知搜-全球专利检索

  1. 登录
  2. 注册

搜索结果

26 条记录 (耗时 0.024 秒)

    Porous substrate plates and the use thereof
    1.
    发明授权
    Porous substrate plates and the use thereof 有权
    多孔基板及其用途

    公开(公告)号：US08129198B2

    公开(公告)日：2012-03-06

    申请号：US12854588

    申请日：2010-08-11

    申请人： Ye Fang ,  Ann M. Ferrie ,  Yulong Hong ,  Brian L. Webb

    发明人： Ye Fang ,  Ann M. Ferrie ,  Yulong Hong ,  Brian L. Webb

    IPC分类号： G01N33/543

    CPC分类号： B01L3/5085 ,  B01L2200/12 ,  B01L2300/069 ,  B01L2300/0829 ,  Y10S435/81 ,  Y10S436/809 ,  Y10T29/49885

    摘要： A substrate plate or device adapted for use with biological or chemical assays is disclosed. The device may take the form of a multi-well plate having a three-dimensional, porous layer as part of a support surface within each well for immobilizing probe species. The porous layer is characterized as having a plurality of interconnected voids defined by a matrix of contiguous solid material. A method and its variants are also described.

    摘要翻译： 公开了适用于生物或化学测定的底物板或装置。 该装置可以采用具有三维多孔层的多孔板的形式，作为每个孔内的支撑表面的一部分，用于固定探针物质。 多孔层的特征在于具有由连续的固体材料的基体限定的多个相互连接的空隙。 还描述了一种方法及其变体。

    Porous substrate plates and the use thereof
    2.
    发明授权
    Porous substrate plates and the use thereof 失效
    多孔基板及其用途

    公开(公告)号：US07384779B2

    公开(公告)日：2008-06-10

    申请号：US10822385

    申请日：2004-04-12

    申请人： Ye Fang ,  Ann M. Ferrie ,  Yulong Hong ,  Brian L. Webb

    发明人： Ye Fang ,  Ann M. Ferrie ,  Yulong Hong ,  Brian L. Webb

    IPC分类号： C12M1/34

    CPC分类号： B01L3/5085 ,  B01L2200/12 ,  B01L2300/069 ,  B01L2300/0829 ,  Y10S435/81 ,  Y10S436/809 ,  Y10T29/49885

    摘要： A substrate plate or device adapted for use with biological or chemical assays is disclosed. The device may take the form of a multi-well plate having a three-dimensional, porous layer as part of a support surface within each well for immobilizing probe species. The porous layer is characterized as having a plurality of interconnected voids defined by a matrix of contiguous solid material. A method and its variants are also described.

    摘要翻译： 公开了适用于生物或化学测定的底物板或装置。 该装置可以采用具有三维多孔层的多孔板的形式，作为每个孔内的支撑表面的一部分，用于固定探针物质。 多孔层的特征在于具有由连续的固体材料的基体限定的多个相互连接的空隙。 还描述了一种方法及其变体。

    POROUS SUBSTRATE PLATES AND THE USE THEREOF
    3.
    发明申请
    POROUS SUBSTRATE PLATES AND THE USE THEREOF 失效
    多孔基板及其使用

    公开(公告)号：US20080119370A1

    公开(公告)日：2008-05-22

    申请号：US11870742

    申请日：2007-10-11

    申请人： Ye Fang ,  Ann M. Ferrie ,  Yulong Hong ,  Brian L. Webb

    发明人： Ye Fang ,  Ann M. Ferrie ,  Yulong Hong ,  Brian L. Webb

    IPC分类号： C40B30/04 ,  C40B50/18

    CPC分类号： B01L3/5085 ,  B01L2200/12 ,  B01L2300/069 ,  B01L2300/0829 ,  Y10S435/81 ,  Y10S436/809 ,  Y10T29/49885

    摘要： A substrate plate or device adapted for use with biological or chemical assays is disclosed. The device may take the form of a multi-well plate having a three-dimensional, porous layer as part of a support surface within each well for immobilizing probe species. The porous layer is characterized as having a plurality of interconnected voids defined by a matrix of contiguous solid material. A method and its variants are also described.

    摘要翻译： 公开了适用于生物或化学测定的底物板或装置。 该装置可以采用具有三维多孔层的多孔板的形式，作为每个孔内的支撑表面的一部分，用于固定探针物质。 多孔层的特征在于具有由连续的固体材料的基体限定的多个相互连接的空隙。 还描述了一种方法及其变体。

    Fluorescent ligands for GPCR arrays
    5.
    发明申请
    Fluorescent ligands for GPCR arrays 审中-公开
    GPCR阵列的荧光配体

    公开(公告)号：US20050009205A1

    公开(公告)日：2005-01-13

    申请号：US10741213

    申请日：2003-12-19

    申请人： Ye Fang ,  Yulong Hong ,  Jinlin Peng

    发明人： Ye Fang ,  Yulong Hong ,  Jinlin Peng

    IPC分类号： C07K7/08 ,  C07K14/63 ,  G01N33/543

    CPC分类号： G01N33/582 ,  C07K7/083 ,  C07K14/63 ,  G01N33/6845 ,  G01N33/74 ,  G01N2333/63 ,  G01N2333/726

    摘要： A fluorescent ligand includes a material having a binding affinity in the range of about 0.01 to about 25 nM, or about 0.1 to about 10 nM; a specificity to its cognate receptor in the range of about 50 to about 99%, or about 65 to about 99%; a cross-activity to other receptors of 0 to about 20%, or 0 to about 10%; a net charge per ligand of about −3 to about +5, or more preferably, about −2 to about +2 or most preferably for small compound ligands about −1 to about +2. The ligand may also have a hydrophobicity in the range of about 3 to about 55 minutes eluting time (as measured under specified eluting conditions). In some embodiments, the ligand including fluorescently labeled motilin 1-16 labeled with Bodipy-TMR, rhodamine or Cy5-. Other embodiments include fluorescently labeled Cy5-naltrexone, Cy5-neurotensin 2-13, N-terminal labeled neurotensin 2-13 or lys-labeled labeled neurotensin 2-13.

