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1.发明授权Bacillus thuringiensis Cry1C compositions toxic to lepidopteran insects and methods for making Cry1C mutants 失效苏云金芽孢杆菌Cry1C组成对鳞翅目昆虫的毒性和Cry1C突变体的制备方法
公开(公告)号：US5942664A
公开(公告)日：1999-08-24
申请号：US757536
申请日：1996-11-27
IPC分类号： A01H5/00 , A01N63/00 , A01N63/02 , C07K14/325 , C07K16/12 , C12N1/21 , C12N15/09 , C12N15/31 , C12N15/82 , C12P21/02 , C12P21/08 , C12R1/38 , A01H1/04 , C12P21/06 , C07H17/00 , C07K14/00
CPC分类号： C12R1/075 , C07K14/325 , C12N15/8286
摘要： Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding .delta.-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the .alpha. helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.
摘要翻译： 公开了编码对鳞翅目昆虫具有杀虫活性的δ-内毒素的新型合成修饰的苏云金芽孢杆菌核酸片段。 还公开了由这些新型核酸序列编码的合成晶体蛋白质。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。 还公开了用于修饰，改变和诱变这些晶体蛋白（包括Cry1C）的结构域1中的α螺旋之间的特异性环区的方法，以产生遗传工程改造的重组cry *基因及其编码的具有改善的杀虫活性的蛋白质。 在优选的实施方案中，公开了新的Cry1C *氨基酸片段和编码它们的经修饰的cry1C *核酸序列。
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2.发明授权Nuclei acid and polypeptide compositions encoding lepidopteran-toxic polypeptides 有权编码鳞翅目昆虫毒性多肽的核酸和多肽组合物公开(公告)号：US06423828B1
公开(公告)日：2002-07-23
申请号：US09337280
申请日：1999-06-22
IPC分类号： C07K1400
CPC分类号： C12R1/075 , C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding &dgr;-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the &agr; helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.
摘要翻译： 公开了编码对鳞翅目昆虫具有杀虫活性的δ-内毒素的新型合成修饰的苏云金芽孢杆菌核酸片段。 还公开了由这些新型核酸序列编码的合成晶体蛋白质。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。 还公开了用于修饰，改变和诱变这些晶体蛋白（包括Cry1C）的结构域1中的α螺旋之间的特异性环区的方法，以产生遗传工程改造的重组cry *基因及其编码的具有改善的杀虫活性的蛋白质。 在优选的实施方案中，公开了新的Cry1C *氨基酸片段和编码它们的经修饰的cry1C *核酸序列。
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3.发明授权Polypeptide compositions toxic to lepidopteran insects and methods for making same 有权对鳞翅目昆虫有毒的多肽组合物及其制备方法
公开(公告)号：US6153814A
公开(公告)日：2000-11-28
申请号：US250848
申请日：1999-02-17
IPC分类号： A01H5/00 , A01N63/00 , A01N63/02 , C07K14/325 , C07K16/12 , C12N1/21 , C12N15/09 , C12N15/31 , C12N15/82 , C12P21/02 , C12P21/08 , C12R1/38 , A01H1/04 , C12P21/06 , C07H17/00 , C07K14/00
CPC分类号： C12R1/075 , C07K14/325 , C12N15/8286
摘要： Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding .delta.-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the .alpha. helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.
摘要翻译： 公开了编码对鳞翅目昆虫具有杀虫活性的δ-内毒素的新型合成修饰的苏云金芽孢杆菌核酸片段。 还公开了由这些新型核酸序列编码的合成晶体蛋白质。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。 还公开了用于修饰，改变和诱变这些晶体蛋白（包括Cry1C）的结构域1中的α螺旋之间的特异性环区的方法，以产生遗传工程改造的重组cry *基因及其编码的具有改善的杀虫活性的蛋白质。 在优选的实施方案中，公开了新的Cry1C *氨基酸片段和编码它们的经修饰的cry1C *核酸序列。
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公开(公告)号：US07256017B2
公开(公告)日：2007-08-14
申请号：US10999188
申请日：2004-11-29
CPC分类号： C12R1/075 , C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding δ-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the α helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.
摘要翻译： 公开了编码对鳞翅目昆虫具有杀虫活性的δ-内毒素的新型合成修饰的苏云金芽孢杆菌核酸片段。 还公开了由这些新型核酸序列编码的合成晶体蛋白质。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。 还公开了用于修饰，改变和诱变这些晶体蛋白（包括Cry1C）的结构域1中的α螺旋之间的特异性环区的方法，以产生遗传工程改造的重组cry *基因及其编码的具有改善的杀虫活性的蛋白质。 在优选的实施方案中，公开了新的Cry1C *氨基酸片段和编码它们的经修饰的cry1C *核酸序列。
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5.发明授权Nucleic acid and polypeptide compositions encoding lepidopteran-toxic polypeptides 有权编码鳞翅目昆虫毒性多肽的核酸和多肽组合物公开(公告)号：US06825006B2
公开(公告)日：2004-11-30
申请号：US10200522
申请日：2002-07-22
IPC分类号： C12P2106
CPC分类号： C12R1/075 , C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding &dgr;-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the &agr; helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.
摘要翻译： 公开了编码对鳞翅目昆虫具有杀虫活性的δ-内毒素的新型合成修饰的苏云金芽孢杆菌核酸片段。 还公开了由这些新型核酸序列编码的合成晶体蛋白质。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。 还公开了用于修饰，改变和诱变这些晶体蛋白（包括Cry1C）的结构域1中的α螺旋之间的特异性环区的方法，以产生遗传工程改造的重组cry *基因及其编码的具有改善的杀虫活性的蛋白质。 在优选的实施方案中，公开了新的Cry1C *氨基酸片段和编码它们的经修饰的cry1C *核酸序列。
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6.发明授权公开(公告)号：US06809078B2
公开(公告)日：2004-10-26
申请号：US09972175
申请日：2001-10-05
IPC分类号： A61K3800
CPC分类号： C12R1/075 , C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding &dgr;-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the &agr; helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.
