公开(公告)号：US5204096A

公开(公告)日：1993-04-20

申请号：US338028

申请日：1989-04-14

申请人： Alexander R. Neurath ,  Stephen B. H. Kent

发明人： Alexander R. Neurath ,  Stephen B. H. Kent

IPC分类号： A61K9/127 ,  A61K39/00 ,  A61K47/48 ,  C07K14/02 ,  C07K16/08 ,  G01N33/576

CPC分类号： C07K14/005 ,  A61K47/48176 ,  A61K47/48315 ,  A61K47/48815 ,  A61K47/48876 ,  A61K9/1271 ,  C07K16/082 ,  G01N33/5764 ,  A61K39/00 ,  C12N2730/10122 ,  Y10S530/81

摘要： A hepatitis B vaccine containing a peptide with an amino acid chain of at least six consecutive amino acids within the pre-S gene coded region of the envelope of hepatitis B virus. The vaccine being free of an amino acid sequence corresponding to the naturally occurring envelope proteins of hepatitis B virus and a physiologically acceptable diluent. The peptide being free or linked to a carrier. The carrier being a conventional carrier or a novel carrier including a lipid vesicle stabilized by cross-linking and having covalently bonded active sites on the outer surface thereon. Such novel carrier being useful not only to link the novel peptide containing an amino acid chain with amino acids within the pre-S gene coded region of the surface antigen or hepatitis B virus, but can also be used to bind synthetic peptide analogues of other viral proteins, as well as bacterial, allergen and parasitic proteins of man and animals. The peptides of the invention can be utilized in diagnostics for the detection of antigens and antibodies.

摘要翻译： 一种乙型肝炎疫苗，其含有乙型肝炎病毒包膜前S基因编码区内具有至少六个连续氨基酸的氨基酸链的肽。 该疫苗不含对应于乙型肝炎病毒的天然存在的包膜蛋白的氨基酸序列和生理上可接受的稀释剂。 肽与载体无关或连接。 载体是常规载体或包含通过交联稳定并在其外表面上具有共价键合的活性位点的脂质囊泡的新型载体。 这种新型载体不仅用于将含有氨基酸链的新肽与表面抗原或乙型肝炎病毒的S前编码区之前的氨基酸连接，还可用于结合其他病毒的合成肽类似物 蛋白质，以及人和动物的细菌，变应原和寄生蛋白。 本发明的肽可用于诊断抗原和抗体的检测。

公开(公告)号：US4861588A

公开(公告)日：1989-08-29

申请号：US856522

申请日：1986-04-28

申请人： Alexander R. Neurath ,  Stephen B. H. Kent

发明人： Alexander R. Neurath ,  Stephen B. H. Kent

IPC分类号： C12P21/00 ,  A61K9/127 ,  A61K39/00 ,  A61K39/29 ,  A61K47/48 ,  C07K7/06 ,  C07K7/08 ,  C07K14/00 ,  C07K14/02 ,  C07K14/10 ,  C07K14/18 ,  C07K14/705 ,  C07K16/08 ,  C12Q1/16 ,  G01N33/53 ,  G01N33/576

CPC分类号： C07K14/005 ,  A61K47/48176 ,  A61K47/48315 ,  A61K47/48815 ,  A61K9/1271 ,  C07K16/082 ,  G01N33/5761 ,  A61K39/00 ,  C12N2730/10122 ,  Y10S930/223

摘要： A hepatitis B vaccine containing a peptide with an amino acid chain of at least six consecutive amino acids within the pre-S gene coded region of the envelope of hepatitis B virus. The vaccine being free of an amino acid sequence corresponding to the naturally occurring envelope proteins of hepatitis B virus and a physiologically acceptable diluent. The peptide being free or linked to a carrier. The carrier being a conventional carrier or a novel carrier including a lipid vesicle stabilized by cross-linking and having covalently bonded active sites on the outer surface thereon. Such novel carrier being useful not only to link the novel peptide containing an amino acid chain with amino acids within the pre-S gene coded region of the surface antigen of hepatitis B virus, but can also be used to bind synthetic peptide analogues of other viral proteins, as well as bacterial, allergen and parasitic proteins of man and animals. The peptides of the invention can be utilized in diagnostics for the detection of antigens and antibodies.

