公开(公告)号：US20110243389A1

公开(公告)日：2011-10-06

申请号：US13164123

申请日：2011-06-20

Applicant: Ron KNOX ,  Karl Boettger ,  Peter Meikle ,  Brian Alexander

Inventor： Ron KNOX ,  Karl Boettger ,  Peter Meikle ,  Brian Alexander

IPC: G06K9/00

CPC classification number: G01N21/53 ,  G01N15/1434 ,  G01N21/84 ,  G01N21/85 ,  G01N2201/06113 ,  G01N2201/12 ,  G01S7/4802 ,  G01S7/4811 ,  G01S7/497 ,  G01S17/89 ,  G06K9/46 ,  G08B17/10 ,  G08B17/103 ,  G08B17/107 ,  G08B17/125

Abstract: A smoke detecting method which uses a beam of radiation such as a laser (16), to monitor a region, such as a room (12). A camera (14) is used to capture images of part of the room (12), including a path of the laser beam. Particles in the laser beam scatter light (30), and this is captured by the camera (14) for analysis. A processor (20) extracts data relating to the scattered light (30) to determine the density of particles in the beam, to determine the level of smoke in the region. The laser may have a modulated output (38) so that images captured without the laser tuned “on” can be used as a reference point and compared to images taken with the laser turned “on”, to assist in determining the level of scattered light (30) compared to ambient light. Filters (24, 26) may be used to decrease signals generated from background light.

Abstract translation: 使用诸如激光（16）的辐射束的烟雾检测方法来监视诸如房间（12）的区域。 相机（14）用于捕获包括激光束的路径的房间（12）的一部分的图像。 激光束中的粒子散射光（30），并且由相机（14）捕获用于分析。 处理器（20）提取与散射光（30）相关的数据，以确定光束中的粒子的密度，以确定该区域中的烟雾水平。 激光器可以具有调制输出（38），使得在没有激光调谐为“开”的情况下拍摄的图像可以用作参考点，并且与激光器“打开”拍摄的图像进行比较，以帮助确定散射光的水平 （30）与环境光相比。 滤波器（24,26）可用于减少从背景光产生的信号。

公开(公告)号：US20060286034A1

公开(公告)日：2006-12-21

申请号：US10517899

申请日：2003-06-13

Applicant: Peter Meikle ,  Maria Fuller ,  Steven Ramsey ,  Enzo Ranieri ,  John Hopwood

Inventor： Peter Meikle ,  Maria Fuller ,  Steven Ramsey ,  Enzo Ranieri ,  John Hopwood

IPC: A61K49/00 ,  G01N33/53 ,  G01N33/00

CPC classification number: G01N33/66 ,  G01N2400/40

Abstract: The present invention is related to methods for diagnosing mucopolysaccharidoses (“MPS”) and related diseases. This invention pertains to methods for identifying and quantitating biochemical markers (“biomarkers”) that are present in biological fluids or tissues of a patient having a MPS or related disorder. One aspect of the method comprises determining a target quantity of a target MPS biomarker oligosaccharide from a target biological sample taken from the target animal, and then comparing the target quantity to a reference quantity of a reference MPS biomarker oligosaccharide for the diagnosis, characterization, monitoring, and clinical management of MPS and related disease. This invention also describes a kit comprising a oligosaccharide derivatization solution; an acid solution; an internal standard; a solid phase extraction column; a solid phase extraction column wash solution; an oligosaccharide elution solution; and a set of instructions for using the kit to diagnose a MPS or related disease.

Abstract translation: 本发明涉及用于诊断粘多糖贮积症（“MPS”）和相关疾病的方法。 本发明涉及用于鉴定和定量存在于具有MPS或相关病症的患者的生物流体或组织中的生化标志物（“生物标志物”）的方法。 该方法的一个方面包括从取自目标动物的目标生物样品中确定目标MPS生物标记寡糖的目标量，然后将目标量与参考MPS生物标记寡糖的参考量进行比较，以进行诊断，表征，监测 ，以及MPS及相关疾病的临床管理。 本发明还描述了一种包含寡糖衍生化溶液的试剂盒; 酸溶液; 内部标准; 固相萃取柱; 固相萃取柱洗涤液; 寡糖洗脱液; 以及使用该套件诊断MPS或相关疾病的一组说明。

