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公开(公告)号：US20130273569A1
公开(公告)日：2013-10-17
申请号：US13777910
申请日：2013-02-26
发明人： Gayle BULLER , Jixiang LIU , Stephen YUE , Jolene BRADFORD
IPC分类号： C07D417/04 , C07D409/04 , C07D413/14 , C07D413/04 , G01N21/64
CPC分类号： C09B23/0075 , C07D409/04 , C07D413/04 , C07D413/14 , C07D417/04 , G01N21/6486 , G01N33/52 , G01N33/582 , G01N2021/6441
摘要： The present invention provides dye compounds optimally excited at about 400 nm and have a Stokes shift of at least about 80 nm. These dyes find use in detection of analyte in a sample and the preparation of dye-conjugates.
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公开(公告)号：US20150168383A1
公开(公告)日：2015-06-18
申请号：US14632168
申请日：2015-02-26
发明人： Stephen YUE , Shih-Jung HUANG , Jolene BRADFORD
CPC分类号： G01N33/52 , C07C225/36 , C07C237/10 , C07C2603/24 , C07D205/04 , C07D213/36 , C07D233/61 , C07D249/04 , C07D295/13 , C07D295/192 , C07H21/04 , C09B1/5165 , C12Q1/6816 , C12Q1/6841 , G01N21/6428 , G01N2021/6441
摘要： Disclosed are near IR emitting fluorescent compounds; methods of making and kits containing the described compounds; and their use in fluorescence-based detection of biological materials.
摘要翻译： 近红外发射荧光化合物; 制备方法和含有所述化合物的试剂盒; 以及它们在荧光检测生物材料中的应用。
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3.发明申请公开(公告)号：US20150004597A1
公开(公告)日：2015-01-01
申请号：US14488751
申请日：2014-09-17
IPC分类号： C12Q1/68 , C07D417/06
CPC分类号： G01N33/582 , C07D215/10 , C07D215/227 , C07D277/72 , C07D277/74 , C07D417/04 , C07D417/06 , C07D417/14 , C12Q1/6876 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G01N2201/06113 , Y10T436/143333
摘要： Chemical compounds having a high selectivity for double stranded DNA over RNA and single stranded DNA are disclosed. The chemical compounds are stains that become fluorescent upon illumination and interaction with double stranded DNA, but exhibit reduced or no fluorescence in the absence of double stranded DNA. The compounds can be used in a variety of biological applications to qualitatively or quantitatively assay DNA, even in the presence of RNA.
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公开(公告)号：US20160130637A1
公开(公告)日：2016-05-12
申请号：US14300728
申请日：2014-06-10
发明人： Scott CLARKE , Jolene BRADFORD
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q1/6804 , C12Q1/6846 , C12Q2565/1025 , C12Q2525/101
摘要： The present invention provides a method for measuring cellular nascent nucleic acid synthesis by dual pulse labeling of nucleic acid. The first pulse labeling of nucleic acid with a nucleoside analog allows establishment of a baseline nucleic acid synthesis rate. Pulse labeling of the nucleic acid with a second nucleoside analog then allows measurement of any changes to nucleic acid synthesis. The nucleic acid synthesis can be measured as cell proliferation, DNA, or gene expression, RNA. This method does not require a potentially artifact-inducing intermediary wash step between pulse labels. Additionally, this method may be used to screen compounds for their affect on cellular proliferation by treating cells or an organism with the test compound simultaneous to or before treatment with a competitive nucleoside analog.
