公开(公告)号：US10370407B2

公开(公告)日：2019-08-06

申请号：US15545459

申请日：2016-01-21

申请人： The Regents Of The University Of California

发明人： Richard L. Kwant ,  Matthew B. Francis ,  Christian B. Rosen ,  Meera Rao

IPC分类号： C07K1/107 ,  C07K1/13 ,  C08B37/16 ,  C08H1/00

摘要： Methods for preparing a protein conjugate having a defined number of conjugate groups are provided. The method includes: forming a mixture containing a macrocyclic matrix material and a plurality of proteins; eluting the proteins to obtain a first separated protein fraction and a second separated protein fraction, wherein substantially all of the proteins in the first separated protein fraction have the same number of handle moieties; contacting the handle moieties with a conversion reagent under conditions sufficient to convert the handle moieties in the first separated protein fraction to reactive moieties; and contacting the reactive moieties with a conjugation reagent under conditions sufficient to form a plurality of protein conjugates, wherein substantially all of the protein conjugates in the plurality have the same number of conjugate groups. Methods also include recovering enzymes and other proteins from mixtures for isolation and/or reuse of the enzymes and proteins.

公开(公告)号：US20180148472A1

公开(公告)日：2018-05-31

申请号：US15545459

申请日：2016-01-21

申请人： The Regents Of The University Of California

发明人： Richard L. Kwant ,  Matthew B. Francis ,  Christian B. Rosen ,  Meera Rao

IPC分类号： C07K1/107 ,  C08H1/00 ,  C07K1/13 ,  C08B37/16

CPC分类号： C07K1/1077 ,  C07K1/13 ,  C08B37/0012 ,  C08B37/0015 ,  C08H1/00

摘要： Methods for preparing a protein conjugate having a defined number of conjugate groups are provided. The method includes: forming a mixture containing a macrocyclic matrix material and a plurality of proteins; eluting the proteins to obtain a first separated protein fraction and a second separated protein fraction, wherein substantially all of the proteins in the first separated protein fraction have the same number of handle moieties; contacting the handle moieties with a conversion reagent under conditions sufficient to convert the handle moieties in the first separated protein fraction to reactive moieties; and contacting the reactive moieties with a conjugation reagent under conditions sufficient to form a plurality of protein conjugates, wherein substantially all of the protein conjugates in the plurality have the same number of conjugate groups. Methods also include recovering enzymes and other proteins from mixtures for isolation and/or reuse of the enzymes and proteins.