公开(公告)号：US20130175169A1

公开(公告)日：2013-07-11

申请号：US13784168

申请日：2013-03-04

Applicant: TECAN TRADING AG

Inventor： Marc N. Feiglin ,  Michael Benjamin Franklin ,  Timothy Francis Lee ,  Joseph Mamone

IPC: G01N27/447

CPC classification number: B01L3/502792 ,  B01F13/0071 ,  B01F13/0076 ,  B01F2215/0037 ,  B01L3/0268 ,  B01L3/502715 ,  B01L3/527 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/1822 ,  B01L2300/1883 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0427 ,  B01L2400/0475 ,  C12Q1/686 ,  G01N27/447 ,  G01N27/44756 ,  G01N27/453 ,  C12Q2563/143 ,  C12Q2563/149 ,  C12Q2563/159

Abstract: Liquid droplet manipulation instrument has electrode array for inducing movement of a droplet by electrowetting, substrate supporting the array and control with electrode selector connected to a voltage control. The selector selects each electrode and provides each with a controlled voltage. The control includes central processing unit for providing the selected electrode with an individual voltage pulse which is a drive voltage or a ground voltage or a stop voltage. The control defines a path for movement of a liquid portion of a larger volume that covers more than one electrode by the simultaneous selection of a group of two or more subsequent drive electrodes and to provide each selected drive electrode with a drive voltage pulse along the path. The control simultaneously provides a group of two or more electrodes adjacent to or identical with the pulsed drive electrodes, with a ground or stop voltage pulse.

Abstract translation: 液滴操作仪具有电极阵列，用于通过电润湿引起液滴的移动，支撑阵列的基板和用电压控制器连接的电极选择器进行控制。 选择器选择每个电极，并为每个电极提供受控电压。 该控制器包括用于向选定电极提供作为驱动电压或接地电压或停止电压的单独电压脉冲的中央处理单元。 控制器通过同时选择一组两个或更多个后续驱动电极来定义用于移动覆盖多于一个电极的较大体积的液体部分的路径，并且为每个选定的驱动电极提供沿路径的驱动电压脉冲 。 控制器同时提供与脉冲驱动电极相邻或相同的一组两个或更多个电极，具有接地或停止电压脉冲。

公开(公告)号：US08936708B2

公开(公告)日：2015-01-20

申请号：US13784168

申请日：2013-03-04

Applicant: Tecan Trading AG

Inventor： Marc N. Feiglin ,  Michael Benjamin Franklin ,  Timothy Francis Lee ,  Joseph Mamone

IPC: G01N27/447 ,  G01N27/453 ,  B01L3/00 ,  B01L7/00 ,  B01F13/00 ,  C12Q1/68 ,  B01L3/02

CPC classification number: B01L3/502792 ,  B01F13/0071 ,  B01F13/0076 ,  B01F2215/0037 ,  B01L3/0268 ,  B01L3/502715 ,  B01L3/527 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/1822 ,  B01L2300/1883 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0427 ,  B01L2400/0475 ,  C12Q1/686 ,  G01N27/447 ,  G01N27/44756 ,  G01N27/453 ,  C12Q2563/143 ,  C12Q2563/149 ,  C12Q2563/159

Abstract: Liquid droplet manipulation instrument has electrode array for inducing movement of a droplet by electrowetting, substrate supporting the array and control with electrode selector connected to a voltage control. The selector selects each electrode and provides each with a controlled voltage. The control includes central processing unit for providing the selected electrode with an individual voltage pulse which is a drive voltage or a ground voltage or a stop voltage. The control defines a path for movement of a liquid portion of a larger volume that covers more than one electrode by the simultaneous selection of a group of two or more subsequent drive electrodes and to provide each selected drive electrode with a drive voltage pulse along the path. The control simultaneously provides a group of two or more electrodes adjacent to or identical with the pulsed drive electrodes, with a ground or stop voltage pulse.

Abstract translation: 液滴操作仪具有电极阵列，用于通过电润湿引起液滴的移动，支撑阵列的基板和用电压控制器连接的电极选择器进行控制。 选择器选择每个电极，并为每个电极提供受控电压。 该控制器包括用于向所选择的电极提供作为驱动电压或接地电压或停止电压的单独电压脉冲的中央处理单元。 控制器通过同时选择一组两个或更多个后续驱动电极来定义用于移动覆盖多于一个电极的较大体积的液体部分的路径，并且为每个选定的驱动电极提供沿路径的驱动电压脉冲 。 控制器同时提供与脉冲驱动电极相邻或相同的一组两个或更多个电极，具有接地或停止电压脉冲。

公开(公告)号：US09783842B2

公开(公告)日：2017-10-10

申请号：US14372796

申请日：2013-01-18

Applicant: TECAN TRADING AG

Inventor： Joseph Mamone ,  Marc N. Feiglin ,  Howard Gamper

IPC: C12Q1/68

CPC classification number: C12Q1/6813 ,  C12Q1/6827 ,  C12Q2525/151 ,  C12Q2525/186

Abstract: In a method of deducing the number of repeat units in a selected short tandem repeat (STR) in a genomic sample, at least a single stranded target DNA generated from a genomic sample comprising a selected STR, an STR probe (P1,P1′), a reference probe (P2), and two blockers (B1,B2) are provided, and at least three differential hybridization experiments are carried out, based on which the number of STR probe oligonucleotides (P1,P1′) bound per target DNA strand in each differential hybridization experiment is determined. The method further comprises the step of comparing these numbers of STR probe oligonucleotides (P1,P1′) bound per target DNA strand in the differential hybridization experiments for deducing the number of repeat units in the selected STR on the single stranded target DNA strand. Also disclosed are kits for carrying out STR genotyping by differential hybridization.

公开(公告)号：US20150010908A1

公开(公告)日：2015-01-08

申请号：US14372796

申请日：2013-01-18

Applicant: TECAN TRADING AG

Inventor： Joseph Mamone ,  Marc N. Feiglin ,  Howard Gamper

IPC: C12Q1/68

CPC classification number: C12Q1/6813 ,  C12Q1/6827 ,  C12Q2525/151 ,  C12Q2525/186

Abstract: In a method of deducing the number of repeat units in a selected short tandem repeat (STR) in a genomic sample, at least a single stranded target DNA generated from a genomic sample comprising a selected STR, an STR probe (P1,P1′), a reference probe (P2), and two blockers (B1,B2) are provided, and at least three differential hybridization experiments are carried out, based on which the number of STR probe oligonucleotides (P1,P1′) bound per target DNA strand in each differential hybridization experiment is determined. The method further comprises the step of comparing these numbers of STR probe oligonucleotides (P1,P1′) bound per target DNA strand in the differential hybridization experiments for deducing the number of repeat units in the selected STR on the single stranded target DNA strand. Also disclosed are kits for carrying out STR genotyping by differential hybridization.

Abstract translation: 在基因组样品中推定所选短串联重复序列（STR）中的重复单元数的方法中，至少由包含选定的STR，STR探针（P1，P1'）的基因组样品产生的单链靶DNA， ，提供参考探针（P2）和两个阻断剂（B1，B2），并进行至少三个差异杂交实验，基于此，每个靶DNA链结合的STR探针寡核苷酸（P1，P1'）的数量 在每个差异杂交实验中确定。 该方法还包括在差异杂交实验中比较在每个靶DNA链上结合的STR探针寡核苷酸（P1，P1'）数目，以推断单链靶DNA DNA链上所选STR中重复单元数目的步骤。 还公开了用于通过差异杂交进行STR基因分型的试剂盒。