公开(公告)号：US08406471B2

公开(公告)日：2013-03-26

申请号：US13164123

申请日：2011-06-20

Applicant: Ron Knox ,  Karl Boettger ,  Peter Meikle ,  Brian Alexander

Inventor： Ron Knox ,  Karl Boettger ,  Peter Meikle ,  Brian Alexander

IPC: G06K9/00

CPC classification number: G01N21/53 ,  G01N15/1434 ,  G01N21/84 ,  G01N21/85 ,  G01N2201/06113 ,  G01N2201/12 ,  G01S7/4802 ,  G01S7/4811 ,  G01S7/497 ,  G01S17/89 ,  G06K9/46 ,  G08B17/10 ,  G08B17/103 ,  G08B17/107 ,  G08B17/125

Abstract: A smoke detecting method which uses a beam of radiation such as a laser (16), to monitor a region, such as a room (12). A camera (14) is used to capture images of part of the room (12), including a path of the laser beam. Particles in the laser beam scatter light (30), and this is captured by the camera (14) for analysis. A processor (20) extracts data relating to the scattered light (30) to determine the density of particles in the beam, to determine the level of smoke in the region. The laser may have a modulated output (38) so that images captured without the laser tuned “on” can be used as a reference point and compared to images taken with the laser turned “on”, to assist in determining the level of scattered light (30) compared to ambient light. Filters (24, 26) may be used to decrease signals generated from background light.

Abstract translation: 使用诸如激光（16）的辐射束的烟雾检测方法来监视诸如房间（12）的区域。 相机（14）用于捕获包括激光束的路径的房间（12）的一部分的图像。 激光束中的粒子散射光（30），并且由相机（14）捕获用于分析。 处理器（20）提取与散射光（30）相关的数据，以确定光束中的粒子的密度，以确定该区域中的烟雾水平。 激光器可以具有调制输出（38），使得在没有激光器调谐的情况下拍摄的图像可以用作参考点，并且与激光打开的图像进行比较，以帮助确定比较的散射光的水平（30） 到环境光。 滤波器（24,26）可用于减少从背景光产生的信号。

公开(公告)号：US20070072243A1

公开(公告)日：2007-03-29

申请号：US10551396

申请日：2004-03-31

Applicant: Peter Meikle ,  John Hopwood ,  Douglas Brooks ,  Caroline Dean

Inventor： Peter Meikle ,  John Hopwood ,  Douglas Brooks ,  Caroline Dean

IPC: G01N33/567 ,  G01N33/53

CPC classification number: G01N33/543 ,  A61K49/0058 ,  A61K49/0091 ,  G01N33/54313 ,  G01N33/582 ,  G01N33/6893

Abstract: A novel protein profiling method of testing for Lysosomal Storage Diseases (“LSD”) using discovered normalized lysosomal fingerprint patterns. The fingerprint patterns reveal the health of lysosomal organelles, specific LSD, and clinical severity Multiplexing bead technology for simultaneous screening of multiple LSD and normalizing measured enzyme activity or protein levels against other lysosomal proteins, enzymes, or enzyme activities. Compounds, reagents, and methods for identifying and quantifying multiple target enzymes and proteins.

Abstract translation: 一种使用发现的归一化溶酶体指纹图谱测定溶酶体贮积病（“LSD”）的新型蛋白质分析方法。 指纹图谱揭示了溶酶体细胞器，特异性LSD和临床严重性的复合珠粒技术，用于同时筛选多种LSD并使测定的酶活性或蛋白质水平与其他溶酶体蛋白质，酶或酶活性正常化。 用于鉴定和定量多种靶酶和蛋白质的化合物，试剂和方法。

公开(公告)号：US20080233655A1

公开(公告)日：2008-09-25

申请号：US10594699

申请日：2005-03-31

Applicant: Peter Meikle ,  John Hopwood ,  Maria Fuller ,  Phillip Whitfield ,  Peter Sharp

Inventor： Peter Meikle ,  John Hopwood ,  Maria Fuller ,  Phillip Whitfield ,  Peter Sharp

IPC: G01N33/00

CPC classification number: G01N33/92

Abstract: A method of ascertaining the LSD (Lysosomal storage disorder) status of an individual comprising taking a tissue or body fluid sample from the individual and estimating a level in the sample of each of three or more compound indicators. The indicators reflect the level of respectively each of three or more lipid containing storage associated compounds. The levels are used to calculate an LSD index number which is then compared with a standard to provide an assessment of the LSD status of the individual. The indicator compounds are conveniently phospholipids, glycolipids or lipopolysaccharide species measured by mass spectrometry. The method may be used to ascertain the nature of the disorder from which the individual stuffers, and its severity. It may also be used to monitor the progress of treatment and to ascertain the prospects of an individual contracting an LSD by providing a subclinical indicators for the condition.

