公开(公告)号：US20130184184A1

公开(公告)日：2013-07-18

申请号：US13302819

申请日：2011-11-22

Applicant: David A. Schwartz ,  Jimmy Williams ,  Xinfang Zhao ,  Chunfang Zhao ,  William B. Busa ,  Stephen J. Kron ,  Amy Catherine Flor

Inventor： David A. Schwartz ,  Jimmy Williams ,  Xinfang Zhao ,  Chunfang Zhao ,  William B. Busa ,  Stephen J. Kron ,  Amy Catherine Flor

IPC: C12Q1/68

CPC classification number: C12Q1/6804 ,  C07H21/00 ,  C12Q1/6834 ,  C12Q2565/113

Abstract: The present disclosure is directed to methods and/or uses of oligonucleotide conjugates having varied degrees of labeling for assays and detections and related systems and/or kits. Certain methods are directed to a method for detecting one or more biological targets of a sample in a detection assay, comprising: providing a molecular probe, comprising a binding moiety and an oligonucleotide sequence, to a sample comprising one or more biological targets; binding the one or more biological targets with the binding moiety; providing a detectable component to the sample, wherein the detectable component comprises a signal generating moiety conjugated to an oligonucleotide sequence complementary to the oligonucleotide sequence of the molecular probe; hydridizing the oligonucleotide sequence of the target-bound molecular probe to the detectable component; and detecting a signal generated from the hydridized detectable component. Various other embodiments, applications etc. are disclosed herein.

Abstract translation: 本公开涉及具有不同程度的标记用于测定和检测以及相关系统和/或试剂盒的寡核苷酸缀合物的方法和/或用途。 某些方法涉及用于在检测测定中检测样品的一种或多种生物学靶标的方法，其包括：向包含一种或多种生物靶标的样品提供包含结合部分和寡核苷酸序列的分子探针; 将一种或多种生物靶与结合部分结合; 向所述样品提供可检测组分，其中所述可检测组分包含与与所述分子探针的寡核苷酸序列互补的寡核苷酸序列缀合的信号产生部分; 将靶结合分子探针的寡核苷酸序列杂交到可检测组分; 以及检测由所述可混合的可检测组分产生的信号。 本文公开了各种其它实施例，应用等。

公开(公告)号：US20120258881A1

公开(公告)日：2012-10-11

申请号：US13302890

申请日：2011-11-22

Applicant: David A. Schwartz ,  Jimmy Williams ,  Xinfang Zhao ,  Chunfang Zhao ,  William B. Busa ,  Stephen J. Kron ,  Amy Catherine Flor

Inventor： David A. Schwartz ,  Jimmy Williams ,  Xinfang Zhao ,  Chunfang Zhao ,  William B. Busa ,  Stephen J. Kron ,  Amy Catherine Flor

IPC: C12Q1/68 ,  G01N21/76 ,  G01N21/00 ,  G01N21/59 ,  G01N21/47 ,  G01N21/64 ,  G01N21/25 ,  G01N21/66 ,  G01N21/65 ,  G01N24/00 ,  G01N27/72 ,  C40B30/04 ,  C12Q1/70 ,  B82Y15/00

CPC classification number: C12Q1/6804 ,  C07H21/00 ,  C12Q1/6834 ,  C12Q2565/113

Abstract: The present disclosure is directed to methods and/or uses of oligonucleotide conjugates for assays and microscopy/imaging detections and related systems and/or kits. Certain methods are directed to a method for detecting one or more biological targets of a sample in a detection assay, comprising: providing a molecular probe, comprising a binding moiety and an oligonucleotide sequence, to a sample comprising one or more biological targets; binding the one or more biological targets with the binding moiety; providing a detectable component to the sample, wherein the detectable component comprises a signal generating moiety conjugated to an oligonucleotide sequence complementary to the oligonucleotide sequence of the molecular probe; hydridizing the oligonucleotide sequence of the target-bound molecular probe to the detectable component; and detecting a signal generated from the hydridized detectable component. Various other embodiments, applications etc. are disclosed herein.