    摘要翻译： 荧光配体包括具有约0.01至约25nM，或约0.1至约10nM范围内的结合亲和力的材料; 在约50至约99％，或约65至约99％的范围内对其同源受体的特异性; 与其他受体的交叉活性为0至约20％，或0至约10％; 每个配体的净电荷为约-3至约+5，或更优选约-2至约+2，或最优选约为-1至约+2的小化合物配体。 配体还可以具有约3至约55分钟洗脱时间（在规定的洗脱条件下测量）的疏水性。 在一些实施方案中，配体包括用Bodipy-TMR，罗丹明或Cy5-标记的荧光标记的胃动素1-16。 其他实施方案包括荧光标记的Cy5-纳曲酮，Cy5-神经丝氨酸2-13，N-末端标记的神经丝氨酸蛋白酶2-13或lys-标记的标记的神经丝氨酸素2-13。

    Assay solution compositions and methods for GPCR arrays
    7.
    发明授权
    Assay solution compositions and methods for GPCR arrays 失效
    测定GPCR阵列的溶液组成和方法

    公开(公告)号：US07816096B2

    公开(公告)日：2010-10-19

    申请号：US12697664

    申请日：2010-02-01

    申请人： Ye Fang ,  Ann M. Ferrie ,  Yulong Hong ,  Sadashiva K. Pai ,  Jinlin Peng ,  Brian L. Webb

    发明人： Ye Fang ,  Ann M. Ferrie ,  Yulong Hong ,  Sadashiva K. Pai ,  Jinlin Peng ,  Brian L. Webb

    IPC分类号： G01N33/53 ,  G01N33/543

    CPC分类号： G01N33/52 ,  G01N2333/726

    摘要： Buffered assay solutions for performing 1) binding or 2) functional assays on GPCR arrays, along with methods for their use are described. The buffered assay solution has an underlying composition having: a buffer reagent with a pH in the range of about 6.5 to about 7.9; an inorganic salt of either a monovalent or divalent species, at a concentration from about 1 mM to about 500 mM; and optionally a combination of: c) a blocker reagent at a concentration of about 0.01 wt. % to about 2 wt. % of the composition, or d) protease-inhibitor at a concentration of about 0.001 mM to about 100 mM. In an embodiment for functional assay uses, the composition is modified to also include a GTP-analogue, a guanosine 5′-diphosphate (GDP) salt, and/or an anti-oxidant reagent.

    摘要翻译： 描述了用于执行1）GPCR阵列上的结合或2）功能测定的缓冲测定溶液及其使用方法。 缓冲测定溶液具有下列组成，其具有：pH在约6.5至约7.9范围内的缓冲试剂; 浓度为约1mM至约500mM的单价或二价物质的无机盐; 和任选的以下组合：c）浓度为约0.01wt。 ％至约2wt。 ％的组合物，或d）浓度为约0.001mM至约100mM的蛋白酶抑制剂。 在功能测定用途的实施方案中，组合物被修饰为还包括GTP类似物，鸟苷5'-二磷酸（GDP）盐和/或抗氧化试剂。

    Assay solution compositions and methods for GPCR arrays
    10.
    发明申请
    Assay solution compositions and methods for GPCR arrays 失效
    测定GPCR阵列的溶液组成和方法

    公开(公告)号：US20060148006A1

    公开(公告)日：2006-07-06

    申请号：US11312776

    申请日：2005-12-21

    申请人： Ye Fang ,  Ann Ferrie ,  Yulong Hong ,  Sadashiva Pai ,  Jinlin Peng ,  Brian Webb

    发明人： Ye Fang ,  Ann Ferrie ,  Yulong Hong ,  Sadashiva Pai ,  Jinlin Peng ,  Brian Webb

    IPC分类号： G01N33/567 ,  G01N33/53

    CPC分类号： G01N33/52 ,  G01N2333/726

    摘要： Buffered assay solutions for performing 1) binding or 2) functional assays on GPCR arrays, along with methods for their use are described. The buffered assay solution has an underlying composition having: a buffer reagent with a pH in the range of about 6.5 to about 7.9; an inorganic salt of either a monovalent or divalent species, at a concentration from about 1 mM to about 500 mM; and optionally a combination of: c) a blocker reagent at a concentration of about 0.01 wt. % to about 2 wt. % of the composition, or d) protease-inhibitor at a concentration of about 0.001 mM to about 100 mM. In an embodiment for functional assay uses, the composition is modified to also include a GTP-analogue, a guanosine 5′-diphosphate (GDP) salt, and/or an anti-oxidant reagent.

    摘要翻译： 描述了用于执行1）GPCR阵列上的结合或2）功能测定的缓冲测定溶液及其使用方法。 缓冲测定溶液具有下列组成，其具有：pH在约6.5至约7.9范围内的缓冲试剂; 浓度为约1mM至约500mM的单价或二价物质的无机盐; 和任选的以下组合：c）浓度为约0.01wt。 ％至约2wt。 ％的组合物，或d）浓度为约0.001mM至约100mM的蛋白酶抑制剂。 在功能测定用途的实施方案中，组合物被修饰为还包括GTP类似物，鸟苷5'-二磷酸（GDP）盐和/或抗氧化试剂。