公开(公告)号：US10161939B2

公开(公告)日：2018-12-25

申请号：US14765071

申请日：2013-05-31

申请人： Duke University ,  Janssen Diagnostics, LLC

发明人： Galla Chandra Rao ,  Mark C. Connelly ,  Mariano A. Garcia-Blanco ,  Andrew J. Armstrong ,  Rhonda L. Bitting

IPC分类号： G01N33/574 ,  G01N33/543 ,  G01N33/569

摘要： Provided are methods for detecting or isolating circulating tumor cells (CTCs) in a subject. The methods may include detecting the expression of at least one epithelial mesenchymal transition (EMT) biomarker. Further provided are kits for detecting or isolating CTCs. The kits may include antibodies to at least one EMT biomarker. Further provided are methods of predicting the responsiveness of a subject to a cancer drug, methods of targeting delivery of a cancer drug in a subject, methods of providing a cancer prognosis to a subject, and methods for following the progress of cancer in a subject.

公开(公告)号：US20180231560A1

公开(公告)日：2018-08-16

申请号：US15511229

申请日：2015-09-15

申请人： Duke University ,  JANSSEN DIAGNOSTICS, LLC

发明人： Galla Chandra Rao ,  Mark C. Connelly ,  Rengasamy BOOMINATHAN ,  Tian Zhang ,  Mariano A. Garcia-Blanco ,  Andrew J. Armstrong

IPC分类号： G01N33/574 ,  C07K16/30 ,  C07K16/28

CPC分类号： G01N33/57484 ,  C07K16/18 ,  C07K16/2863 ,  C07K16/289 ,  C07K16/30 ,  G01N33/57419 ,  G01N33/57438

摘要： This disclosure provides compositions and methods for the isolation of cells that express c-MET, and in particular circulating tumor cells that express c-MET. The methods can include contacting a biological sample including a c-MET circulating tumor cell with an unbound complex including a capture binding species linked to a solid phase for a time sufficient to allow the unbound complex to bind an extracellular binding domain of the c-MET protein to form a bound complex, and subsequently isolating the bound complex. Compositions, systems, and kits adapted for use with these methods are also provided.

公开(公告)号：US11567081B2

公开(公告)日：2023-01-31

申请号：US16206932

申请日：2018-11-30

申请人： Duke University

发明人： Galla Chandra Rao ,  Mark C. Connelly ,  Mariano A. Garcia-Blanco ,  Andrew J. Armstrong ,  Rhonda L. Bitting

IPC分类号： G01N33/574 ,  G01N33/543 ,  G01N33/569

摘要： Provided are methods for detecting or isolating circulating tumor cells (CTCs) in a subject. The methods may include detecting the expression of at least one epithelial mesenchymal transition (EMT) biomarker. Further provided are kits for detecting or isolating CTCs. The kits may include antibodies to at least one EMT biomarker. Further provided are methods of predicting the responsiveness of a subject to a cancer drug, methods of targeting delivery of a cancer drug in a subject, methods of providing a cancer prognosis to a subject, and methods for following the progress of cancer in a subject.

公开(公告)号：US20230184776A1

公开(公告)日：2023-06-15

申请号：US18063678

申请日：2022-12-08

申请人： Duke University ,  Menarini Silicon Biosystems S.p.A.

发明人： Galla Chandra Rao ,  Mark C. Connelly ,  Mariano A. Garcia-Blanco ,  Andrew J. Armstrong ,  Rhonda L. Bitting

IPC分类号： G01N33/574 ,  G01N33/543 ,  G01N33/569

CPC分类号： G01N33/57484 ,  G01N33/54326 ,  G01N33/56966 ,  G01N33/57415 ,  G01N33/57434 ,  G01N33/57492 ,  G01N2333/70589 ,  G01N2333/47 ,  G01N2333/4703 ,  G01N2333/4742 ,  G01N2333/705 ,  G01N2333/70503 ,  G01N2333/70596

摘要： Provided are methods for detecting or isolating circulating tumor cells (CTCs) in a subject. The methods may include detecting the expression of at least one epithelial mesenchymal transition (EMT) biomarker. Further provided are kits for detecting or isolating CTCs. The kits may include antibodies to at least one EMT biomarker. Further provided are methods of predicting the responsiveness of a subject to a cancer drug, methods of targeting delivery of a cancer drug in a subject, methods of providing a cancer prognosis to a subject, and methods for following the progress of cancer in a subject.

公开(公告)号：US20190107542A1

公开(公告)日：2019-04-11

申请号：US16206932

申请日：2018-11-30

申请人： Duke University

发明人： Galla Chandra Rao ,  Mark C. Connelly ,  Mariano A. Garcia-Blanco ,  Andrew J. Armstrong ,  Rhonda L. Bitting

IPC分类号： G01N33/574 ,  G01N33/569 ,  G01N33/543

摘要： Provided are methods for detecting or isolating circulating tumor cells (CTCs) in a subject. The methods may include detecting the expression of at least one epithelial mesenchymal transition (EMT) biomarker. Further provided are kits for detecting or isolating CTCs. The kits may include antibodies to at least one EMT biomarker. Further provided are methods of predicting the responsiveness of a subject to a cancer drug, methods of targeting delivery of a cancer drug in a subject, methods of providing a cancer prognosis to a subject, and methods for following the progress of cancer in a subject.

