公开(公告)号：US20160266020A1

公开(公告)日：2016-09-15

申请号：US15036811

申请日：2014-11-13

Applicant: RANDOX LABORATORIES LTD

Inventor： Michael Veitinger ,  Maria Zellner

IPC: G01N1/40 ,  C12Q1/48

CPC classification number: G01N1/4077 ,  C12Q1/485 ,  G01N33/6842 ,  G01N33/86

Abstract: The present invention provides a novel method of in vitro sample preparation for the analysis of proteins. In a first aspect, the present invention is a method comprising the separation and individual treatment of blood platelets and platelet-poor plasma obtained from a platelet-rich plasma sample. This allows for optimal treatment of both fractions and overcomes many of the problems associated with current methods. In a second aspect of the present invention the platelet sample preparation method can be implemented before a biochip based immunoassay for the detection of platelet proteins in combination with plasma proteins.

Abstract translation: 本发明提供了用于分析蛋白质的体外样品制备的新方法。 在第一方面，本发明是一种包括从富含血小板的血浆样品中分离和单独处理血小板和血小板不足血浆的方法。 这允许对两个级分进行最佳处理并克服与当前方法相关的许多问题。 在本发明的第二方面，血小板样品制备方法可以在用于检测与血浆蛋白质组合的血小板蛋白质的基于生物芯片的免疫测定之前实施。

公开(公告)号：US09927429B2

公开(公告)日：2018-03-27

申请号：US14411496

申请日：2013-07-08

Applicant: Randox Laboratories Ltd.

Inventor： Maria Zellner ,  Ellen Umlauf

IPC: G01N33/53 ,  G01N33/566 ,  C07K16/00 ,  G01N33/68

CPC classification number: G01N33/5308 ,  G01N33/6896 ,  G01N2333/4712 ,  G01N2800/2814 ,  G01N2800/2821

Abstract: The present invention provides an in vitro method to aid the diagnosis of Alzheimer's disease (AD) or Mild Cognitive Impairment (MCI), comprising determining the level of expression of one or more tropomyosin isoform(s) corresponding to P09493-3 and/or P09493-1 in a patient's sample. A kit comprising probes that bind to tropomyosin isoforms P09493-3 and P09493-1 is also provided.

公开(公告)号：US09880178B2

公开(公告)日：2018-01-30

申请号：US14431557

申请日：2013-10-03

Applicant: Randox Laboratories Ltd.

Inventor： Ellen Umlauf ,  Maria Zellner

IPC: G01N33/53 ,  G01N33/567 ,  G01N33/68 ,  G06F19/18 ,  G06F19/00

CPC classification number: G01N33/6896 ,  G01N2333/4712 ,  G01N2333/775 ,  G01N2333/90 ,  G01N2333/90638 ,  G01N2333/91085 ,  G01N2800/2821 ,  G06F19/18 ,  G16H50/20

Abstract: The present invention provides an ex vivo method for aiding the diagnosis of Alzheimer's disease in a patient comprising: (i) determining the number of alleles of ApoE4 in the patient's genome; (ii) determining the combined expression level of at least three platelet proteins in a platelet sample from the patient; and (iii) comparing the resulting value of step (ii) to a control value, wherein the at least three platelet proteins include at least one isoform of alpha-tropomyosin containing exon 1a and at least two platelet proteins selected from monoamine oxidase-B, coagulation factor XIIIa, wild-type GSTO-1 or mutant GSTO-1, wherein a result higher than the control value is indicative of Alzheimer's disease.The invention also provides a solid support comprising one or more ligands of at least one isoform of alpha-tropomyosin containing exon 1a, and one or more ligands of at least two platelet proteins selected from monoamine oxidase-B, coagulation factor XIIIa, wild-type GSTO-1 protein and/or mutant GSTO-1 protein immobilized thereon.

公开(公告)号：US20150198590A1

公开(公告)日：2015-07-16

申请号：US14411496

申请日：2013-07-08

Applicant: Randox Laboratories Ltd.

Inventor： Maria Zellner ,  Ellen Umlauf

IPC: G01N33/53

CPC classification number: G01N33/5308 ,  G01N33/6896 ,  G01N2333/4712 ,  G01N2800/2814 ,  G01N2800/2821

Abstract: The present invention provides an in vitro method to aid the diagnosis of Alzheimer's disease (AD) or Mild Cognitive Impairment (MCI), comprising determining the level of expression of one or more tropomyosin isoform(s) corresponding to P09493-3 and/or P09493-1 in a patient's sample. A kit comprising probes that bind to tropomyosin isoforms P09493-3 and P09493-1 is also provided

Abstract translation: 本发明提供了一种有助于诊断阿尔茨海默病（AD）或轻度认知障碍（MCI）的体外方法，其包括确定对应于P09493-3和/或P09493的一种或多种原肌球蛋白同种型的表达水平 -1在患者的样品中。 还提供了包含结合原肌球蛋白异构体P09493-3和P09493-1的探针的试剂盒

公开(公告)号：US10209167B2

公开(公告)日：2019-02-19

申请号：US15036811

申请日：2014-11-13

Applicant: Randox Laboratories Ltd.

