公开(公告)号：US5041555A

公开(公告)日：1991-08-20

申请号：US585450

申请日：1990-09-21

申请人： George W. J. Fleet ,  Bryan Winchester ,  Neil M. Carpenter

发明人： George W. J. Fleet ,  Bryan Winchester ,  Neil M. Carpenter

IPC分类号： C07D207/12 ,  C07D317/28 ,  C07D407/04 ,  C07D493/04

CPC分类号： C07D407/04 ,  C07D207/12 ,  C07D317/28 ,  C07D493/04 ,  Y02P20/582

摘要： Novel derivatives of 1,4-dideoxy-1,4-imino-D-mannitol and method for their synthesis from 4,5-anhydro-1-azido-1-deoxy-2,3-O-isopropylidene-D-talitol or its triflate derivative are disclosed. The novel derivatives, 6-fluoro-1,4-imino-1,4,6-trideoxy-D-mannitol and the N-butyl and N-benzyl derivatives of 1,4-dideoxy-1,4-imino-D-mannitol, have useful mannosidase inhibitory activity.

摘要翻译： 1,4-二脱氧-1,4-亚氨基-D-甘露糖醇的新型衍生物及其由4,5-脱水-1-叠氮基-1-脱氧-2,3-O-异丙叉基-D-木糖醇或其合成方法 公开了其三氟甲磺酸酯衍生物。 新衍生物，6-氟-1,4-亚氨基-1,4,6-三脱氧-D-甘露糖醇和1,4-二脱氧-1,4-亚氨基-D-甘露醇的N-丁基和N-苄基衍生物， 甘露醇，具有有用的甘露糖苷酶抑制活性。

公开(公告)号：US4996329A

公开(公告)日：1991-02-26

申请号：US461859

申请日：1990-01-08

申请人： George W. J. Fleet ,  Bryan Winchester ,  Neil M. Carpenter

发明人： George W. J. Fleet ,  Bryan Winchester ,  Neil M. Carpenter

IPC分类号： A61K31/00 ,  A61K31/40 ,  A61P31/00 ,  A61P31/12 ,  A61P43/00 ,  C07D207/12 ,  C07D317/28 ,  C07D407/04 ,  C07D491/056 ,  C07D493/04

CPC分类号： C07D407/04 ,  C07D207/12 ,  C07D317/28 ,  C07D493/04 ,  Y02P20/582

摘要： Novel derivatives of 1,4-dideoxy-1,4-imino-D-mannitol and method for their synthesis from 4,5-anhydro-1-azido-1-deoxy-2,3-O-isopropylidene-D-talitol or its triflate derivative are disclosed. The novel derivatives, 6-fluoro-1,4-imino-1,4,6-trideoxy-D-mannitol and the N-butyl and N-benzyl derivatives of 1,4-dideoxy-1,4-amino-D-mannitol, have useful mannosidase inhibitory activity.

公开(公告)号：US20050142590A1

公开(公告)日：2005-06-30

申请号：US10973189

申请日：2004-10-26

申请人： Peter Meikle ,  John Hopwood ,  Bryan Winchester

发明人： Peter Meikle ,  John Hopwood ,  Bryan Winchester

IPC分类号： C12Q1/34 ,  C12Q1/68 ,  G01N33/573 ,  G01N33/68 ,  G01N33/53 ,  G01N33/537 ,  G01N33/543

CPC分类号： G01N33/573 ,  C12Q1/34 ,  C12Q1/6883 ,  C12Q2600/158 ,  G01N33/6893 ,  G01N2333/475 ,  G01N2333/70596 ,  G01N2333/924

摘要： Methods for assaying a lysosomal enzyme activity present in a blood sample obtained from a patient. The method combines the blood or plasma sample in a buffer with at least one binding reagent capable of reacting with alpha-glucosidase present in the blood sample to form an enzyme reagent complex. The lysosomal enzyme activity present in the blood sample is then determined from the enzyme reagent complex formed and compared to a mean level of alpha-glucosidase in a control population of individuals not having a lysosomal storage disease.

摘要翻译： 用于测定从患者获得的血液样品中存在的溶酶体酶活性的方法。 该方法将缓冲液中的血液或血浆样品与能够与血液样品中存在的α-葡糖苷酶反应的至少一种结合试剂结合形成酶试剂复合物。 然后从所形成的酶试剂复合物中确定存在于血液样品中的溶酶体酶活性，并与不具有溶酶体贮积病的个体的对照群体中的α-葡萄糖苷酶的平均水平进行比较。

公开(公告)号：US20050048567A1

公开(公告)日：2005-03-03

申请号：US10497218

申请日：2002-11-27

申请人： Bryan Winchester ,  Kevin Mills

发明人： Bryan Winchester ,  Kevin Mills

IPC分类号： G01N27/62 ,  G01N33/483 ,  G01N33/58 ,  G01N33/92 ,  G01N33/53 ,  G01N33/20

CPC分类号： G01N33/92 ,  G01N33/58 ,  G01N2458/15 ,  G01N2496/00

摘要： The present invention relates to an internal standard for use in mass spectometry to determine the concentration of a test sphingolipid, the internal standard comprising the same oligosaccharyl chain and long chain base sphingosine as the test sphingolipid and having an acyl group of different mass from the test sphingolipid but providing a sphingolipid of the same chemical nature as the test sphingolipid, and methods for the preparation and use thereof.

摘要翻译： 本发明涉及用于确定测试鞘脂浓度的质谱法的内标，内标包含与测试鞘脂相同的寡糖基链和长链基鞘氨醇，并具有与测试不同质量的酰基 鞘脂，但提供与测试鞘脂具有相同化学性质的鞘脂，以及其制备和使用方法。