公开(公告)号：US11279920B2

公开(公告)日：2022-03-22

申请号：US16135370

申请日：2018-09-19

Applicant: NOVOZYMES A/S

Inventor： Ming Li ,  Junxin Duan ,  Zheng Liu ,  Shiro Fukuyama ,  Keiichi Ayabe ,  Guillermo Coward-Kelly ,  Randall Deinhammer

IPC: C12N9/34 ,  C12P7/08 ,  C12P19/02

Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

公开(公告)号：US20200165591A1

公开(公告)日：2020-05-28

申请号：US16080609

申请日：2017-03-01

Applicant: NOVOZYMES A/S

Inventor： Chee-Leong Soong ,  Bernardo Vidal Jr. ,  Shiro Fukuyama ,  Tomoko Matsui ,  Keiichi Ayabe ,  Louis Patrick Lessard ,  Nathaniel E. Kreel ,  Kenneth Jensen ,  Ming Li ,  Wei Peng ,  Lan Tang ,  Xinyan Guo

IPC: C12N9/58 ,  C12N15/80 ,  C12N9/48 ,  C12P7/06 ,  C12P19/04

Abstract: Improved processes for producing ethanol from starch-containing materials by the combined use of at least one endoprotease and at least one exo-protease in an SSF process are disclosed. More particularly the exo-protease should make up at least 5% (w/w) of the protease mixture.

公开(公告)号：US10472617B2

公开(公告)日：2019-11-12

申请号：US15714292

申请日：2017-09-25

Applicant: Novozymes A/S

Inventor： Tomoko Matsui ,  Aki Tomiki ,  Allan Svendsen ,  Hanne Vang Hendriksen ,  Mary Ann Stringer ,  Keiichi Ayabe

IPC: C12N9/82 ,  A23L5/20

Abstract: The present invention relates to asparaginase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. The invention further relates to a process of producing a fermentation product, comprising: liquefying a starch-containing material to dextrins with an alpha-amylase in the presence of an asparaginase of the invention; saccharifying the dextrins to a sugar with a glucoamylase; and fermenting the sugar using a fermenting organism.

公开(公告)号：US09879241B2

公开(公告)日：2018-01-30

申请号：US14426051

申请日：2013-09-06

Applicant: NOVOZYMES A/S

Inventor： Jesper Vind ,  Esben Peter Friis ,  Thomas Agsersten Poulsen ,  Michael Skjøt ,  Peter Kamp Hansen ,  Frank Winther Rasmussen ,  Steen Krogsgaard ,  Joyce Craig ,  Guillermo Coward-Kelly ,  Keiichi Ayabe

IPC: C12N9/24 ,  C12P7/04 ,  C12N9/34 ,  C12N9/30 ,  C12P7/10 ,  C12P19/02 ,  C12P19/24 ,  C12N15/52

CPC classification number: C12N9/2428 ,  C12N9/242 ,  C12N15/52 ,  C12P7/10 ,  C12P19/02 ,  C12P19/24 ,  Y02E50/16

Abstract: The present invention relates to variants of a parent glucoamylase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the glucoamylase variants.

公开(公告)号：US09873869B2

公开(公告)日：2018-01-23

申请号：US14790411

申请日：2015-07-02

Applicant: NOVOZYMES A/S ,  NOVOZYMES NORTH AMERICA, INC.

Inventor： Sara Landvik ,  Marc Dominique Morant ,  Keiichi Ayabe ,  Guillermo Coward-Kelly

IPC: C12N9/34 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12N15/81

CPC classification number: C12N9/2428 ,  C12N15/81 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01003 ,  Y02E50/16 ,  Y02E50/17

Abstract: The present invention relates to polypeptides having glucoamylase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

公开(公告)号：US20150093792A1

公开(公告)日：2015-04-02

申请号：US14552021

申请日：2014-11-24

Applicant: Novozymes A/S ,  Novozymes North America, Inc.

Inventor： Sara Landvik ,  Marc Dominique Morant ,  Keiichi Ayabe ,  Guillermo Coward-Kelly

IPC: C12N9/34 ,  C12P7/10 ,  C12P19/14 ,  C12N9/30

CPC classification number: C12P7/14 ,  C12N9/242 ,  C12N9/2428 ,  C12P7/06 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01001 ,  C12Y302/01003 ,  Y02E50/16 ,  Y02E50/17

Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

公开(公告)号：US20220090041A1

公开(公告)日：2022-03-24

申请号：US17547098

申请日：2021-12-09

Applicant: Novozymes A/S

Inventor： Keiichi Ayabe ,  Tomoko Matsui ,  Aki Tomiki-Hashizume ,  Yuma Kurakata ,  Esben Peter Friis ,  Jens Erik Nielsen ,  Roland Alexander Pache

IPC: C12N9/58 ,  C12P7/06

Abstract: The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of Meripilus giganteus. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and/or increased thermal denaturation temperature, compared to the parent Meripilus giganteus protease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US10927361B2

公开(公告)日：2021-02-23

申请号：US16318496

申请日：2017-07-14

Applicant: NOVOZYMES A/S

Inventor： Keiichi Ayabe ,  Tomoko Matsui ,  Aki Tomiki ,  Yuma Kurakata ,  Esben P. Friis ,  Jens E. Nielsen ,  Roland Alexander Pache

IPC: C12N9/58 ,  C12P7/06

Abstract: The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of Meripilus giganteus. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and/or increased thermal denaturation temperature, compared to the parent Meripilus giganteus protease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US20200172946A1

公开(公告)日：2020-06-04

申请号：US16788950

申请日：2020-02-12

Applicant: Novozymes A/S

Inventor： Shiro Fukuyama ,  Keiichi Ayabe

IPC: C12P19/16 ,  C12P19/02 ,  C12N9/44 ,  C13K1/06 ,  C12N9/34

Abstract: The present invention relates to a method of making glucose syrup from liquefied starch, and to compositions useful therein.

公开(公告)号：US20170175095A1

公开(公告)日：2017-06-22

申请号：US15443931

申请日：2017-02-27

Applicant: Novozymes A/S

Inventor： Ming Li ,  Junxin Duan ,  Zheng Liu ,  Shiro Fukuyama ,  Keiichi Ayabe ,  Guillermo Coward-Kelly ,  Randall Deinhammer

IPC: C12N9/34 ,  C12N1/16

CPC classification number: C12N9/2428 ,  C12P7/06 ,  C12P7/08 ,  C12P19/02 ,  C12P19/14 ,  C12P2203/00 ,  C12Y302/01003 ,  C13K1/06 ,  Y02E50/16 ,  Y02E50/17

Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.