摘要翻译： 本发明提供了一种通过核酸双重脉冲标记来测量细胞新生核酸合成的方法。 具有核苷类似物的核酸的第一次脉冲标记允许建立基线核酸合成速率。 用第二核苷类似物脉冲标记核酸，然后允许测量核酸合成的任何变化。 核酸合成可以作为细胞增殖，DNA或基因表达，RNA来测量。 该方法不需要在脉冲标签之间产生潜在的伪影中介洗涤步骤。 此外，该方法可用于通过用竞争性核苷类似物在治疗前或之前用受试化合物处理细胞或生物体来筛选化合物对细胞增殖的影响。
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公开(公告)号：US20150050685A1
公开(公告)日：2015-02-19
申请号：US14444023
申请日：2014-07-28
发明人： Gayle BULLER , Jixiang LIU , Stephen YUE , Jolene BRADFORD
CPC分类号： C09B23/0075 , C07D409/04 , C07D413/04 , C07D413/14 , C07D417/04 , G01N21/6486 , G01N33/52 , G01N33/582 , G01N2021/6441
摘要： The present invention provides dye compounds optimally excited at about 400 nm and have a Stokes shift of at least about 80 nm. These dyes find use in detection of analyte in a sample and the preparation of dye-conjugates.
摘要翻译： 本发明提供了在约400nm处最佳激发并具有至少约80nm的斯托克斯位移的染料化合物。 这些染料可用于检测样品中的分析物和染料缀合物的制备。
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公开(公告)号：US20130224735A1
公开(公告)日：2013-08-29
申请号：US13861123
申请日：2013-04-11
IPC分类号： G01N21/64
CPC分类号： G01N33/582 , C07D215/10 , C07D215/227 , C07D277/72 , C07D277/74 , C07D417/04 , C07D417/06 , C07D417/14 , C12Q1/6876 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G01N2201/06113 , Y10T436/143333
摘要： Chemical compounds having a high selectivity for double stranded DNA over RNA and single stranded DNA are disclosed. The chemical compounds are stains that become fluorescent upon illumination and interaction with double stranded DNA, but exhibit reduced or no fluorescence in the absence of double stranded DNA. The compounds can be used in a variety of biological applications to qualitatively or quantitatively assay DNA, even in the presence of RNA.
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7.发明申请公开(公告)号：US20180067044A1
公开(公告)日：2018-03-08
申请号：US15643019
申请日：2017-07-06
发明人： Gregory KADUCHAK , Michael WARD , Jolene BRADFORD , Andrew PARKER
CPC分类号： G01N21/453 , G01N15/1404 , G01N2015/142
摘要： A flow cytometer includes a capillary having a sample channel; at least one vibration producing transducer coupled to the capillary, the at least one vibration producing transducer being configured to produce an acoustic signal inducing acoustic radiation pressure within the sample channel to acoustically concentrate particles flowing within a fluid sample stream in the sample channel; and an interrogation source having a violet laser and a blue laser, the violet and blue lasers being configured to interact with at least some of the acoustically concentrated particles to produce an output signal.
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8.发明申请Fluorescent Chemical Compounds Having High Selectivity for Double Stranded DNA, and Methods for Their Use 有权对双链DNA具有高选择性的荧光化学化合物及其使用方法公开(公告)号：US20150316558A1
公开(公告)日：2015-11-05
申请号：US14797415
申请日：2015-07-13
IPC分类号： G01N33/58 , C07D417/06 , C07D417/14 , G01N21/64
CPC分类号： G01N33/582 , C07D215/10 , C07D215/227 , C07D277/72 , C07D277/74 , C07D417/04 , C07D417/06 , C07D417/14 , C12Q1/6876 , G01N21/6428 , G01N21/6486 , G01N2021/6439 , G01N2201/06113 , Y10T436/143333
摘要： Chemical compounds having a high selectivity for double stranded DNA over RNA and single stranded DNA are disclosed. The chemical compounds are stains that become fluorescent upon illumination and interaction with double stranded DNA, but exhibit reduced or no fluorescence in the absence of double stranded DNA. The compounds can be used in a variety of biological applications to qualitatively or quantitatively assay DNA, even in the presence of RNA.
摘要翻译： 公开了对RNA和单链DNA具有对双链DNA的高选择性的化合物。 化学化合物是在照射时变成荧光并与双链DNA相互作用的污渍，但在不存在双链DNA的情况下表现出减少的或没有荧光。 该化合物可用于各种生物学应用中，以定性或定量测定DNA，即使在RNA的存在下。
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