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公开(公告)号：US5965409A
公开(公告)日：1999-10-12
申请号：US684547
申请日：1996-07-19
Applicant: Arthur B. Pardee , Peng Liang
Inventor： Arthur B. Pardee , Peng Liang
CPC classification number: C12N15/1096 , C12N15/101 , C12Q1/6809 , C12Q1/686 , Y10S435/81
Abstract: A system for isolating mRNAs as cDNAs employs a polymerase amplification method using at least two oligodeoxynucleotide primers. In one approach, the first primer contains sequence capable of hybridizing to a site immediately upstream of the first A ribonucleotide of the mRNA's polyA tail and the second primer contains arbitrary sequence. In another approach, the first primer contains sequence capable of hybridizing to a site including the mRNA's polyA signal sequence and the second primer contains arbitrary sequence. In another approach, the first primer contains arbitrary sequence and the second primer contains sequence capable of hybridizing to a site including the Kozak sequence. In another approach, the first primer contains a sequence that is substantially complementary to the sequence of a mRNA having a known sequence and the second primer contains arbitrary sequence. In another approach, the first primer contains arbitrary sequence and the second primer contains sequence that is substantially identical to the sequence of a mRNA having a known sequence. The first primer is used as a primer for reverse transcription of the MRNA and the resultant cDNA is amplified with a polymerase using both the first and second primers as a primer set.
Abstract translation: 用于将mRNA分离为cDNA的系统采用使用至少两个寡脱氧核苷酸引物的聚合酶扩增方法。 在一种方法中，第一引物含有能够与mRNA的polyA尾部的第一个A核糖核苷酸紧邻上游位点杂交的序列，第二个引物含有任意序列。 在另一种方法中，第一引物含有能够与包含mRNA的polyA信号序列的位点杂交的序列，第二引物含有任意序列。 在另一种方法中，第一引物含有任意序列，第二引物含有能够与包括Kozak序列的位点杂交的序列。 在另一种方法中，第一引物含有与具有已知序列的mRNA的序列基本互补的序列，第二引物含有任意序列。 在另一种方法中，第一引物含有任意序列，第二引物含有与具有已知序列的mRNA的序列基本相同的序列。 第一引物用作MRNA的逆转录的引物，并且使用第一和第二引物作为引物组，用聚合酶扩增得到的cDNA。
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公开(公告)号：US5262311A
公开(公告)日：1993-11-16
申请号：US850343
申请日：1992-03-11
Applicant: Arthur B. Pardee , Peng Liang
Inventor： Arthur B. Pardee , Peng Liang
CPC classification number: C12N15/1096 , C12N15/101 , C12Q1/686
Abstract: A method for isolating mRNAs as cDNAs employs a polymerase amplification method using at least two oligodeoxynucleotide primers, one being short with arbitrary sequence and another being either short with arbitrary sequence or being capable of hybridizing to the region near the mRNA polyA tail. The oligodeoxynucleotide that is capable of hybridizing to the region near the polyA tail is used as a primer for reverse transcription of the mRNA and the resultant cDNA is amplified with a polymerase using both oligodeoxynucleotides as a primer set.
Abstract translation: 将mRNA分离为cDNA的方法采用使用至少两个寡脱氧核苷酸引物的聚合酶扩增方法，其一个具有任意序列的短序列，另一个短序列具有任意序列或能够与mRNA polyA尾部附近的区域杂交。 能够与polyA尾部附近的区域杂交的寡脱氧核苷酸用作mRNA逆转录的引物，用寡聚脱氧核苷酸作为引物组，用聚合酶扩增得到的cDNA。
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公开(公告)号：US5665547A
公开(公告)日：1997-09-09
申请号：US430536
申请日：1995-04-25
Applicant: Arthur B. Pardee , Peng Liang
Inventor： Arthur B. Pardee , Peng Liang
CPC classification number: C12N15/1096 , C12N15/101 , C12Q1/6809 , C12Q1/686 , Y10S435/81
Abstract: A method for comparing amounts or levels mRNAs employs a polymerase amplification method using at least two oligodeoxynucleotide primers. In one approach, the first primer contains sequence capable of hybridizing to a site immediately upstream of the first A ribonucleotide of the mRNA's polyA tail and the second primer contains arbitrary sequence. In another approach, the first primer contains sequence capable of hybridizing to a site including the mRNA's polyA signal sequence and the second primer contains arbitrary sequence. In another approach, the first primer contains arbitrary sequence and the second primer contains sequence capable of hybridizing to a site including the Kozak sequence. In another approach, the first primer contains a sequence that is substantially complementary to the sequence of a mRNA having a known sequence and the second primer contains arbitrary sequence. In another approach, the first primer contains arbitrary sequence and the second primer contains sequence that is substantially identical to the sequence of a mRNA having a known sequence. The first primer is used as a primer for reverse transcription of the mRNA and the resultant cDNA is amplified with a polymerase using both the first and second primers as a primer set.
