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1.发明申请公开(公告)号：US20130095511A1
公开(公告)日：2013-04-18
申请号：US13582372
申请日：2011-06-10
IPC分类号： C12Q1/18
CPC分类号： C12Q1/18 , C12Q1/34 , G01N33/6851 , G01N2333/986 , G01N2800/26 , G01N2800/44
摘要： The invention relates to the determination of resistances of microorganisms which produce β-lactamases, in particular “extended spectrum β-lactamases” (ESBL). The invention provides a method whereby the microbial resistance can be measured very simply and quickly by means of the catalytic effect of the microbially produced β-lactamases on β-lactam antibiotics, which consists in a hydrolytic cleavage of the β-lactam ring. The method determines the resistance of the bacteria a few hours after a suitable substrate, either a β-lactam antibiotic or a customized β-lactam derivative, has been added to a suspension of the microbes, by direct mass spectrometric measurement of the substrate breakdown caused by the β-lactamases.
摘要翻译： 本发明涉及测定产生β-内酰胺酶的微生物的抗性，特别是“延长光谱β-内酰胺酶”（ESBL）。 本发明提供了一种通过微生物产生的β-内酰胺酶对β-内酰胺抗生素的催化作用非常简单和快速地测量微生物抗性的方法，其中β-内酰胺抗生素包括β-内酰胺环的水解裂解。 该方法通过直接质谱测量引起的底物破坏，在合适的底物（β-内酰胺抗生素或定制的β-内酰胺衍生物）已添加到微生物的悬浮液中后几小时确定细菌的抗性 由β-内酰胺酶。
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公开(公告)号：US08142996B2
公开(公告)日：2012-03-27
申请号：US12224388
申请日：2007-02-15
CPC分类号： G01N33/6848 , C12Q1/37 , C12Q1/56 , G01N2333/75 , G01N2333/976
摘要： The invention relates to the determination of the nature and strength of enzymatic activity in blood using mass spectrometric measurement of a profile of the reaction products. The determination of the enzymatic activity can be used for medical diagnostics, for example, and also to check the effectiveness of medication. The invention provides a method whereby adding probe substances usually not present in blood offers standardized substrates for measuring the enzymatic activity. The probe substances may be added to whole blood, plasma, or serum. The mass spectrometric measurement of the reaction products, after their reversible immobilization on actively binding surfaces of solids, for example, can deliver biomarker patterns of the reaction products which may be indicators for metabolic anomalies or diseases, since these are often accompanied by the formation or activation of characteristic enzymes.
摘要翻译： 本发明涉及使用反应产物分布的质谱测量来测定血液中酶活性的性质和强度。 酶活性的测定可以用于医学诊断，例如，以及检查药物的有效性。 本发明提供了通过添加通常不存在于血液中的探针物质来提供用于测量酶活性的标准化底物的方法。 探针物质可以添加到全血，血浆或血清中。 反应产物在其可固定地固定在固体的活性结合表面上之后的质谱测量可以提供反应产物的生物标志物模式，其可以是代谢异常或疾病的指标，因为它们通常伴随着形成或 特征酶的活化。
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公开(公告)号：US10006076B2
公开(公告)日：2018-06-26
申请号：US14113354
申请日：2011-07-29
CPC分类号： C12Q1/04 , G01N21/77 , G01N33/6851 , G01N2021/7786 , G01N2800/26
摘要： The invention relates to methods and instruments for the rapid detection and rapid mass spectrometric identification of microbial infective agents in blood or other body fluids. The invention recognizes that blood is not a good environment for the cultivation of microbes and provides a method which (a) largely destroys or dissolves the human particles in body fluids, such as erythrocytes and leukocytes in blood, without impairing the ability of the microbes to reproduce, (b) separates the microbial pathogens from the fluid, (c) cultivates them in a nutrient broth which contains none of the antimicrobial components of the body fluids, (d) separates them from the nutrient broth, and (e) identifies the microbes by a mass spectrum of the microbial proteins. The dissolution of the human particles also releases the microbes nesting in macrophages. The cultivation in an optically clear nutrient broth with optimum composition not only accelerates the propagation of the microbes compared to all other cultivation methods, but also makes it possible to continuously measure their quantitative growth starting from a low microbe density. This firstly allows the mass spectrometric identification to be carried out at the earliest possible time, secondly provides a positive detection of microbes far ahead of their identification, which can be lifesaving for the patient; and thirdly makes it possible to start the determination of resistances early.
