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公开(公告)号：US4918166A
公开(公告)日：1990-04-17
申请号：US112083
申请日：1987-10-26
CPC分类号： C12N15/90 , C07K14/005 , C12N15/62 , C12N15/81 , C07K2319/00 , C07K2319/40 , C12N2740/16122 , Y10S530/806 , Y10S530/812 , Y10S530/824 , Y10S530/826
摘要： Fusion proteins comprise a77 first amino acid sequence and a second amino acid sequence. The first amino acid sequence is derived from a retrotransposon or an RNA retrovirus and confers on the fusion protein the ability to assemble into particles; an example is the product of the YTA gene of the yeast retrotransposon Ty. The second amino acid sequence is an HIV antigen. So particles formed of the fusion proteins may be useful in vaccines or in diagnostic or purification applications.
摘要翻译： 融合蛋白包含77个第一氨基酸序列和第二个氨基酸序列。 第一个氨基酸序列衍生自反转录转座子或RNA逆转录病毒，并赋予融合蛋白质组装成粒子的能力; 一个例子是酵母反转录转座子Ty的YTA基因的产物。 第二个氨基酸序列是HIV抗原。 因此，由融合蛋白形成的颗粒可用于疫苗或诊断或纯化应用。
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公开(公告)号：US4615974A
公开(公告)日：1986-10-07
申请号：US408826
申请日：1982-08-17
IPC分类号： C12N15/09 , C07H21/04 , C07K14/56 , C12N1/16 , C12N15/00 , C12N15/81 , C12P21/00 , C12R1/865 , C12N1/18 , C12P21/02
CPC分类号： C07K14/56 , C12N15/81 , Y10S930/142
摘要： There are described a number of plasmid vectors suitable for the expression of genetic material, at various levels in yeasts. The plasmids each comprise a yeast selective marker, a yeast replication origin and a yeast promoter positioned relative to a unique restriction site in such a way that expression may be obtained of a polypeptide coding sequence inserted at the restriction site. The promoters used are derived from the 5' region of a gene coding for a yeast glycolytic enzyme e.g. phosphoglycerate kinase (PGK), or from the 5' region of the yeast TRP1 gene. In one Example a plasmid contains a promoter derived from both the 3' and 5' regions of the PGK gene. The replication systems used involve the yeast 2.mu. replication origin or an autonomous replicating sequence (ARS) stabilized with an ARS stabilizing sequence (ASS). The replication systems allow for a choice of high or low copy number per cell. The promoter sequences allow for a choice of high or low expression level. A kit including vectors having a combination of these alternative features is described. Yeast expression vectors including a gene for coding for human interferon-.alpha. are described.
摘要翻译： 描述了许多适于在酵母中各种水平表达遗传物质的质粒载体。 质粒各自包含酵母选择性标记，酵母复制起点和相对于独特的限制性位点定位的酵母启动子，使得可以获得插入限制性位点的多肽编码序列的表达。 所用的启动子来源于编码酵母糖酵解酶的基因的5'区域， 磷酸甘油酸激酶（PGK），或来自酵母TRP1基因的5'区。 在一个实施例中，质粒含有源自PGK基因的3'和5'区两者的启动子。 所使用的复制系统涉及用ARS稳定序列（ASS）稳定的酵母2微复制起点或自主复制序列（ARS）。 复制系统允许每个单元格选择高或低拷贝数。 启动子序列允许选择高或低表达水平。 描述了包括具有这些替代特征的组合的载体的试剂盒。 描述了包含用于编码人类干扰素-α的基因的酵母表达载体。
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公开(公告)号：US09738907B2
公开(公告)日：2017-08-22
申请号：US10841603
申请日：2004-05-07
申请人： Philippa Radcliffe , James E. Miskin , Fraser J. Wilkes , Kyriacos A. Mitrophanous , Susan M. Kingsman
发明人： Philippa Radcliffe , James E. Miskin , Fraser J. Wilkes , Kyriacos A. Mitrophanous , Susan M. Kingsman
IPC分类号： C12N15/867 , C12N15/11 , C12N7/00 , A61K48/00 , C12N15/86
CPC分类号： C12N15/86 , A61K48/00 , C12N2740/15043 , C12N2830/003 , C12N2830/008 , C12N2830/50 , C12N2830/85 , C12N2840/20 , C12N2840/203 , C12N2840/44
摘要： Provided is a multicistronic retroviral vector genome having a first nucleic acid sequence upstream of at least one internal regulatory element, such that the level of genomic RNA available for packaging in the absence of rev, or a functional equivalent thereof, is increased.