摘要翻译： 一种乙型肝炎疫苗，其含有乙型肝炎病毒包膜前S基因编码区内具有至少六个连续氨基酸的氨基酸链的肽。 该疫苗不含对应于乙型肝炎病毒的天然存在的包膜蛋白的氨基酸序列和生理上可接受的稀释剂。 肽与载体无关或连接。 载体是常规载体或包含通过交联稳定并在其外表面上具有共价键合的活性位点的脂质囊泡的新型载体。 这种新型载体不仅可用于将含有氨基酸链的新肽与乙型肝炎病毒表面抗原前S基因编码区内的氨基酸连接，还可用于结合其他病毒的合成肽类似物 蛋白质，以及人和动物的细菌，变应原和寄生蛋白。 本发明的肽可用于诊断抗原和抗体的检测。

公开(公告)号：US4847080A

公开(公告)日：1989-07-11

申请号：US698499

申请日：1985-02-05

申请人： Alexander R. Neurath ,  Stephen B. H. Kent

发明人： Alexander R. Neurath ,  Stephen B. H. Kent

IPC分类号： G01N33/576 ,  A61K9/127 ,  A61K39/00 ,  A61K39/29 ,  A61K39/385 ,  A61K47/48 ,  C07K7/04 ,  C07K14/00 ,  C07K14/02 ,  C07K14/10 ,  C07K14/18 ,  C07K14/435 ,  C07K16/08 ,  C07K19/00 ,  C12N15/00 ,  C12N15/09 ,  C12P21/00 ,  C12P21/08 ,  G01N33/543 ,  G01N33/577

CPC分类号： C07K14/005 ,  A61K47/48176 ,  A61K47/48315 ,  A61K47/48815 ,  A61K9/1271 ,  C07K16/082 ,  G01N33/5764 ,  A61K39/00 ,  C12N2730/10122 ,  Y10S930/221 ,  Y10S930/222 ,  Y10S930/223 ,  Y10S930/26 ,  Y10S930/27

摘要： A hepatitis B vaccine containing a peptide with an amino acid chain of at least six consecutive amino acids within the pre-S gene coded region of the envelope of hepatitis B virus. The vaccine being free of an amino acid sequence corresponding to the naturally occurring envelope proteins of hepatitis B virus and a physiologically acceptable diluent. The peptide being free or linked to a carrier. The carrier being a conventional carrier or a novel carrier including a lipid vesicle stabilized by cross-linking and having covalently bonded active sites on the outer surface thereon. Such novel carrier being useful not only to link the novel peptide containing an amino acid chain with amino acids within the pre-S gene coded region of the surface antigen of hepatitis B virus, but can also be used to bind synthetic peptide analogues of other viral proteins, as well as bacterial, allergen and parasitic proteins of man and animals. The peptides of the invention can be utilized in diagnostics for the detection of antigens and antibodies.V

公开(公告)号：US5565548A

公开(公告)日：1996-10-15

申请号：US31735

申请日：1993-03-15

申请人： Alexander R. Neurath ,  Stephen B. H. Kent

发明人： Alexander R. Neurath ,  Stephen B. H. Kent

IPC分类号： A61K9/127 ,  A61K39/00 ,  A61K47/48 ,  C07K14/02 ,  C07K16/08 ,  G01N33/576 ,  A61K38/00 ,  A61K45/00 ,  C17K5/00

CPC分类号： C07K14/005 ,  A61K47/48176 ,  A61K47/48315 ,  A61K47/48815 ,  A61K47/48876 ,  A61K9/1271 ,  C07K16/082 ,  G01N33/5764 ,  A61K39/00 ,  C12N2730/10122 ,  Y10S530/81

摘要： A hepatitis B vaccine containing a peptide with an amino acid chain of at least six consecutive amino acids within the pre-S gene coded region of the envelope of hepatitis B virus. The vaccine being free of an amino acid sequence corresponding to the naturally occurring envelope proteins of hepatitis B virus and a physiologically acceptable diluent. The peptide being free or linked to a carrier. The carrier being a conventional carrier or a novel carrier including a lipid vesicle stabilized by cross-linking and having covalently bonded active sites on the outer surface thereon. Such novel carrier being useful not only to link the novel peptide containing an amino acid chain with amino acids within the pre-S gene coded region of the surface antigen of hepatitis B virus, but can also be used to bind synthetic peptide analogues of other viral proteins, as well as bacterial, allergen and parasitic proteins of man and animals. The peptides of the invention can be utilized in diagnostics for the detection of antigens and antibodies.

