知搜-全球专利检索

  1. Login
  2. Sign Up

Search Results

32 records (took 0.069 seconds)

    SYSTEM AND METHOD FOR ISOLATING AND ANALYZING CELLS
    2.
    发明申请
    SYSTEM AND METHOD FOR ISOLATING AND ANALYZING CELLS 有权

    公开(公告)号：US20210283608A1

    公开(公告)日：2021-09-16

    申请号：US17333142

    申请日：2021-05-28

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Kalyan Handique ,  Vishal Sharma ,  Priyadarshini Gogoi ,  William Chow ,  Austin Payne ,  Kyle Gleason ,  Brian Boniface ,  John Connolly ,  Sam Tuck

    IPC: B01L3/00 ,  C12M1/00 ,  G01N33/574 ,  C12Q1/6816 ,  G01N33/543 ,  G01N33/569 ,  G01N15/14

    Abstract: A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir superior to the array of wells through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: capturing a population of non-cell particles into the array of wells in single-particle format; releasing, from the non-cell particles, a set of probes into the array of wells; capturing a population of cells into the array of wells in single-cell format; releasing biomolecules from each captured cell into the array of wells; and generating a set of genetic complexes comprising the biomolecules associated with a single captured cell and a subset of probes within individual wells of the array of wells.

    System for imaging captured cells
    3.
    发明授权
    System for imaging captured cells 有权

    公开(公告)号：US12222345B2

    公开(公告)日：2025-02-11

    申请号：US15931928

    申请日：2020-05-14

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Kalyan Handique ,  Kyle Gleason ,  Christopher Siemer ,  Priyadarshini Gogoi ,  Saedeh Sepehri Javdani ,  Gene Parunak ,  Aaron Kehrer ,  Jon Meines

    IPC: G01N33/483 ,  G01N21/25 ,  G01N21/64 ,  G01N21/76 ,  G01N33/487 ,  G02B21/06 ,  G02B21/26 ,  A61B5/00

    Abstract: A system for imaging captured cells comprising: an illumination module configured to illuminate a target object; a platform configured to position the target object in relation to the illumination module; a filter module configured to filter light transmitted to the target object and/or to filter light received from the target object, an optical sensor configured to receive light from the target object and to generate image data; and a focusing and optics module configured to manipulate light transmitted to the optical sensor. The system can further comprise one or more of: a control system configured to control at least one of the illumination module, the platform, the focusing and optics module, the filter module, and the optical sensor; a tag identifying system configured to identify and communicate tag information from system elements; a thermal control module configured to adjust temperature parameters of the system; and an image stabilization module.

    SYSTEM AND METHOD FOR ISOLATING AND ANALYZING CELLS
    5.
    发明申请
    SYSTEM AND METHOD FOR ISOLATING AND ANALYZING CELLS 有权

    公开(公告)号：US20230037836A1

    公开(公告)日：2023-02-09

    申请号：US17965495

    申请日：2022-10-13

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Kalyan Handique ,  Vishal Sharma ,  Priyadarshini Gogoi ,  William Chow ,  Austin Payne ,  Kyle Gleason ,  Brian Boniface ,  John Connolly ,  Sam Tuck

    IPC: B01L3/00 ,  C12M1/00 ,  G01N33/574 ,  C12Q1/6816 ,  G01N33/543 ,  G01N33/569 ,  G01N15/14

    Abstract: A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir superior to the array of wells through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: capturing a population of non-cell particles into the array of wells in single-particle format; releasing, from the non-cell particles, a set of probes into the array of wells; capturing a population of cells into the array of wells in single-cell format; releasing biomolecules from each captured cell into the array of wells; and generating a set of genetic complexes comprising the biomolecules associated with a single captured cell and a subset of probes within individual wells of the array of wells.

    System and method for capturing and analyzing cells
    6.
    发明授权
    System and method for capturing and analyzing cells 有权

    公开(公告)号：US11345951B2

    公开(公告)日：2022-05-31

    申请号：US16048975

    申请日：2018-07-30

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Kalyan Handique ,  Priyadarshini Gogoi ,  Saedeh Sepehri Javdani ,  Yi Zhou

    IPC: C12Q1/68 ,  C12Q1/6841 ,  G01N33/574 ,  C12Q1/6844 ,  C12Q1/686 ,  G01N33/487 ,  G01N27/447

    Abstract: A system and method for capturing and analyzing a set of cells, comprising: an array including a set of parallel pores, each pore including a chamber including a chamber inlet and a chamber outlet, and configured to hold a single cell, and a pore channel fluidly connected to the chamber outlet; an inlet channel fluidly connected to each chamber inlet of the set of parallel pores; an outlet channel fluidly connected to each pore channel of the set of parallel pores; a set of electrophoresis channels fluidly coupled to the outlet channel, configured to receive a sieving matrix for electrophoretic separation; and a set of electrodes including a first electrode and a second electrode, wherein the set of electrodes is configured to provide an electric field that facilitates electrophoretic analysis of the set of cells.

    SYSTEM AND METHOD FOR ISOLATING AND ANALYZING CELLS
    9.
    发明申请
    SYSTEM AND METHOD FOR ISOLATING AND ANALYZING CELLS 有权

    公开(公告)号：US20210040567A1

    公开(公告)日：2021-02-11

    申请号：US17077095

    申请日：2020-10-22

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Kalyan Handique ,  Priyadarshini Gogoi ,  Yi Zhou ,  Saedeh Sepehri ,  Christopher Siemer

    IPC: C12Q1/6886 ,  C12Q1/6841 ,  G01N33/574 ,  B01L3/00 ,  C12Q1/6834

    Abstract: A system and method for isolating cells, comprising: a substrate having a broad surface; an array comprising a set of wells defined at the broad surface of the substrate, each well including: a base surface, an open surface directly opposing the base surface, defined at the broad surface of the substrate, and configured to receive one of a single cell and a single cluster of cells from a direction perpendicular to the broad surface of the substrate, and a set of channels that fluidly couple each well to at least one adjacent well; wherein the set of wells includes an interior subset and an exterior subset fluidly coupled to and surrounding the interior subset by way of the set of channels; and a fluid delivery module surrounding the array and fluidly coupled to each well in the set of wells.

    SYSTEM FOR IMAGING CAPTURED CELLS
    10.
    发明申请
    SYSTEM FOR IMAGING CAPTURED CELLS 审中-公开

    公开(公告)号：US20200271637A1

    公开(公告)日：2020-08-27

    申请号：US15931928

    申请日：2020-05-14

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Kalyan Handique ,  Kyle Gleason ,  Christopher Siemer ,  Priyadarshini Gogoi ,  Saedeh Sepehri Javdani ,  Gene Parunak ,  Aaron Kehrer ,  Jon Meines

    IPC: G01N33/483 ,  G01N21/64

    Abstract: A system for imaging captured cells comprising: an illumination module configured to illuminate a target object; a platform configured to position the target object in relation to the illumination module; a filter module configured to filter light transmitted to the target object and/or to filter light received from the target object, an optical sensor configured to receive light from the target object and to generate image data; and a focusing and optics module configured to manipulate light transmitted to the optical sensor. The system can further comprise one or more of: a control system configured to control at least one of the illumination module, the platform, the focusing and optics module, the filter module, and the optical sensor; a tag identifying system configured to identify and communicate tag information from system elements; a thermal control module configured to adjust temperature parameters of the system; and an image stabilization module.

Patent Agency Ranking