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公开(公告)号：US5463024A
公开(公告)日：1995-10-31
申请号：US115397
申请日：1993-09-01
申请人： Alan J. Kingsman , Susan M. Kingsman , Sally E. Adams , Elizabeth J. C. Mellor , Michael H. Malim
发明人： Alan J. Kingsman , Susan M. Kingsman , Sally E. Adams , Elizabeth J. C. Mellor , Michael H. Malim
IPC分类号： C07K14/11 , C07K14/16 , C07K14/555 , C12N15/33 , C12N15/62 , C12N15/81 , C12N15/90 , C07K1/00 , C12N15/58
CPC分类号： C07K14/005 , C07K14/555 , C12N15/62 , C12N15/81 , C12N15/90 , C07K2319/00 , C07K2319/40 , C07K2319/75 , C12N2740/16122 , C12N2740/16222 , C12N2760/16022 , C12N2760/16122
摘要： Fusion proteins comprise a first amino acid sequence and a second amino acid sequence. The first amino acid sequence is derived from a retrotransposon or an RNA retrovirus and confers on the fusion protein the ability to assemble into particles; an example is the product of the TYA gene of the yeast retrotransposon Ty. The second amino acid sequence is biologically active; for example it may be antigenic. So particles formed of the fusion proteins may be useful in vaccines or in diagnostic or purification applications.
摘要翻译： 融合蛋白质包含第一个氨基酸序列和第二个氨基酸序列。 第一个氨基酸序列衍生自反转录转座子或RNA逆转录病毒，并赋予融合蛋白质组装成粒子的能力; 一个例子是酵母反转录转座子Ty的TYA基因的产物。 第二个氨基酸序列具有生物活性; 例如它可能是抗原性的。 因此，由融合蛋白形成的颗粒可用于疫苗或诊断或纯化应用。
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公开(公告)号：US5008373A
公开(公告)日：1991-04-16
申请号：US112082
申请日：1987-10-26
申请人： Alan J. Kingsman , Susan M. Kingsman , Sally E. Adams , Elizabeth J. C. Mellor , Michael H. Malim
发明人： Alan J. Kingsman , Susan M. Kingsman , Sally E. Adams , Elizabeth J. C. Mellor , Michael H. Malim
IPC分类号： C07K14/11 , C07K14/16 , C07K14/555 , C12N15/33 , C12N15/62 , C12N15/81 , C12N15/90 , C07K3/00 , C12N15/11
CPC分类号： C07K14/005 , C07K14/555 , C12N15/62 , C12N15/81 , C12N15/90 , C07K2319/00 , C07K2319/40 , C07K2319/75 , C12N2740/16122 , C12N2740/16222 , C12N2760/16022 , C12N2760/16122
摘要： Fusion proteins comprise a first amino acid sequence and a second amino acid sequence. The first amino acid sequence is derived from a retrotransposon or an RNA retrovirus and confers on the fusion protein the ability of assemble into particles; an example is the product of the TYA gene of the yeast retrotransposon Ty. The second amino acid sequence is biologically active; for example, it may be antigenic. So particles formed of the fusion proteins may be useful in vaccines or in diagnostic or purification applications
摘要翻译： 融合蛋白质包含第一个氨基酸序列和第二个氨基酸序列。 第一个氨基酸序列来源于反转录转座子或RNA逆转录病毒，并赋予融合蛋白质组装成粒子的能力; 一个例子是酵母反转录转座子Ty的TYA基因的产物。 第二个氨基酸序列具有生物活性; 例如，它可以是抗原性的。 因此，由融合蛋白形成的颗粒可用于疫苗或诊断或纯化应用
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公开(公告)号：US4898823A
公开(公告)日：1990-02-06
申请号：US165388
申请日：1988-02-29
CPC分类号： C12N15/81
摘要： An upstream activator sequence derived from the yeast PGK gene. The upstream activator sequence is contained in the 5' region of the yeast PGK gene between nucleotides -324 and -455. The upstream activator sequence has synthetic linkers attached to either end to facilitate attachment to a vector. Vectors including the upstream activator sequence and a heterologous promoter sequence are described. The upstream activator sequence is used in a method for increasing the expression level of a yeast expression vector.