摘要翻译： 一种乙型肝炎疫苗，其含有乙型肝炎病毒包膜前S基因编码区内具有至少六个连续氨基酸的氨基酸链的肽。 该疫苗不含对应于乙型肝炎病毒的天然存在的包膜蛋白的氨基酸序列和生理上可接受的稀释剂。 肽与载体无关或连接。 载体是常规载体或包含通过交联稳定并在其外表面上具有共价键合的活性位点的脂质囊泡的新型载体。 这种新型载体不仅可用于将含有氨基酸链的新肽与乙型肝炎病毒表面抗原前S基因编码区内的氨基酸连接，还可用于结合其他病毒的合成肽类似物 蛋白质，以及人和动物的细菌，变应原和寄生蛋白。 本发明的肽可用于诊断抗原和抗体的检测。

公开(公告)号：US5158769A

公开(公告)日：1992-10-27

申请号：US337784

申请日：1989-04-13

申请人： Alexander R. Neurath ,  Stephen B. H. Kent

发明人： Alexander R. Neurath ,  Stephen B. H. Kent

IPC分类号： A61K9/127 ,  A61K39/00 ,  A61K47/48 ,  C07K14/02 ,  C07K16/08 ,  G01N33/576

CPC分类号： C07K14/005 ,  A61K47/48176 ,  A61K47/48315 ,  A61K47/48815 ,  A61K9/1271 ,  B82Y5/00 ,  C07K16/082 ,  G01N33/5761 ,  G01N33/5764 ,  A61K39/00 ,  C12N2730/10122

摘要： A hepatitis B vaccine containing a peptide with an amino acid chain of at least six consecutive amino acids within the pre-S gene coded region of the envelope of hepatitis B virus. The vaccine being free of an amino acid sequence corresponding to the naturally occurring envelope proteins of hepatitis B virus and a physiologically acceptable diluent. The peptide being free or linked to a carrier. The carrier being a conventional carrier or a novel carrier including a lipid vesicle stabilized by cross-linking and having covalently bonded active sites on the outer surface thereon. Such novel carrier being useful not only to link the novel peptide containing an amino acid chain with amino acids within the pre-S gene coded region of the surface antigen of hepatitis B virus, but can also be used to bind synthetic peptide analogues of other viral proteins, as well as bacterial, allergen and parasitic proteins of man and animals. The peptides of the invention can be utilized in diagnostics for the detection of antigens and antibodies.

摘要翻译： 一种乙型肝炎疫苗，其含有乙型肝炎病毒包膜前S基因编码区内具有至少六个连续氨基酸的氨基酸链的肽。 该疫苗不含对应于乙型肝炎病毒的天然存在的包膜蛋白的氨基酸序列和生理上可接受的稀释剂。 肽与载体无关或连接。 载体是常规载体或包含通过交联稳定并在其外表面上具有共价键合的活性位点的脂质囊泡的新型载体。 这种新型载体不仅可用于将含有氨基酸链的新肽与乙型肝炎病毒表面抗原前S基因编码区内的氨基酸连接，还可用于结合其他病毒的合成肽类似物 蛋白质，以及人和动物的细菌，变应原和寄生蛋白。 本发明的肽可用于诊断抗原和抗体的检测。

公开(公告)号：US4803156A

公开(公告)日：1989-02-07

申请号：US774829

申请日：1985-09-11

申请人： Alexander R. Neurath ,  Stephen B. H. Kent ,  Nathan Strick

发明人： Alexander R. Neurath ,  Stephen B. H. Kent ,  Nathan Strick

IPC分类号： C07K14/02 ,  C07K14/16 ,  G01N33/535 ,  G01N33/576 ,  G01N33/53

CPC分类号： C07K14/005 ,  G01N33/535 ,  G01N33/5761 ,  C12N2730/10122 ,  C12N2740/16222 ,  Y10S436/82 ,  Y10S436/828 ,  Y10S930/142 ,  Y10S930/221 ,  Y10S930/222 ,  Y10S930/223 ,  Y10S930/26 ,  Y10S930/31

摘要： A reagent for an ELISA determination of an antibody, the reagent comprising a peptide covalently linked to beta-lactamase. The reagent can be used in the following method to detect antibodies in a sample which involvesa. contacting the sample with protein A linked to a solid support,b. incubating the sample-protein A linked to the solid support,c. washing the incubated sample-protein A linked to the solid support,d. contacting the washed sample-protein A with the reagent,e. incubating the sample-protein A and reagent,f. washing the incubated sample-protein A-reagent, andg. determining the enzymatic activity of the resultant mass.

