公开(公告)号：US09162939B2

公开(公告)日：2015-10-20

申请号：US14621340

申请日：2015-02-12

申请人： The Regents of the University of California

发明人： Ramesh Jasti ,  Carolyn Bertozzi

IPC分类号： C07C13/273 ,  C07C1/22 ,  C07C15/14 ,  C07C41/16 ,  C07C41/30 ,  C07C43/192 ,  C07C43/21 ,  C07C41/01 ,  C07F5/02

CPC分类号： C07C1/22 ,  C07C15/14 ,  C07C41/01 ,  C07C41/16 ,  C07C41/30 ,  C07C43/192 ,  C07C43/21 ,  C07C2523/04 ,  C07C2603/92 ,  C07F5/025

摘要： The present invention provides cycloparaphenylene compounds, their macrocyclic precursors, and methods for making the compounds. The cycloparaphenylene compounds can be used to prepare armchair carbon nanotubes.

公开(公告)号：US08846866B2

公开(公告)日：2014-09-30

申请号：US13708528

申请日：2012-12-07

申请人： The Regents of the University of California

发明人： Isaac S. Carrico ,  Brian L. Carlson ,  Peng Wu ,  Carolyn Bertozzi

IPC分类号： C07K1/00 ,  C07K16/00 ,  A61L33/00 ,  C07K1/13 ,  C07K1/107 ,  C07K14/00 ,  C12P21/00 ,  C07K16/46 ,  C12N9/96

CPC分类号： C07K16/00 ,  A61K38/1825 ,  A61K38/215 ,  A61K38/37 ,  A61K38/45 ,  A61K39/00 ,  C07K1/006 ,  C07K1/1072 ,  C07K1/13 ,  C07K14/00 ,  C07K14/35 ,  C07K14/565 ,  C07K16/46 ,  C07K2317/24 ,  C07K2317/40 ,  C07K2317/52 ,  C07K2317/622 ,  C12N9/13 ,  C12N9/96 ,  C12P21/00 ,  C12P21/005 ,  C12Y208/02

摘要： The invention features compositions and methods for site-specific modification of proteins by incorporation of an aldehyde tag. Enzymatic modification at a sulfatase motif of the aldehyde tag through action of a formylglycine generating enzyme (FGE) generates a formylglycine (FGly) residue. The aldehyde moiety of FGly residue can be exploited as a chemical handle for site-specific attachment of a moiety of interest to a polypeptide.

公开(公告)号：US20180100181A1

公开(公告)日：2018-04-12

申请号：US15566594

申请日：2016-04-15

申请人： The Regents of the University of California

发明人： Peter Robinson ,  Cheng-Ting Tsai ,  Carolyn Bertozzi

IPC分类号： C12Q1/6804 ,  G01N33/543 ,  C12Q1/686 ,  C12Q1/6806

CPC分类号： C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q2525/117 ,  C12Q2525/155 ,  C12Q2525/197 ,  C12Q2531/113 ,  G01N33/54306

摘要： Methods are provided for detecting antigen binding agents in samples. Aspects of the methods include detection of the aggregation of antigen binding agents with polynucleotide-bound antigens by sensitive proximity-based association of the antigen-bound polynucleotides. Aspects of the methods also include methods for the detection of such proximity-based association through nucleic acid amplification. In addition, compositions, e.g., reagents, kits, and devices, useful in practicing various embodiments of the methods are provided.

公开(公告)号：US12054768B2

公开(公告)日：2024-08-06

申请号：US17474424

申请日：2021-09-14

申请人： The Regents of the University of California

发明人： Peter Robinson ,  Cheng-Ting Tsai ,  Carolyn Bertozzi

IPC分类号： C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/686 ,  G01N33/543

CPC分类号： C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/686 ,  G01N33/54306 ,  C12Q2525/117 ,  C12Q2525/155 ,  C12Q2525/197 ,  C12Q2531/113

摘要： Methods are provided for detecting antigen binding agents in samples. Aspects of the methods include detection of the aggregation of antigen binding agents with polynucleotide-bound antigens by sensitive proximity-based association of the antigen-bound polynucleotides. Aspects of the methods also include methods for the detection of such proximity-based association through nucleic acid amplification. In addition, compositions, e.g., reagents, kits, and devices, useful in practicing various embodiments of the methods are provided.