Abstract translation: 本公开涉及用于测定和显微镜/成像检测和相关系统和/或试剂盒的寡核苷酸缀合物的方法和/或用途。 某些方法涉及用于在检测测定中检测样品的一种或多种生物学靶标的方法，其包括：向包含一种或多种生物靶标的样品提供包含结合部分和寡核苷酸序列的分子探针; 将一种或多种生物靶与结合部分结合; 向所述样品提供可检测组分，其中所述可检测组分包含与与所述分子探针的寡核苷酸序列互补的寡核苷酸序列缀合的信号产生部分; 将靶结合分子探针的寡核苷酸序列杂交到可检测组分; 以及检测由所述可混合的可检测组分产生的信号。 本文公开了各种其它实施例，应用等。

公开(公告)号：US20130123121A1

公开(公告)日：2013-05-16

申请号：US13302801

申请日：2011-11-22

Applicant: David A. Schwartz ,  Jimmy Williams ,  Xinfang Zhao ,  Chunfang Zhao ,  William B. Busa ,  Stephen J. Kron ,  Amy Catherine Flor

Inventor： David A. Schwartz ,  Jimmy Williams ,  Xinfang Zhao ,  Chunfang Zhao ,  William B. Busa ,  Stephen J. Kron ,  Amy Catherine Flor

IPC: C12Q1/68

CPC classification number: C12Q1/6804 ,  C07H21/00 ,  C12Q1/6834 ,  C12Q2565/113

Abstract: The present disclosure is directed to methods and/or uses of oligonucleotide-bead conjugates for assays and detections and related systems and/or kits. Certain methods are directed to a method for detecting one or more biological targets of a sample in a detection assay, comprising: providing a molecular probe, comprising a binding moiety and an oligonucleotide sequence, to a sample comprising one or more biological targets; binding the one or more biological targets with the binding moiety; providing a detectable component to the sample, wherein the detectable component comprises a signal generating moiety conjugated to an oligonucleotide sequence complementary to the oligonucleotide sequence of the molecular probe; hydridizing the oligonucleotide sequence of the target-bound molecular probe to the detectable component; and detecting a signal generated from the hydridized detectable component. Various other embodiments, applications etc. are disclosed herein.

Abstract translation: 本公开涉及用于测定和检测以及相关系统和/或试剂盒的寡核苷酸 - 珠偶联物的方法和/或用途。 某些方法涉及用于在检测测定中检测样品的一种或多种生物学靶标的方法，其包括：向包含一种或多种生物靶标的样品提供包含结合部分和寡核苷酸序列的分子探针; 将一种或多种生物靶与结合部分结合; 向所述样品提供可检测组分，其中所述可检测组分包含与与所述分子探针的寡核苷酸序列互补的寡核苷酸序列缀合的信号产生部分; 将靶结合分子探针的寡核苷酸序列杂交到可检测组分; 以及检测由所述可混合的可检测组分产生的信号。 本文公开了各种其它实施例，应用等。

公开(公告)号：US20120258870A1

公开(公告)日：2012-10-11

申请号：US13302910

申请日：2011-11-22

Applicant: David A. Schwartz ,  Jimmy Williams ,  Xinfang Zhao ,  Chunfang Zhao ,  William B. Busa ,  Stephen J. Kron ,  Amy Catherine Flor

Inventor： David A. Schwartz ,  Jimmy Williams ,  Xinfang Zhao ,  Chunfang Zhao ,  William B. Busa ,  Stephen J. Kron ,  Amy Catherine Flor

IPC: C40B20/04

CPC classification number: C12Q1/6804 ,  C07H21/00 ,  C12Q1/6834 ,  C12Q2565/113

Abstract: The present disclosure is directed to methods systems, and/or uses of oligonucleotide conjugates to develop panels for use in assays and detections and related systems and/or kits. Certain methods are directed to a method for detecting one or more biological targets of a sample in a detection assay, comprising: providing a molecular probe, comprising a binding moiety and an oligonucleotide sequence, to a sample comprising one or more biological targets; binding the one or more biological targets with the binding moiety; providing a detectable component to the sample, wherein the detectable component comprises a signal generating moiety conjugated to an oligonucleotide sequence complementary to the oligonucleotide sequence of the molecular probe; hydridizing the oligonucleotide sequence of the target-bound molecular probe to the detectable component; and detecting a signal generated from the hydridized detectable component. Various other embodiments, applications etc. are disclosed herein.

