公开(公告)号：US09952421B2

公开(公告)日：2018-04-24

申请号：US15549832

申请日：2016-02-04

Applicant: Stichting Vu

Inventor： Andreas Sebastian Biebricher ,  Andrea Candelli ,  Iddo Heller ,  Niels Laurens ,  Erwin Johannes Gerard Peterman ,  Gijs Jan Lodewijk Wuite

IPC: G02B21/32 ,  G02B27/14 ,  G02B26/08 ,  G02B27/28 ,  G02B21/16 ,  G02B21/18 ,  G02B21/36 ,  G01N21/64 ,  B01L3/00 ,  G02B27/10

CPC classification number: G02B21/32 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2300/0654 ,  B01L2400/0454 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2021/6439 ,  G02B21/16 ,  G02B21/18 ,  G02B21/361 ,  G02B26/0816 ,  G02B27/106 ,  G02B27/141 ,  G02B27/283

Abstract: The present disclosure relates to an apparatus (100) and method for controlling a plurality of simultaneously active optical traps (OT1,OT2,OT3). In one method, trapping beams (TB1,TB2,TB3) are provided and redirected for individually controlling a respective position (X,Y) of optical traps (OT1,OT2,OT3) formed by focusing of the redirected trapping beams in a sample volume (SV). Light (L11,L20) from the sample volume (SV) corresponding to the optical traps is received. A path of a detector beam (AB) is overlapped with one of the trapping beams (TB3), wherein the detector beam has a distinct wavelength (λA) from that of the overlapping trapping beam (TB3). In one channel, the light from the sample volume is filtered according to wavelength, and only the filtered light having the wavelength (λA) of the detector beam (AB) is measured.

公开(公告)号：US20180024342A1

公开(公告)日：2018-01-25

申请号：US15549832

申请日：2016-02-04

Applicant: Stichting Vu

Inventor： Andreas Sebastian Biebricher ,  Andrea Candelli ,  Iddo Heller ,  Niels Laurens ,  Erwin Johannes Gerard Peterman ,  Gijs Jan Lodewijk Wuite

IPC: G02B21/32 ,  G02B27/14 ,  G02B26/08 ,  B01L3/00 ,  G02B21/16 ,  G02B21/18 ,  G02B21/36 ,  G01N21/64 ,  G02B27/10 ,  G02B27/28

CPC classification number: G02B21/32 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2300/0654 ,  B01L2400/0454 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2021/6439 ,  G02B21/16 ,  G02B21/18 ,  G02B21/361 ,  G02B26/0816 ,  G02B27/106 ,  G02B27/141 ,  G02B27/283

Abstract: The present disclosure relates to an apparatus (100) and method for controlling a plurality of simultaneously active optical traps (OT1, OT2, OT3). In one method, trapping beams (TB1, TB2, TB3) are provided and redirected for individually controlling a respective position (X,Y) of optical traps (OT1, OT2, OT3) formed by focusing of the redirected trapping beams in a sample volume (SV). Light (L11,L20) from the sample volume (SV) corresponding to the optical traps is received. A path of a detector beam (AB) is overlapped with one of the trapping beams (TB3), wherein the detector beam has a distinct wavelength (λA) from that of the overlapping trapping beam (TB3). In one channel, the light from the sample volume is filtered according to wavelength, and only the filtered light having the wavelength (λA) of the detector beam (AB) is measured.

公开(公告)号：US20230366801A1

公开(公告)日：2023-11-16

申请号：US18030034

申请日：2021-10-05

Applicant: Stichting VU ,  LUMICKS CA HOLDING B.V.

Inventor： Andreas Sebastian Biebricher ,  Vadim Bogatyr ,  Gijs Jan Lodewijk Wuite ,  Erwin Johannes Gerard Peterman ,  Iddo Heller ,  Rogier Martijn Reijmers

IPC: G01N15/14 ,  G06T7/60 ,  G06T7/20 ,  G06T7/70 ,  G06V20/69

CPC classification number: G01N15/1404 ,  G01N15/1434 ,  G06T7/60 ,  G06T7/20 ,  G06T7/70 ,  G06V20/698 ,  G01N2015/149 ,  G01N2015/1493 ,  G01N2015/1075

Abstract: A method comprises receiving images representing manipulating cellular bodies that includes exerting force pulses to the bodies on a wall surface; analyzing the images to determine the size of the bodies and tracking locations during and after each of force pulses, the tracking locations defining first trajectories of the bodies moving away from the wall surface and trajectories of the bodies moving towards the wall surface; determining densities of the bodies using the second trajectories and a sedimentation model of the bodies moving towards the wall surface and determining body velocities based on the first trajectories and a velocity model of the bodies moving away from the wall surface; and, determining a contrast factor for each body based on the sizes and the densities, the force applied to the bodies and the body velocities and determining a compressibility for each of the bodies based on the determined contrast factors.