公开(公告)号：US20030070677A1

公开(公告)日：2003-04-17

申请号：US09911055

申请日：2001-07-23

申请人： The Regents of the University of Michigan

发明人： Kalyan Handique ,  Mark A. Burns

IPC分类号： A61M015/00

CPC分类号： B01F13/0071 ,  B01F5/0085 ,  B01F5/0403 ,  B01F11/0071 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00873 ,  B01J2219/00889 ,  B01J2219/00952 ,  B01L3/5027 ,  B01L2200/0605 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2400/0415 ,  B01L2400/0442 ,  B01L2400/0487 ,  B01L2400/0688 ,  B01L2400/0694 ,  B82Y30/00 ,  F16K99/0001 ,  F16K99/0017 ,  F16K99/0044 ,  F16K2099/0074 ,  F16K2099/008 ,  F16K2099/0084 ,  Y10T137/0318

摘要： The movement and mixing of microdroplets through microchannels is described employing microscale devices, comprising microdroplet transport channels, reaction regions, electrophoresis modules, and radiation detectors. Microdroplets are metered into defined volumes and are subsequently incorporated into a variety of biological assays. Electronic components are fabricated on the same substrate material, allowing sensors and controlling circuitry to be incorporated in the same device.

摘要翻译： 使用包括微滴输送通道，反应区域，电泳模块和辐射检测器的微量装置描述了通过微通道的微滴的运动和混合。 将微滴定量成定义的体积，随后并入多种生物测定中。 电子元件制造在相同的基板材料上，允许将传感器和控制电路并入同一设备。

公开(公告)号：US20040219732A1

公开(公告)日：2004-11-04

申请号：US10696889

申请日：2003-10-30

申请人： The Regents of the University of Michigan

发明人： Mark A. Burns ,  Rohit Pal

IPC分类号： H01L021/8238

CPC分类号： F16K99/0017 ,  B01F13/0059 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00788 ,  B01J2219/00828 ,  B01J2219/00873 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01L3/502707 ,  B01L3/502738 ,  B01L3/502784 ,  B01L2200/12 ,  B01L2400/0487 ,  B01L2400/0677 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0019 ,  F16K99/0036 ,  F16K99/0044 ,  F16K2099/0074 ,  F16K2099/0076 ,  F16K2099/0084 ,  Y10T137/2196 ,  Y10T137/4643

摘要： The movement and mixing of microdroplets through microchannels is described employing silicon-based microscale devices, comprising microdroplet transport channels, reaction regions, electrophoresis modules, and radiation detectors. The discrete droplets are differentially heated and propelled through etched channels. Electronic components are fabricated on the same substrate material, allowing sensors and controlling circuitry to be incorporated in the same device.

摘要翻译： 使用硅微量元件描述了通过微通道的微滴的运动和混合，包括微滴输送通道，反应区域，电泳模块和辐射探测器。 离散的液滴通过蚀刻通道进行差分加热和推进。 电子元件制造在相同的基板材料上，允许将传感器和控制电路并入同一设备。

公开(公告)号：US20030116437A1

公开(公告)日：2003-06-26

申请号：US10277348

申请日：2002-10-22

申请人： The Regents Of The University Of Michigan

发明人： Mark A. Burns ,  Sundaresh N. Brahmasandra ,  Victor M. Ugaz

IPC分类号： G01N027/453 ,  G01N027/27

CPC分类号： G01N27/44743 ,  G01N27/44704 ,  G01N27/44791

摘要： The present invention relates to a novel, small-scale, electrophoretic separation system based on photodefined polymers and electrode-defined sample injection. Diffusion and displacement coefficients may be modified by varying the gel concentration, the intensity of the incident UV radiation and the temperature at which the gel is run. The device is an major advance over current technology since it provides for a significant reduction in size of the micro-electrophoresis apparatus and a significant cost savings.

摘要翻译： 本发明涉及一种基于光定义聚合物和电极定义样品注入的新型小规模电泳分离系统。 可以通过改变凝胶浓度，入射的UV辐射的强度和凝胶运行的温度来改变扩散和置换系数。 该设备是超过现有技术的重大进步，因为它提供了显着减小微电泳装置的尺寸并显着降低了成本。

公开(公告)号：US20020168671A1

公开(公告)日：2002-11-14

申请号：US10113246

申请日：2002-04-01

申请人： The Regents of the University of Michigan

发明人： Mark A. Burns ,  David T. Burke ,  Brian N. Johnson ,  John D. DeNuzzio ,  Wayne F. Beyer JR.

