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公开(公告)号：US20130260368A1
公开(公告)日：2013-10-03
申请号：US13823551
申请日：2011-09-16
申请人： Reinhold Pollner , Mehrdad Majlessi , Susan Yamagata , Michael M. Becker , Mark Reynolds , Lyle Arnold
发明人： Reinhold Pollner , Mehrdad Majlessi , Susan Yamagata , Michael M. Becker , Mark Reynolds , Lyle Arnold
IPC分类号： C12Q1/70
CPC分类号： C12Q1/707 , C12Q1/6837 , C12Q1/703 , Y02A50/53 , Y02A50/54 , C12Q2525/161
摘要： The invention provides chimeric capture probes immobilizable via an L-nucleic acid tail that can bind to a complementary L-nucleic acid in an immobilized probe. The capture probes are useful for capturing a target nucleic acid from a sample. The L-nucleic acid in the tail of the capture probe bind to the complementary L-nucleic acid in the immobilized probe with similar affinity as would otherwise equivalent D-nucleic acids. However, the L-nucleic acid of the capture probe tail and immobilized probes do not form stable duplexes with D-nucleic acids present in the sample containing the target nucleic acid. Binding of nucleic acids in the sample directly to immobilized probe or to the tail of the capture probe is reduced or eliminated increasing the sensitivity and/or specificity of the assay.
摘要翻译： 本发明提供了通过可以在固定化探针中结合互补L-核酸的L-核酸尾部固定的嵌合捕获探针。 捕获探针可用于从样品中捕获靶核酸。 捕获探针尾部的L-核酸与固定化探针中的互补L-核酸结合，具有与否则相当于D-核酸的亲和力。 然而，捕获探针尾和固定化探针的L-核酸不与存在于含有靶核酸的样品中的D-核酸形成稳定的双链体。 将样品中的核酸直接与捕获探针的固定化探针或尾部的结合降低或消除，增加了测定的灵敏度和/或特异性。
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公开(公告)号：US20080114161A1
公开(公告)日：2008-05-15
申请号：US11930913
申请日：2007-10-31
IPC分类号： C07H21/04
CPC分类号： C12Q1/6832 , C07H21/00 , C12Q1/6816 , C12Q1/6844 , C12Q1/686 , C12Q1/6865 , C12Q2563/131 , C12Q2563/113 , C12Q2525/101 , C12Q2525/117 , C12Q2527/107
摘要： The present invention concerns oligonucleotides containing one or more modified nucleotides which increase the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. Such modified oligonucleotides include oligonucleotides containing at least one 2′-methylribofuranosyl moiety joined to a nitrogenous base. Oligonucleotides can be modified in accordance with the present invention to preferentially bind RNA targets. The present invention also concerns methods of using these modified oligonucleotides and kits containing the same.
摘要翻译： 本发明涉及含有一个或多个修饰的核苷酸的寡核苷酸，其增加寡核苷酸与具有互补核苷酸碱基序列的靶核酸的结合亲和力。 这些修饰的寡核苷酸以比具有相同核苷酸碱基序列的未修饰寡核苷酸更快的速率与靶序列杂交。 这种修饰的寡核苷酸包括含有至少一个连接到含氮碱基的2'-甲基呋喃核糖基部分的寡核苷酸。 可以根据本发明修饰寡核苷酸以优先结合RNA靶。 本发明还涉及使用这些修饰的寡核苷酸的方法和含有该寡核苷酸的试剂盒。
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3.发明授权Method for determining the presence of an RNA analyte in a sample using a modified oligonucleotide probe 有权使用修饰的寡核苷酸探针测定样品中RNA分析物的存在的方法公开(公告)号：US07070925B1
公开(公告)日：2006-07-04
申请号：US09565427
申请日：2000-05-05
CPC分类号： C12Q1/6832 , C07H21/00 , C12Q1/6816 , C12Q1/6844 , C12Q1/686 , C12Q1/6865 , C12Q2563/131 , C12Q2563/113 , C12Q2525/101 , C12Q2525/117 , C12Q2527/107
摘要： The present invention concerns oligonucleotides containing one or more modified nucleotides which increase the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. Such modified oligonucleotides include oligonucleotides containing at least one 2′-O-methylribofuranosyl moiety joined to a nitrogenous base. Oligonucleotides can be modified in accordance with the present invention to preferentially bind RNA targets. The present invention also concerns methods of using these modified oligonucleotides and kits containing the same.
