知搜-全球专利检索

  1. 登录
  2. 注册

搜索结果

13 条记录 (耗时 0.016 秒)

    Devices and methods useful for detecting mechanical forces of ligand receptor interactions
    1.
    发明授权
    Devices and methods useful for detecting mechanical forces of ligand receptor interactions 有权

    公开(公告)号：US10900069B2

    公开(公告)日：2021-01-26

    申请号：US15456024

    申请日：2017-03-10

    申请人： Emory University

    发明人： Khalid Salaita ,  Pui-yan Ma

    IPC分类号： C12P19/34 ,  C12Q1/6816 ,  C12Q1/6848 ,  C12Q1/6809 ,  C12Q1/6844 ,  C12Q1/28 ,  G01N33/542

    摘要： This disclosure related to methods of detecting mechanical forces required to separate ligand and receptor interactions. In certain embodiments, this disclosure relates to methods of detecting mechanical forces between a ligand and receptor, where the ligand is immobilized on a surface using weaker forces. Ligand-receptor forces lead to dissociation of the ligand that can be detected and amplified. In certain embodiments, the disclosure relates to methods of detecting ligand and receptor interactions comprising linking a ligand to one of two binding partners, wherein the binding partners have attracting forces that are less than the forces between the ligand and a receptor of the ligand such that when the ligand binds the receptor, the binding partners will separate. Separation of the binding partners can be detected.

    Devices and Methods Useful for Imaging Transient and Rare Mechanical Events in Cells
    4.
    发明申请
    Devices and Methods Useful for Imaging Transient and Rare Mechanical Events in Cells 有权

    公开(公告)号：US20210024985A1

    公开(公告)日：2021-01-28

    申请号：US16913187

    申请日：2020-06-26

    申请人： Emory University

    发明人： Khalid Salaita ,  Rong Ma

    IPC分类号： C12Q1/6837 ,  C12Q1/6809 ,  C12Q1/6818 ,  G01N15/14

    摘要： In certain embodiments, this disclosure relates to devices and methods for imaging transient mechanical events in cells. In certain embodiments, this disclosure contemplates devices comprising receptors, cells or cell membranes comprising receptors, a molecular beacon as a linker between a solid surface and a ligand, and a locking oligonucleotide that selectively binds a portion of the hairpin turn and stem of the molecular beacon when the beacon is mechanically melted with piconewton forces. In certain embodiments, this disclosure relates to methods of locking, unlocking, and imaging cellular events using labeled locking and unlocking oligonucleotides disclosed herein.

    Polynucleotide Based Movement, Kits and Methods Related Thereto
    5.
    发明申请
    Polynucleotide Based Movement, Kits and Methods Related Thereto 审中-公开

    公开(公告)号：US20200332350A1

    公开(公告)日：2020-10-22

    申请号：US16922449

    申请日：2020-07-07

    申请人： Emory University

    发明人： Khalid Salaita ,  Kevin Yehl

    IPC分类号： C12Q1/6827 ,  C12N9/22 ,  C12Q1/6825

    摘要： This disclosure relates DNA based movement of objects. In certain embodiments, particles, pairs of particles, or rods are conjugated with single stranded DNA that hybridizes to a single stranded RNA that is conjugated to a substrate. When the DNA particle, pair of particles, or rod interacts with the surface RNA in the presence of an endonuclease, such as RNase H and the DNA hybridizes to the RNA, then the particle, pair of particles, or rod moves along the surface. The complementarity of the DNA and RNA affect the velocity.

    Polynucleotide based movement, kits and methods related thereto
    6.
    发明授权
    Polynucleotide based movement, kits and methods related thereto 有权

    公开(公告)号：US10738349B2

    公开(公告)日：2020-08-11

    申请号：US15332007

    申请日：2016-10-24

    申请人： Emory University

    发明人： Khalid Salaita ,  Kevin Yehl

    IPC分类号： C12Q1/68 ,  C12Q1/6827 ,  C12N9/22 ,  C12Q1/6825

    摘要： This disclosure relates DNA based movement of objects. In certain embodiments, particles, pairs of particles, or a rods are conjugated with single stranded DNA that hybridizes to a single stranded RNA that is conjugated to a substrate. When the DNA particle, pair of particles, or rod interacts with the surface RNA in the presence of an endonuclease, such as RNase H and the DNA hybridizes to the RNA, then the particle, pair of particle, or rod moves along the surface. The complementarity of the DNA and RNA affect the velocity.