METHOD FOR PRODUCING COENZYME Q10 BY FERMENTATION USING STOCK CULTURE FROM SOLID PHASE FERMENTATION
    1.
    发明申请
    METHOD FOR PRODUCING COENZYME Q10 BY FERMENTATION USING STOCK CULTURE FROM SOLID PHASE FERMENTATION 有权
    通过使用来自固相发酵的贮藏文化发酵生产聚苯乙烯Q10的方法

    公开(公告)号:US20130330789A1

    公开(公告)日:2013-12-12

    申请号:US14002199

    申请日:2011-04-26

    IPC分类号: C12P7/66

    CPC分类号: C12P7/66 C12N1/20 C12R1/01

    摘要: A method for producing coenzyme Q10 by using stock culture from solid phase fermentation. The strain used in this method is Rhodobacter sphaeroides. Strain passaging is carried out by culturing in a slant medium. After steam cooking and air drying, solid medium is subpackaged and sterilized, wherein said solid medium includes solid components and liquid components. The fresh culture of Rhodobacter sphaeroides on the slant medium is added with sterile water, and the resultant bacterial suspension is added into the solid medium, cultured and used as stock culture for primary fermentation. The method of the present invention can enhance the fermentation level of coenzyme Q10, reduce the cascades of fermentation, shorten production cycle, simplify production processes, and lower production cost.

    摘要翻译: 通过使用固相发酵的原料培养生产辅酶Q10的方法。 该方法中使用的菌株是球形红细菌。 菌株传代是通过在倾斜培养基中培养进行的。 在蒸汽烹饪和空气干燥之后,将固体培养基分包并灭菌,其中所述固体培养基包括固体组分和液体组分。 向斜面培养基上添加无菌水球状红细菌的新鲜培养物,将生成的细菌悬浮液加入到固体培养基中,培养并用作初级发酵的培养基。 本发明的方法可以提高辅酶Q10的发酵水平,降低发酵级联,缩短生产周期,简化生产工艺,降低生产成本。

    Identification of Targets of Antimicrobial Compounds
    2.
    发明申请
    Identification of Targets of Antimicrobial Compounds 审中-公开
    鉴定抗菌化合物的目标

    公开(公告)号:US20060240505A1

    公开(公告)日:2006-10-26

    申请号:US11427043

    申请日:2006-06-28

    IPC分类号: C12Q1/18 C12N1/21 C12N15/74

    摘要: A method of determining the cellular or genetic target of an antimicrobial compound includes cloning of a bacterial gene into an expression vector with an inducible promoter and determining whether increasing expression of the cloned gene in the cell will result in resistance to an antimicrobial compound. Also, the method can include incorporation of every gene of a bacterial strain into expression vectors having an inducible promoter, induction, treating with an antimicrobial compound, isolating the gene clone that confers cells resistant to the compound, and determining the identity of the resistant gene by various methods including DNA microarrays and gene sequencing.

    摘要翻译: 确定抗微生物化合物的细胞或遗传靶标的方法包括用诱导型启动子将细菌基因克隆到表达载体中,并确定克隆基因在细胞中的表达增加是否会导致对抗微生物化合物的抗性。 此外,该方法可以包括将细菌菌株的每个基因并入具有诱导型启动子的表达载体,诱导,用抗微生物化合物处理,分离赋予细胞对该化合物有抗性的基因克隆,以及确定抗性基因的同一性 通过各种方法,包括DNA微阵列和基因测序。

    Method for realizing route forwarding in network
    6.
    发明申请
    Method for realizing route forwarding in network 有权
    实现网络中路由转发的方法

    公开(公告)号:US20070133568A1

    公开(公告)日:2007-06-14

    申请号:US11634941

    申请日:2006-12-07

    IPC分类号: H04L12/56

    CPC分类号: H04L45/00 H04L12/66 H04L45/50

    摘要: A method for realizing route forwarding in a network, including: establishing LSPs in a bearer network; setting an entrance label in the starting node of each LSP pointing to forwarding information of the LSP; a bearer control entity assigning a transfer path for the service flow and sending the information of all corresponding entrance labels of the transfer path to the ingress edge node of the transfer path; when the service flow needs to be forwarded, the ingress edge node puts the label stack into the data packet of the service flow to generate an MPLS exchange packet; and forwarding the MPLS exchange packet in the bearer network according to the label stack until the packet reaches the egress edge node of the transfer path. The present invention grants high compatibility to the network, minimizes the network investment, and reduces the difficulties in network implementation.

    摘要翻译: 一种实现网络中路由转发的方法,包括:在承载网中建立LSP; 在每个LSP的起始节点上设置指向LSP转发信息的入口标签; 为所述服务流分配传送路径并向所述传送路径的所述入口边缘节点发送所述传送路径的所有对应入口标签的信息的承载控制实体; 当服务流需要转发时,入口边缘节点将标签栈放入业务流的数据包中,生成MPLS交换包; 并根据标签堆栈在承载网中转发MPLS交换报文,直到报文到达传输路径的出口边缘节点。 本发明赋予网络高兼容性,最小化网络投资,减少网络实现的难度。

    Price determination system and method using digitized gray-scale image
recognition and price-lookup files
    10.
    发明授权
    Price determination system and method using digitized gray-scale image recognition and price-lookup files 失效
    使用数字化灰度图像识别和价格查询文件的价格确定系统和方法

    公开(公告)号:US5969317A

    公开(公告)日:1999-10-19

    申请号:US748440

    申请日:1996-11-13

    摘要: An item recognition system and method which is particularly suited for automating entry of items too small to carry readable bar code labels. The system includes a camera which digitizes the image to produce a digitized image and a gray-scale digitized image. A binary image of the gray-scale image is then produced from which the computer records an image of the item, and a computer coupled to the camera which digitizes the image to produce a digitized image and a gray-scale digitized image. A binary image of the gray-scale image is then produced from which the computer identifies the item from the binary image and obtains the price from a price-lookup file.

    摘要翻译: 一种特别适用于自动进入太小的物品以携带可读条形码标签的物品识别系统和方法。 该系统包括将图像数字化以产生数字化图像和灰度数字化图像的相机。 然后生成灰度图像的二进制图像,计算机从该图像记录物品的图像,以及耦合到照相机的计算机,数字化图像以产生数字化图像和灰度数字化图像。 然后产生灰度图像的二进制图像,计算机从该图像识别来自二进制图像的项目并从价格查找文件获得价格。