公开(公告)号：US20180039059A1

公开(公告)日：2018-02-08

申请号：US15645175

申请日：2017-07-10

Applicant: OLYMPUS CORPORATION

Inventor： Kanako KONDO ,  Nobuhiro TAKAMIZAWA

IPC: G02B21/36 ,  G02B21/00 ,  G02B21/16 ,  G02B21/26 ,  G02B21/24 ,  G02B21/02 ,  G02B21/06

Abstract: Provided is a microscope system including: a plurality of objective lenses that are selectively used to collect fluorescence from a specimen; a detecting unit that obtains image information of the specimen on the basis of the fluorescence collected by the objective lens; and an adjustment unit that adjusts the observation range of the specimen S and that divides the observation range into one or more observation regions corresponding to a capture range captured by the detecting unit, wherein the adjustment unit calculates an observation magnification at which the entire well is included in the observation range, the number of observation regions is minimized, and the resolution becomes the highest, on the basis of information about the size of the well in a multi-plate for accommodating the specimen and information about an objective magnification of the objective lens, and adjusts the observation range on the basis of the calculated observation magnification.

公开(公告)号：US20160004056A1

公开(公告)日：2016-01-07

申请号：US14789167

申请日：2015-07-01

Applicant: OLYMPUS CORPORATION

Inventor： Nobuhiro TAKAMIZAWA

IPC: G02B21/00 ,  G02B21/02 ,  G06T3/40 ,  G02B27/58

CPC classification number: G02B21/008 ,  G02B21/0048 ,  G02B21/025 ,  G02B27/58 ,  G06T3/4053

Abstract: It is possible to easily acquire an original image of a specimen that satisfies necessary conditions for generating a super-resolution image. The present invention provides a scanning microscope apparatus that includes an objective lens that irradiates a specimen with laser light emitted from a laser light source; a scanning portion that scans the laser light irradiated onto the specimen via the objective lens; and a PC that acquires an original image of the specimen, calculates, from the magnification of the objective lens, information about the number of pixels of the original image or information about the zoom magnification of the original image that achieves the per-pixel pixel resolution needed to generate a super-resolution image, and controls the scanning portion or image acquisition according to the calculated information about the number of pixels or information about the zoom magnification.

Abstract translation: 可以容易地获取满足用于生成超分辨率图像的必要条件的样本的原始图像。 本发明提供了一种扫描显微镜装置，其包括：用从激光光源发射的激光照射样本的物镜; 扫描部，其经由所述物镜扫描照射到所述标本上的激光; 以及获取样本的原始图像的PC，从物镜的放大倍率计算关于原始图像的像素数量的信息或关于实现每像素像素分辨率的原始图像的变焦倍率的信息 需要产生超分辨率图像，并且根据关于像素数量的计算信息或关于变焦倍率的信息来控制扫描部分或图像采集。

公开(公告)号：US20140340504A1

公开(公告)日：2014-11-20

申请号：US14273235

申请日：2014-05-08

Applicant: OLYMPUS CORPORATION

Inventor： Nobuhiro TAKAMIZAWA

IPC: G02B21/36 ,  H04N5/232 ,  H04N5/225 ,  G02B21/06 ,  G02B21/26

CPC classification number: G02B21/365 ,  G02B21/0076 ,  G02B21/06 ,  G02B21/24 ,  G02B21/26 ,  G02B21/32 ,  H01J37/28 ,  H04N5/2256 ,  H04N5/23229

Abstract: A microscope system including: an observation optical system acquiring an image of an observation position of a sample; a stimulation optical system irradiating the sample with stimulation light; an observation position setting section setting the observation positions; a stimulation position setting section setting a common stimulation position; a stage switching the observation positions; a synchronization condition setting section setting a common synchronization condition in which the timing of the image acquisition is associated with the timing of the irradiation with stimulation light; an observation condition registration section registering the common stimulation position and the common synchronization condition as observation conditions associated with each of the observation positions; and a control section, according to the registered conditions, switching the observation positions, making the observation optical system acquire an image of each of the observation positions, and making the stimulation optical system irradiate each of the stimulation positions with stimulation light.

