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    Methods of nucleic acid target capture
    1.
    发明申请
    Methods of nucleic acid target capture 有权
    核酸靶捕获方法

    公开(公告)号：US20060252085A1

    公开(公告)日：2006-11-09

    申请号：US11429304

    申请日：2006-05-05

    申请人： Reinhold Pollner ,  Michael Becker ,  Mehrdad Majlessi

    发明人： Reinhold Pollner ,  Michael Becker ,  Mehrdad Majlessi

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6813 ,  C12Q1/6834 ,  Y10T436/143333 ,  C12Q2537/125 ,  C12Q2523/113 ,  C12Q2565/519 ,  C12Q2527/101

    摘要： Methods for efficiently capturing a target nucleic acid from a sample by using a mixture that contains a capture probe specific for the target nucleic acid, the target nucleic acid, and a denaturant chemical, which mixture is incubated at elevated temperature for a short time, are disclosed. Compositions that include a capture probe that specifically binds to a target nucleic acid and a denaturant chemical, which when mixed with the target nucleic acid and incubated at elevated temperature for a short time, promote efficient hybridization of the capture probe and target nucleic acid are disclosed.

    摘要翻译： 通过使用含有目标核酸特异性的捕获探针，目标核酸和变性剂化合物的混合物在高温下短时间培养来有效地从样品中捕获靶核酸的方法是： 披露 公开了包含与目标核酸和变性剂化学物质特异性结合的捕获探针的组合物，当与目标核酸混合并在高温下温育短时间时，促进了捕获探针和靶核酸的有效杂交 。

    Methods and compositions to detect nucleic acids in a biological sample
    2.
    发明申请
    Methods and compositions to detect nucleic acids in a biological sample 有权

    公开(公告)号：US20060068417A1

    公开(公告)日：2006-03-30

    申请号：US11173915

    申请日：2005-06-29

    申请人： Michael Becker ,  Mehrdad Majlessi

    发明人： Michael Becker ,  Mehrdad Majlessi

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6837 ,  C12Q1/6834 ,  C12Q2565/525 ,  C12Q2565/501 ,  C12Q2525/301

    摘要： Methods of the invention separate a target nucleic acid from a sample by using at least one capture probe oligonucleotide that contains a target-complementary region and a member of a specific binding pair that attaches the target nucleic acid to an immobilized probe on a capture support, thus forming a capture hybrid that is separated from other sample components before the target nucleic acid is released from the capture support and hybridized to a detection probe to form a detection hybrid that produces a detectable signal that indicates the presence of the target nucleic acid in the sample. Compositions for practicing the methods of the invention include a capture probe oligonucleotide made up a target-complementary region sequence and a covalently linked capture region sequence that includes a member of a specific binding pair.

    Kits containing modified amplification oligonucleotides
    3.
    发明申请
    Kits containing modified amplification oligonucleotides 审中-公开
    试剂盒含有修饰的扩增寡核苷酸

    公开(公告)号：US20050106610A1

    公开(公告)日：2005-05-19

    申请号：US10973162

    申请日：2004-10-26

    申请人： Michael Becker ,  Mehrdad Majlessi ,  Steven Brentano

    发明人： Michael Becker ,  Mehrdad Majlessi ,  Steven Brentano

    IPC分类号： C12N15/09 ,  C07H21/00 ,  C12Q1/68 ,  C12Q1/6832 ,  C12Q1/686 ,  C12Q1/6865 ,  C12P19/34

    CPC分类号： C07H21/00 ,  C12Q1/6832 ,  C12Q1/686 ,  C12Q1/6865 ,  C12Q2527/107 ,  C12Q2525/113 ,  C12Q2523/113 ,  C12Q2537/101 ,  C12Q2565/107

    摘要： The present invention concerns oligonucleotides containing one or more modified nucleotides which increase the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. Such modified oligonucleotides include oligonucleotides containing at least one 2′-O-methylribofuranosyl moiety joined to a nitrogenous base. Oligonucleotides can be modified in accordance with the present invention to preferentially bind RNA targets. The present invention also concerns methods of using these modified oligonucleotides and kits containing the same.

    摘要翻译： 本发明涉及含有一个或多个修饰的核苷酸的寡核苷酸，其增加寡核苷酸与具有互补核苷酸碱基序列的靶核酸的结合亲和力。 这些修饰的寡核苷酸以比具有相同核苷酸碱基序列的未修饰寡核苷酸更快的速率与靶序列杂交。 这种修饰的寡核苷酸包括含有至少一个连接到含氮碱基的2'-O-甲基呋喃核糖基部分的寡核苷酸。 可以根据本发明修饰寡核苷酸以优先结合RNA靶。 本发明还涉及使用这些修饰的寡核苷酸的方法和含有该寡核苷酸的试剂盒。

    Coating based on diamond-like carbon
    5.
    发明授权
    Coating based on diamond-like carbon 有权
    基于类金刚石的涂层

