摘要:
A method for producing coenzyme Q10 by using stock culture from solid phase fermentation. The strain used in this method is Rhodobacter sphaeroides. Strain passaging is carried out by culturing in a slant medium. After steam cooking and air drying, solid medium is subpackaged and sterilized, wherein said solid medium includes solid components and liquid components. The fresh culture of Rhodobacter sphaeroides on the slant medium is added with sterile water, and the resultant bacterial suspension is added into the solid medium, cultured and used as stock culture for primary fermentation. The method of the present invention can enhance the fermentation level of coenzyme Q10, reduce the cascades of fermentation, shorten production cycle, simplify production processes, and lower production cost.
摘要:
A method of determining the cellular or genetic target of an antimicrobial compound includes cloning of a bacterial gene into an expression vector with an inducible promoter and determining whether increasing expression of the cloned gene in the cell will result in resistance to an antimicrobial compound. Also, the method can include incorporation of every gene of a bacterial strain into expression vectors having an inducible promoter, induction, treating with an antimicrobial compound, isolating the gene clone that confers cells resistant to the compound, and determining the identity of the resistant gene by various methods including DNA microarrays and gene sequencing.
摘要:
The invention provides birA polypeptides and polynucleotides encoding birA polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing birA polypeptides to screen for antibacterial compounds.
摘要:
The invention provides fabG polypeptides and polynucleotides encoding fabG polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing fabG polypeptides to screen for antibacterial compounds.
摘要:
A method for realizing route forwarding in a network, including: establishing LSPs in a bearer network; setting an entrance label in the starting node of each LSP pointing to forwarding information of the LSP; a bearer control entity assigning a transfer path for the service flow and sending the information of all corresponding entrance labels of the transfer path to the ingress edge node of the transfer path; when the service flow needs to be forwarded, the ingress edge node puts the label stack into the data packet of the service flow to generate an MPLS exchange packet; and forwarding the MPLS exchange packet in the bearer network according to the label stack until the packet reaches the egress edge node of the transfer path. The present invention grants high compatibility to the network, minimizes the network investment, and reduces the difficulties in network implementation.
摘要:
The invention provides MurA polypeptides and polynucleotides encoding MurA polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing MurA polypeptides to screen for antibacterial compounds.
摘要:
A system and method for detecting hand and item movement patterns comprising a video camera positioned to view a scene which includes therein a scanner for scanning items, wherein the video camera generates a sequence of video frames representing activity in the scene, processing means coupled to the video camera, the processing means performing steps of identifying regions of a video frame representing a hand; and tracking hand movement with respect to the scanner over a plurality of video frames. Event information descriptive of user activity at the self-service checkout terminal is generated based on the tracking information.
摘要:
An item recognition system and method which is particularly suited for automating entry of items too small to carry readable bar code labels. The system includes a camera which digitizes the image to produce a digitized image and a gray-scale digitized image. A binary image of the gray-scale image is then produced from which the computer records an image of the item, and a computer coupled to the camera which digitizes the image to produce a digitized image and a gray-scale digitized image. A binary image of the gray-scale image is then produced from which the computer identifies the item from the binary image and obtains the price from a price-lookup file.