Abstract translation: 本公开涉及寡核苷酸缀合物开发面板以用于测定和检测以及相关系统和/或试剂盒的方法系统和/或用途。 某些方法涉及用于在检测测定中检测样品的一种或多种生物学靶标的方法，其包括：向包含一种或多种生物靶标的样品提供包含结合部分和寡核苷酸序列的分子探针; 将一种或多种生物靶与结合部分结合; 向所述样品提供可检测组分，其中所述可检测组分包含与与所述分子探针的寡核苷酸序列互补的寡核苷酸序列缀合的信号产生部分; 将靶结合分子探针的寡核苷酸序列杂交到可检测组分; 以及检测由所述可混合的可检测组分产生的信号。 本文公开了各种其它实施例，应用等。

公开(公告)号：US20130035259A1

公开(公告)日：2013-02-07

申请号：US13302851

申请日：2011-11-22

Applicant: David A. Schwartz ,  Jimmy Williams ,  Xinfang Zhao ,  Chunfang Zhao ,  William B. Busa ,  Stephen J. Kron ,  Amy Catherine Flor

Inventor： David A. Schwartz ,  Jimmy Williams ,  Xinfang Zhao ,  Chunfang Zhao ,  William B. Busa ,  Stephen J. Kron ,  Amy Catherine Flor

IPC: C40B40/06 ,  C40B50/00

CPC classification number: C12Q1/6804 ,  C07H21/00 ,  C12Q1/6834 ,  C12Q2565/113

Abstract: The present disclosure is directed to methods and/or systems producing and providing uses sets of oligonucleotide conjugates for assays and detections and related systems and/or kits. Certain methods are directed to a method for detecting one or more biological targets of a sample in a detection assay, comprising: providing a molecular probe, comprising a binding moiety and an oligonucleotide sequence, to a sample comprising one or more biological targets; binding the one or more biological targets with the binding moiety; providing a detectable component to the sample, wherein the detectable component comprises a signal generating moiety conjugated to an oligonucleotide sequence complementary to the oligonucleotide sequence of the molecular probe; hydridizing the oligonucleotide sequence of the target-bound molecular probe to the detectable component; and detecting a signal generated from the hydridized detectable component. Various other embodiments, applications etc. are disclosed herein.

Abstract translation: 本公开涉及产生和提供用于测定和检测以及相关系统和/或试剂盒的寡核苷酸缀合物组的方法和/或系统。 某些方法涉及用于在检测测定中检测样品的一种或多种生物学靶标的方法，其包括：向包含一种或多种生物靶标的样品提供包含结合部分和寡核苷酸序列的分子探针; 将一种或多种生物靶与结合部分结合; 向所述样品提供可检测组分，其中所述可检测组分包含与与所述分子探针的寡核苷酸序列互补的寡核苷酸序列缀合的信号产生部分; 将靶结合分子探针的寡核苷酸序列杂交到可检测组分; 以及检测由所述可混合的可检测组分产生的信号。 本文公开了各种其它实施例，应用等。

公开(公告)号：US20120258880A1

公开(公告)日：2012-10-11

申请号：US13302877

申请日：2011-11-22

Applicant: David A. Schwartz ,  Jimmy Williams ,  Xinfang Zhao ,  Chunfang Zhao ,  William B. Busa ,  Stephen J. Kron ,  Amy Catherine Flor

Inventor： David A. Schwartz ,  Jimmy Williams ,  Xinfang Zhao ,  Chunfang Zhao ,  William B. Busa ,  Stephen J. Kron ,  Amy Catherine Flor

