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9 条记录 (耗时 0.023 秒)

    FUNGAL CELL WITH IMPROVED PROTEIN PRODUCTION CAPACITY
    3.
    发明申请
    FUNGAL CELL WITH IMPROVED PROTEIN PRODUCTION CAPACITY 审中-公开

    公开(公告)号：US20200299742A1

    公开(公告)日：2020-09-24

    申请号：US16634267

    申请日：2018-07-30

    申请人： BIOPETROLIA AB

    发明人： Mingtao Huang ,  Anastasia Krivoruchko ,  Florian David ,  Jens Nielsen

    IPC分类号： C12P21/00 ,  C07K14/395

    摘要： The present invention related to the provision of genetically modified fungal cells, such as yeast cells with an improved ability for producing and secreting different recombinant proteins. The improved ability is obtained by disruption in intracellular transport between the Golgi and the endosome. In particular embodiments, the disruption is achieved by downregulation or deletion of the gene encoding a Tda3p homolog. The fungal cell and method of the invention would allow for large-scale production of recombinant proteins in fungal cells.

    L-ornithine production in eukaryotic cells
    6.
    发明授权
    L-ornithine production in eukaryotic cells 有权

    公开(公告)号：US10544435B2

    公开(公告)日：2020-01-28

    申请号：US15556737

    申请日：2016-03-10

    申请人： BIOPETROLIA AB

    发明人： Jiufu Qin ,  Anastasia Krivoruchko ,  Florian David ,  Bo Jiang ,  Jens Nielsen

    IPC分类号： C12P13/10 ,  C12N9/02 ,  C12N9/10 ,  C12N9/12 ,  C12N9/78 ,  C12N9/88 ,  C12N13/00 ,  C12N15/52 ,  C12P13/00

    摘要： The present invention relates to the provision of genetically modified microbial cells, such as yeast cells with an improved ability for producing L-ornithine and its derivatives. Overproduction of L-ornithine is obtained in the first place by the down-regulation or attenuation of specially selected genes, wherein said genes encode enzymes involved in the L-ornithine consumption and/or degradation pathways. Further L-ornithine production ability is improved by down-regulation, attenuation, deletion or overexpression of specially selected genes, wherein said genes encode enzymes and/or proteins involved in the L-ornithine ‘acetylated derivatives cycle’, L-glutamate synthesis pathways, subcellular trafficking, TCA cycle, pyruvate carboxylation pathway, respiratory electron-transport chain, and the carbon substrates' assimilation machinery. The invention additionally provides a method to produce L-ornithine with said modified eukaryotic cells.