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公开(公告)号：US20150218258A1
公开(公告)日：2015-08-06
申请号：US14426048
申请日：2013-09-19
CPC分类号： C07K16/18 , A61K47/68 , C07K16/2863 , C07K2317/40 , C07K2317/52 , C07K2317/732
摘要： The present disclosure provides modified Fc domains having one or more attachment moieties for attaching a heterologous functional moiety; and methods of generating the modified Fc domains. The present disclosure provides Fc conjugates; and methods of making the conjugates. The Fc conjugates are useful in various methods, which are also provided.
摘要翻译： 本公开提供具有一个或多个连接部分用于连接异源功能部分的修饰的Fc结构域; 以及生成修饰的Fc结构域的方法。 本公开提供Fc缀合物; 以及制备共轭物的方法。 Fc缀合物可用于各种方法，其也提供。
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2.发明申请公开(公告)号：US20160053344A1
公开(公告)日：2016-02-25
申请号：US14834074
申请日：2015-08-24
发明人： Matthew B. Francis , Troy Moore
CPC分类号： C22B3/24 , C22B59/00 , Y02P10/234
摘要： A sulfonamide based rare earth element ion separation media and method of synthesis and use are provided. A bed or column of sulfonamide resin for separations can be prepared by exposing a sulfonate resin to chlorosulfonic acid to form a sulfonyl chloride resin; exposing the sulfonyl chloride resin to aqueous ammonia to form a sulfonamide resin; and then packing the sulfonamide resin into a separation column. Mixtures of lanthanide and other rare earth ions with very similar atomic radii and characteristics can be separated by flowing a mixture of lanthanide ions through a bed of sulfonamide resin followed by a mobile phase of an organic acid such as lactic acid to elute the separated rare earth element ions separated by the sulfonamide resin. Collected fractions of eluate can also be recycled through the sulfonamide media.
摘要翻译： 提供磺酰胺类稀土元素离子分离介质及其合成方法。 用于分离的磺酰胺树脂床或柱可以通过将磺酸树脂暴露于氯磺酸形成磺酰氯树脂来制备; 将磺酰氯树脂暴露于氨水中形成磺酰胺树脂; 然后将磺酰胺树脂包装到分离塔中。 镧系元素和其他具有非常相似的原子半径和特性的稀土离子的混合物可以通过使镧系元素离子的混合物流过磺酰胺树脂的床，然后是诸如乳酸的有机酸的流动相来洗脱分离的稀土 元素离子被磺酰胺树脂分离。 收集的洗脱液馏分也可以通过磺酰胺介质再循环。
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公开(公告)号：US20180148472A1
公开(公告)日：2018-05-31
申请号：US15545459
申请日：2016-01-21
CPC分类号： C07K1/1077 , C07K1/13 , C08B37/0012 , C08B37/0015 , C08H1/00
摘要： Methods for preparing a protein conjugate having a defined number of conjugate groups are provided. The method includes: forming a mixture containing a macrocyclic matrix material and a plurality of proteins; eluting the proteins to obtain a first separated protein fraction and a second separated protein fraction, wherein substantially all of the proteins in the first separated protein fraction have the same number of handle moieties; contacting the handle moieties with a conversion reagent under conditions sufficient to convert the handle moieties in the first separated protein fraction to reactive moieties; and contacting the reactive moieties with a conjugation reagent under conditions sufficient to form a plurality of protein conjugates, wherein substantially all of the protein conjugates in the plurality have the same number of conjugate groups. Methods also include recovering enzymes and other proteins from mixtures for isolation and/or reuse of the enzymes and proteins.
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公开(公告)号：US09834597B2
公开(公告)日：2017-12-05
申请号：US14426048
申请日：2013-09-19
CPC分类号： C07K16/18 , A61K47/68 , C07K16/2863 , C07K2317/40 , C07K2317/52 , C07K2317/732
摘要： The present disclosure provides modified Fc domains having one or more attachment moieties for attaching a heterologous functional moiety; and methods of generating the modified Fc domains. The present disclosure provides Fc conjugates; and methods of making the conjugates. The Fc conjugates are useful in various methods, which are also provided.
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公开(公告)号：US09970075B2
公开(公告)日：2018-05-15
申请号：US14834074
申请日：2015-08-24
发明人： Matthew B. Francis , Troy Moore
CPC分类号： C22B3/24 , C22B59/00 , Y02P10/234
摘要： A sulfonamide based rare earth element ion separation media and method of synthesis and use are provided. A bed or column of sulfonamide resin for separations can be prepared by exposing a sulfonate resin to chlorosulfonic acid to form a sulfonyl chloride resin; exposing the sulfonyl chloride resin to aqueous ammonia to form a sulfonamide resin; and then packing the sulfonamide resin into a separation column. Mixtures of lanthanide and other rare earth ions with very similar atomic radii and characteristics can be separated by flowing a mixture of lanthanide ions through a bed of sulfonamide resin followed by a mobile phase of an organic acid such as lactic acid to elute the separated rare earth element ions separated by the sulfonamide resin. Collected fractions of eluate can also be recycled through the sulfonamide media.