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公开(公告)号：US20090305327A1
公开(公告)日：2009-12-10
申请号：US12224388
申请日：2007-02-15
CPC分类号： G01N33/6848 , C12Q1/37 , C12Q1/56 , G01N2333/75 , G01N2333/976
摘要： The invention relates to the determination of the nature and strength of enzymatic activity in blood using mass spectrometric measurement of a profile of the reaction products. The determination of the enzymatic activity can be used for medical diagnostics, for example, and also to check the effectiveness of medication. The invention provides a method whereby adding probe substances usually not present in blood offers standardized substrates for measuring the enzymatic activity. The probe substances may be added to whole blood, plasma, or serum. The mass spectrometric measurement of the reaction products, after their reversible immobilization on actively binding surfaces of solids, for example, can deliver biomarker patterns of the reaction products which may be indicators for metabolic anomalies or diseases, since these are often accompanied by the formation or activation of characteristic enzymes.
摘要翻译： 本发明涉及使用反应产物分布的质谱测量来测定血液中酶活性的性质和强度。 酶活性的测定可以用于医学诊断，例如，以及检查药物的有效性。 本发明提供了通过添加通常不存在于血液中的探针物质来提供用于测量酶活性的标准化底物的方法。 探针物质可以添加到全血，血浆或血清中。 反应产物在其可固定地固定在固体的活性结合表面上之后的质谱测量可以提供反应产物的生物标志物模式，其可以是代谢异常或疾病的指标，因为它们通常伴随着形成或 特征酶的活化。
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公开(公告)号：US08227748B2
公开(公告)日：2012-07-24
申请号：US12784473
申请日：2010-05-20
CPC分类号： H01J49/4295 , H01J49/0072 , H01J49/422
摘要： In a linear ion trap, ions with two polarities are confined radially via an RF potential between the rods comprising the trap. Axially, ions of at least one polarity are confined via DC potentials applied to the elements of the trap or electrodes at the ends of the trap whereas ions of the other polarity are axially confined by a combination of pseudopotentials and/or DC potentials.
摘要翻译： 在线性离子阱中，具有两个极性的离子通过包含捕获阱的棒之间的RF电位在径向上被限制。 轴向上，至少一个极性的离子通过施加到陷阱的元件上的DC电位而被限制，而另一极性的离子被赝势和/或DC电势的组合轴向限制。
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6.发明授权Method and apparatus for avoiding undesirable mass dispersion of ions in flight 有权用于避免飞行中离子不期望质量分散的方法和装置公开(公告)号：US08013290B2
公开(公告)日：2011-09-06
申请号：US11779452
申请日：2007-07-18
CPC分类号： H01J49/4265 , H01J49/004 , H01J49/062
摘要： In a mass spectrometer a target volume is filled with ions of different mass but substantially the same energy from a distant storage device by forming a plurality of spatially-limited ion swarms consisting of ions having the same mass. The ion swarms are ordered either by a mass-sequential extraction from the storage device or by rearranging the order of flight as the ions are in flight, so that swarms of different mass ions simultaneously enter the target volume despite having different flight velocities. A mass-sequential extraction in the order of decreasing mass can be achieved in one embodiment by decreasing a pseudopotential barrier at the storage device which causes the heavy ions to emerge first. In another embodiment, the ions can be rearranged in flight by applying a bunching potential. A second reverse bunching potential then restores the energy of the ions to their original values.
摘要翻译： 在质谱仪中，通过形成由具有相同质量的离子组成的多个空间有限的离子群，在目标体积中填充来自遥远存储装置的质量不同但基本相同的能量的离子。 离子群可以通过从存储装置进行质量顺序提取或通过在离子飞行中重新排列飞行顺序来排序，这样即使具有不同的飞行速度，不同质量离子的群也同时进入目标体积。 在一个实施例中，可以通过减少存储装置处的伪电位势垒，使重离子首先出现，以质量递减的顺序提取质量。 在另一个实施方案中，通过施加聚束电位，可以在飞行中重新排列离子。 第二个反向聚束电位然后将离子的能量恢复到其原始值。
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7.发明申请METHOD AND APPARATUS FOR AVOIDING UNDESIRABLE MASS DISPERSION OF IONS IN FLIGHT 有权避免飞行中不可分离的大量离子的方法和装置公开(公告)号：US20080156980A1
公开(公告)日：2008-07-03
申请号：US11779452
申请日：2007-07-18
IPC分类号： H01J49/00
CPC分类号： H01J49/4265 , H01J49/004 , H01J49/062
摘要： In a mass spectrometer a target volume is filled with ions of different mass but substantially the same energy from a distant storage device by forming a plurality of spatially-limited ion swarms consisting of ions having the same mass. The ion swarms are ordered either by a mass-sequential extraction from the storage device or by rearranging the order of flight as the ions are in flight, so that swarms of different mass ions simultaneously enter the target volume despite having different flight velocities. A mass-sequential extraction in the order of decreasing mass can be achieved in one embodiment by decreasing a pseudopotential barrier at the storage device which causes the heavy ions to emerge first. In another embodiment, the ions can be rearranged in flight by applying a bunching potential. A second reverse bunching potential then restores the energy of the ions to their original values.
