公开(公告)号：US06979817B2

公开(公告)日：2005-12-27

申请号：US10450608

申请日：2002-04-08

Applicant: Yoshio Yamauchi ,  Tohru Natsume ,  Toshiaki Isobe

Inventor： Yoshio Yamauchi ,  Tohru Natsume ,  Toshiaki Isobe

IPC: G01N27/62 ,  H01J49/04

CPC classification number: H01J49/0013 ,  H01J49/167

Abstract: A problem is to provide a microspray column capable of increasing the ionization efficiency, and a high sensitive mass spectrometer and a mass spectrometric method using such a microspray column. For solving this problem, we provide a column for introducing a sample in an ionization source of a mass spectrometer (ESI/MS) being designed to ionize a sample molecule with electrospray and introduce it into an analyzer, has structural features of: (1) 0.5 μm or less in an inner diameter of a tip opening of the column; (2) 0.5 μm or more and 5 μm or less in a particle size of a column filler; and (3) fritless, a microspray column having the above (1) to (3), a mass spectrometer having such a microspray column in an ionization source, and a mass spectrometric method capable of performing a nonoflow electrospray with the microspray column.

公开(公告)号：US20050129539A1

公开(公告)日：2005-06-16

申请号：US10509440

申请日：2003-07-24

Applicant: Kenichi Kudo ,  Yoshio Yamauchi

Inventor： Kenichi Kudo ,  Yoshio Yamauchi

IPC: G01N30/26 ,  G01N30/32 ,  G01N30/34 ,  G01N30/36 ,  G01N30/60 ,  F04B1/00 ,  F04B17/00

CPC classification number: G01N30/34 ,  G01N30/6095 ,  G01N2030/207 ,  G01N2030/326 ,  G01N2030/328

Abstract: It is an object of the present invention to improve the performance of gradient liquid transfer in nano/micro liquid chromatographs when solvents are mixed. In a gradient liquid transfer device and method for a nano/micro liquid chromatograph, an opening and closing unit (160) capable of opening and closing a solvent passage (150) for transferring a secondary solvent is provided in the vicinity of a solvent mixing section (152) in a liquid transfer system (130); a passage from a metering pump (146) of a liquid transfer section (142) to the opening and closing unit (160) is filled with the secondary solvent in advance; and an appropriate pressure is applied to the secondary solvent. Therefore, the entry of a primary solvent into the solvent passage for transferring the secondary solvent is suppressed. In another gradient liquid transfer device and method for a nano/micro liquid chromatograph, a liquid having a low compression rate is filled, in advance, in a part of a solvent passage subsequent to a metering pump of a liquid transfer section, and only the required amount of a secondary solvent is filled subsequent to the liquid. Therefore, the entry of the primary solvent into a solvent passage for transferring the secondary solvent is suppressed, and liquid-transfer performance is improved.

Abstract translation: 本发明的一个目的是提高溶剂混合时纳米/微液相色谱仪中梯度液体转移的性能。 在用于纳米/微量液相色谱的梯度液体转移装置和方法中，在溶剂混合部分附近设置能够打开和关闭用于转移二次溶剂的溶剂通道（150）的打开和关闭单元（160） （152）在液体输送系统（130）中; 从液体输送部分（142）的计量泵（146）到打开和关闭单元（160）的通道预先填充有二次溶剂; 并向第二溶剂施加适当的压力。 因此，抑制了用于转移二次溶剂的溶剂通道中的主要溶剂的进入。 在另一种用于纳米/微液相色谱仪的梯度液体转移装置和方法中，预先在液体转移部分的计量泵之后的一部分溶剂通道中填充低压缩比的液体， 在液体之后填充所需量的第二溶剂。 因此，抑制了主溶剂进入用于转移二次溶剂的溶剂通道，从而提高了液体传递性能。

