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1.发明授权Bacillus thuringiensis CryET29 compositions toxic to coleopteran insects and Ctenocephalides spp 失效苏云金芽孢杆菌CryET29组合物对鞘翅目昆虫和栉头菜属毒素有毒公开(公告)号：US07572587B2
公开(公告)日：2009-08-11
申请号：US11712704
申请日：2007-03-01
Applicant: Mark J. Rupar , William P. Donovan , Yuping Tan , Annette C. Slaney
Inventor： Mark J. Rupar , William P. Donovan , Yuping Tan , Annette C. Slaney
CPC classification number: A01N63/00 , C07K14/325 , C12N15/8286 , G01N2430/10 , Y02A40/162 , Y10S530/825
Abstract: Disclosed is a novel δ-endotoxin, designated CryET29, that exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea (Ctenocephalides felis), as well as against coleopteran insects, including the southern corn rootworm (Diabrotica undecimpunctata), western corn rootworm (D. virgifera), Colorado potato beetle (Leptinotarsa decemlineata), Japanese beetle (Popillia japonica), and red flour beetle (Tribolium castaneum). Also disclosed are nucleic acid segments encoding CryET29, recombinant vectors, host cells, and transgenic plants comprising a cryET29 DNA segment. Methods for making and using the disclosed protein and nucleic acid segments are disclosed as well as assays and diagnostic kits for detecting cryET29 and CryET29 sequences in vivo and in vitro.
Abstract translation: 公开了一种名为CryET29的新型三角内含物，其表现出对iphonapteran昆虫具有杀虫活性，包括猫跳蚤幼虫（Ctenocephalides felis）以及鞘翅目昆虫，包括南方玉米根虫（Diabrotica undecimpunctata），西方玉米根虫 （D. virgifera），科罗拉多马铃薯甲虫（Leptinotarsa decemlineata），日本甲虫（Popillia japonica）和红粉甲虫（Tribolium castaneum））。 还公开了编码CryET29，重组载体，宿主细胞和包含cryET29 DNA片段的转基因植物的核酸片段。 公开了制备和使用所公开的蛋白质和核酸片段的方法以及用于在体内和体外检测cryET29和CryET29序列的测定和诊断试剂盒。
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2.发明申请Bacillus thuringiensis CryET29 compositions toxic to coleopteran insects and Ctenocephalides SPP. 失效苏云金芽孢杆菌CryET29组合物对鞘翅目昆虫和Ctenocephalides SPP有毒。公开(公告)号：US20070163000A1
公开(公告)日：2007-07-12
申请号：US11712704
申请日：2007-03-01
Applicant: Mark Rupar , William Donovan , Yuping Tan , Annette Slaney
Inventor： Mark Rupar , William Donovan , Yuping Tan , Annette Slaney
CPC classification number: A01N63/00 , C07K14/325 , C12N15/8286 , G01N2430/10 , Y02A40/162 , Y10S530/825
Abstract: Disclosed is a novel δ-endotoxin, designated CryET29, that exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea (Ctenocephalides felis), as well as against coleopteran insects, including the southern corn rootworm (Diabrotica undecimpunctata), western corn rootworm (D. virgifera), Colorado potato beetle (Leptinotarsa decemlineata), Japanese beetle (Popillia japonica), and red flour beetle (Tribolium castaneum). Also disclosed are nucleic acid segments encoding CryET29, recombinant vectors, host cells, and transgenic plants comprising a cryET29 DNA segment. Methods for making and using the disclosed protein and nucleic acid segments are disclosed as well as assays and diagnostic kits for detecting cryET29 and CryET29 sequences in vivo and in vitro.