公开(公告)号：US09845481B2

公开(公告)日：2017-12-19

申请号：US15055880

申请日：2016-02-29

申请人： Duke University

发明人： Matthew S. Marengo ,  Mariano A. Garcia-Blanco

IPC分类号： C12P21/06 ,  C12N15/85 ,  C12N5/04 ,  C12N5/07 ,  C12N15/00 ,  C12N15/09 ,  C12N15/11 ,  C12N15/12 ,  C12N15/29 ,  C12N15/82

CPC分类号： C12N15/85 ,  C12N2800/30 ,  C12N2830/008 ,  C12N2840/007

摘要： Synthetic regulation of gene expression is provided. In some embodiments, synthetic regulatory constructs are provided. In some embodiments, a synthetic regulatory construct expresses a heterologous gene in a selected cell type. In some embodiments, methods of expressing a heterologous gene in a selected cell type are provided.

公开(公告)号：US20160281107A1

公开(公告)日：2016-09-29

申请号：US15055880

申请日：2016-02-29

申请人： Duke University

发明人： Matthew S. Marengo ,  Mariano A. Garcia-Blanco

IPC分类号： C12N15/85

CPC分类号： C12N15/85 ,  C12N2800/30 ,  C12N2830/008 ,  C12N2840/007

摘要： Synthetic regulation of gene expression is provided. In some embodiments, synthetic regulatory constructs are provided. In some embodiments, a synthetic regulatory construct expresses a heterologous gene in a selected cell type. In some embodiments, methods of expressing a heterologous gene in a selected cell type are provided.

公开(公告)号：US20160077097A1

公开(公告)日：2016-03-17

申请号：US14765071

申请日：2013-05-31

申请人： DUKE UNIVERSITY

发明人： Galla Chandra Rao ,  Mark C. Connelly ,  Mariano A. Garcia-Blanco ,  Andrew J. Armstrong ,  Rhonda L. Bitting

IPC分类号： G01N33/574

CPC分类号： G01N33/57484 ,  G01N33/54326 ,  G01N33/56966 ,  G01N33/57415 ,  G01N33/57434 ,  G01N33/57492 ,  G01N2333/47 ,  G01N2333/4703 ,  G01N2333/4742 ,  G01N2333/705 ,  G01N2333/70503 ,  G01N2333/70589 ,  G01N2333/70596

摘要： Provided are methods for detecting or isolating circulating tumor cells (CTCs) in a subject. The methods may include detecting the expression of at least one epithelial mesenchymal transition (EMT) biomarker. Further provided are kits for detecting or isolating CTCs. The kits may include antibodies to at least one EMT biomarker. Further provided are methods of predicting the responsiveness of a subject to a cancer drug, methods of targeting delivery of a cancer drug in a subject, methods of providing a cancer prognosis to a subject, and methods for following the progress of cancer in a subject.

摘要翻译： 提供了用于检测或分离受试者中的循环肿瘤细胞（CTC）的方法。 所述方法可以包括检测至少一种上皮间质转化（EMT）生物标志物的表达。 还提供了用于检测或隔离四氯化碳的试剂盒。 试剂盒可以包括至少一种EMT生物标志物的抗体。 进一步提供了预测受试者对癌症药物的反应性的方法，靶向受试者中癌症药物递送的方法，对受试者提供癌症预后的方法，以及用于跟踪受试者的癌症进展的方法。

公开(公告)号：US09284575B2

公开(公告)日：2016-03-15

申请号：US13783450

申请日：2013-03-04

申请人： Duke University

发明人： Matthew S. Marengo ,  Mariano A. Garcia-Blanco

IPC分类号： C12P21/06 ,  C12N15/85 ,  C12N5/04 ,  C12N5/07 ,  C12N15/00 ,  C12N15/09 ,  C12N15/11 ,  C12N15/12 ,  C12N15/29 ,  C12N15/82

CPC分类号： C12N15/85 ,  C12N2800/30 ,  C12N2830/008 ,  C12N2840/007

摘要： Synthetic regulation of gene expression is provided. In some embodiments, synthetic regulatory constructs are provided. In some embodiments, a synthetic regulatory construct expresses a heterologous gene in a selected cell type. In some embodiments, methods of expressing a heterologous gene in a selected cell type are provided.

摘要翻译： 提供基因表达的合成调节。 在一些实施方案中，提供合成的调节构建体。 在一些实施方案中，合成调节构建体表达所选细胞类型的异源基因。 在一些实施方案中，提供了以所选细胞类型表达异源基因的方法。