公开(公告)号：US20050121608A1

公开(公告)日：2005-06-09

申请号：US10450608

申请日：2002-04-08

Applicant: Yoshio Yamauchi ,  Tohru Natsume ,  Toshiaki Isobe

Inventor： Yoshio Yamauchi ,  Tohru Natsume ,  Toshiaki Isobe

IPC: G01N27/62 ,  H01J49/04

CPC classification number: H01J49/0013 ,  H01J49/167

Abstract: A problem is to provide a microspray column capable of increasing the ionization efficiency, and a high sensitive mass spectrometer and a mass spectrometric method using such a microspray column. For solving this problem, we provide a column for introducing a sample in an ionization source of a mass spectrometer (ESI/MS) being designed to ionize a sample molecule with electrospray and introduce it into an analyzer, has structural features of: (1) 0.5 μm or less in an inner diameter of a tip opening of the column; (2) 0.5 μm or more and 5 μm or less in a particle size of a column filler; and (3) fritless, a microspray column having the above (1) to (3), a mass spectrometer having such a microspray column in an ionization source, and a mass spectrometric method capable of performing a nonoflow electrospray with the microspray column.

Abstract translation: 问题在于提供能够提高电离效率的微阵列，以及使用这种微球柱的高灵敏度质谱仪和质谱法。 为了解决这个问题，我们提供了一个用于在质谱仪（ESI / MS）的电离源中引入样品的色谱柱，该样品被设计为用电喷雾电离样品分子并将其引入分析仪，具有以下结构特征：（1） 柱的顶端开口的内径为0.5μm或更小; （2）柱填料的粒径为0.5μm以上且5μm以下; 和（3）无油玻璃，具有上述（1）〜（3）的微滴柱，在电离源中具有这样的微阵列的质谱仪，以及能够使用微喷射柱进行非离子电喷雾的质谱法。

公开(公告)号：US5180487A

公开(公告)日：1993-01-19

申请号：US715880

申请日：1991-06-17

Applicant: Muneo Saito ,  Yoshio Yamauchi

Inventor： Muneo Saito ,  Yoshio Yamauchi

IPC: B01D15/08 ,  G01N30/02 ,  G01N30/34

CPC classification number: B01D15/1814 ,  B01D15/40 ,  G01N30/02 ,  G01N30/34

Abstract: It is an object to provide a recycle chromatograph with supercritical fluid mobile phase for separating a sample into components by recycling a mobile phase which includes modifier solvent for the purpose of taking a desired component.It is structured in such a manner that a recycling pump (10), a column (14), a detector (16), and a three-way valve (18) are connected in a sequential order as described above to form a closed loop which acts as a recycle flow line. A back-pressure regulator (20) is connected to the remainder port (C) of this three-way valve (18), and a mobile phase supplying and pressurizing device (30, 38, 30A, 48) for supplying the mobile phase under a predetermined pressure is connected to a flow line between the three-way valve (18) and the recycling pump (10).By means of this mobile phase supplying and pressurizing device and the recycle closed loop, (the column pressure drop .DELTA.p)/(column plate number) can be reduced, whereupon the column effective plate number can be increased. Thus, a sufficient resolution rate for fractionating a component can be obtained after appropriate recycling times.Furthermore, by combining the mobile phase supplying device (30, 38 30A, 38A, 48) and the back-pressure regulator (20), the internal pressures in the column and the detector can be kept above the critical pressure even if unnecessary components are being removed or a necessary component is being fractionated. As a result of which fractionation can be performed.

Abstract translation: 本发明的目的是提供一种具有超临界流体流动相的再循环色谱仪，用于通过循环包含改性剂溶剂的流动相将样品分离成组分，以获得所需组分。 以如上所述的顺序连接回收泵（10），塔（14），检测器（16）和三通阀（18）的结构，形成闭环 其充当循环流动线。 一个背压调节器（20）连接到该三通阀（18）的剩余端口（C），以及一个流动相供应和加压装置（30,38,30a，48） 预定压力连接到三通阀（18）和回收泵（10）之间的流动管线。 通过该流动相供给和加压装置和再循环闭环，可以降低（柱压降DELTA p）/（塔板数），从而可以提高柱有效板数。 因此，在适当的回收时间之后，可以获得用于分馏成分的足够的分离率。 此外，通过组合流动相供给装置（30,38,30A，38A，48）和背压调节器（20），即使不需要的组分是，也可以将柱和检测器中的内部压力保持在临界压力以上 被去除或必要的组分被分馏。 结果可以进行分馏。