Abstract translation: 确定个体的LSD（溶酶体储存障碍）状态的方法，包括从个体取组织或体液样品并估计三个或更多个化合物指示剂中每一个的样品中的水平。 这些指标反映了三种或更多种与脂质相关的化合物中各自的水平。 水平用于计算LSD指数，然后将其与标准进行比较，以提供个体的LSD状态的评估。 指示剂化合物方便地通过质谱法测量磷脂，糖脂或脂多糖物质。 该方法可以用于确定个体填充物的病症的性质及其严重性。 它还可用于监测治疗进展情况，并通过为病情提供亚临床指标来确定个体承包LSD的前景。

公开(公告)号：US20070265432A1

公开(公告)日：2007-11-15

申请号：US11291621

申请日：2005-12-01

Applicant: Peter Meikle ,  John Hopwood ,  Douglas Brooks ,  Caroline Dean

Inventor： Peter Meikle ,  John Hopwood ,  Douglas Brooks ,  Caroline Dean

IPC: C07K16/18

CPC classification number: G01N33/54313 ,  C07K16/18 ,  C07K16/2896 ,  G01N2333/916 ,  G01N2333/924

Abstract: Multiplexing bead technology is used for simultaneous screening of multiple LSD and normalizing measured enzyme activity or protein levels against other lysosomal proteins, enzymes, or enzyme activities. Diagnostic compositions include microspheres conjugated to purified antibodies that specifically bind LSD target antigens: saposin, LAMP-1, α-iduronidase, α-glucosidase, β-glucosidase, 2-sulphatase, 4-sulphatase, α-galactosidase, sphingomyelinase, 3-sulphatase or sulphamidase. The target antigens are naturally present in biological fluids or tissues of either LSD or non-LSD patients.

Abstract translation: 多重珠粒技术用于同时筛选多种LSD，并将测定的酶活性或蛋白质水平与其他溶酶体蛋白质，酶或酶活性标准化。 诊断组合物包括与纯化抗体结合的微球，其特异性结合LSD靶抗原：皂角蛋白，LAMP-1，α-艾杜糖酶，α-葡糖苷酶，β-葡糖苷酶，2-硫酸酯酶，4-硫酸酯酶，α-半乳糖苷酶，鞘磷脂酶，3-硫酸酯酶 或磺酰胺酶。 目标抗原天然存在于LSD或非LSD患者的生物流体或组织中。

公开(公告)号：US07983445B2

公开(公告)日：2011-07-19

申请号：US10556807

申请日：2004-05-14

Applicant: Ron Knox ,  Karl Boettger ,  Peter Meikle ,  Brian Alexander

Inventor： Ron Knox ,  Karl Boettger ,  Peter Meikle ,  Brian Alexander

IPC: G06K9/00

CPC classification number: G01N21/53 ,  G01N15/1434 ,  G01N21/84 ,  G01N21/85 ,  G01N2201/06113 ,  G01N2201/12 ,  G01S7/4802 ,  G01S7/4811 ,  G01S7/497 ,  G01S17/89 ,  G06K9/46 ,  G08B17/10 ,  G08B17/103 ,  G08B17/107 ,  G08B17/125

Abstract: A smoke detecting method which uses a beam of radiation such as a laser (16), to monitor a region, such as a room (12). A camera (14) is used to capture images of part of the room (12), including a path of the laser beam. Particles in the laser beam scatter light (30), and this is captured by the camera (14) for analysis. A processor (20) extracts data relating to the scattered light (30) to determine the density of particles in the beam, to determine the level of smoke in the region. The laser may have a modulated output (38) so that images captured without the laser tuned “on” can be used as a reference point and compared to images taken with the laser turned “on”, to assist in determining the level of scattered light (30) compared to ambient light. Filters (24, 26) may be used to decrease signals generated from background light.