公开(公告)号：US09969970B2

公开(公告)日：2018-05-15

申请号：US15057604

申请日：2016-03-01

申请人： The Regents of the University of California

发明人： Shih-Chia Hsiao ,  Matthew B. Francis ,  Carolyn Bertozzi ,  Richard Mathies ,  Ravi Chandra ,  Erik Douglas ,  Amy Twite ,  Nicholas Toriello ,  Hiroaki Onoe

IPC分类号： C12N5/07 ,  C07H21/00 ,  C12N5/0789 ,  C12N5/04 ,  C12N5/00 ,  A61K47/69

CPC分类号： C12N5/0006 ,  A61K47/6901

摘要： The present invention provides conjugates of DNA and cells by linking the DNA to a native functional group on the cell surface. The cells can be without cell walls or can have cell walls. The modified cells can be linked to a substrate surface and used in assay or bioreactors.

公开(公告)号：US20160319238A1

公开(公告)日：2016-11-03

申请号：US15057604

申请日：2016-03-01

申请人： The Regents of the University Of California

发明人： Shih-Chia Hsiao ,  Matthew B. Francis ,  Carolyn Bertozzi ,  Richard Mathies ,  Ravi Chandra ,  Erik Douglas ,  Amy Twite ,  Nicholas Toriello ,  Hiroaki Onoe

IPC分类号： C12N5/00 ,  A61K47/48

CPC分类号： C12N5/0006 ,  A61K47/6901

摘要： The present invention provides conjugates of DNA and cells by linking the DNA to a native functional group on the cell surface. The cells can be without cell walls or can have cell walls. The modified cells can be linked to a substrate surface and used in assay or bioreactors.

摘要翻译： 本发明通过将DNA与细胞表面上的天然官能团连接来提供DNA和细胞的结合物。 细胞可以没有细胞壁或可以具有细胞壁。 修饰的细胞可以连接到底物表面并用于测定或生物反应器中。

公开(公告)号：US20150072398A1

公开(公告)日：2015-03-12

申请号：US14464528

申请日：2014-08-20

申请人： The Regents of the University of California

发明人： Isaac S. Carrico ,  Brian L. Carlson ,  Peng Wu ,  Carolyn Bertozzi

IPC分类号： C12N9/10 ,  C07K14/35 ,  C07K14/565 ,  C07K14/00 ,  C07K16/00

CPC分类号： C07K16/00 ,  A61K38/1825 ,  A61K38/215 ,  A61K38/37 ,  A61K38/45 ,  A61K39/00 ,  C07K1/006 ,  C07K1/1072 ,  C07K1/13 ,  C07K14/00 ,  C07K14/35 ,  C07K14/565 ,  C07K16/46 ,  C07K2317/24 ,  C07K2317/40 ,  C07K2317/52 ,  C07K2317/622 ,  C12N9/13 ,  C12N9/96 ,  C12P21/00 ,  C12P21/005 ,  C12Y208/02

摘要： The invention features compositions and methods for site-specific modification of proteins by incorporation of an aldehyde tag. Enzymatic modification at a sulfatase motif of the aldehyde tag through action of a formylglycine generating enzyme (FGE) generates a formylglycine (FGly) residue. The aldehyde moiety of FGly residue can be exploited as a chemical handle for site-specific attachment of a moiety of interest to a polypeptide.

摘要翻译： 本发明的特征在于通过掺入醛标记蛋白的位点特异性修饰的组合物和方法。 通过甲酰甘氨酸生成酶（FGE）的作用，在醛标记的硫酸酯酶基序上进行酶促修饰产生甲酰甘氨酸（FGly）残基。 FGly残基的醛部分可以用作化学处理，用于将目标部分的位点特异性连接到多肽。

公开(公告)号：US11149296B2

公开(公告)日：2021-10-19

申请号：US15566594

申请日：2016-04-15

申请人： The Regents of the University of California

发明人： Peter Robinson ,  Cheng-Ting Tsai ,  Carolyn Bertozzi

IPC分类号： C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/686 ,  G01N33/543

摘要： Methods are provided for detecting antigen binding agents in samples. Aspects of the methods include detection of the aggregation of antigen binding agents with polynucleotide-bound antigens by sensitive proximity-based association of the antigen-bound polynucleotides. Aspects of the methods also include methods for the detection of such proximity-based association through nucleic acid amplification. In addition, compositions, e.g., reagents, kits, and devices, useful in practicing various embodiments of the methods are provided.