公开(公告)号：US20130327669A1

公开(公告)日：2013-12-12

申请号：US13990319

申请日：2011-11-29

Applicant: Masaaki Sueoka ,  Yoshio Yamauchi

Inventor： Masaaki Sueoka ,  Yoshio Yamauchi

IPC: B65D85/48

CPC classification number: B65D85/48 ,  B65D19/0036 ,  B65D57/00 ,  B65D71/0096 ,  B65D81/057 ,  B65D81/107 ,  B65D2519/00034 ,  B65D2519/00069 ,  B65D2519/00129 ,  B65D2519/00273 ,  B65D2519/00278 ,  B65D2519/00288 ,  B65D2519/00318 ,  B65D2519/00348 ,  B65D2519/00437 ,  B65D2519/00562 ,  B65D2519/0081 ,  B65D2519/00815 ,  B65D2571/00043 ,  B65D2571/00074 ,  B65D2571/00117 ,  H01L31/02

Abstract: A rectangular thin panel conveyance unit that prevents damage of a plurality of rectangular thin panels while conveying the plurality of rectangular thin panels that are stacked so as not to contact one another. A rectangular thin panel conveyance unit includes a plurality of sets of four corner modules, and vibration suppressing bodies for rectangular thin panels in a fixed shape. The corner module has a support surface to support each corner portion of the rectangular thin panel from a lower side. At each corner portion, stacking the corner modules in a columnar shape in the vertical direction stacks a plurality of rectangular thin panels in the vertical direction. At this time, the vibration suppressing body has the thickness that is equal to or less than the distance between the support surfaces of the corner modules adjacent to one another in the vertical direction. The vibration suppressing bodies are disposed on each of a top surface and an inferior surface of the rectangular thin panel such that the vibration suppressing body faces a planar portion of each rectangular thin panel to restrict amplitude of vibration of the rectangular thin panel in the vertical direction, which is caused by conveyance of the plurality of stacked rectangular thin panels, within a predetermined range. The vibration suppressing body has a contact portion for the planar portion of the rectangular thin panel.

Abstract translation: 矩形薄板输送单元，其防止多个矩形薄板在输送堆叠而不彼此接触的多个矩形薄板的同时损坏。 矩形薄板输送单元包括多组四个角模块，以及固定形状的矩形薄板振动抑制体。 角模块具有支撑表面，以从下侧支撑矩形薄板的每个拐角部分。 在每个角部分，垂直方向上以圆柱状堆叠角部模块在垂直方向堆叠多个矩形薄板。 此时，振动抑制体的厚度在垂直方向上彼此相邻的角部模块的支撑面之间的距离以下。 振动抑制体设置在矩形薄板的顶表面和下表面中，使得振动抑制体面向每个矩形薄板的平面部分，以限制矩形薄板在垂直方向上的振动幅度 ，这是由多个堆叠的矩形薄板的输送引起的。 振动抑制体具有用于矩形薄板的平面部分的接触部分。

公开(公告)号：US07402250B2

公开(公告)日：2008-07-22

申请号：US10509440

申请日：2003-07-24

Applicant: Kenichi Kudo ,  Yoshio Yamauchi

Inventor： Kenichi Kudo ,  Yoshio Yamauchi

IPC: B01D15/08

CPC classification number: G01N30/34 ,  G01N30/6095 ,  G01N2030/207 ,  G01N2030/326 ,  G01N2030/328

