公开(公告)号：US10184930B2

公开(公告)日：2019-01-22

申请号：US15039825

申请日：2014-11-26

Applicant: STC.UNM

Inventor： Steven R. J. Brueck ,  Jeremy Scott Edwards ,  Alexander Neumann ,  Yuliya Kuznetsova ,  Edgar A. Mendoza

IPC: G01N21/00 ,  G01N33/487 ,  C12Q1/6869 ,  B01L3/00 ,  C23C16/34 ,  C23C16/455 ,  C23C16/50 ,  G01N21/65

Abstract: Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.

公开(公告)号：US20190227050A1

公开(公告)日：2019-07-25

申请号：US16215139

申请日：2018-12-10

Applicant: STC.UNM

Inventor： Steven R.J. Brueck ,  Jeremy Scott Edwards ,  Alexander Neumann ,  Yuliya Kuznetsova ,  Edgar A. Mendoza

IPC: G01N33/487 ,  C23C16/50 ,  C23C16/455 ,  C23C16/34 ,  B01L3/00 ,  C12Q1/6869 ,  G01N21/65

Abstract: Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.

公开(公告)号：US20160377590A1

公开(公告)日：2016-12-29

申请号：US15039825

申请日：2014-11-26

Applicant: STC.UNM

Inventor： Steven R.J. Brueck ,  Jeremy Scott Edwards ,  Alexander Neumann ,  Yuliya Kuznetsova ,  Edgar A. Mendoza

IPC: G01N33/487 ,  B01L3/00 ,  C23C16/50 ,  C23C16/34 ,  C23C16/455 ,  C12Q1/68 ,  G01N21/65

CPC classification number: G01N33/48721 ,  B01L3/502707 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2300/046 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/0896 ,  B01L2300/16 ,  B01L2300/168 ,  B01L2400/0421 ,  C12Q1/6869 ,  C23C16/345 ,  C23C16/45525 ,  C23C16/50 ,  G01N21/65 ,  G01N2021/653 ,  G01N2201/061 ,  C12Q2563/155 ,  C12Q2565/631

Abstract: Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.

Abstract translation: 长时间读取，无标记，光学纳米孔长链分子测序的方法和设备。 通常，本公开描述了基于纳米通道的整合以提供具有广泛间隔（>波长），〜1nm孔径“曲折”纳米孔的单个长链分子的新型测序技术，其缓慢移位足以提供大量平行的， 使用光学技术的单基准分辨率。 使用简单的胶体纳米颗粒的新颖的定向自组装纳米加工方案用于在展开长链分子的纳米通道上形成纳米孔阵列。 在纳米颗粒阵列的表面，工程等离子体/极化结构中的强定域电磁场允许使用光学技术的单碱基分辨率。