Abstract translation: 公开了一种名为CryET29的新型三角内含物，其表现出对iphonapteran昆虫具有杀虫活性，包括猫跳蚤幼虫（Ctenocephalides felis）以及鞘翅目昆虫，包括南方玉米根虫（Diabrotica undecimpunctata），西方玉米根虫 （D. virgifera），科罗拉多马铃薯甲虫（Leptinotarsa decemlineata），日本甲虫（Popillia japonica）和红粉甲虫（Tribolium castaneum））。 还公开了编码CryET29，重组载体，宿主细胞和包含cryET29 DNA片段的转基因植物的核酸片段。 公开了制备和使用所公开的蛋白质和核酸片段的方法以及用于在体内和体外检测cryET29和CryET29序列的测定和诊断试剂盒。
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公开(公告)号：US06618679B2
公开(公告)日：2003-09-09
申请号：US09771372
申请日：2001-01-27
Applicant: Christine Loehrlein , Dan Pollart , Thomas Shaler , Kathy Stephens , Yuping Tan , Linda Wong , Joseph Monforte
Inventor： Christine Loehrlein , Dan Pollart , Thomas Shaler , Kathy Stephens , Yuping Tan , Linda Wong , Joseph Monforte
IPC: G06F1900
CPC classification number: C12Q1/6809 , C12Q1/6853 , C12Q1/686 , G06F19/20 , C12Q2537/143 , C12Q2525/15 , C12Q2525/161 , C12Q2525/155
Abstract: This invention provides methods, compositions and kits for gene expression analysis and gene expression profiling. The methods of the invention are highly sensitive; have a wide dynamic range; are rapid and inexpensive; have a high throughput; and allow the simultaneous differential analysis of a defined set of genes. The methods, compositions and kits of the invention also provide tools for gene expression data collection and relational data analysis.
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4.发明授权Bacillus thuringiensis CryET29 compositions toxic to coleopteran insects and ctenocephalides SPP 失效苏云金芽孢杆菌CryET29组合物对鞘翅目昆虫和c螨素SPP有毒
公开(公告)号：US6093695A
公开(公告)日：2000-07-25
申请号：US721259
申请日：1996-09-26
Applicant: Mark J. Rupar , William P. Donovan , Yuping Tan , Annette C. Slaney
Inventor： Mark J. Rupar , William P. Donovan , Yuping Tan , Annette C. Slaney
IPC: C12N15/09 , A01N63/02 , C07K14/325 , C12N1/21 , C12N5/10 , C12N15/32 , C12Q1/68 , C12R1/19 , C12N1/20
CPC classification number: A01N63/00 , C07K14/325 , C12N15/8286 , G01N2430/10 , Y10S530/825
Abstract: Disclosed is a novel .delta.-endotoxin, designated CryET29, that exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea (Ctenocephalides felis), as well as against colcopteran insects, including the southern corn rootworm (Diabrotica undecimpunctata), western corn rootworm (D. virgifera), Colorado potato beetle (Leptinotarsa decemlineata), Japanese beetle (Popillia japonica), and red flour beetle (Tribolium castaneur). Also disclosed are nucleic acid segments encoding CryET29, recombinant vectors, host cells, and transgenic plants comprising a cryET29 DNA segment. Methods for making and using the disclosed protein and nucleic acid segments are disclosed as well as assays and diagnostic kits for detecting cryET29 and CryET29 sequences in vivo and in vitro.
Abstract translation: 公开了一种名为CryET29的新颖的内含肽，其表现出对iphonapteran昆虫具有杀虫活性，包括猫蚤（Ctenocephalides felis）的幼虫，以及对鳞翅目昆虫，包括南方玉米根虫（Diabrotica undecimpunctata），西方玉米根虫 （D. virgifera），科罗拉多马铃薯甲虫（Leptinotarsa decemlineata），日本甲虫（Popillia japonica）和红粉甲虫（Tribolium castaneur）。 还公开了编码CryET29，重组载体，宿主细胞和包含cryET29 DNA片段的转基因植物的核酸片段。 公开了制备和使用所公开的蛋白质和核酸片段的方法以及用于在体内和体外检测cryET29和CryET29序列的测定和诊断试剂盒。
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5.发明授权Bacillus thuringiensis cryET4 and cryET5 protein insecticidal composition and method of use 失效苏云金芽孢杆菌cryET4和cryET5蛋白杀虫组合物及其使用方法
公开(公告)号：US5679343A
公开(公告)日：1997-10-21
申请号：US474038
申请日：1995-06-07
Applicant: William P. Donovan , Yuping Tan , Christine S. Jany , Jose M. Gonzalez, Jr.
Inventor： William P. Donovan , Yuping Tan , Christine S. Jany , Jose M. Gonzalez, Jr.
CPC classification number: C07K14/32 , C07K14/325 , C12R1/075 , Y10S435/832
Abstract: A Bacillus thuringiensis strain isolate, designated B.t. strain EG5847, exhibits insecticidal activity against lepidopteran insects. Two novel toxin genes from B.t. strain EG5847 designated cryET4 and cryET5 produce insecticidal proteins with activity against a broad spectrum of insects of the order Lepidoptera. The cryET4 gene has a nucleotide base sequence shown in FIG. 1 and listed in SEQ ID NO:1 and produces a CryET4 gene product having the deduced amino acid sequence shown in FIG. 1 and listed in SEQ ID NO:2. The cryET5 gene has a nucleotide base sequence shown in FIG. 2 and listed in SEQ ID NO:3 and produces a CryET5 gene product having the deduced amino acid sequence shown in FIG. 2 and listed in SEQ ID NO:4.