公开(公告)号：US09447390B2

公开(公告)日：2016-09-20

申请号：US14464528

申请日：2014-08-20

申请人： The Regents of the University of California

发明人： Isaac S. Carrico ,  Brian L. Carlson ,  Peng Wu ,  Carolyn Bertozzi

IPC分类号： C12N9/10 ,  C07K1/00 ,  C07K1/13 ,  C07K1/107 ,  C07K14/00 ,  C07K16/46 ,  C12P21/00 ,  C07K14/35 ,  C07K14/565 ,  C07K16/00 ,  C12N9/96

CPC分类号： C07K16/00 ,  A61K38/1825 ,  A61K38/215 ,  A61K38/37 ,  A61K38/45 ,  A61K39/00 ,  C07K1/006 ,  C07K1/1072 ,  C07K1/13 ,  C07K14/00 ,  C07K14/35 ,  C07K14/565 ,  C07K16/46 ,  C07K2317/24 ,  C07K2317/40 ,  C07K2317/52 ,  C07K2317/622 ,  C12N9/13 ,  C12N9/96 ,  C12P21/00 ,  C12P21/005 ,  C12Y208/02

摘要： The invention features compositions and methods for site-specific modification of proteins by incorporation of an aldehyde tag. Enzymatic modification at a sulfatase motif of the aldehyde tag through action of a formylglycine generating enzyme (FGE) generates a formylglycine (FGly) residue. The aldehyde moiety of FGly residue can be exploited as a chemical handle for site-specific attachment of a moiety of interest to a polypeptide.

公开(公告)号：US20150291699A1

公开(公告)日：2015-10-15

申请号：US14443149

申请日：2013-11-15

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： Carolyn Bertozzi ,  Paresh Agarwal ,  Ellen M. Sletten

IPC分类号： C07K16/32 ,  C07D495/04 ,  C07D405/12 ,  C07D498/04

CPC分类号： C07K16/32 ,  C07D209/12 ,  C07D311/92 ,  C07D405/12 ,  C07D495/04 ,  C07D498/04

摘要： Aldehyde- and ketone-functionalized proteins are promising new substrates for the development of chemically modified biotherapeutics and protein-based materials. Their reactive carbonyl groups are typically conjugated with a-effect nucleophiles, such as substituted hydrazines and alkoxyamines, to generate hydrazones and oximes, respectively. However, the resulting C═N linkages are susceptible to hydrolysis under physiologically relevant conditions, which limits their utility in biological systems. Here we introduce a Pictet-Spengler ligation that is based on the classic Pictet-Spengler reaction of aldehydes and tryptamine nucleophiles. The ligation exploits the bioorthogonal reaction of aldehydes and alkoxyamines to form an intermediate oxyiminium ion; this intermediate undergoes intramolecular C—C bond formation with an indole nucleophile to form an oxacarboline product that is hydrolytically stable. The reaction was utilized for site-specific chemical modification of glyoxal- and formylglycine-functionalized proteins, including an aldehyde-tagged variant of the therapeutic monoclonal antibody Herceptin. In conjunction with techniques for site-specific introduction of aldehydes into proteins, the Pictet-Spengler ligation offers a new means to generate stable bioconjugates for medical and materials applications.

摘要翻译： 醛和酮官能化的蛋白质是用于开发化学修饰的生物治疗剂和基于蛋白质的材料的有希望的新底物。 它们的反应性羰基通常与a-效应亲核试剂缀合，例如取代的肼和烷氧基胺，分别产生腙和肟。 然而，所得到的C = N键在生理相关条件下易于水解，这限制了它们在生物系统中的应用。 在这里，我们介绍一种基于醛和色胺亲核试剂的经典Pictet-Spengler反应的Pictet-Spengler连接。 连接利用醛和烷氧基胺的生物正交反应形成中间体氧化亚铵离子; 该中间体与吲哚亲核试剂进行分子内C-C键的形成，以形成水解稳定的恶唑啉产物。 该反应用于乙二醛和甲酰甘氨酸官能化蛋白质的位点特异性化学修饰，包括治疗性单克隆抗体赫赛汀的醛标记变体。 结合用于特定位点将醛引入蛋白质的技术，Pictet-Spengler连接为医疗和材料应用产生稳定的生物缀合物提供了新的手段。