摘要翻译： 本发明涉及含有一个或多个修饰的核苷酸的寡核苷酸，其增加寡核苷酸与具有互补核苷酸碱基序列的靶核酸的结合亲和力。 这些修饰的寡核苷酸以比具有相同核苷酸碱基序列的未修饰寡核苷酸更快的速率与靶序列杂交。 这种修饰的寡核苷酸包括含有至少一个连接到含氮碱基的2'-O-甲基呋喃核糖基部分的寡核苷酸。 可以根据本发明修饰寡核苷酸以优先结合RNA靶。 本发明还涉及使用这些修饰的寡核苷酸的方法和含有该寡核苷酸的试剂盒。
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公开(公告)号：US20080090247A1
公开(公告)日：2008-04-17
申请号：US11925209
申请日：2007-10-26
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6832 , C07H21/00 , C12Q1/6816 , C12Q1/6844 , C12Q1/686 , C12Q1/6865 , C12Q2563/131 , C12Q2563/113 , C12Q2525/101 , C12Q2525/117 , C12Q2527/107
摘要： The present invention concerns oligonucleotides containing one or more modified nucleotides which increase the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. Such modified oligonucleotides include oligonucleotides containing at least one 2′-O-methylribofuranosyl moiety joined to a nitrogenous base. Oligonucleotides can be modified in accordance with the present invention to preferentially bind RNA targets. The present invention also concerns methods of using these modified oligonucleotides and kits containing the same.
摘要翻译： 本发明涉及含有一个或多个修饰的核苷酸的寡核苷酸，其增加寡核苷酸与具有互补核苷酸碱基序列的靶核酸的结合亲和力。 这些修饰的寡核苷酸以比具有相同核苷酸碱基序列的未修饰寡核苷酸更快的速率与靶序列杂交。 这种修饰的寡核苷酸包括含有至少一个连接到含氮碱基的2'-O-甲基呋喃核糖基部分的寡核苷酸。 可以根据本发明修饰寡核苷酸以优先结合RNA靶。 本发明还涉及使用这些修饰的寡核苷酸的方法和含有该寡核苷酸的试剂盒。
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公开(公告)号：US20080090246A1
公开(公告)日：2008-04-17
申请号：US11924910
申请日：2007-10-26
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6832 , C07H21/00 , C12Q1/6816 , C12Q1/6844 , C12Q1/686 , C12Q1/6865 , C12Q2563/131 , C12Q2563/113 , C12Q2525/101 , C12Q2525/117 , C12Q2527/107
摘要： The present invention concerns oligonucleotides containing one or more modified nucleotides which increase the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. Such modified oligonucleotides include oligonucleotides containing at least one 2′-O-methylribofuranosyl moiety joined to a nitrogenous base. Oligonucleotides can be modified in accordance with the present invention to preferentially bind RNA targets. The present invention also concerns methods of using these modified oligonucleotides and kits containing the same.
摘要翻译： 本发明涉及含有一个或多个修饰的核苷酸的寡核苷酸，其增加寡核苷酸与具有互补核苷酸碱基序列的靶核酸的结合亲和力。 这些修饰的寡核苷酸以比具有相同核苷酸碱基序列的未修饰寡核苷酸更快的速率与靶序列杂交。 这种修饰的寡核苷酸包括含有至少一个连接到含氮碱基的2'-O-甲基呋喃核糖基部分的寡核苷酸。 可以根据本发明修饰寡核苷酸以优先结合RNA靶。 本发明还涉及使用这些修饰的寡核苷酸的方法和含有该寡核苷酸的试剂盒。
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公开(公告)号：US20060068417A1
公开(公告)日：2006-03-30
申请号：US11173915
申请日：2005-06-29
申请人： Michael Becker , Mehrdad Majlessi
发明人： Michael Becker , Mehrdad Majlessi
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12Q1/6834 , C12Q2565/525 , C12Q2565/501 , C12Q2525/301
摘要： Methods of the invention separate a target nucleic acid from a sample by using at least one capture probe oligonucleotide that contains a target-complementary region and a member of a specific binding pair that attaches the target nucleic acid to an immobilized probe on a capture support, thus forming a capture hybrid that is separated from other sample components before the target nucleic acid is released from the capture support and hybridized to a detection probe to form a detection hybrid that produces a detectable signal that indicates the presence of the target nucleic acid in the sample. Compositions for practicing the methods of the invention include a capture probe oligonucleotide made up a target-complementary region sequence and a covalently linked capture region sequence that includes a member of a specific binding pair.
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7.发明授权Kits for amplifying target nucleic acid sequences using modified oligonucleotides 有权使用修饰的寡核苷酸扩增靶核酸序列的试剂盒公开(公告)号：US06903206B1
公开(公告)日：2005-06-07
申请号：US09523237
申请日：2000-03-10
CPC分类号： C07H21/00 , C12Q1/6832 , C12Q1/686 , C12Q1/6865 , C12Q2527/107 , C12Q2525/113 , C12Q2523/113 , C12Q2537/101 , C12Q2565/107
摘要： The present invention concerns oligonucleotides containing one or more modified nucleotides which increase the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. Such modified oligonucleotides include oligonucleotides containing at least one 2′-O-methylribofuranosyl moiety joined to a nitrogenous base. Oligonucleotides can be modified in accordance with the present invention to preferentially bind RNA targets. The present invention also concerns methods of using these modified oligonucleotides and kits containing the same.