Inventor： Michael Veitinger ,  Maria Zellner

IPC: G01N1/40 ,  C12Q1/48 ,  G01N33/68 ,  G01N33/86

Abstract: The present invention provides a novel method of in vitro sample preparation for the analysis of proteins. In a first aspect, the present invention is a method comprising the separation and individual treatment of blood platelets and platelet-poor plasma obtained from a platelet-rich plasma sample. This allows for optimal treatment of both fractions and overcomes many of the problems associated with current methods. In a second aspect of the present invention the platelet sample preparation method can be implemented before a biochip based immunoassay for the detection of platelet proteins in combination with plasma proteins.

公开(公告)号：US20150247870A1

公开(公告)日：2015-09-03

申请号：US14431557

申请日：2013-10-03

Applicant: RANDOX LABORATORIES LTD

Inventor： Ellen Umlauf ,  Maria Zellner

IPC: G01N33/68 ,  G06F19/00 ,  G06F19/18

CPC classification number: G01N33/6896 ,  G01N2333/4712 ,  G01N2333/775 ,  G01N2333/90 ,  G01N2333/90638 ,  G01N2333/91085 ,  G01N2800/2821 ,  G06F19/18 ,  G16H50/20

Abstract: The present invention provides an ex vivo method for aiding the diagnosis of Alzheimer's disease in a patient comprising: (i) determining the number of alleles of ApoE4 in the patient's genome; (ii) determining the combined expression level of at least three platelet proteins in a platelet sample from the patient; and (iii) comparing the resulting value of step (ii) to a control value, wherein the at least three platelet proteins include at least one isoform of alpha-tropomyosin containing exon 1a and at least two platelet proteins selected from monoamine oxidase-B, coagulation factor XIIIa, wild-type GSTO-1 or mutant GSTO-1, wherein a result higher than the control value is indicative of Alzheimer's disease.The invention also provides a solid support comprising one or more ligands of at least one isoform of alpha-tropomyosin containing exon 1a, and one or more ligands of at least two platelet proteins selected from monoamine oxidase-B, coagulation factor XIIIa, wild-type GSTO-1 protein and/or mutant GSTO-1 protein immobilised thereon.

Abstract translation: 本发明提供一种用于辅助患者中阿尔茨海默病诊断的离体方法，包括：（i）确定患者基因组中ApoE4的等位基因数; （ii）确定来自患者的血小板样品中至少三种血小板蛋白的组合表达水平; 和（iii）将步骤（ii）的所得值与对照值进行比较，其中所述至少三种血小板蛋白包括至少一种含有外显子1a的α-原肌球蛋白的同种型和至少两种选自单胺氧化酶-B的血小板蛋白， 凝血因子XIIIa，野生型GSTO-1或突变型GSTO-1，其中高于对照值的结果指示阿尔茨海默病。 本发明还提供了一种固体支持物，其包含一种或多种至少一种含有α-原肌球蛋白的外显子1a同种型的配体，以及一种或多种选自单胺氧化酶-B，凝血因子XIIIa，野生型的至少两种血小板蛋白的配体 固定在其上的GSTO-1蛋白和/或突变型GSTO-1蛋白。

公开(公告)号：US20140336141A1

公开(公告)日：2014-11-13

申请号：US14367498

申请日：2012-12-21

Applicant: RANDOX LABORATORIES LTD.

Inventor： Maria Zellner ,  Ellen Umlauf

IPC: A61K31/7076 ,  C12Q1/68 ,  A61K31/519 ,  A61K31/4415 ,  A61K31/714

CPC classification number: A61K31/7076 ,  A61K31/4415 ,  A61K31/519 ,  A61K31/714 ,  C12Q1/6881 ,  C12Q2600/136 ,  G01N33/5023 ,  G01N2333/90638 ,  G01N2800/2821

Abstract: The invention exploits the elucidation of a connection between the S-adenosylmethionine one-carbon cycle and MAO-B levels and describes drug efficacy testing methods and the use of compounds of the S-adenosylmethionine one-carbon cycle as MAO-B inhibitors in disease prevention and treatment.

Abstract translation: 本发明利用了S-腺苷甲硫氨酸一碳循环与MAO-B水平的联系，阐述了药物疗效检测方法和S-腺苷甲硫氨酸一碳循环的化合物作为MAO-B抑制剂在疾病预防中的应用 和治疗。