公开(公告)号：US10308691B2

公开(公告)日：2019-06-04

申请号：US15535466

申请日：2015-12-14

Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED

Inventor： Yanbin Liu ,  Chong Mei Koh ,  Lianghui Ji

IPC: C12N15/80 ,  C07K14/37 ,  C12P23/00 ,  C12N9/02 ,  C12N15/113 ,  C12P21/02

Abstract: The present invention relates to the field of fungal biotechnology, more particularly to genetic engineering methods for the production of carotenoids in fungal hosts selected from Rhodospordium and Rhodotorula genera.

公开(公告)号：US11254943B2

公开(公告)日：2022-02-22

申请号：US16070648

申请日：2017-02-02

Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED

Inventor： Lianghui Ji ,  Yanbin Liu ,  Chong Mei John Koh ,  Sihui Amy Yap

IPC: C12N15/81 ,  C12P1/02

Abstract: The present invention relates to the field of molecular biology and more particularly to promoters useful for metabolic engineering in yeast or fungi for the production of biobased chemicals with broad applications. Intron-containing promoters with strong activity during oil-accumulation stages are particularly useful for genetic engineering in yeast and fungi, particularly Rhodosporidium or Rhodotorula genera. Such promoter are capable of driving strong expression of RNA or proteins in species of the Rhodosporidium or Rhodotorula genera.

公开(公告)号：US10081821B2

公开(公告)日：2018-09-25

申请号：US15326077

申请日：2015-08-21

Applicant: Temasek Life Sciences Laboratory Limited

Inventor： Yanbin Liu ,  Chong Mei Koh ,  Lianghui Ji

IPC: C12N1/14 ,  C12N1/15 ,  C12N9/02 ,  C12P7/64 ,  C12N15/52 ,  C12N15/81 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90 ,  C12N9/00

Abstract: The present invention relates to the field of fungal biotechnology, more particularly to genetic engineering methods for the production of polyunsaturated fatty acids (PUFA) in fungal hosts selected from Rhodosporidium and Rhodotorula genera. The present invention further relates to a modified fungal host cell having reduced native aldehyde dehydrogenase (ALD 1) enzyme activity, and methods for producing omega-3 and omega-6 fatty acids and triacylglycerides, by growing said fungal host cell under suitable conditions.

公开(公告)号：US11034981B2

公开(公告)日：2021-06-15

申请号：US16484322

申请日：2018-01-29

Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED

Inventor： Lianghui Ji ,  Yanbin Liu ,  Chong Mei John Koh ,  Sihui Amy Yap ,  Si Te Ngoh

IPC: C12P7/04

Abstract: The present invention relates to the field of fungal production fatty alcohols. More specifically, the present invention relates to genetically modified host cells, nucleic acid constructs and culture medium for the production of fatty alcohols in Rhodosporidium.

公开(公告)号：US10081812B2

公开(公告)日：2018-09-25

申请号：US15563176

申请日：2016-03-31

Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITED

Inventor： Lianghui Ji ,  Yanbin Liu ,  Chong Mei John Koh

IPC: C12N15/81 ,  C12N15/80 ,  C12N1/16

CPC classification number: C12N15/815 ,  C12N1/16 ,  C12N15/80 ,  C12N15/81 ,  C12N2830/36 ,  C12N2840/002

Abstract: The present invention relates to the field of fungal biotechnology, more particularly to a strong inducible gene expression system in fungal species, such as a species of the Rhodosporidium genus or the Rhodotorula genus.