Abstract translation: 使用诸如激光（16）的辐射束的烟雾检测方法来监视诸如房间（12）的区域。 相机（14）用于捕获包括激光束的路径的房间（12）的一部分的图像。 激光束中的粒子散射光（30），并且由相机（14）捕获用于分析。 处理器（20）提取与散射光（30）相关的数据，以确定光束中的粒子的密度，以确定该区域中的烟雾水平。 激光器可以具有调制输出（38），使得在没有激光调谐为“开”的情况下拍摄的图像可以用作参考点，并且与激光器“打开”拍摄的图像进行比较，以帮助确定散射光的水平 （30）与环境光相比。 滤波器（24,26）可用于减少从背景光产生的信号。

公开(公告)号：US20070064980A1

公开(公告)日：2007-03-22

申请号：US10556807

申请日：2004-05-14

Applicant: Ron Knox ,  Karl Boettger ,  Peter Meikle ,  Brian Alexander

Inventor： Ron Knox ,  Karl Boettger ,  Peter Meikle ,  Brian Alexander

IPC: G06T7/20

CPC classification number: G01N21/53 ,  G01N15/1434 ,  G01N21/84 ,  G01N21/85 ,  G01N2201/06113 ,  G01N2201/12 ,  G01S7/4802 ,  G01S7/4811 ,  G01S7/497 ,  G01S17/89 ,  G06K9/46 ,  G08B17/10 ,  G08B17/103 ,  G08B17/107 ,  G08B17/125

Abstract: A smoke detector (10) is disclosed which uses a beam of radiation such as a laser (16), to monitor a region, such a room (12). A camera (14) is used to capture images of part of the room (12), including a path of the laser beam. Particles in the laser beam scatter light (30), and this is captured by the camera (14) for analysis. A processor (20) extracts data relating to the scattered light (30) to determine the density of particles in the beam, to determine the level of smoke in the region. The laser may have a modulated output (38) so that images captured without the laser can be used as a reference point and compared to images taken with the laser on, to assist in determining the level of scattered light (30) compared to ambient light. Filters (24, 26) may be used to decrease signals generated from background light.

Abstract translation: 公开了一种烟雾探测器（10），其使用诸如激光（16）的辐射束来监测区域，例如房间（12）。 相机（14）用于捕获包括激光束的路径的房间（12）的一部分的图像。 激光束中的粒子散射光（30），并且由相机（14）捕获用于分析。 处理器（20）提取与散射光（30）相关的数据，以确定光束中的粒子的密度，以确定该区域中的烟雾水平。 激光器可以具有调制输出（38），使得不用激光捕获的图像可以用作参考点并与用激光打开的图像进行比较，以帮助确定与环境光相比的散射光（30）的水平 。 滤波器（24,26）可用于减少从背景光产生的信号。

公开(公告)号：US20050142590A1

公开(公告)日：2005-06-30

申请号：US10973189

申请日：2004-10-26

Applicant: Peter Meikle ,  John Hopwood ,  Bryan Winchester

Inventor： Peter Meikle ,  John Hopwood ,  Bryan Winchester

IPC: C12Q1/34 ,  C12Q1/68 ,  G01N33/573 ,  G01N33/68 ,  G01N33/53 ,  G01N33/537 ,  G01N33/543

CPC classification number: G01N33/573 ,  C12Q1/34 ,  C12Q1/6883 ,  C12Q2600/158 ,  G01N33/6893 ,  G01N2333/475 ,  G01N2333/70596 ,  G01N2333/924

Abstract: Methods for assaying a lysosomal enzyme activity present in a blood sample obtained from a patient. The method combines the blood or plasma sample in a buffer with at least one binding reagent capable of reacting with alpha-glucosidase present in the blood sample to form an enzyme reagent complex. The lysosomal enzyme activity present in the blood sample is then determined from the enzyme reagent complex formed and compared to a mean level of alpha-glucosidase in a control population of individuals not having a lysosomal storage disease.

Abstract translation: 用于测定从患者获得的血液样品中存在的溶酶体酶活性的方法。 该方法将缓冲液中的血液或血浆样品与能够与血液样品中存在的α-葡糖苷酶反应的至少一种结合试剂结合形成酶试剂复合物。 然后从所形成的酶试剂复合物中确定存在于血液样品中的溶酶体酶活性，并与不具有溶酶体贮积病的个体的对照群体中的α-葡萄糖苷酶的平均水平进行比较。