摘要翻译： 本发明涉及含有一个或多个修饰的核苷酸的寡核苷酸，其增加寡核苷酸与具有互补核苷酸碱基序列的靶核酸的结合亲和力。 这些修饰的寡核苷酸以比具有相同核苷酸碱基序列的未修饰寡核苷酸更快的速率与靶序列杂交。 这种修饰的寡核苷酸包括含有至少一个连接到含氮碱基的2'-O-甲基呋喃核糖基部分的寡核苷酸。 可以根据本发明修饰寡核苷酸以优先结合RNA靶。 本发明还涉及使用这些修饰的寡核苷酸的方法和含有该寡核苷酸的试剂盒。
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公开(公告)号：US20050106610A1
公开(公告)日：2005-05-19
申请号：US10973162
申请日：2004-10-26
IPC分类号： C12N15/09 , C07H21/00 , C12Q1/68 , C12Q1/6832 , C12Q1/686 , C12Q1/6865 , C12P19/34
CPC分类号： C07H21/00 , C12Q1/6832 , C12Q1/686 , C12Q1/6865 , C12Q2527/107 , C12Q2525/113 , C12Q2523/113 , C12Q2537/101 , C12Q2565/107
摘要： The present invention concerns oligonucleotides containing one or more modified nucleotides which increase the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. Such modified oligonucleotides include oligonucleotides containing at least one 2′-O-methylribofuranosyl moiety joined to a nitrogenous base. Oligonucleotides can be modified in accordance with the present invention to preferentially bind RNA targets. The present invention also concerns methods of using these modified oligonucleotides and kits containing the same.
摘要翻译： 本发明涉及含有一个或多个修饰的核苷酸的寡核苷酸，其增加寡核苷酸与具有互补核苷酸碱基序列的靶核酸的结合亲和力。 这些修饰的寡核苷酸以比具有相同核苷酸碱基序列的未修饰寡核苷酸更快的速率与靶序列杂交。 这种修饰的寡核苷酸包括含有至少一个连接到含氮碱基的2'-O-甲基呋喃核糖基部分的寡核苷酸。 可以根据本发明修饰寡核苷酸以优先结合RNA靶。 本发明还涉及使用这些修饰的寡核苷酸的方法和含有该寡核苷酸的试剂盒。
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公开(公告)号：US06280952B1
公开(公告)日：2001-08-28
申请号：US09574561
申请日：2000-05-19
IPC分类号： C12Q168
CPC分类号： C12Q1/6834 , C12Q1/6813
摘要： A method for capturing a target polynucleotide in a sample onto a solid support with an attached immobilized probe by using a capture probe and two different hybridization conditions, which preferably differ in temperature only, is disclosed. The two hybridization conditions control the order of hybridization, where the first hybridization conditions allow hybridization of the capture probe to the target polynucleotide, and the second hybridization conditions allow hybridization of the capture probe to the immobilized probe. The method may be used to detect the presence of a target polynucleotide in a sample by detecting the captured target polynucleotide or amplified target polynucleotide.
摘要翻译： 公开了一种通过使用捕获探针和两种不同的杂交条件（其优选温度不同）将样品中的靶多核苷酸捕获到具有附着的固定化探针的固体支持物上的方法。 两个杂交条件控制杂交的顺序，其中第一杂交条件允许捕获探针与靶多核苷酸杂交，第二杂交条件允许捕获探针与固定的探针杂交。 该方法可以通过检测捕获的靶多核苷酸或扩增的靶多核苷酸来检测样品中靶多核苷酸的存在。
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公开(公告)号：US09938590B2
公开(公告)日：2018-04-10
申请号：US13823551
申请日：2011-09-16
申请人： Reinhold Pollner , Mehrdad Majlessi , Susan Yamagata , Michael M. Becker , Mark Reynolds , Lyle Arnold
发明人： Reinhold Pollner , Mehrdad Majlessi , Susan Yamagata , Michael M. Becker , Mark Reynolds , Lyle Arnold
CPC分类号： C12Q1/707 , C12Q1/6837 , C12Q1/703 , Y02A50/53 , Y02A50/54 , C12Q2525/161
摘要： The invention provides chimeric capture probes immobilizable via an L-nucleic acid tail that can bind to a complementary L-nucleic acid in an immobilized probe. The capture probes are useful for capturing a target nucleic acid from a sample. The L-nucleic acid in the tail of the capture probe bind to the complementary L-nucleic acid in the immobilized probe with similar affinity as would otherwise equivalent D-nucleic acids. However, the L-nucleic acid of the capture probe tail and immobilized probes do not form stable duplexes with D-nucleic acids present in the sample containing the target nucleic acid. Binding of nucleic acids in the sample directly to immobilized probe or to the tail of the capture probe is reduced or eliminated increasing the sensitivity and/or specificity of the assay.
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