摘要翻译： 用于ELISA测定抗体的试剂，所述试剂包含与β-内酰胺酶共价连接的肽。 试剂可以按照以下方法用于检测涉及a的样品中的抗体。 使样品与连接到固体支持物的蛋白A接触，b。 孵育与固体支持物连接的样品蛋白A，c。 洗涤与固体支持物连接的温育样品 - 蛋白A，d。 将洗涤过的样品蛋白A与试剂接触， 孵育样品蛋白A和试剂，f。 洗涤孵育的样品蛋白A试剂，g。 测定所得物质的酶活性。

公开(公告)号：US5620844A

公开(公告)日：1997-04-15

申请号：US57200

申请日：1993-05-03

申请人： Alexander R. Neurath ,  Stephen B. H. Kent

发明人： Alexander R. Neurath ,  Stephen B. H. Kent

IPC分类号： A61K9/127 ,  A61K39/00 ,  A61K47/48 ,  C07K14/02 ,  C07K16/08 ,  G01N33/576 ,  C12Q1/70 ,  A61K38/08 ,  G01N33/53 ,  G01N33/537

CPC分类号： C07K14/005 ,  A61K47/48176 ,  A61K47/48315 ,  A61K47/48815 ,  A61K9/1271 ,  B82Y5/00 ,  C07K16/082 ,  G01N33/5761 ,  G01N33/5764 ,  A61K39/00 ,  C12N2730/10122

摘要： A hepatitis B vaccine containing a peptide with an amino acid chain of at least six consecutive amino acids within the pre-S gene coded region of the envelope of hepatitis B virus. The vaccine being free of an amino acid sequence corresponding to the naturally occurring envelope proteins of hepatitis B virus and a physiologically acceptable diluent. The peptide being free or linked to a carrier. The carrier being a conventional carrier or a novel carrier including a lipid vesicle stabilized by cross-linking and having covalently bonded active sites on the outer surface thereon. Such novel carrier being useful not only to link the novel peptide containing an amino acid chain with amino acids within the pre-S gene coded region of the surface antigen of hepatitis B virus, but can also be used to bind synthetic peptide analogues of other viral proteins, as well as bacterial, allergen and parasitic proteins of man and animals. The peptides of the invention can be utilized in diagnostics for the detection of antigens and antibodies.

摘要翻译： 一种乙型肝炎疫苗，其含有乙型肝炎病毒包膜前S基因编码区内具有至少六个连续氨基酸的氨基酸链的肽。 该疫苗不含对应于乙型肝炎病毒的天然存在的包膜蛋白的氨基酸序列和生理上可接受的稀释剂。 肽与载体无关或连接。 载体是常规载体或包含通过交联稳定并在其外表面上具有共价键合的活性位点的脂质囊泡的新型载体。 这种新型载体不仅可用于将含有氨基酸链的新肽与乙型肝炎病毒表面抗原前S基因编码区内的氨基酸连接，还可用于结合其他病毒的合成肽类似物 蛋白质，以及人和动物的细菌，变应原和寄生蛋白。 本发明的肽可用于诊断抗原和抗体的检测。

公开(公告)号：US3962421A

公开(公告)日：1976-06-08

申请号：US507306

申请日：1974-09-19

申请人： Alexander R. Neurath

发明人： Alexander R. Neurath

IPC分类号： A61K39/145 ,  A61K39/205 ,  C12N7/06 ,  A61K39/18

CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K39/145 ,  A61K39/205 ,  A61K39/00 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16163 ,  C12N2760/16251 ,  C12N2760/20151

摘要： Virus-containing media are treated and the viruses broken down into sub-units by mixing with trialkyl phosphates in the presence of a wetting agent. In comparison with conventional means, sub-unit vaccines prepared therefrom have low toxic pyrogen content, and influenza viruses of Types A and B are disrupted without substantial decreases in neuraminidase level. The method can be used on viruses contained in relatively dilute allantoic fluids as well as those in concentrated centrifugates.