Abstract translation: 一种显微镜系统，包括：观察光学系统，获取样本的观察位置的图像; 刺激光学系统用刺激光照射样品; 观察位置设定部，设定观察位置; 刺激位置设定部，设定共同的刺激位置; 切换观察位置的阶段; 同步条件设定部，其将图像获取的定时与激励光的照射的定时相关联的公共同步状态; 将共同刺激位置和公共同步条件作为与每个观察位置相关联的观察条件登记的观察条件登记部; 以及根据注册条件的控制部分切换观察位置，使得观察光学系统获取每个观察位置的图像，并且使刺激光学系统用刺激光照射每个刺激位置。

公开(公告)号：US20140247337A1

公开(公告)日：2014-09-04

申请号：US14191161

申请日：2014-02-26

Applicant: OLYMPUS CORPORATION

Inventor： Nobuhiro TAKAMIZAWA

IPC: G02B21/36

CPC classification number: G02B21/008 ,  G02B21/0076

Abstract: Unnecessary degradation of a light detector is prevented. Provided is a microscope apparatus (100) including: a scanner (5) that that performs scanning of illumination light emitted from a light source (3) on a specimen in two directions intersecting each other; an objective lens (7) that collects fluorescence produced in the specimen; a dispersive element (15) that disperses the fluorescence collected by the objective lens (7) into spectral components; a multichannel detector (20) that has a plurality of cells (21) for detecting the spectral components obtained through the dispersion performed by the dispersive element (15); a grouping control section (31) that groups the plurality of cells (21) of the multichannel detector (20) into a used group and an unused group; and a sensitivity control section (33) that turns off the sensitivities of the cells that are grouped into the unused group by the grouping control section (31) or reduces the sensitivities thereof with respect to the sensitivities of the cells that are grouped into the used group.

Abstract translation: 防止了光检测器的不必要的退化。 本发明提供一种显微镜装置（100），包括：扫描仪（5），其对彼此相交的两个方向的从所述样本的光源（3）发出的照明光进行扫描; 收集样品中产生的荧光的物镜（7） 将由物镜（7）收集的荧光分散成光谱分量的色散元件（15） 具有多个单元（21）的多通道检测器（20），用于检测通过色散元件（15）执行的色散获得的光谱分量; 分组控制部分，将多通道检测器（20）的多个单元（21）分组成使用组和未使用组; 以及灵敏度控制部（33），其通过分组控制部（31）关闭被分组为未使用组的单元的灵敏度，或者降低其对分组到所使用的单元中的单元的灵敏度的敏感度 组。

公开(公告)号：US20130314775A1

公开(公告)日：2013-11-28

申请号：US13901411

申请日：2013-05-23

Applicant: OLYMPUS CORPORATION

Inventor： Nobuhiro TAKAMIZAWA

IPC: G02B21/06 ,  G02B21/36

CPC classification number: G02B21/06 ,  G01N21/6458 ,  G01N21/648 ,  G02B21/16 ,  G02B21/18 ,  G02B21/36 ,  G02B21/365

Abstract: This microscope apparatus is provided with the following elements: an objective lens; a CCD that constructs an image of a sample S; an illumination intensity change unit for adjusting an intensity of excitation light; an sensitivity adjustment unit for adjusting a detection sensitivity by the CCD; a galvanometer mirror for changing a light focusing position of excitation light at a pupil position of the objective lens; a storage unit that stores, for each observation method, a predetermined intensity, a predetermined detection sensitivity, and the light focusing position; and a control unit that switches the observation method, reads out the intensity, the detection sensitivity, and the light focusing position according to the observation method based on a synchronization signal synchronized with a frame signal for constructing an image, and controls the illumination intensity change unit, the sensitivity adjustment unit, and the galvanometer mirror.

Abstract translation: 该显微镜装置具有以下元件：物镜; 构建样品S的图像的CCD; 用于调节激发光强度的照明强度变化单元; 用于通过CCD调整检测灵敏度的灵敏度调节单元; 用于改变物镜的光瞳位置处的激发光的聚焦位置的电流计镜; 存储单元，其针对每个观察方法存储预定强度，预定检测灵敏度和所述聚光位置; 以及控制单元，其切换观察方法，根据与用于构建图像的帧信号同步的同步信号的观察方法读出强度，检测灵敏度和聚焦位置，并且控制照明强度变化 单元，灵敏度调节单元和电流计镜。