Abstract: It is an object of the present invention to improve the performance of gradient liquid transfer in nano/micro liquid chromatographs when solvents are mixed.In a gradient liquid transfer device and method for a nano/micro liquid chromatograph, an opening and closing unit (160) capable of opening and closing a solvent passage (150) for transferring a secondary solvent is provided in the vicinity of a solvent mixing section (152) in a liquid transfer system (130); a passage from a metering pump (146) of a liquid transfer section (142) to the opening and closing unit (160) is filled with the secondary solvent in advance; and an appropriate pressure is applied to the secondary solvent. Therefore, the entry of a primary solvent into the solvent passage for transferring the secondary solvent is suppressed. In another gradient liquid transfer device and method for a nano/micro liquid chromatograph, a liquid having a low compression rate is filled, in advance, in a part of a solvent passage subsequent to a metering pump of a liquid transfer section, and only the required amount of a secondary solvent is filled subsequent to the liquid. Therefore, the entry of the primary solvent into a solvent passage for transferring the secondary solvent is suppressed, and liquid-transfer performance is improved.

Abstract translation: 本发明的一个目的是提高溶剂混合时纳米/微液相色谱仪中梯度液体转移的性能。 在用于纳米/微量液相色谱的梯度液体转移装置和方法中，在溶剂混合部分附近设置能够打开和关闭用于转移二次溶剂的溶剂通道（150）的打开和关闭单元（160） （152）在液体输送系统（130）中; 从液体输送部分（142）的计量泵（146）到打开和关闭单元（160）的通道预先填充有二次溶剂; 并向第二溶剂施加适当的压力。 因此，抑制了用于转移二次溶剂的溶剂通道中的主要溶剂的进入。 在另一种用于纳米/微液相色谱仪的梯度液体转移装置和方法中，预先在液体转移部分的计量泵之后的一部分溶剂通道中填充低压缩比的液体， 在液体之后填充所需量的第二溶剂。 因此，抑制了主溶剂进入用于转移二次溶剂的溶剂通道，从而提高了液体传递性能。

公开(公告)号：US5013443A

公开(公告)日：1991-05-07

申请号：US465706

申请日：1990-01-16

Applicant: Sakae Higashidate ,  Yoshio Yamauchi ,  Muneo Saito

Inventor： Sakae Higashidate ,  Yoshio Yamauchi ,  Muneo Saito

IPC: B01D11/00 ,  C07B63/00 ,  C07C57/03 ,  C07C67/58 ,  C07C69/52 ,  C07C69/587 ,  C11B3/00 ,  C11B3/10 ,  G01N30/02 ,  G01N30/06 ,  G01N30/80

CPC classification number: C11B3/10 ,  C11B3/006 ,  G01N2030/062 ,  G01N30/02 ,  G01N30/80

Abstract: Extraction and separation method and apparatus using a supercritical fluid for extracting a specified component from a sample by a supercritical fluid and introducing the supercritical fluid containing the extracted component into a silica gel column coated with silver nitrate so as to separate the extracted component.By the combination of the extracting operation by a supercritical fluid under mild conditions and chromatography taking the advantage of the selectivity of the silica gel column coated with silver nitrate to olefins, the extract is obtained at a high concentration without producing denaturation.

公开(公告)号：US08880353B2

公开(公告)日：2014-11-04

申请号：US12988037

申请日：2009-04-17

Applicant: Hiroshi Nakayama ,  Misaki Akiyama ,  Toshiaki Isobe ,  Masato Taoka ,  Yoshio Yamauchi ,  Nobuhiro Takahashi ,  Hideaki Ishikawa

Inventor： Hiroshi Nakayama ,  Misaki Akiyama ,  Toshiaki Isobe ,  Masato Taoka ,  Yoshio Yamauchi ,  Nobuhiro Takahashi ,  Hideaki Ishikawa