摘要翻译： 处理含病毒的培养基，通过在润湿剂存在下与磷酸三烷基酯混合，将病毒分解为亚单位。 与常规方法相比，由其制备的亚单位疫苗具有低毒性热原含量，A型和B型流感病毒破坏而神经氨酸酶水平没有显着降低。 该方法可用于相对稀释的尿囊内液体以及浓缩离心液中的病毒。

公开(公告)号：US06727240B1

公开(公告)日：2004-04-27

申请号：US08342745

申请日：1994-10-21

申请人： Alexander R. Neurath ,  Shibo Jiang ,  Asim Kumar Debnath

发明人： Alexander R. Neurath ,  Shibo Jiang ,  Asim Kumar Debnath

IPC分类号： A61K31555

CPC分类号： A61K31/695 ,  A61K31/409 ,  A61K31/765 ,  A61K2300/00

摘要： A method of preventing HIV-1 or HIV-2 infection by administering to a human a pharmaceutically effective anti-HIV-1 or anti-HIV-2 amount of a tin or silicon protoporphyrin IX or tin or silicon mesoporphyrin IX, or a pharmaceutically acceptable salt thereof.

摘要翻译： 一种通过向人施用药学有效的抗HIV-1或抗HIV-2量的锡或硅原卟啉IX或锡或硅中卟啉IX或其药学上可接受的方法来预防HIV-1或HIV-2感染的方法 的盐。

公开(公告)号：US4877725A

公开(公告)日：1989-10-31

申请号：US718140

申请日：1985-04-01

申请人： Alexander R. Neurath ,  Nathan Strick

发明人： Alexander R. Neurath ,  Nathan Strick

IPC分类号： G01N33/569

CPC分类号： G01N33/56988 ,  Y10S435/81 ,  Y10S435/948 ,  Y10S435/974 ,  Y10S530/806 ,  Y10S530/811 ,  Y10S530/812 ,  Y10S530/826

摘要： A process for the detection of antibodies to HTLV III/LAV comprising:(A) mixing an unknown serum sample with a crude HTLV III/LAV viral antigen selected from the group consisting of(1) an antigen comprising P24 core protein and Penv protein;(2) a P24 antigen and(3) a Penv antigen,(B) incubating the resultant mixture from step (A);(C) contacting the mixture of step (B) with a solid substrate coated with antibody to HTLV III/LAV;(D) incubating the mass from step (C);(E) washing the mass from step (D);(F) contacting the mass from step (E) with a labeled antibody to HTLV III/LAV;(G) incubating the mass from step (F);(H) washing the mass from step (G);(I) assaying the label in the mass from step (H);(J) as a negative control, mixing a serum sample known to be negative to HTLV III/LAV antibody with a diluent;(K) subjecting the mass from step (J) to steps (B) to (I);(L) as a positive control, mixing a predetermined amount of the crude HTLV III/LAV viral antigen and the diluent;(M) subjecting the mass from step (L) to steps (B) to (I); and(N) comparing the results of the assay for the label from steps (I), (K) and (M).

摘要翻译： 用于检测HTLV III / LAV的抗体的方法，包括：（A）将未知血清样品与选自（1）包含P24核心蛋白和Penv蛋白的抗原的粗HTLV III / LAV病毒抗原混合; （2）P24抗原和（3）Penv抗原，（B）温育来自步骤（A）的所得混合物; （C）使步骤（B）的混合物与涂覆有HTLV III / LAV抗体的固体基质接触; （D）将来自步骤（C）的质量孵育; （E）从步骤（D）洗涤质量; （F）使来自步骤（E）的质量与HTLV III / LAV的标记抗体接触; （G）温育步骤（F）的质量; （H）从步骤（G）洗涤质量; （I）测定步骤（H）中质量的标记物; （J）作为阴性对照，将已知为阴性的血清样品与稀释剂混合至HTLV III / LAV抗体; （K）使步骤（J）的步骤（B）至（I） （L）作为阳性对照，混合预定量的粗制HTLV III / LAV病毒抗原和稀释剂; （M）使来自步骤（L）的物质经历步骤（B）至（I）; 和（N）比较来自步骤（I），（K）和（M）的标记物的测定结果。