摘要翻译： 公开了编码对鳞翅目昆虫具有杀虫活性的δ-内毒素的新型合成修饰的苏云金芽孢杆菌核酸片段。 还公开了由这些新型核酸序列编码的合成晶体蛋白质。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。 还公开了用于修饰，改变和诱变这些晶体蛋白（包括Cry1C）的结构域1中的α螺旋之间的特异性环区的方法，以产生遗传工程改造的重组cry *基因及其编码的具有改善的杀虫活性的蛋白质。 在优选的实施方案中，公开了新的Cry1C *氨基酸片段和编码它们的经修饰的cry1C *核酸序列。
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公开(公告)号：US06313378B1
公开(公告)日：2001-11-06
申请号：US09337635
申请日：1999-06-21
IPC分类号： A01H500
CPC分类号： C12R1/075 , C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding &dgr;-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the &agr; helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.
摘要翻译： 公开了编码对鳞翅目昆虫具有杀虫活性的δ-内毒素的新型合成修饰的苏云金芽孢杆菌核酸片段。 还公开了由这些新型核酸序列编码的合成晶体蛋白质。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。 还公开了用于修饰，改变和诱变这些晶体蛋白（包括Cry1C）的结构域1中的α螺旋之间的特异性环区的方法，以产生遗传工程改造的重组cry *基因及其编码的具有改善的杀虫活性的蛋白质。 在优选的实施方案中，公开了新的Cry1C *氨基酸片段和编码它们的经修饰的cry1C *核酸序列。
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公开(公告)号：US5914318A
公开(公告)日：1999-06-22
申请号：US980071
申请日：1997-11-26
IPC分类号： A01H5/00 , A01N63/00 , A01N63/02 , C07K14/325 , C07K16/12 , C12N1/21 , C12N15/09 , C12N15/31 , C12N15/82 , C12P21/02 , C12P21/08 , C12R1/38
CPC分类号： C12R1/075 , C07K14/325 , C12N15/8286
摘要： Disclosed are novel synthetically-modified B. thuringiensis nucleic acid segments encoding .delta.-endotoxins having insecticidal activity against lepidopteran insects. Also disclosed are synthetic crystal proteins encoded by these novel nucleic acid sequences. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention. Also disclosed are methods for modifying, altering, and mutagenizing specific loop regions between the .alpha. helices in domain 1 of these crystal proteins, including Cry1C, to produce genetically-engineered recombinant cry* genes, and the proteins they encode which have improved insecticidal activity. In preferred embodiments, novel Cry1C* amino acid segments and the modified cry1C* nucleic acid sequences which encode them are disclosed.
摘要翻译： 公开了编码对鳞翅目昆虫具有杀虫活性的δ-内毒素的新型合成修饰的苏云金芽孢杆菌核酸片段。 还公开了由这些新型核酸序列编码的合成晶体蛋白质。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。 还公开了用于修饰，改变和诱变这些晶体蛋白（包括Cry1C）的结构域1中的α螺旋之间的特异性环区的方法，以产生遗传工程改造的重组cry *基因及其编码的具有改善的杀虫活性的蛋白质。 在优选的实施方案中，公开了新的Cry1C *氨基酸片段和编码它们的经修饰的cry1C *核酸序列。
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公开(公告)号：US20090087863A1
公开(公告)日：2009-04-02
申请号：US12240110
申请日：2008-09-29
CPC分类号： C07K14/325 , C07K2319/00 , C12N15/8286 , Y02A40/162 , Y10S530/825
摘要： Disclosed is a novel Lepidopteran- and Coleopteran-active δ-endotoxin polypeptide, and compositions comprising the polypeptide, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that comprise nucleic acid segments encoding the polypeptide. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising the novel sequences of the invention, as well as methods for controlling an insect population, such as the Western Corn Rootworm and Colorado potato beetle, and for conferring to a plant population resistance to the target insect species.
摘要翻译： 公开了一种新型鳞翅目昆虫和鞘翅目昆虫活性δ-内毒素多肽，以及包含多肽，其肽片段和特异性抗体的组合物。 还公开了载体，转化的宿主细胞和包含编码多肽的核酸片段的转基因植物。 还公开了鉴定相关多肽和多核苷酸的方法，制备和使用包含本发明新颖序列的转基因细胞的方法，以及用于控制昆虫种群的方法，例如西部玉米根虫和科罗拉多马铃薯甲虫，以及赋予 对植物群体抵抗目标昆虫物种。
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10.发明授权DNA probes useful in detecting δ-endotoxin-encoding sequences and uses thereof 有权用于检测δ-内毒素编码序列的DNA探针及其用途公开(公告)号：US07429454B2
公开(公告)日：2008-09-30
申请号：US11332654
申请日：2006-01-13
CPC分类号： C07K14/325 , C07K2319/00 , C12N15/8286 , Y02A40/162 , Y10S530/825
摘要： Disclosed are novel DNA probes useful in detecting a nucleic acid sequence encoding a novel Lepidopteran- and Coleopteran-active δ-endotoxin polypeptide. Also disclosed are nucleic acid detection methods and kits involving the novel DNA probes.
摘要翻译： 公开了可用于检测编码新型鳞翅目昆虫和鞘翅目昆虫活性δ-内毒素多肽的核酸序列的新型DNA探针。 还公开了涉及新型DNA探针的核酸检测方法和试剂盒。
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