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公开(公告)号：US20190048387A1
公开(公告)日：2019-02-14
申请号：US16165074
申请日：2018-10-19
发明人： Shiro Kitano , Kei Tsukamoto , Shinji Irie
IPC分类号： C12Q1/02
摘要： A method of assessing a possibility of cancerization including culturing a cell structure including normal cells and having a vascular network structure in a presence of a biological specimen from a subject, and assessing a possibility of cancerization in the subject based on a state of vessels in the cell structure after the culturing. The biological specimen is a body fluid specimen from the subject, a cell extract of cells from the subject, or a culture supernatant of cells from the subject, and the possibility of cancerization is assessed as high in the subject when a number of cells forming the vessels in the cell structure is larger than a number of cells cultured in an absence of the body fluid specimen, or when the vascular network structure in the cell structure extends.
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公开(公告)号：US11179495B2
公开(公告)日：2021-11-23
申请号：US16480114
申请日：2018-01-31
发明人： Michiya Matsusaki , Shinji Irie , Shiro Kitano
摘要： Disclosed is a three-dimensional tissue comprising cells and collagen including endogenous collagen, wherein at least a portion of the cells is adhered to the collagen, and the content of the collagen is from 10 wt % to 90 wt % based on the three-dimensional tissue.
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3.发明授权公开(公告)号：US11746382B2
公开(公告)日：2023-09-05
申请号：US16506489
申请日：2019-07-09
发明人： Shiro Kitano , Takeshi Yamada , Goro Takahashi , Takuma Iwai , Koki Takeda
IPC分类号： C07H21/04 , C12Q1/68 , C12Q1/6886 , G16B40/10 , C12Q1/6827 , C12Q1/6851 , C12Q1/686
CPC分类号： C12Q1/6886 , C12Q1/686 , C12Q1/6827 , C12Q1/6851 , G16B40/10
摘要： A method of detecting somatic mutations of tumor marker genes includes recovering a peripheral blood mononuclear cell layer from a blood sample collected from a subject and examining whether or not a nucleic acid derived from tumor marker genes having somatic mutations is detected from DNA included in the peripheral blood mononuclear cell layer.
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公开(公告)号：US11492652B2
公开(公告)日：2022-11-08
申请号：US16165074
申请日：2018-10-19
发明人： Shiro Kitano , Kei Tsukamoto , Shinji Irie
IPC分类号： C12Q1/02
摘要： A method of assessing a possibility of cancerization including culturing a cell structure including normal cells and having a vascular network structure in a presence of a biological specimen from a subject, and assessing a possibility of cancerization in the subject based on a state of vessels in the cell structure after the culturing. The biological specimen is a body fluid specimen from the subject, a cell extract of cells from the subject, or a culture supernatant of cells from the subject, and the possibility of cancerization is assessed as high in the subject when a number of cells forming the vessels in the cell structure is larger than a number of cells cultured in an absence of the body fluid specimen, or when the vascular network structure in the cell structure extends.
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5.发明申请公开(公告)号：US20180135138A1
公开(公告)日：2018-05-17
申请号：US15870190
申请日：2018-01-12
发明人： Shiro Kitano , Takeshi Yamada , Takuma Iwai
IPC分类号： C12Q1/6886
CPC分类号： C12Q1/6886 , A61K31/282 , A61K31/513 , A61K31/519 , A61K31/7068 , A61K39/3955 , C07K16/22 , C07K16/2863 , C07K2317/24 , C07K2317/76 , C12Q2600/106 , C12Q2600/156 , G01N2800/52 , A61K2300/00
摘要： A method for evaluating a health condition, includes: measuring a test amount of cell free DNA per unit amount of a body fluid sample collected from a subject after tumor resection; and comparing the measured test amount of cell free DNA with a reference value so as to evaluate a health condition of the subject. The reference value is one of a predetermined threshold value; an amount of cell free DNA per unit amount of a body fluid sample collected from the subject before tumor resection; an amount of cell free DNA per unit amount of a body fluid sample collected from the subject between 1 week and 3 months after tumor resection; and an amount of cell free DNA per unit amount of a body fluid sample collected from the subject by the time of collecting the body fluid sample for measuring the test amount of cell free DNA.
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公开(公告)号：US11479764B2
公开(公告)日：2022-10-25
申请号：US16951456
申请日：2020-11-18
发明人： Hiroki Nagase , Kei Tsukamoto , Shiro Kitano
摘要： A method of separating a target double-stranded nucleic acid molecule from a sample including the target double-stranded nucleic acid molecule and a non-target double-stranded nucleic acid molecule, including (1) mixing the sample, a pyrrole-imidazole-containing polyamide (first PI polyamide) modified with a first linker molecule and capable of specifically binding to a sequence of the target double-stranded nucleic acid molecule, and a carrier a modified with a first ligand capable of specifically binding and/or adsorbing to the first linker molecule such that a mixed solution is produced, (2) forming a complex A by binding the carrier a to the first PI polyamide with which the target double-stranded nucleic acid molecule is bound in the mixed solution, and (3) separating the complex A from the mixed solution.
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公开(公告)号：US20180355308A1
公开(公告)日：2018-12-13
申请号：US16105625
申请日：2018-08-20
发明人： Michiya Matsusaki , Shinji Irie , Shiro Kitano
CPC分类号： C12N5/0062 , C12N5/0656 , C12N5/0693 , C12N2501/91 , C12N2501/998 , C12N2513/00
摘要： A method of producing a three-dimensional cell tissue, including: a step A of mixing cells with a cationic substance and an extracellular matrix component to obtain a mixture; a step B of gathering the cells from the obtained mixture to form a cell aggregate on a substrate; and a step C of culturing the cells to obtain a three-dimensional cell tissue.
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公开(公告)号：US11781115B2
公开(公告)日：2023-10-10
申请号：US16782579
申请日：2020-02-05
IPC分类号： C12N5/09
CPC分类号： C12N5/0693 , C12N2502/11 , C12N2502/1323 , C12N2502/28 , C12N2509/10 , C12N2513/00 , C12N2533/30 , C12N2533/90
摘要： A primary culture method in which cells contained in a tissue collected from a living body are primary cultured in vitro, in which the cells in the tissue collected from the living body are seeded and cultured on a top surface of a cell structure containing cells constituting a stroma and composed of a single layer or two or more cell layers laminated in the thickness direction.
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公开(公告)号：US11249070B2
公开(公告)日：2022-02-15
申请号：US16164989
申请日：2018-10-19
发明人： Shiro Kitano , Kei Tsukamoto , Shinji Irie
摘要： A method of assessing an anti-cancer drug including culturing a cell structure including cancer cells and stromal cells in a presence of at least one anti-cancer drug, and assessing an anti-cancer effect of the at least one anti-cancer drug based on a number of viable cancer cells in the cell structure after the culturing.
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公开(公告)号：US11221326B2
公开(公告)日：2022-01-11
申请号：US16164914
申请日：2018-10-19
发明人： Shiro Kitano , Kei Tsukamoto , Shinji Irie
摘要： A method of producing drug resistant cells including contacting exosomes from a living organism exhibiting drug resistance with cells exhibiting no drug resistance such that drug resistant cells are formed, and culturing the drug resistant cells.
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