Abstract translation: 苏云金芽孢杆菌菌株分离株，命名为B.t. 菌株EG5847对鳞翅目昆虫表现出杀虫活性。 来自B.t.的两种新型毒素基因 命名为cryET4和cryET5的菌株EG5847产生具有针对鳞翅目鳞翅目昆虫的活性的杀虫蛋白。 cryET4基因具有图1所示的核苷酸碱基序列。 1并列于SEQ ID NO：1中，并产生具有如图1所示的推导的氨基酸序列的CryET4基因产物。 1并列于SEQ ID NO：2。 cryET5基因具有图1所示的核苷酸碱基序列。 2并列于SEQ ID NO：3中，并产生具有如图1所示的推导的氨基酸序列的CryET5基因产物。 2并列于SEQ ID NO：4。
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Patent Agency Ranking6.发明授权Bacillus thuringiensis cryet4 and cryet5 toxin genes and proteins toxic to lepidopteran insects 失效苏云金芽孢杆菌cryet4和cryet5毒素基因和对鳞翅目昆虫有毒的蛋白质
公开(公告)号：US5322687A
公开(公告)日：1994-06-21
申请号：US100709
申请日：1993-07-29
Applicant: William P. Donovan , Yuping Tan , Christine S. Jany , Jose M. Gonzalez, Jr.
Inventor： William P. Donovan , Yuping Tan , Christine S. Jany , Jose M. Gonzalez, Jr.
CPC classification number: C07K14/32 , C07K14/325 , C12R1/075 , Y10S435/832
Abstract: A Bacillus thuringiensis strain isolate, designated B.t. strain EG5847, exhibits insecticidal activity against lepidopteran insects. Two novel toxin genes from B.t. strain EG5847 designated cryET4 and cryET5 produce insecticidal proteins with activity against a broad spectrum of insects of the order Lepidoptera. The cryET4 gene has a nucleotide base sequence shown in FIG. 1 and listed in SEQ ID NO:1 and produces a CryET4 gene product having the deduced amino acid sequence shown in FIG. 1 and listed in SEQ ID NO:2. The cryET5 gene has a nucleotide base sequence shown in FIG. 2 and listed in SEQ ID NO:3 and produces a CryET5 gene product having the deduced amino acid sequence shown in FIG. 2 and listed in SEQ ID NO:4.
Abstract translation: 苏云金芽孢杆菌菌株分离株，命名为B.t. 菌株EG5847对鳞翅目昆虫表现出杀虫活性。 来自B.t.的两种新型毒素基因 命名为cryET4和cryET5的菌株EG5847产生具有针对鳞翅目鳞翅目昆虫的活性的杀虫蛋白。 cryET4基因具有图1所示的核苷酸碱基序列。 1并列于SEQ ID NO：1中，并产生具有如图1所示的推导的氨基酸序列的CryET4基因产物。 1并列于SEQ ID NO：2。 cryET5基因具有图1所示的核苷酸碱基序列。 2并列于SEQ ID NO：3中，并产生具有如图1所示的推导的氨基酸序列的CryET5基因产物。 2并列于SEQ ID NO：4。
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7.发明授权公开(公告)号：US06537756B1
公开(公告)日：2003-03-25
申请号：US09611216
申请日：2000-07-06
Applicant: Mark J. Rupar , William P. Donovan , Yuping Tan , Annette C. Slaney
Inventor： Mark J. Rupar , William P. Donovan , Yuping Tan , Annette C. Slaney
IPC: C12N1532
CPC classification number: A01N63/00 , C07K14/325 , C12N15/8286 , G01N2430/10 , Y02A40/162 , Y10S530/825
Abstract: Disclosed is a novel &dgr;-endotoxin, designated CryET29, that exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea (Ctenocephalides felis), as well as against coleopteran insects, including the southern corn rootworm (Diabrotica undecimpunctata), western corn rootworm (D. virgifera), Colorado potato beetle (Leptinotarsa decemlineata), Japanese beetle (Popillia japonica), and red flour beetle (Tribolium castaneum). Also disclosed are nucleic acid segments encoding CryET29, recombinant vectors, host cells, and transgenic plants comprising a cryET29 DNA segment. Methods for making and using the disclosed protein and nucleic acid segments are disclosed as well as assays and diagnostic kits for detecting cryET29 and CryET29 sequences in vivo and in vitro.