Abstract translation: 用于比较数量或水平的mRNA的方法使用使用至少两个寡脱氧核苷酸引物的聚合酶扩增方法。 在一种方法中，第一引物含有能够与mRNA的polyA尾部的第一个A核糖核苷酸紧邻上游位点杂交的序列，第二个引物含有任意序列。 在另一种方法中，第一引物含有能够与包含mRNA的polyA信号序列的位点杂交的序列，第二引物含有任意序列。 在另一种方法中，第一引物含有任意序列，第二引物含有能够与包括Kozak序列的位点杂交的序列。 在另一种方法中，第一引物含有与具有已知序列的mRNA的序列基本互补的序列，第二引物含有任意序列。 在另一种方法中，第一引物含有任意序列，第二引物含有与具有已知序列的mRNA的序列基本相同的序列。 将第一引物用作mRNA逆转录的引物，并用聚合酶扩增得到的cDNA，使用第一和第二引物作为引物组。
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公开(公告)号：US5599672A
公开(公告)日：1997-02-04
申请号：US351748
申请日：1994-12-08
Applicant: Peng Liang , Arthur B. Pardee , Cesario F. Bianchi
Inventor： Peng Liang , Arthur B. Pardee , Cesario F. Bianchi
CPC classification number: C12N15/1096 , C12N15/101 , C12Q1/6809 , C12Q1/686 , Y10S435/81
Abstract: A method for isolating mRNAs as cDNAs employs a polymerase amplification method using at least two oligodeoxynucleotide primers. In one approach, the first primer contains sequence capable of hybridizing to a site immediately upstream of the first A ribonucleotide of the mRNA's polyA tail and the second primer contains arbitrary sequence. In another approach, the first primer contains sequence capable of hybridizing to a site including the mRNA's polyA signal sequence and the second primer contains arbitrary sequence. In another approach, the first primer contains arbitrary sequence and the second primer contains sequence capable of hybridizing to a site including the Kozak sequence. In another approach, the first primer contains a sequence that is substantially complementary to the sequence of a mRNA having a known sequence and the second primer contains arbitrary sequence. In another approach, the first primer contains arbitrary sequence and the second primer contains sequence that is substantially identical to the sequence of a mRNA having a known sequence. The first primer is used as a primer for reverse transcription of the mRNA and the resultant cDNA is amplified with a polymerase using both the first and second primers as a primer set.
Abstract translation: 将mRNA分离为cDNA的方法采用使用至少两个寡脱氧核苷酸引物的聚合酶扩增方法。 在一种方法中，第一引物含有能够与mRNA的polyA尾部的第一个A核糖核苷酸紧邻上游位点杂交的序列，第二个引物含有任意序列。 在另一种方法中，第一引物含有能够与包含mRNA的polyA信号序列的位点杂交的序列，第二引物含有任意序列。 在另一种方法中，第一引物含有任意序列，第二引物含有能够与包括Kozak序列的位点杂交的序列。 在另一种方法中，第一引物含有与具有已知序列的mRNA的序列基本互补的序列，第二引物含有任意序列。 在另一种方法中，第一引物含有任意序列，第二引物含有与具有已知序列的mRNA的序列基本相同的序列。 将第一引物用作mRNA逆转录的引物，并用聚合酶扩增得到的cDNA，使用第一和第二引物作为引物组。
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公开(公告)号：USD975356S1
公开(公告)日：2023-01-10
申请号：US29854118
申请日：2022-09-22
Applicant: Peng Liang
Designer： Peng Liang
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Patent Agency Ranking
公开(公告)号：US07576191B2
公开(公告)日：2009-08-18
申请号：US11519193
申请日：2006-09-11
Applicant: Peng Liang , Yong-jig Cho
Inventor： Peng Liang , Yong-jig Cho
IPC: C07H21/02
CPC classification number: G01N33/57484 , C07K14/4747 , C12Q1/6886 , C12Q2600/136 , C12Q2600/156 , G01N2333/4704
Abstract: The present invention relates to a new tumor suppressor, designated Killin. Also described are diagnostic and therapeutic uses of the Killin protein and the killin gene, alone or in combination with traditional cancer therapies.
Abstract translation: 本发明涉及称为Killin的新的肿瘤抑制因子。 还描述了单独的或与传统癌症治疗组合的Killin蛋白和杀伤细胞基因的诊断和治疗用途。
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公开(公告)号：US20100286846A1
公开(公告)日：2010-11-11
申请号：US12810223
申请日：2008-06-30
Applicant: Fenghua Liang , Peng Liang , Jugang He
Inventor： Fenghua Liang , Peng Liang , Jugang He
Abstract: A vehicle central lock antitheft method and system includes a central lock system which is connected by radio with a remote controller for identity verification using rolling codes (S101, S102), wherein the central lock system chooses a security state (S103) and transmits the security state to an engine management system according to the verification result, the ignition IG status and the door switch status signal indicating opening or closing of the door (S104). The engine management system receives the security state transmitted by the central lock system and verifies the identity of the central lock system by code matching (S106); security identification between the engine management system and the central lock system is carried out by bidirectional encryption communication (S107). The engine management system then decides whether or not to lock the engine according to the result of the security identification.