    公开(公告)号：US08911868B2

    公开(公告)日：2014-12-16

    申请号：US13548550

    申请日：2012-07-13

    申请人： Hans-Joachim Scheibe ,  Thomas Schuelke ,  Lars Haubold ,  Michael Becker ,  Yaran Mahmut

    发明人： Hans-Joachim Scheibe ,  Thomas Schuelke ,  Lars Haubold ,  Michael Becker ,  Yaran Mahmut

    IPC分类号： C23C14/00 ,  C23C14/06 ,  C23C14/32

    CPC分类号： C23C14/0021 ,  C23C14/0605 ,  C23C14/325 ,  Y10T428/24529 ,  Y10T428/2495 ,  Y10T428/24975 ,  Y10T428/265 ,  Y10T428/30

    摘要： A coating based on diamond-like is formed from a plurality of films of diamond-like carbon formed alternatingly over one another and in this respect a film in which no portion or only a much lower portion of doped fluorine is contained. A film in which fluorine or at least fluorine with a higher portion than the film arranged thereunder or thereabove are formed alternatingly over one another. The coating could be manufactured by using a target of pure carbon. Films are deposited on a surface of a substrate by means of a PVD process, with the portion of fluorine contained in doped form. Films are formed alternately being varied by varying a supplied volume flow of a fluorine/carbon compound or sulfur/fluorine compound as a precursor.

    摘要翻译： 基于金刚石样的涂层由多个彼此交替形成的类金刚石碳膜形成，并且在这方面形成其中不含有部分或仅含有较低部分掺杂氟的膜。 在其上交替地形成有氟或至少具有比其下面布置的膜更高的氟的膜的膜。 可以通过使用纯碳的目标来制造涂层。 通过PVD工艺将膜沉积在衬底的表面上，其中部分氟以掺杂形式包含。 通过改变作为前体的氟/碳化合物或硫/氟化合物的供给体积流动来交替地形成膜。

    Tube for the enteral feeding of a patient
    8.
    发明申请
    Tube for the enteral feeding of a patient 有权
    用于肠内喂养的管子

    公开(公告)号：US20120302962A1

    公开(公告)日：2012-11-29

    申请号：US13478268

    申请日：2012-05-23

    申请人： Michael Becker ,  Barbara Breuer-Thal ,  Katharina Muennich

    发明人： Michael Becker ,  Barbara Breuer-Thal ,  Katharina Muennich

    IPC分类号： A61M39/00

    CPC分类号： A61J15/0015 ,  A61J15/0026 ,  A61J15/0038 ,  A61J15/0065 ,  A61J15/0069

    摘要： A tube unit for the enteral feeding of a patient, with a tube segment that extends along at least a segment of the axial direction (S) and a retention device on the distal end of the tube segment, which extends in at least one direction radially to axial direction (S) beyond the distal end of the tube segment in an extended position and which has a smaller diameter in an elongated position, in which the retention device is elongated along axial direction (S). The retention device moves from an extended position into the elongated position is supported by a control device.

    摘要翻译： 一种用于对患者进行肠内喂养的管单元，具有沿着轴向方向（S）的至少一段延伸的管段和在管段的远端上延伸的保持装置，该保持装置在至少一个方向上沿径向延伸 （S）超过管段的远端延伸，并且在细长位置具有较小直径，其中保持装置沿轴向方向（S）伸长。 保持装置从延伸位置移动到细长位置由控制装置支撑。

    Apparatus for combining components under sterile conditions
    9.
    发明授权
    Apparatus for combining components under sterile conditions 有权
    用于在无菌条件下组合组分的装置

    公开(公告)号：US08172824B2

    公开(公告)日：2012-05-08

    申请号：US10231151

    申请日：2002-08-30

    申请人： Thomas Pfeifer ,  Dietmar Palm ,  Michael Becker ,  Michael Seipp ,  Rainer Wabnegg

    发明人： Thomas Pfeifer ,  Dietmar Palm ,  Michael Becker ,  Michael Seipp ,  Rainer Wabnegg

    IPC分类号： A61B19/00

    CPC分类号： A61J1/2089 ,  A61J1/201 ,  A61J1/2013 ,  A61J1/2055

    摘要： An apparatus for combining under sterile conditions a liquid component held in a first container and a solid or liquid component held in a second container is provided. In one embodiment, two hollow bodies for receiving the two containers are provided, the two hollow bodies being arranged one within the other and being slidable relative to one another in a guided manner in the longitudinal direction. A cannula holder forms a pot-shaped structural unit with the first hollow body. Alternatively, the cannula holder is provided with at least one cannula. The cannula holder and the first hollow body may be separate components, in which the cannula holder is pot-shaped.

    摘要翻译： 提供了一种用于在无菌条件下组合保持在第一容器中的液体组分和固定在第二容器中的固体或液体组分的装置。 在一个实施例中，提供了两个用于容纳两个容器的中空体，两个中空体一个设置在另一个中，并且可以在纵向方向上以引导方式相对于彼此滑动。 套管支架与第一中空体形成盆形结构单元。 或者，套管保持器设置有至少一个插管。 套管保持器和第一中空体可以是分离的部件，其中套管保持器是壶形的。