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公开(公告)号：US20150343413A1
公开(公告)日：2015-12-03
申请号：US14729976
申请日：2015-06-03
IPC分类号： B01J19/12
CPC分类号： B01J19/123 , B01J2219/0881 , B01J2219/1203 , G03F7/008 , G03F7/0125 , G03F7/0755
摘要： The disclosure relates to methods of photopatterning molecules directly on a surface by photochemical means.
摘要翻译： 本公开涉及通过光化学方法直接在表面上对分子进行光图案化的方法。
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公开(公告)号：US20220153779A1
公开(公告)日：2022-05-19
申请号：US17440129
申请日：2020-03-19
发明人： Matthew B. Francis , Marco Jackson Lobba , Johnathan Charles Maza , Alan M. Marmelstein , Jennifer A. Doudna , Christof Fellmann , Casey S. Mogilevsky
摘要： The present disclosure provides a method for chemoselective modification of a target molecule. A subject method includes contacting a target molecule comprising a thiol moiety with a biomolecule comprising a reactive moiety, wherein the reactive moiety is generated by reaction of a biomolecule comprising a phenol moiety or a catechol with an enzyme capable of oxidizing the phenol or the catechol moiety. The contacting is carried out under conditions sufficient for conjugation of the target molecule to the biomolecule, thereby producing a modified target molecule. The present disclosure provides compositions comprising a subject target molecule comprising a thiol moiety, and a biomolecule comprising a phenol moiety or a catechol moiety. The present disclosure provides kits for carrying out a subject method. The present disclosure also provides modified target molecules and methods for using same.
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公开(公告)号：US10370407B2
公开(公告)日：2019-08-06
申请号：US15545459
申请日：2016-01-21
摘要： Methods for preparing a protein conjugate having a defined number of conjugate groups are provided. The method includes: forming a mixture containing a macrocyclic matrix material and a plurality of proteins; eluting the proteins to obtain a first separated protein fraction and a second separated protein fraction, wherein substantially all of the proteins in the first separated protein fraction have the same number of handle moieties; contacting the handle moieties with a conversion reagent under conditions sufficient to convert the handle moieties in the first separated protein fraction to reactive moieties; and contacting the reactive moieties with a conjugation reagent under conditions sufficient to form a plurality of protein conjugates, wherein substantially all of the protein conjugates in the plurality have the same number of conjugate groups. Methods also include recovering enzymes and other proteins from mixtures for isolation and/or reuse of the enzymes and proteins.
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公开(公告)号：US09969970B2
公开(公告)日：2018-05-15
申请号：US15057604
申请日：2016-03-01
发明人： Shih-Chia Hsiao , Matthew B. Francis , Carolyn Bertozzi , Richard Mathies , Ravi Chandra , Erik Douglas , Amy Twite , Nicholas Toriello , Hiroaki Onoe
CPC分类号： C12N5/0006 , A61K47/6901
摘要： The present invention provides conjugates of DNA and cells by linking the DNA to a native functional group on the cell surface. The cells can be without cell walls or can have cell walls. The modified cells can be linked to a substrate surface and used in assay or bioreactors.
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公开(公告)号：US09885071B2
公开(公告)日：2018-02-06
申请号：US14731381
申请日：2015-06-04
IPC分类号： A01N65/00 , C12P19/14 , C12P19/12 , C12N11/08 , C08F220/56 , C12P19/02 , C12N9/16 , C12N9/20 , C12N9/24 , C12N9/26 , C12N9/42 , C12N9/96
CPC分类号： C12P19/14 , C08F220/56 , C12N9/16 , C12N9/20 , C12N9/2402 , C12N9/2411 , C12N9/2437 , C12N9/2477 , C12N9/248 , C12N9/96 , C12N11/08 , C12P19/02 , C12P19/12 , C12P2203/00 , C08F220/60
摘要： Provided herein are methods for enhancing enzymatic activity using certain polymers that may be optionally attached to an enzyme. The polymers may be thermally-responsive polymers, including poly N-isopropylacrylamide or poly N-isopropylmethacrylamide. The polymer may also be a copolymer with at least two different monomer residues. The monomer residues may have a structure of formula (I): wherein R1, RA and RB are as described herein. Examples of such monomer residues may include N-isopropylacrylamide (NIPAm) or N-isopropylmethacrylamide (NIPMa). The polymer may include additional monomer residues, such as aminooxy-bearing methacrylamide monomer residues that can be modified to vary the lower critical solution temperature (LCST) of the polymer.
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