IPC: G01N33/48 ,  C12Q1/68 ,  H01J49/00

CPC classification number: C12Q1/6872 ,  H01J49/00 ,  C12Q2521/307

Abstract: Disclosed are an apparatus for the identification of a ribonucleic acid, whereby not only the molecular weights of digestion products, from which nothing but the nucleic acid residue composition can be understood, but also nucleic acid residue sequence data is obtained from a product ion mass set and thus the identification reliability for the individual digestion products can be remarkably improved; a method for the identification of a ribonucleic acid; and a program and system for the identification of a ribonucleic acid. The method as described above comprises: searching for nucleic acid sequence database by using a fragment mass set indicating the molecular weights and inner structural data of individual oligonucleotides that are obtained by tandem mass spectrometry on digested oligonucleotides formed by chemically or enzymatically cleaving a ribonucleic acid; scoring candidate sequences; among candidate sequences showing the highest scores, referring those showing scores exceeding the threshold as identified sequences; and using these identified sequences in mapping on nucleic acid sequence database including genomic sequences.

Abstract translation: 公开了用于鉴定核糖核酸的装置，其不仅可以理解除了核酸残基组成之外的消化产物的分子量，还可以从核酸残基序列数据获得产物离子质量组 从而可以显着提高个体消化产品的鉴定可靠性; 用于鉴定核糖核酸的方法; 以及用于鉴定核糖核酸的程序和系统。 如上所述的方法包括：通过使用表示通过串联质谱法获得的各种寡核苷酸的分子量和内部结构数据的片段质谱组来搜索核酸序列数据库对通过化学或酶切割核糖核酸形成的消化的寡核苷酸; 得分候选序列; 在显示最高分数的候选序列中，将显示分数超过阈值的那些指定为鉴定序列; 并使用这些鉴定的序列在包括基因组序列的核酸序列数据库上进行测绘。

公开(公告)号：US20120312983A1

公开(公告)日：2012-12-13

申请号：US13590033

申请日：2012-08-20

Applicant: Toshiaki ISOBE ,  Yoshio YAMAUCHI

Inventor： Toshiaki ISOBE ,  Yoshio YAMAUCHI

IPC: H01J49/04 ,  H01J49/26

CPC classification number: H01J49/10 ,  G01N30/7266

Abstract: Nano-electrospray ionization techniques include the introduction of a separation solvent containing a sample to a column-integrated needle having a column filled with a resin for liquid chromatography. The separated sample components are sprayed from the tip of the column-integrated needle toward a sample introduction orifice of a mass spectrometer. An organic solvent is simultaneously introduced to a solvent-supplying needle. The organic solvent is supplied from the tip of the solvent-supplying needle to the tip of the column-integrated needle.

Abstract translation: 纳米电喷雾电离技术包括将含有样品的分离溶剂引入具有填充有用于液相色谱的树脂的柱的柱一体化针。 将分离的样品组分从柱集成针的尖端喷射到质谱仪的样品引入孔。 将有机溶剂同时引入溶剂供应针。 有机溶剂从溶剂供应针的尖端供应到列一体化针的末端。

公开(公告)号：US20110089319A1

公开(公告)日：2011-04-21

申请号：US12999434

申请日：2010-06-02

Applicant: Toshiaki Isobe ,  Yoshio Yamauchi

Inventor： Toshiaki Isobe ,  Yoshio Yamauchi

IPC: H01J49/04 ,  H01J49/26

CPC classification number: H01J49/10 ,  G01N30/7266

Abstract: Nano-electrospray ionization techniques include the introduction of a separation solvent containing a sample to a column-integrated needle having a column filled with a resin for liquid chromatography. The separated sample components are sprayed from the tip of the column-integrated needle toward a sample introduction orifice of a mass spectrometer. An organic solvent is simultaneously introduced to a solvent-supplying needle. The organic solvent is supplied from the tip of the solvent-supplying needle to the tip of the column-integrated needle.

Abstract translation: 纳米电喷雾电离技术包括将含有样品的分离溶剂引入具有填充有用于液相色谱的树脂的柱的柱一体化针。 将分离的样品组分从柱集成针的尖端喷射到质谱仪的样品引入孔。 将有机溶剂同时引入溶剂供应针。 有机溶剂从溶剂供应针的尖端供应到列一体化针的末端。