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8.发明授权公开(公告)号：US06468748B1
公开(公告)日：2002-10-22
申请号：US09515277
申请日：2000-02-29
Applicant: Joseph A. Monforte , Thomas A. Shaler , Yuping Tan , Christopher H. Becker
Inventor： Joseph A. Monforte , Thomas A. Shaler , Yuping Tan , Christopher H. Becker
IPC: C12Q168
CPC classification number: C12Q1/6827 , C12Q1/683 , C12Q2565/519 , C12Q2563/167 , C12Q2565/518 , C12Q2523/107 , C12Q2565/607 , C12Q2521/307
Abstract: This invention relates to methods for screening nucleic acids for mutations by analyzing nonrandomly fragmented nucleic acids using mass spectrometric techniques and to procedures for improving mass resolution and mass accuracy of these methods of detecting mutations.
Abstract translation: 本发明涉及通过使用质谱技术分析非随机碎片化核酸来筛选核酸的突变的方法，以及用于提高这些检测突变方法的质量分辨率和质量精度的方法。
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公开(公告)号：US07442540B2
公开(公告)日：2008-10-28
申请号：US11485079
申请日：2006-07-12
Applicant: Mark J. Rupar , William P. Donovan , Chih-Rei Chu , Elizabeth Pease , Yuping Tan , Annette C. Slaney , Thomas M. Malvar , James A. Baum
Inventor： Mark J. Rupar , William P. Donovan , Chih-Rei Chu , Elizabeth Pease , Yuping Tan , Annette C. Slaney , Thomas M. Malvar , James A. Baum
IPC: C12N15/32 , C12N1/21 , C07K14/325 , C12N15/82
CPC classification number: C07K14/325 , A01N63/02 , C12N15/8286 , Y02A40/162
Abstract: Disclosed are novel insecticidal polypeptides, and compositions comprising these polypeptides, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that contain nucleic acid segments that encode the disclosed δ-endotoxin polypeptides. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising these polynucleotide sequences, as well as methods for controlling an insect population, such as Colorado potato beetle, southern corn rootworm and western corn rootworm, and for conferring to a plant resistance to a target insect species.
Abstract translation: 公开了新型杀虫多肽，以及包含这些多肽，其肽片段和特异性抗体的组合物。 还公开了载体，转化的宿主细胞和含有编码公开的δ-内毒素多肽的核酸片段的转基因植物。 还公开了鉴定相关多肽和多核苷酸的方法，制备和使用包含这些多核苷酸序列的转基因细胞的方法，以及用于控制昆虫种群的方法，例如科罗拉多马铃薯甲虫，南方玉米根虫和西方玉米根虫，并赋予 对植物对目标昆虫物种的抗性。
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公开(公告)号：US07186893B2
公开(公告)日：2007-03-06
申请号：US10767605
申请日：2004-01-29
Applicant: Mark J. Rupar , William P. Donovan , Yuping Tan , Annette C. Slaney
Inventor： Mark J. Rupar , William P. Donovan , Yuping Tan , Annette C. Slaney
CPC classification number: A01N63/00 , C07K14/325 , C12N15/8286 , G01N2430/10 , Y02A40/162 , Y10S530/825
Abstract: Disclosed are transgenic plants, seeds, and progeny from such plants comprising a cryET29 DNA segment. The cryET29 DNA segment encodes CryET29 crystal protein which comprises SEQ ID NO:2 and exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea (Ctenocephalides felis), as well as against coleopteran insects, including the southern corn rootworm (Diabrotica undecimpunctata), western corn rootworm (D. virgifera), Colorado potato beetle (Leptinotarsa decemlineata), Japanese beetle (Popillia japonica), and red flour beetle (Tribolium castaneum).
Abstract translation: 公开了包含cryET29 DNA片段的来自这种植物的转基因植物，种子和后代。 cryET29 DNA区段编码CryET29晶体蛋白，其包含SEQ ID NO：2，并且显示出对iphonapteran昆虫的杀虫活性，包括猫跳蚤幼虫（Ctenocephalides felis）以及鞘翅目昆虫，包括南方玉米根虫（Diabrotica undecimpunctata） ，西方玉米根虫（D. virgifera），科罗拉多马铃薯甲虫（Leptinotarsa decemlineata），日本甲虫（Popillia japonica）和红粉甲虫（Tribolium castaneum））。
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