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公开(公告)号：US20230023446A1
公开(公告)日：2023-01-26
申请号：US17781973
申请日：2020-12-16
Applicant: Novozymes A/S
Inventor： Chee-Leong Soong , Yuma Kurakata , Brian Frederick Ohman
Abstract: The present invention relates to processes for producing fermentation products from starch-containing material, wherein a thermostable xylanase that is resistance to inhibition by metal ions in the liquefying starch-containing material is present and/or added during liquefaction.
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公开(公告)号：US11220679B2
公开(公告)日：2022-01-11
申请号：US16635195
申请日：2018-08-06
Applicant: NOVOZYMES A/S
Inventor： Noriko Tsutsumi , Tomoko Matsui , Yuma Kurakata
Abstract: The present invention relates to trehalase variants of the Myceliophthora sepedonium GH37 trehalase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
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公开(公告)号：US20230399632A1
公开(公告)日：2023-12-14
申请号：US18250372
申请日：2021-11-02
Applicant: Novozymes A/S
Inventor： Yuma Kurakata , Aki Tomiki-Hashizume
CPC classification number: C12N9/2428 , C12P19/14
Abstract: The present invention relates to glucoamylase variants having improved thermostability and compositions comprising such variants. The present invention further relates to polynucleotides encoding such variants, vectors and host cells comprising genes encoding such variants, which may also enable the production of such variants. The present invention also relates to methods of liquefying starch-containing materials using or applying the variants or compositions, as well as the saccharification thus produced by the method. The present invention also relates to methods of saccharifying starch-containing materials using or applying the variants or compositions, as well as the saccharides thus produced by the method. The present invention further relates to processes for producing fermentation products from starch-containing or cellulosic-containing material, as well as an enzyme blend or composition, or a recombinant host cell or fermenting organism suitable for use in processes of the invention.
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公开(公告)号：US11447763B2
公开(公告)日：2022-09-20
申请号：US17148790
申请日：2021-01-14
Applicant: NOVOZYMES A/S
Inventor： Keiichi Ayabe , Tomoko Matsui , Aki Tomiki , Yuma Kurakata , Esben P. Friis , Jens E. Nielsen , Roland Alexander Pache
Abstract: The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of Meripilus giganteus. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and/or increased thermal denaturation temperature, compared to the parent Meripilus giganteus protease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
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公开(公告)号：US20210139876A1
公开(公告)日：2021-05-13
申请号：US17148790
申请日：2021-01-14
Applicant: NOVOZYMES A/S
Inventor： Keiichi Ayabe , Tomoko Matsui , Aki Tomiki , Yuma Kurakata , Esben P. Friis , Jens E. Nielsen , Roland Alexander Pache
Abstract: The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of Meripilus giganteus. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and/or increased thermal denaturation temperature, compared to the parent Meripilus giganteus protease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
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Patent Agency Ranking
公开(公告)号：US20190352629A1
公开(公告)日：2019-11-21
申请号：US16318496
申请日：2017-07-14
Applicant: NOVOZYMES A/S
Inventor： Keiichi Ayabe , Tomoko Matsui , Aki Tomiki , Yuma Kurakata , Esben Peter Friis , Jens Erik Nielsen , Roland Alexander Pache
Abstract: The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of Meripilus giganteus. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and/or increased thermal denaturation temperature, compared to the parent Meripilus giganteus protease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
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公开(公告)号：US20220154226A1
公开(公告)日：2022-05-19
申请号：US17440477
申请日：2020-03-17
Applicant: Novozymes A/S
Inventor： Takashi Nakanishi , Aki Tomiki , Yuma Kurakata
IPC: C12P7/06
Abstract: The present invention relates to a variant pullulanase, having increased thermo-stability and/or increased thermo-activity compared to a parent pullulanase, comprising a substitution at least a one position selected from a position corresponding to positions 432, 486, 370, 17, 77, 103, 106, 107, 190, 196, 197, 262, 279, 283, 321, 367, 375, 382, 399, 401, 402, 411, 412, 434, 435, 443, 446, 459, 460, 479, 490, 498, 514, 529, 531, 533, 541, 545, 581, 583, 595, 649, 665, 688, 700, 709, 804, 811 of SEQ ID NO: 1, and optionally a deletion of one or more, e.g., all amino acids at positions 821, 822, 823, 824, 825, 826, 827, and 828, wherein the variant has pullulanase activity, and wherein the variant has at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to a parent alpha amylase selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5. Further aspect the present invention relates to a process for liquefying starch-containing material at a temperature above the initial gelatinization temperature using an alpha-amylase and a thermo-stable pullulanase of the invention.
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公开(公告)号：US20220090041A1
公开(公告)日：2022-03-24
申请号：US17547098
申请日：2021-12-09
Applicant: Novozymes A/S
Inventor： Keiichi Ayabe , Tomoko Matsui , Aki Tomiki-Hashizume , Yuma Kurakata , Esben Peter Friis , Jens Erik Nielsen , Roland Alexander Pache
Abstract: The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of Meripilus giganteus. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and/or increased thermal denaturation temperature, compared to the parent Meripilus giganteus protease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
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公开(公告)号：US20210087543A1
公开(公告)日：2021-03-25
申请号：US16635195
申请日：2018-08-06
Applicant: NOVOZYMES A/S
Inventor： Noriko Tsutsumi , Tomoko Matsui , Yuma Kurakata
Abstract: The present invention relates to trehalase variants of the Myceliophthora sepedonium GH37 trehalase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
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公开(公告)号：US10927361B2
公开(公告)日：2021-02-23
申请号：US16318496
申请日：2017-07-14
Applicant: NOVOZYMES A/S
Inventor： Keiichi Ayabe , Tomoko Matsui , Aki Tomiki , Yuma Kurakata , Esben P. Friis , Jens E. Nielsen , Roland Alexander Pache
Abstract: The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of Meripilus giganteus. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and/or increased thermal denaturation temperature, compared to the parent Meripilus giganteus protease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
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