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1.发明授权Method, vectors and transformants for high expression of heterologous polypeptides in yeast 失效用于在酵母中高表达异源多肽的方法，载体和转化体
公开(公告)号：US4745057A
公开(公告)日：1988-05-17
申请号：US640422
申请日：1984-08-13
摘要： Disclosed are a novel method for inducing the high expression of a nucleotide sequence which is under the transcriptional and translational control of the yeast YG100 gene and the novel vectors, transformants and selectable DNA for the practice thereof.
摘要翻译： 公开了一种用于诱导在酵母YG100基因的转录和翻译控制下的核苷酸序列的高表达的新方法，以及用于实施它们的新载体，转化体和可选择的DNA。
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2.发明授权Recombinant DNA expression vectors and DNA compounds that encode deacetoxycephalosporin C synthetase and deacetylcephalosporin C synthetase 失效重组DNA表达载体和编码脱乙酰头孢菌素C合成酶和脱乙酰头孢菌素C合成酶的DNA化合物公开(公告)号：US06180361B2
公开(公告)日：2001-01-30
申请号：US07283429
申请日：1988-12-12
IPC分类号： C12N1513
CPC分类号： C12N9/0071 , C12N15/52 , C12N15/70 , C12N15/80
摘要： The present invention provides DNA compounds that encode the expandase/hydroxylase enzyme of Cephalosporium acremonium. The compounds can be used to construct recombinant DNA expression vectors for a variety of host cells, including E. coli, Penicillium, and Cephalosporium.
摘要翻译： 本发明提供编码头孢霉头孢菌的扩环酶/羟化酶的DNA化合物。 该化合物可用于构建多种宿主细胞的重组DNA表达载体，包括大肠杆菌，青霉菌和头孢菌素。
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3.发明授权Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from penicillium chrysogenum 失效重组DNA表达载体和编码来自产黄青霉的异青霉素N合成酶的DNA化合物
公开(公告)号：US4892819A
公开(公告)日：1990-01-09
申请号：US801523
申请日：1985-11-25
IPC分类号： C12N15/09 , C12N1/14 , C12N1/15 , C12N1/20 , C12N1/21 , C12N9/00 , C12N15/00 , C12N15/52 , C12N15/69 , C12N15/70 , C12N15/80 , C12N15/90 , C12P37/00 , C12R1/19 , C12R1/75 , C12R1/82
摘要： The present invention comprises novel DNA compounds that encode isopenicillin N synthetase. The invention also comprises methods, transformants, and polypeptides related to the novel DNA compounds. The novel isopenicillin N synthetase-encoding DNA, together with its associated transcription and translation activating sequence, was isolated from Penicillium chrysogenum. The isopenicillin N synthetase-encoding DNA can be used to construct novel E. coli expression vectors that drive expression of isopenicillin N synthetase in E. coli. The intact P. chrysogenum isopenicillin N synthetase-encoding DNA and associated transcription and translation activating sequence can also be used to construct expression vectors that drive expression of the isopenicillin N synthetase in P. chrysogenum and Cephalosporium acremonium. The transcription and translation activating sequence can be fused to a hygromycin phosphotransferase-encoding DNA segment and placed onto expression vectors that function in P. chrysogenum and C. acremonium. The transcription termination and mRNA polyadenylation signals of the P. chrysogenum isopenicillin N synthetase can be used to increase ultimate expression of a product encoded on a recombinant DNA vector.
摘要翻译： 本发明包括编码异青霉素N合成酶的新型DNA化合物。 本发明还包括与新型DNA化合物相关的方法，转化体和多肽。 编码新颖的异青霉素N合成酶的DNA，连同其相关的转录和翻译活化序列，从产黄青霉中分离出来。 编码异青霉素N合成酶的DNA可用于构建驱动大肠杆菌中异青霉素N合成酶表达的新型大肠杆菌表达载体。 完整的产黄青霉素异青霉素N合成酶编码DNA和相关的转录和翻译活化序列也可用于构建表达产物，其驱动产黄青霉和顶头孢霉的异青霉素N合成酶的表达。 转录和翻译激活序列可以与编码潮霉素磷酸转移酶的DNA片段融合，并置于表达载体中，该表达载体在产黄青霉和顶头孢霉中起作用。 产黄青霉素异青霉素N合成酶的转录终止和mRNA聚腺苷酸化信号可用于增加编码在重组DNA载体上的产物的最终表达。
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公开(公告)号：US4559302A
公开(公告)日：1985-12-17
申请号：US438070
申请日：1982-11-01
申请人： Thomas D. Ingolia
发明人： Thomas D. Ingolia
CPC分类号： C12N15/70 , Y10S435/849 , Y10S435/886
摘要： The present invention relates to a novel transcriptional and translational activating sequence. The novel activating sequence can be either chemically synthesized or isolated on a 0.17 kb PstI-SacI restriction fragment from plasmid pKC203, a plasmid of E. coli JR225 (ATCC 31912). The activating sequence directs expression of the aminoglycoside acetyltransferase aac(3)IV and hygromycin phosphotransferase aph(4) genes present on plasmid pKC203. A series of expression vectors have been constructed in which the activating sequence directs the expression of beta-galactosidase or hygromycin phosphotransferase. These vectors can be readily modified and have been designed to facilitate the subsequent cloning and expression of any gene of research or commercial interest. The expression and cloning vectors have been transformed into E. coli and other host cells in which the activating sequence functions.