Abstract translation: 一种车辆中央锁防盗方法和系统，包括通过无线电与遥控器连接的中央锁防盗系统，用于使用滚动码进行身份验证（S101，S102），其中中央锁系统选择安全状态（S103），并发送安全 根据验证结果指示发动机管理系统，点火IG状态和指示门的打开或关闭的门开关状态信号（S104）。 引擎管理系统接收中央锁系统发送的安全状态，并通过码匹配验证中央锁系统的身份（S106）; 引擎管理系统与中央锁系统之间的安全识别通过双向加密通信进行（S107）。 然后，发动机管理系统根据安全标识的结果来决定是否锁定发动机。
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公开(公告)号：US20080311131A1
公开(公告)日：2008-12-18
申请号：US11892613
申请日：2007-08-24
Applicant: Peng Liang
Inventor： Peng Liang
IPC: A61K39/395 , A61P35/00
CPC classification number: G01N33/57419 , C07K14/54 , C07K14/7155 , C07K16/244 , G01N33/5011 , G01N33/574 , G01N33/57446 , G01N2333/715
Abstract: The present invention provides the receptors for Mob-5 (IL-24). One of the Mob-5 receptors comprises IL-22R1 and IL-20R2. Another Mob-5 receptor comprises IL-20R1 and IL-22R2. The invention also provides methods of inhibiting the Mob-5 receptor as well as methods of detecting cancer by detecting the presence of the Mob-5 receptor.
Abstract translation: 本发明提供了用于Mob-5（IL-24）的受体。 Mob-5受体之一包含IL-22R1和IL-20R2。 另一种Mob-5受体包含IL-20R1和IL-22R2。 本发明还提供了通过检测Mob-5受体的存在来抑制Mob-5受体的方法以及检测癌症的方法。
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公开(公告)号：US07276237B2
公开(公告)日：2007-10-02
申请号：US11124925
申请日：2005-05-09
Applicant: Peng Liang
Inventor： Peng Liang
IPC: A61K39/395 , C07K16/30
CPC classification number: G01N33/57419 , C07K14/54 , C07K14/7155 , C07K16/244 , G01N33/5011 , G01N33/574 , G01N33/57446 , G01N2333/715
Abstract: The present invention provides the receptors for Mob-5 (IL-24). One of the Mob-5 receptors comprises IL-22R1 and IL-20R2. Another Mob-5 receptor comprises IL-20R1 and IL-22R2. The invention also provides methods of inhibiting the Mob-5 receptor as well as methods of detecting cancer by detecting the presence of the Mob-5 receptor.
Abstract translation: 本发明提供了用于Mob-5（IL-24）的受体。 Mob-5受体之一包含IL-22R1和IL-20R2。 另一种Mob-5受体包含IL-20R1和IL-22R2。 本发明还提供了通过检测Mob-5受体的存在来抑制Mob-5受体的方法以及检测癌症的方法。
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10.发明申请Methods and compositions for producing secreted trimeric receptor analogs and biologically active fusion proteins 失效用于产生分泌的三聚体受体类似物和生物活性融合蛋白的方法和组合物公开(公告)号：US20070087413A1
公开(公告)日：2007-04-19
申请号：US11643568
申请日：2006-12-21
Applicant: Peng Liang
Inventor： Peng Liang
CPC classification number: C07K14/7151 , C07K14/70578 , C07K14/78 , C07K2319/32 , C07K2319/735
Abstract: Methods and compositions for producing secreted soluble receptors and biologically active polypeptides in trimeric forms are disclosed. The process involves fusing the DNA template encoding a soluble receptor with a ligand binding domain or biologically active polypeptide to a DNA sequence encoding a C-propeptide of collagen, which is capable of self-assembly into a covalently linked trimer. The resulting fusion proteins are secreted as trimeric soluble receptor analogs, which can be used for more efficient neutralization of the biological activities of their naturally occurring trimeric ligands.
Abstract translation: 公开了用于产生三聚体形式的分泌的可溶性受体和生物活性多肽的方法和组合物。 该方法包括将编码可溶性受体的DNA模板与配体结合结构域或生物活性多肽融合到能够自组装成共价连接的三聚体的编码胶原蛋白的C-前肽的DNA序列。 所得融合蛋白作为三聚体可溶性受体类似物分泌，可用于更有效地中和其天然存在的三聚体配体的生物活性。
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