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    Novel Hemipteran and Coleopteran Active Toxin Proteins From Bacillus Thuringiensis
    1.
    发明申请
    Novel Hemipteran and Coleopteran Active Toxin Proteins From Bacillus Thuringiensis 有权
    来自苏云金芽孢杆菌的新型半翅目和鞘翅目活性毒素蛋白

    公开(公告)号：US20130305416A1

    公开(公告)日：2013-11-14

    申请号：US13950439

    申请日：2013-07-25

    申请人： James A. Baum ,  Stanislaw Flasinski ,  Gregory R. Heck ,  Stephen R. Penn ,  Uma Rao Sukuru ,  Xiaohong Shi

    发明人： James A. Baum ,  Stanislaw Flasinski ,  Gregory R. Heck ,  Stephen R. Penn ,  Uma Rao Sukuru ,  Xiaohong Shi

    IPC分类号： C07K14/325

    CPC分类号： C07K14/325 ,  A01N63/00 ,  C12N15/8286 ,  Y02A40/162

    摘要： A novel Bacillus thuringiensis crystal protein exhibiting insect inhibitory activity is disclosed. Growth of Lygus insects is significantly inhibited by providing the novel crystal protein in Lygus insect diet. Polynucleotides encoding the crystal protein, transgenic plants and microorganisms that contain the polynucleotides, isolated peptides derived from the crystal protein, and antibodies directed against the crystal protein are also provided. Methods of using the crystal protein and polynucleotides encoding the crystal protein to control Hemipteran insects are also disclosed.

    摘要翻译： 公开了一种具有昆虫抑制活性的新型苏云金芽孢杆菌晶体蛋白。 通过在us虫昆虫饮食中提供新的晶体蛋白，可以显着抑制us虫的生长。 还提供了编码结晶蛋白的多核苷酸，含有多核苷酸的转基因植物和微生物，从晶体蛋白衍生的分离的肽，以及针对结晶蛋白的抗体。 还公开了使用晶体蛋白质和编码晶体蛋白质的多核苷酸来控制半翅目昆虫的方法。

    CRY1C polypeptides having improved toxicity to lepidopteran insects
    5.
    发明授权
    CRY1C polypeptides having improved toxicity to lepidopteran insects 有权
    CRY1C多肽对鳞翅目昆虫具有改善的毒性

    公开(公告)号：US6033874A

    公开(公告)日：2000-03-07

    申请号：US314093

    申请日：1999-05-18

    申请人： James A. Baum ,  Amy Jelen Gilmer ,  Ann-Marie Light Mettus

    发明人： James A. Baum ,  Amy Jelen Gilmer ,  Ann-Marie Light Mettus

    IPC分类号： A01H5/00 ,  A01N63/00 ,  A01N63/02 ,  C07K14/325 ,  C07K16/12 ,  C12N1/21 ,  C12N15/09 ,  C12N15/31 ,  C12N15/82 ,  C12P21/02 ,  C12P21/08 ,  C12R1/38 ,  C12P21/06 ,  C07H17/00 ,  C07K14/00

    CPC分类号： C12R1/075 ,  C07K14/325 ,  C12N15/8286

    摘要： Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding .delta.-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the .alpha. helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.

    摘要翻译： 公开了编码对鳞翅目昆虫具有杀虫活性的δ-内毒素的新型合成修饰的苏云金芽孢杆菌核酸片段。 还公开了由这些新型核酸序列编码的合成晶体蛋白质。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。 还公开了用于修饰，改变和诱变这些晶体蛋白（包括Cry1C）的结构域1中的α螺旋之间的特异性环区的方法，以产生遗传工程改造的重组cry *基因及其编码的具有改善的杀虫活性的蛋白质。 在优选的实施方案中，公开了新的Cry1C *氨基酸片段和编码它们的经修饰的cry1C *核酸序列。

    Insecticidal compositions and methods for making insect-resistant transgenic plants
    7.
    发明授权
    Insecticidal compositions and methods for making insect-resistant transgenic plants 有权
    杀虫组合物和制备抗虫转基因植物的方法

    公开(公告)号：US09121035B2

    公开(公告)日：2015-09-01

    申请号：US12064840

    申请日：2006-08-30

    申请人： James A. Baum ,  James K. Roberts ,  Bei Zhang ,  Heather Anderson ,  Catherine A. Chay

    发明人： James A. Baum ,  James K. Roberts ,  Bei Zhang ,  Heather Anderson ,  Catherine A. Chay

    IPC分类号： C12N15/82 ,  C07K14/325 ,  A01N63/02

    CPC分类号： C12N15/8286 ,  A01N63/02 ,  C07K14/325 ,  C12N15/8216 ,  C12N15/8257 ,  Y02A40/162

    摘要： The present invention provides isolated polynucleotide sequences encoding ET37, TIC810 and TIC812 proteins from Bacillus thuringiensis, and nucleotide sequences for use in expressing TIC809, ET37, TIC810 and TIC812, and fusions of various insecticidally effective combinations of these proteins such as TIC 127, in plants. Methods of making and using the polynucleotide sequences and the proteins in the development of transgenic plant cells and transgenic plants exhibiting improved insect resistance against (1) Coleopteran insects including Western Corn Rootworm (Dibrotica virgifera), Southern Corn Rootworm (Dibrotica undecempunctata), Northern Corn Rootworm (Diabrotica barbed), Mexican Corn Rootworm (Diabrotica virgifera zeae), Brazilian Corn Rootworm (Diabrotica balteata) and Brazilian Corn Rootworm complex (Diabrotica viridula and Diabrotica speciosa), and against Hemipteran insects such as Lygus bugs, are disclosed.

    摘要翻译： 本发明提供编码来自苏云金芽孢杆菌的ET37，TIC810和TIC812蛋白质的分离的多核苷酸序列，以及用于表达TIC809，ET37，TIC810和TIC812的核苷酸序列，以及这些蛋白质如TIC 127在植物中的各种杀虫有效组合的融合 。 在转基因植物细胞和转基因植物的开发中制备和使用多核苷酸序列和蛋白质的方法和转基因植物对（1）鞘翅目昆虫包括西方玉米根虫（Dibrotica virgifera），南方玉米根虫（Dibrotica undecempunctata），北方玉米 披露了根虫（Diabrotica barbed），墨西哥玉米根虫（Diabrotica virgifera zeae），巴西玉米根虫（Diabrotica balteata）和巴西玉米Rootworm综合体（Diabrotica viridula和Diabrotica speciosa），以及针对半翅目昆虫如Lygus虫。