摘要翻译： 本发明涉及一种新的转录和翻译活化序列。 新的活化序列可以在质粒pKC203（大肠杆菌JR225（ATCC 31912）的质粒）的0.17kb PstI-SacI限制性片段上化学合成或分离。 活化序列指示存在于质粒pKC203上的氨基糖苷酰乙酰转移酶aac（3）IV和潮霉素磷酸转移酶aph（4）基因的表达。 已经构建了一系列表达载体，其中活化序列指导β-半乳糖苷酶或潮霉素磷酸转移酶的表达。 这些载体可以容易地被修饰并被设计成有助于随后克隆和表达任何研究或商业利益的基因。 表达和克隆载体已经转化到大肠杆菌和其中启动序列起作用的其它宿主细胞。
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公开(公告)号：US5196524A
公开(公告)日：1993-03-23
申请号：US294170
申请日：1989-01-06
CPC分类号： C12N9/0071 , C12N15/80 , C12N15/8209 , C12N15/85 , C12Y114/14003 , C07K2319/00 , C12N2830/002 , C12N2830/42 , C12N2830/70 , C12N2830/702 , C12N2840/20
摘要： Novel fusion reporter genes, fusion reporter proteins, and an improved reporter system for measuring the relative activity of a promoter sequence. A luxAB fusion gene of the present invention is particularly useful as a reporter gene and is derived from the fusion of a luxA gene and a luxB gene from Vibrio harveyi. The gene products of the luxA and luxB genes are the .alpha.- and .beta.-subunits, respectively, of a bacterial luciferase. A fusion protein encoded by a luxAB fusion gene is a single active protein and is particularly useful as a reporter protein having luciferase activity. An advantage of such a reporter system to assay gene expression in many cells which contain FMNH.sub.2, such as bacterial and yeast cells, is that an immediate and quantitative assessment of gene expression may be made from real-time light measurements using intact cells.
摘要翻译： 新型融合报告基因，融合报道蛋白，以及用于测量启动子序列相对活性的改进的报道系统。 本发明的luxAB融合基因特别可用作报告基因，并且来源于来自哈氏弧菌的luxA基因和luxB基因的融合。 luxA和luxB基因的基因产物分别是细菌荧光素酶的α和β亚基。 由luxAB融合基因编码的融合蛋白是单一活性蛋白，并且特别可用作具有荧光素酶活性的报道蛋白。 这种报告系统在许多含有FMNH2的细胞（如细菌和酵母细胞）中测定基因表达的优点是可以使用完整的细胞从实时光测量中进行基因表达的即时和定量评估。
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6.发明授权Recombinant DNA expression vectors and DNA compounds which encode isopenicillin N synthetase 失效编码异青霉素N合成酶的重组DNA表达载体和DNA化合物
公开(公告)号：US4885251A
公开(公告)日：1989-12-05
申请号：US895008
申请日：1986-08-08
摘要： The present invention comprises novel DNA compounds that encode isopenicillin N synthetase and also comprises related methods, transformants, and polypeptides. The novel isopenicillin N synthetase-encoding DNA, together with its associated transcriptional and translational activating sequence, was isolated from Cephalosporium acremonium and cloned into an E. coli cloning vector. The isopenicillin N synthetase-encoding DNA has been used to construct novel E. coli expression vectors that drive expression of a stable, active, and novel isopenicillin N synthetase in E. coli. The intact C. acremonium isopenicillin N synthetase-encoding DNA and associated transcriptional and translational activating sequence have also been used to construct C. acremonium expression vectors that drive expression of the isopenicillin N synthetase in C. acremonium. The C. acremonium transcriptional and translational activating sequence has further been fused to a hygromycin phosphotransferase-encoding DNA segment and placed onto C. acremonium expression vectors. Useful derivatives of the novel compounds and vectors are also described.