IPC: G01N33/53 ,  C40B30/04 ,  G01N21/47 ,  G01N21/59 ,  G01N21/64 ,  G01N27/26 ,  G01N24/00 ,  G01N21/65 ,  G01N21/66 ,  G01N21/25 ,  G01N27/72 ,  C12Q1/68 ,  G01N21/76 ,  B82Y15/00

CPC classification number: C12Q1/6804 ,  C07H21/00 ,  C12Q1/6834 ,  C12Q2565/113

Abstract: The present disclosure is directed to methods and/or uses of oligonucleotide conjugates for assays and flow cytometry detections and related systems and/or kits. Certain methods are directed to a method for detecting one or more biological targets of a sample in a detection assay, comprising: providing a molecular probe, comprising a binding moiety and an oligonucleotide sequence, to a sample comprising one or more biological targets; binding the one or more biological targets with the binding moiety; providing a detectable component to the sample, wherein the detectable component comprises a signal generating moiety conjugated to an oligonucleotide sequence complementary to the oligonucleotide sequence of the molecular probe; hydridizing the oligonucleotide sequence of the target-bound molecular probe to the detectable component; and detecting a signal generated from the hydridized detectable component. Various other embodiments, applications etc. are disclosed herein.

Abstract translation: 本公开涉及寡核苷酸缀合物用于测定和流式细胞术检测及相关系统和/或试剂盒的方法和/或用途。 某些方法涉及用于在检测测定中检测样品的一种或多种生物学靶标的方法，其包括：向包含一种或多种生物靶标的样品提供包含结合部分和寡核苷酸序列的分子探针; 将一种或多种生物靶与结合部分结合; 向所述样品提供可检测组分，其中所述可检测组分包含与与所述分子探针的寡核苷酸序列互补的寡核苷酸序列缀合的信号产生部分; 将靶结合分子探针的寡核苷酸序列杂交到可检测组分; 以及检测由所述可混合的可检测组分产生的信号。 本文公开了各种其它实施例，应用等。

公开(公告)号：US07356416B2

公开(公告)日：2008-04-08

申请号：US09768686

申请日：2001-01-24

Applicant: William B. Busa

Inventor： William B. Busa

IPC: G01N33/48 ,  G06G7/48 ,  G06F7/00

CPC classification number: G06F17/277 ,  G06F17/2715 ,  G06F19/12 ,  G06F19/18 ,  G06F19/24 ,  G06F19/28 ,  Y02A90/26 ,  Y10S707/99931

Abstract: Methods and system for automated inference of physico-chemical interaction knowledge from databases of term co-occurrence data. The co-occurrence data includes co-occurrences between chemical or biological molecules or co-occurrences between chemical or biological molecules and biological processes. Likelihood statistics are determined and applied to decide if co-occurrence data reflecting physico-chemical interactions is non-trivial. A next node or an unknown target representing chemical or biological molecules in a biological pathway is selected based on co-occurrence values. The method and system may be used to further facilitate a user's understanding of biological functions, such as cell functions, to design experiments more intelligently and to analyze experimental results more thoroughly. Specifically, the present invention may help drug discovery scientists select better targets for pharmaceutical intervention in the hope of curing diseases. The method and system may also help facilitate the abstraction of knowledge from information for biological experimental data and provide new bioinformatic techniques.

Abstract translation: 用于自动推断术语同时发生数据数据库的物理 - 化学相互作用知识的方法和系统。 共生数据包括化学或生物分子之间的共同出现或化学或生物分子与生物过程之间的共同出现。 确定并应用似然统计量来确定反映物理 - 化学相互作用的同现数据是否不平凡。 基于同现值选择表示生物学途径中的化学或生物分子的下一个节点或未知目标。 该方法和系统可以用于进一步促进用户对诸如细胞功能等生物学功能的理解，更智能地设计实验并且更彻底地分析实验结果。 具体来说，本发明可以帮助药物发现科学家选择更好的药物干预目标，以希望治愈疾病。 该方法和系统还可以帮助促进从生物实验数据的信息中抽取知识并提供新的生物信息学技术。