IPC分类号： C12Q001/68 ,  C12M001/34 ,  C12M001/00

CPC分类号： B82Y30/00 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00853 ,  B01J2219/00871 ,  B01J2219/00873 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00912 ,  B01J2219/00952 ,  B01J2219/00961 ,  B01J2219/00986 ,  B01J2219/00995 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/00 ,  B01L7/52 ,  B01L7/54 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0861 ,  B01L2300/0887 ,  B01L2300/163 ,  B01L2300/165 ,  B01L2300/1805 ,  B01L2400/0406 ,  B01L2400/0442 ,  B01L2400/0448 ,  B01L2400/0677 ,  B01L2400/0688 ,  C12Q1/6844

摘要： Disclosed are methods and compositions for isothermal amplification of nucleic acids in a microfabricated substrate. Methods and compositions for the analysis of isothermally amplified nucleic acids in a microfabricated substrate are disclosed as well. The microfabricated substrates and isothermal amplification and detection methods provided are envisioned for use in various diagnostic methods, particularly those connected with diseases characterized by altered gene sequences or gene expression.

摘要翻译： 公开了用于在微加工基质中等温扩增核酸的方法和组合物。 还公开了用于分析微细加工底物中的等温扩增核酸的方法和组合物。 所提供的微细化底物和等温扩增和检测方法被设想用于各种诊断方法，特别是那些以改变的基因序列或基因表达为特征的疾病。

公开(公告)号：US20030213693A1

公开(公告)日：2003-11-20

申请号：US10342935

申请日：2003-01-15

申请人： The Regents Of The University Of Michigan

发明人： Ponnambalam Selvaganapathy ,  Mark A. Burns ,  David T. Burke ,  Carlos H. Mastrangelo

IPC分类号： G01N027/26 ,  G01N027/27

CPC分类号： G01N27/4473 ,  G01N27/44791

摘要： The present invention relates to microfabrication and utilization of microscale electrophoresis devices as well as the separation and detection of biomolecules in microscale electrophoresis devices. The device of the present invention utilizes novel fabrication and detection methods.

摘要翻译： 本发明涉及微细电泳装置的微细加工和利用以及微电泳装置中生物分子的分离和检测。 本发明的装置利用新的制造和检测方法。

公开(公告)号：US20020172969A1

公开(公告)日：2002-11-21

申请号：US10113238

申请日：2002-04-01

申请人： The Regents of the University of Michigan

发明人： Mark A. Burns ,  David T. Burke ,  Brian N. Johnson ,  John D. DeNuzzio ,  Wayne F. Beyer JR.

IPC分类号： C12Q001/68 ,  C12M001/34 ,  C12M001/00

CPC分类号： B82Y30/00 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00826 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00853 ,  B01J2219/00871 ,  B01J2219/00873 ,  B01J2219/00889 ,  B01J2219/00891 ,  B01J2219/00912 ,  B01J2219/00952 ,  B01J2219/00961 ,  B01J2219/00986 ,  B01J2219/00995 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/00 ,  B01L7/52 ,  B01L7/54 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0861 ,  B01L2300/0887 ,  B01L2300/163 ,  B01L2300/165 ,  B01L2300/1805 ,  B01L2400/0406 ,  B01L2400/0442 ,  B01L2400/0448 ,  B01L2400/0677 ,  B01L2400/0688 ,  C12Q1/6844

摘要： Disclosed are methods and compositions for isothermal amplification of nucleic acids in a microfabricated substrate. Methods and compositions for the analysis of isothermally amplified nucleic acids in a microfabricated substrate are disclosed as well. The microfabricated substrates and isothermal amplification and detection methods provided are envisioned for use in various diagnostic methods, particularly those connected with diseases characterized by altered gene sequences or gene expression.

公开(公告)号：US20010046703A1

公开(公告)日：2001-11-29

申请号：US09751493

申请日：2000-12-28

申请人： The Regents Of The University Of Michigan

发明人： Mark A. Burns ,  Carlos H. Mastrangelo ,  Timothy S. Sammarco ,  Francis P. Man ,  James R. Webster ,  Brian N. Johnson ,  Bradley Foerster ,  Darren Jones ,  Yakeitha Fields ,  Adam Kaiser ,  David T. Burke

IPC分类号： C12M001/00 ,  H05B011/00

CPC分类号： B01F13/0072 ,  B01F5/0085 ,  B01F5/0403 ,  B01F13/0071 ,  B01F13/0084 ,  B01F13/0093 ,  B01F15/0232 ,  F15B13/0807 ,  F15C5/00 ,  F16K99/0001 ,  F16K99/0017 ,  F16K99/0019 ,  F16K99/0028 ,  F16K99/0036 ,  F16K99/0044 ,  F16K2099/0074 ,  F16K2099/008 ,  F16K2099/0084 ,  G05D23/1917 ,  Y10T436/143333

摘要： The movement and mixing of microdroplets through microchannels is described employing silicon-based microscale devices, comprising microdroplet transport channels, reaction regions, electrophoresis modules, and radiation detectors. The discrete droplets are differentially heated and propelled through etched channels. Electronic components are fabricated on the same substrate material, allowing sensors and controlling circuitry to be incorporated in the same device.