摘要翻译： 本发明包括编码异青霉素N合成酶的新型DNA化合物，还包括相关方法，转化体和多肽。 编码新的异青霉素N合成酶DNA，连同其相关的转录和翻译活化序列，从头孢霉头孢菌分离并克隆到大肠杆菌克隆载体中。 编码异青霉素N合成酶的DNA已经用于构建新的大肠杆菌表达载体，其在大肠杆菌中驱动稳定的，活性的和新的异青霉素N合成酶的表达。 编码完整的顶头孢霉素异青霉素N合成酶的DNA和相关的转录和翻译活化序列也被用于构建顶头孢霉表达载体，其驱动顶头孢霉中异青霉素N合成酶的表达。 顶头孢霉转录和翻译激活序列进一步融合到编码潮霉素磷酸转移酶的DNA片段并置于顶头孢霉表达载体上。 还描述了新化合物和载体的有用衍生物。
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公开(公告)号：US20110311682A1
公开(公告)日：2011-12-22
申请号：US13203222
申请日：2010-02-24
CPC分类号： A23J3/22 , A23J3/04 , A23J3/08 , A23J3/16 , A23L19/09 , A23L21/12 , A23L29/281 , A23L29/35 , A23V2002/00 , A61K31/716
摘要： Nutritious protein-containing foodstuffs can be prepared in the form of gels, including protein gel and fruit purees that contain all essential amino acids. A protein el for use as a foodstuff is prepared by heating an aqueous protein solution to between 160 and 185 degrees Fahrenheit at an acidic pH between 2.85 and 3.5, the protein solution having a viscosity greater than 100 centipoise and containing a protein at a concentration of 10-20% w/w, the protein being at least one of a whey protein isolate and a liquid soy protein isolate, the protein gel having a viscosity of between 10,000 and 1,000,000 centipoise.
摘要翻译： 含营养蛋白质的食品可以以凝胶的形式制备，包括含有所有必需氨基酸的蛋白质凝胶和水果泥。 用于食品的蛋白质el通过在2.85至3.5之间的酸性pH下将蛋白质水溶液加热至160至185华氏度而制备，所述蛋白质溶液的粘度大于100厘泊，并且含有浓度为 10-20％w / w，所述蛋白质是乳清蛋白分离物和液体大豆蛋白分离物中的至少一种，所述蛋白质凝胶的粘度为10,000至1,000,000厘泊。
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8.发明授权Novel DNA for expression of delta-aminolevulinic acid synthetase and related method 失效用于表达δ-氨基乙酰丙酸合成酶的新型DNA及相关方法
公开(公告)号：US4902620A
公开(公告)日：1990-02-20
申请号：US725876
申请日：1985-04-25
申请人： Martin Bard , Thomas D. Ingolia
发明人： Martin Bard , Thomas D. Ingolia
CPC分类号： C12N9/1029 , C12N15/64 , C12N15/68 , C12N15/81 , C12P13/005 , C12R1/865 , Y10S435/94
摘要： The present invention is a novel method for maintaining and selecting recombinant DNA-containing host cells wherein the DNA encoding a selectable phenotype and the DNA encoding a useful polypeptide are the same. The aforementioned DNA is useful for expressing .delta.-aminolevulinic acid synthetase (ALAS) for the ultimate expression of .delta.-aminolevulinic acid (ALA) in yeast and related organisms. The invention further comprises plasmids pIT300, pIT301, pIT302, pIT304, pIT305, pIT306 and related Saccharomyces ALA deficient transformants. ALA is a five carbon amino acid that is useful as a light dependent herbicide.
摘要翻译： 本发明是维持和选择重组含DNA宿主细胞的新方法，其中编码可选表型的DNA和编码有用多肽的DNA相同。 上述DNA可用于表达δ-氨基乙酰丙酸合成酶（ALAS），用于在酵母和相关生物体中最终表达δ-氨基乙酰丙酸（ALA）。 本发明进一步包括质粒pIT300，pIT301，pIT302，pIT304，pIT305，pIT306和相关的阿拉伯糖酵母缺失型转化体。 ALA是一种五碳氨基酸，可用作光依赖性除草剂。
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9.发明授权Recombinant DNA expression vectors and DNA compounds that encode deacetoxycephalosporin C synthetase 失效重组DNA表达载体和编码脱乙酰壳多糖C合成酶的DNA化合物
公开(公告)号：US5070020A
公开(公告)日：1991-12-03
申请号：US192273
申请日：1988-05-09
CPC分类号： C12N9/0071
摘要： The present invention provides DNA compounds that encode deacetoxycephalosporin C synthetase (DAOCS) activity. The compounds can be used to construct recombinant DNA expression vectors for a wide variety of host cells, including E. coli, Penicillium, Streptomyces, Aspergillus, and Cephalosporium.
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公开(公告)号：US4960704A
公开(公告)日：1990-10-02
申请号：US205011
申请日：1988-05-31
CPC分类号： C07K14/62 , C07K14/57581 , C12N15/62 , C12N15/81 , C12N15/8209 , C12N9/12 , C07K2319/00 , C07K2319/02
摘要： A modified hygromycin B resistance-conferring gene either alone or in translational reading phase with a gene or portion of a gene is disclosed. The invention further comprises recombinant DNA cloning vectors and transformants of the aforementioned DNA.
摘要翻译： 公开了改良的潮霉素B抗性基因，其单独或翻译阅读阶段与基因或基因的一部分。 本发明还包括重组DNA克隆载体和上述DNA的转化体。
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