摘要翻译： PCT No.PCT / GB84 / 00189 Sec。 371日期1985年1月30日 102（e）日期1985年1月30日PCT申请日1984年5月31日PCT公布。 公开号WO84 / 04757 日期1984年12月6日。来自酵母PGK基因的上游激活物序列。 上游激活物序列包含在酵母PGK基因的5'区域之间，核苷酸-324和-455之间。 上游活化剂序列具有连接到任一端的合成接头以便于与载体的连接。 描述了包含上游活化剂序列和异源启动子序列的载体。 上游活化剂序列用于增加酵母表达载体的表达水平的方法中。
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公开(公告)号：US20110269826A1
公开(公告)日：2011-11-03
申请号：US13128813
申请日：2009-11-11
申请人： Susan M. Kingsman , Alan J. Kingsman , Scott Ralph , Kyriacos A. Mitrophanous , Stephane Palfi , Bechir Jarraya
发明人： Susan M. Kingsman , Alan J. Kingsman , Scott Ralph , Kyriacos A. Mitrophanous , Stephane Palfi , Bechir Jarraya
CPC分类号： A61K48/005 , C12N9/0073 , C12N9/78 , C12N9/88 , C12N2799/027 , C12N2799/04 , C12N2840/206 , C12N2840/50
摘要： The present invention provides methods for: (i) treating and/or preventing Parkinson's disease in a subject without causing cognitive impairment by using dopamine replacement gene therapy to maintain or restore constant physiological dopaminergic tone in both the dorsal and ventral striatum of the subject; (ii) normalising neuronal electrical activity in basal ganglia and/or subthalamic nucleus in a Parkinson's disease subject; and (iii) treating and/or preventing dyskinesias associated with oral L-dopa administration in a Parkinson's disease subject by administration of a vector system for dopamine replacement gene therapy to the subject.
摘要翻译： 本发明提供了以下方法：（i）通过使用多巴胺替代基因治疗在受试者的背侧和腹侧纹状体中维持或恢复不断的生理多巴胺能调节，治疗和/或预防受试者中的帕金森病，而不引起认知障碍; （ii）在帕金森病受试者的基底神经节和/或丘脑底核中归一化神经元电活动; 和（iii）通过向受试者施用用于多巴胺替代基因治疗的载体系统来治疗和/或预防与帕金森病患者口服左旋多巴给药相关的运动障碍。
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公开(公告)号：US20090191230A1
公开(公告)日：2009-07-30
申请号：US11456530
申请日：2006-07-10
CPC分类号： A61K39/0011 , A61K31/675 , A61K38/44 , A61K45/06 , A61K2039/53 , C12Y114/13 , C12Y114/14001 , A61K2300/00
摘要： The invention provides the use of an enzyme and a prodrug in the manufacture of a medicament for use in inducing an anti-tumour immune response in a human patient.
摘要翻译： 本发明提供了酶和前药在制备用于在人类患者中诱导抗肿瘤免疫应答的药物中的用途。
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公开(公告)号：US07276488B2
公开(公告)日：2007-10-02
申请号：US10060585
申请日：2002-01-29
申请人： Alan J. Kingsman , Christopher R. Bebbington , Miles W. Carroll , Fiona M. Ellard , Susan M. Kingsman , Kevin A. Myers
发明人： Alan J. Kingsman , Christopher R. Bebbington , Miles W. Carroll , Fiona M. Ellard , Susan M. Kingsman , Kevin A. Myers
IPC分类号： A61K48/00
CPC分类号： C07K14/485 , A61K2039/505 , C07K14/70532 , C07K16/28 , C07K16/30 , C07K2317/622 , C07K2319/00 , C07K2319/02 , C12N15/87 , C12N2799/027
摘要： The present invention provides a vector system comprising a nucleotide sequence coding for an antibody. In particular, the present invention relates to the use of such a vector system in a subject, where the nucleotide sequence is expressed in vivo to produce said antibody.
摘要翻译： 本发明提供了包含编码抗体的核苷酸序列的载体系统。 特别地，本发明涉及这样的载体系统在受试者中的用途，其中核苷酸序列在体内表达以产生所述抗体。
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公开(公告)号：US07718627B2
公开(公告)日：2010-05-18
申请号：US11380188
申请日：2006-04-25
申请人： Susan M. Kingsman , Christopher R. Bebbington , Fiona M. Ellard , Miles W. Carroll , Kevin A. Myers
发明人： Susan M. Kingsman , Christopher R. Bebbington , Fiona M. Ellard , Miles W. Carroll , Kevin A. Myers
IPC分类号： A61K31/70
CPC分类号： C07K16/40 , C07K14/485 , C07K14/70532 , C07K16/30 , C07K2317/622 , C07K2319/00 , C07K2319/02 , C07K2319/30 , C12N15/87 , C12N2799/027
摘要： A vector comprising a nucleotide sequence of interest (“NOI”) encoding a product of interest (“POI”) is described. The NOI and/or the POI is capable of recognizing a tumor, such that in use the vector is capable of delivering the NOI and/or the POI to the tumor.
摘要翻译： 描述了包含编码感兴趣的产物（“POI”）的感兴趣的核苷酸序列（“NOI”）的载体。 NOI和/或POI能够识别肿瘤，使得在使用中载体能够将NOI和/或POI递送至肿瘤。
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