摘要翻译： 在质谱仪中，通过形成由具有相同质量的离子组成的多个空间有限的离子群，在目标体积中填充来自遥远存储装置的质量不同但基本相同的能量的离子。 离子群可以通过从存储装置进行质量顺序提取或通过在离子飞行中重新排列飞行顺序来排序，这样即使具有不同的飞行速度，不同质量离子的群也同时进入目标体积。 在一个实施例中，可以通过减少存储装置处的伪电位势垒，使重离子首先出现，以质量递减的顺序提取质量。 在另一个实施方案中，通过施加聚束电位，可以在飞行中重新排列离子。 第二个反向聚束电位然后将离子的能量恢复到其原始值。
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8.发明申请EXTRACTION OF SUBSTANCES OF INTEREST FROM BLOOD FOR MASS SPECTROMETRIC ANALYSIS 审中-公开从血液中提取物质用于质谱分析公开(公告)号：US20070196813A1
公开(公告)日：2007-08-23
申请号：US11626518
申请日：2007-01-24
IPC分类号： C12Q1/00 , G01N33/567 , C12M3/00 , A61M1/06
CPC分类号： A61B5/150793 , A61B5/150022 , A61B5/150343 , A61B5/150351 , A61B5/150755 , A61B5/150786 , B01L2200/185 , B01L2300/069 , G01N1/405 , G01N33/6848
摘要： In a method for preparing a blood sample for subsequent processing in a mass spectrometry laboratory, substances of interest are extracted from whole blood, blood plasma or blood serum immediately after the blood sample has been taken from a subject. The extraction is carried out by means of reversible immobilization on a surface, for example, the surface of a blood extraction vessel. Only the extracted substances of interest are sent in the immobilized state to the mass spectrometry laboratory.
摘要翻译： 在用于制备用于随后在质谱实验室中处理的血液样品的方法中，在从受试者取出血液样本后，立即从全血，血浆或血清中提取目的物质。 通过可逆地固定在表面上，例如血液提取容器的表面进行提取。 将所提取的目的物质以固定状态送入质谱实验室。
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公开(公告)号：US07737398B2
公开(公告)日：2010-06-15
申请号：US11955581
申请日：2007-12-13
申请人： Karsten Michelmann
发明人： Karsten Michelmann
CPC分类号： H01J49/025 , H01J49/423
摘要： In a linear ion trap in which an essentially quadrupole RF electrical field is generated between at least four rod-shaped electrodes, ions may be mass-selectively ejected orthogonally to the axis. An aspect of the invention comprises compensating for field irregularities along the axis of a linear ion trap, which result, at different ejection locations, in the ejection of ions of the same masses at slightly different times, by of measuring the ions that are ejected at the different ejection locations using a number of separate detectors, and correcting, after a mass calibration of each of the mass spectra, the time shifts of the various location-dependent mass spectra during their addition to a combined spectrum.
摘要翻译： 在其中在至少四个棒状电极之间产生基本上四极杆RF电场的线性离子阱中，离子可以被质量选择性地垂直于该轴线喷射。 本发明的一个方面包括补偿沿着线性离子阱的轴的场不规则，其导致在不同的喷射位置，在稍微不同的时间喷射同一质量的离子，通过测量在 使用多个单独的检测器的不同的喷射位置，并且在每个质谱的质量校准之后校正在添加到组合光谱期间各种位置依赖质谱的时间偏移。
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公开(公告)号：US20090084949A1
公开(公告)日：2009-04-02
申请号：US12037506
申请日：2008-02-26
申请人： Jochen Franzen , Karsten Michelmann
发明人： Jochen Franzen , Karsten Michelmann
IPC分类号： B01D59/44
CPC分类号： H01J49/42 , H01J49/0036 , H01J49/027
摘要： The invention relates to mass spectrometers in which ion clouds are stored in two spatial directions by radial forces while oscillating largely harmonically at a mass-specific frequency in a third spatial direction perpendicular to the other two, in a potential minimum, the shape of which is as close to a parabola as possible. Analysis of the oscillation frequencies of these ion clouds, preferably by a Fourier analysis, leads via a frequency spectrum to a mass spectrum. The frequency spectrum is analyzed to identify false signals in the frequency spectrum as harmonics and eliminating them where necessary.
摘要翻译： 本发明涉及质谱仪，其中离子云通过径向力在两个空间方向上存储，同时以与另外两个垂直的第三空间方向上的质量特定频率大量谐波地振荡，其形状为 尽可能接近抛物线。 这些离子云的振荡频率的分析优选通过傅立叶分析，经由频谱到质谱。 分析频谱以将频谱中的伪信号作为谐波识别，并在必要时消除它们。
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