymerase incorporates individual nucleic acid molecules into a complementary strand, a laser continuously irradiates the donor fluorophore, at a wavelength that causes it to emit an emission signal (but the laser wavelength does not stimulate the acceptor fluorophore). In particular embodiments, no laser is needed if the donor fluorophore is a luminescent molecule or is stimulated by one. The emission signal from the polymerase is capable of stimulating any of the donor fluorophores (but not acceptor fluorophores), so that as a nucleotide is added by the polymerase, the acceptor fluorophore emits a signal associated with the type of nucleotide added to the complementary strand. The series of emission signals from the acceptor fluorophores is detected, and correlated with a sequence of nucleotides that correspond to the sequence of emission signals.
摘要翻译： 公开了用于核酸分子的高速，自动测序的方法和装置。 待测序的核酸分子在待并入互补核酸链的核苷酸的存在下暴露于聚合酶。 聚合酶携带供体荧光团，并且每种类型的核苷酸（例如A，T / U，C和G）携带特定类型核苷酸特征的可区别的受体荧光团。 当聚合酶将单个核酸分子结合到互补链中时，激光以使得其发射发射信号（但激光波长不刺激受体荧光团）的波长连续照射供体荧光团。 在具体实施方案中，如果供体荧光团是发光分子或被一个激发的，则不需要激光。 来自聚合酶的发射信号能够刺激任何供体荧光团（但不是受体荧光团），使得当通过聚合酶加入核苷酸时，受体荧光团发射与添加到互补链的核苷酸类型相关联的信号 。 检测来自受体荧光团的一系列发射信号，并与与发射信号序列相对应的核苷酸序列相关。
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