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公开(公告)号：US6033874A
公开(公告)日：2000-03-07
申请号：US314093
申请日：1999-05-18
IPC分类号： A01H5/00 , A01N63/00 , A01N63/02 , C07K14/325 , C07K16/12 , C12N1/21 , C12N15/09 , C12N15/31 , C12N15/82 , C12P21/02 , C12P21/08 , C12R1/38 , C12P21/06 , C07H17/00 , C07K14/00
CPC分类号： C12R1/075 , C07K14/325 , C12N15/8286
摘要： Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding .delta.-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the .alpha. helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.
摘要翻译： 公开了编码对鳞翅目昆虫具有杀虫活性的δ-内毒素的新型合成修饰的苏云金芽孢杆菌核酸片段。 还公开了由这些新型核酸序列编码的合成晶体蛋白质。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。 还公开了用于修饰，改变和诱变这些晶体蛋白（包括Cry1C）的结构域1中的α螺旋之间的特异性环区的方法，以产生遗传工程改造的重组cry *基因及其编码的具有改善的杀虫活性的蛋白质。 在优选的实施方案中，公开了新的Cry1C *氨基酸片段和编码它们的经修饰的cry1C *核酸序列。
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公开(公告)号：US5965428A
公开(公告)日：1999-10-12
申请号：US731079
申请日：1996-10-08
申请人： Amy Jelen Gilmer , James A. Baum
发明人： Amy Jelen Gilmer , James A. Baum
IPC分类号： C07K14/325 , C12N1/20 , C07K1/00
CPC分类号： C07K14/325 , C07K2319/00
摘要： The present invention is directed to a chimeric crystal protein comprising CryIF and CryIAc. Bacillus thuringiensis isolates comprising said protein and a method of controlling lepidopterans are also included in the present invention.
摘要翻译： 本发明涉及包含CryIF和CryIAc的嵌合晶体蛋白质。 包含所述蛋白质的苏云金芽孢杆菌分离物和控制鳞翅目昆虫的方法也包括在本发明中。
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3.发明授权Nuclei acid and polypeptide compositions encoding lepidopteran-toxic polypeptides 有权编码鳞翅目昆虫毒性多肽的核酸和多肽组合物公开(公告)号：US06423828B1
公开(公告)日：2002-07-23
申请号：US09337280
申请日：1999-06-22
IPC分类号： C07K1400
CPC分类号： C12R1/075 , C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding &dgr;-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the &agr; helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.
摘要翻译： 公开了编码对鳞翅目昆虫具有杀虫活性的δ-内毒素的新型合成修饰的苏云金芽孢杆菌核酸片段。 还公开了由这些新型核酸序列编码的合成晶体蛋白质。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。 还公开了用于修饰，改变和诱变这些晶体蛋白（包括Cry1C）的结构域1中的α螺旋之间的特异性环区的方法，以产生遗传工程改造的重组cry *基因及其编码的具有改善的杀虫活性的蛋白质。 在优选的实施方案中，公开了新的Cry1C *氨基酸片段和编码它们的经修饰的cry1C *核酸序列。
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4.发明授权Polypeptide compositions toxic to lepidopteran insects and methods for making same 有权对鳞翅目昆虫有毒的多肽组合物及其制备方法
公开(公告)号：US6153814A
公开(公告)日：2000-11-28
申请号：US250848
申请日：1999-02-17
IPC分类号： A01H5/00 , A01N63/00 , A01N63/02 , C07K14/325 , C07K16/12 , C12N1/21 , C12N15/09 , C12N15/31 , C12N15/82 , C12P21/02 , C12P21/08 , C12R1/38 , A01H1/04 , C12P21/06 , C07H17/00 , C07K14/00
CPC分类号： C12R1/075 , C07K14/325 , C12N15/8286
摘要： Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding .delta.-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the .alpha. helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.
摘要翻译： 公开了编码对鳞翅目昆虫具有杀虫活性的δ-内毒素的新型合成修饰的苏云金芽孢杆菌核酸片段。 还公开了由这些新型核酸序列编码的合成晶体蛋白质。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。 还公开了用于修饰，改变和诱变这些晶体蛋白（包括Cry1C）的结构域1中的α螺旋之间的特异性环区的方法，以产生遗传工程改造的重组cry *基因及其编码的具有改善的杀虫活性的蛋白质。 在优选的实施方案中，公开了新的Cry1C *氨基酸片段和编码它们的经修饰的cry1C *核酸序列。
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公开(公告)号：US07256017B2
公开(公告)日：2007-08-14
申请号：US10999188
申请日：2004-11-29
CPC分类号： C12R1/075 , C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding δ-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the α helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.
摘要翻译： 公开了编码对鳞翅目昆虫具有杀虫活性的δ-内毒素的新型合成修饰的苏云金芽孢杆菌核酸片段。 还公开了由这些新型核酸序列编码的合成晶体蛋白质。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。 还公开了用于修饰，改变和诱变这些晶体蛋白（包括Cry1C）的结构域1中的α螺旋之间的特异性环区的方法，以产生遗传工程改造的重组cry *基因及其编码的具有改善的杀虫活性的蛋白质。 在优选的实施方案中，公开了新的Cry1C *氨基酸片段和编码它们的经修饰的cry1C *核酸序列。
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6.发明授权Nucleic acid and polypeptide compositions encoding lepidopteran-toxic polypeptides 有权编码鳞翅目昆虫毒性多肽的核酸和多肽组合物公开(公告)号：US06825006B2
公开(公告)日：2004-11-30
申请号：US10200522
申请日：2002-07-22
IPC分类号： C12P2106
CPC分类号： C12R1/075 , C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding &dgr;-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the &agr; helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.
