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公开(公告)号：US10308691B2
公开(公告)日：2019-06-04
申请号：US15535466
申请日：2015-12-14
发明人： Yanbin Liu , Chong Mei Koh , Lianghui Ji
摘要： The present invention relates to the field of fungal biotechnology, more particularly to genetic engineering methods for the production of carotenoids in fungal hosts selected from Rhodospordium and Rhodotorula genera.
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公开(公告)号：US11254943B2
公开(公告)日：2022-02-22
申请号：US16070648
申请日：2017-02-02
发明人： Lianghui Ji , Yanbin Liu , Chong Mei John Koh , Sihui Amy Yap
摘要： The present invention relates to the field of molecular biology and more particularly to promoters useful for metabolic engineering in yeast or fungi for the production of biobased chemicals with broad applications. Intron-containing promoters with strong activity during oil-accumulation stages are particularly useful for genetic engineering in yeast and fungi, particularly Rhodosporidium or Rhodotorula genera. Such promoter are capable of driving strong expression of RNA or proteins in species of the Rhodosporidium or Rhodotorula genera.
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公开(公告)号：US10081821B2
公开(公告)日：2018-09-25
申请号：US15326077
申请日：2015-08-21
发明人： Yanbin Liu , Chong Mei Koh , Lianghui Ji
IPC分类号： C12N1/14 , C12N1/15 , C12N9/02 , C12P7/64 , C12N15/52 , C12N15/81 , C12N9/04 , C12N9/10 , C12N9/88 , C12N9/90 , C12N9/00
摘要： The present invention relates to the field of fungal biotechnology, more particularly to genetic engineering methods for the production of polyunsaturated fatty acids (PUFA) in fungal hosts selected from Rhodosporidium and Rhodotorula genera. The present invention further relates to a modified fungal host cell having reduced native aldehyde dehydrogenase (ALD 1) enzyme activity, and methods for producing omega-3 and omega-6 fatty acids and triacylglycerides, by growing said fungal host cell under suitable conditions.
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公开(公告)号：US11034981B2
公开(公告)日：2021-06-15
申请号：US16484322
申请日：2018-01-29
发明人： Lianghui Ji , Yanbin Liu , Chong Mei John Koh , Sihui Amy Yap , Si Te Ngoh
IPC分类号： C12P7/04
摘要： The present invention relates to the field of fungal production fatty alcohols. More specifically, the present invention relates to genetically modified host cells, nucleic acid constructs and culture medium for the production of fatty alcohols in Rhodosporidium.
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公开(公告)号：US10081812B2
公开(公告)日：2018-09-25
申请号：US15563176
申请日：2016-03-31
发明人： Lianghui Ji , Yanbin Liu , Chong Mei John Koh
CPC分类号： C12N15/815 , C12N1/16 , C12N15/80 , C12N15/81 , C12N2830/36 , C12N2840/002
摘要： The present invention relates to the field of fungal biotechnology, more particularly to a strong inducible gene expression system in fungal species, such as a species of the Rhodosporidium genus or the Rhodotorula genus.
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