摘要翻译： 公开了编码对鳞翅目昆虫具有杀虫活性的δ-内毒素的新型合成修饰的苏云金芽孢杆菌核酸片段。 还公开了由这些新型核酸序列编码的合成晶体蛋白质。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。 还公开了用于修饰，改变和诱变这些晶体蛋白（包括Cry1C）的结构域1中的α螺旋之间的特异性环区的方法，以产生遗传工程改造的重组cry *基因及其编码的具有改善的杀虫活性的蛋白质。 在优选的实施方案中，公开了新的Cry1C *氨基酸片段和编码它们的经修饰的cry1C *核酸序列。
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7.发明授权公开(公告)号：US06809078B2
公开(公告)日：2004-10-26
申请号：US09972175
申请日：2001-10-05
IPC分类号： A61K3800
CPC分类号： C12R1/075 , C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding &dgr;-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the &agr; helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.
摘要翻译： 公开了编码对鳞翅目昆虫具有杀虫活性的δ-内毒素的新型合成修饰的苏云金芽孢杆菌核酸片段。 还公开了由这些新型核酸序列编码的合成晶体蛋白质。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。 还公开了用于修饰，改变和诱变这些晶体蛋白（包括Cry1C）的结构域1中的α螺旋之间的特异性环区的方法，以产生遗传工程改造的重组cry *基因及其编码的具有改善的杀虫活性的蛋白质。 在优选的实施方案中，公开了新的Cry1C *氨基酸片段和编码它们的经修饰的cry1C *核酸序列。
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公开(公告)号：US06313378B1
公开(公告)日：2001-11-06
申请号：US09337635
申请日：1999-06-21
IPC分类号： A01H500
CPC分类号： C12R1/075 , C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding &dgr;-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the &agr; helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.
摘要翻译： 公开了编码对鳞翅目昆虫具有杀虫活性的δ-内毒素的新型合成修饰的苏云金芽孢杆菌核酸片段。 还公开了由这些新型核酸序列编码的合成晶体蛋白质。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。 还公开了用于修饰，改变和诱变这些晶体蛋白（包括Cry1C）的结构域1中的α螺旋之间的特异性环区的方法，以产生遗传工程改造的重组cry *基因及其编码的具有改善的杀虫活性的蛋白质。 在优选的实施方案中，公开了新的Cry1C *氨基酸片段和编码它们的经修饰的cry1C *核酸序列。
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公开(公告)号：US5914318A
公开(公告)日：1999-06-22
申请号：US980071
申请日：1997-11-26
IPC分类号： A01H5/00 , A01N63/00 , A01N63/02 , C07K14/325 , C07K16/12 , C12N1/21 , C12N15/09 , C12N15/31 , C12N15/82 , C12P21/02 , C12P21/08 , C12R1/38
CPC分类号： C12R1/075 , C07K14/325 , C12N15/8286
摘要： Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding .delta.-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the .alpha. helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.
摘要翻译： 公开了编码对鳞翅目昆虫具有杀虫活性的δ-内毒素的新型合成修饰的苏云金芽孢杆菌核酸片段。 还公开了由这些新型核酸序列编码的合成晶体蛋白质。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。 还公开了用于修饰，改变和诱变这些晶体蛋白（包括Cry1C）的结构域1中的α螺旋之间的特异性环区的方法，以产生遗传工程改造的重组cry *基因及其编码的具有改善的杀虫活性的蛋白质。 在优选的实施方案中，公开了新的Cry1C *氨基酸片段和编码它们的经修饰的cry1C *核酸序列。
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10.发明授权Bacillus thuringiensis Cry1C compositions toxic to lepidopteran insects and methods for making Cry1C mutants 失效苏云金芽孢杆菌Cry1C组成对鳞翅目昆虫的毒性和Cry1C突变体的制备方法
公开(公告)号：US5942664A
公开(公告)日：1999-08-24
申请号：US757536
申请日：1996-11-27
IPC分类号： A01H5/00 , A01N63/00 , A01N63/02 , C07K14/325 , C07K16/12 , C12N1/21 , C12N15/09 , C12N15/31 , C12N15/82 , C12P21/02 , C12P21/08 , C12R1/38 , A01H1/04 , C12P21/06 , C07H17/00 , C07K14/00
CPC分类号： C12R1/075 , C07K14/325 , C12N15/8286
摘要： Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding .delta.-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the .alpha. helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.
摘要翻译： 公开了编码对鳞翅目昆虫具有杀虫活性的δ-内毒素的新型合成修饰的苏云金芽孢杆菌核酸片段。 还公开了由这些新型核酸序列编码的合成晶体蛋白质。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。 还公开了用于修饰，改变和诱变这些晶体蛋白（包括Cry1C）的结构域1中的α螺旋之间的特异性环区的方法，以产生遗传工程改造的重组cry *基因及其编码的具有改善的杀虫活性的蛋白质。 在优选的实施方案中，公开了新的Cry1C *氨基酸片段和编码它们的经修饰的cry1C *核酸序列。
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