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    Aligned fiber diagnostic chromatography with positive and negative controls
    2.
    发明授权
    Aligned fiber diagnostic chromatography with positive and negative controls 失效
    具有阳性和阴性对照的对齐纤维诊断色谱

    公开(公告)号：US5876918A

    公开(公告)日：1999-03-02

    申请号：US513763

    申请日：1995-08-29

    申请人： Norman Wainwright ,  Steven H. Boyd

    发明人： Norman Wainwright ,  Steven H. Boyd

    IPC分类号： B01L3/02 ,  G01N30/32 ,  G01N30/52 ,  G01N30/60 ,  G01N30/88 ,  G01N35/10 ,  C12Q1/00 ,  G01N33/543

    CPC分类号： G01N30/6052 ,  B01L3/0275 ,  G01N2030/525 ,  G01N2030/8827 ,  G01N2035/1055 ,  G01N30/32 ,  G01N30/6069

    摘要： A preactivated chromatography tip having covalent coupling functionality within a hydrodynamically designed micropipette tip assembly. The preactivated micro-channeled element provides high fluid directionality and throughput with minimum backpressure. A fibrous capture element is designed to optimize surface area to volume ratio, chemical resistance, a specific directional flow pattern, low non-specific binding of ligands, small sample volume, speed and low background.

    摘要翻译： PCT No.PCT / US94 / 02411 Sec。 371日期1995年8月29日 102（e）日期1995年8月29日PCT 1994年3月7日PCT公布。 公开号WO95 / 04037 日期1995年2月9日在流体力学设计的微量移液器吸头组件内具有共价偶联功能的预活化色谱尖端。 预活化的微通道元件以最小的背压提供高流体方向性和产量。 纤维捕获元件被设计为优化表面积与体积比，耐化学性，特定的方向流动模式，配体的低非特异性结合，小样品体积，速度和低背景。

    System and apparatus for automatically transferring media
    4.
    发明授权
    System and apparatus for automatically transferring media 失效
    用于自动传送媒体的系统和设备

    公开(公告)号：US5759847A

    公开(公告)日：1998-06-02

    申请号：US503081

    申请日：1995-07-14

    申请人： Ruth F. Eden ,  Steven H. Boyd

    发明人： Ruth F. Eden ,  Steven H. Boyd

    IPC分类号： C12M1/26 ,  C12M1/36

    CPC分类号： C12M27/16 ,  C12M41/36

    摘要： A system (100), methods and apparatuses for transferring a primary medium (7) after incubating of the medium (7) are described. The system (100) includes a container (1) for containing a growth medium (7) and a sample (8) to be tested for the presence of target microorganisms. A system (100) for transferring a primary medium (7) after incubating of the medium (7) is also described. The system (100) includes an automated pouring mechanism for automatically pouring the primary medium (7) into a plurality of media receiving vessels (11) and a metering mechanism for metering the amount of medium (7) poured into each media receiving vessel (11). An automated tilting apparatus (20) tilts the container (1) to actuate pouring of a predetermined amount of the medium (7) from the container (1) into the media receiver (11). An apparatus (20) for automatically transferring medium (7) for collection and testing of microorganisms is also described. It includes an automated tilting apparatus (20) for automatically tilting the container (1) to actuate pouring of a predetermined amount of medium (7) from the container (1) into a media receiver (11). A container (1) for transferring medium (7) for collection and testing of microorganisms is also described. A manifold (12) for controlling the flow of a primary medium (7) transferred from a from a container (1) to a receptacle vessel (11) is also described.

    摘要翻译： 描述了一种系统（100），用于在培养基（7）之后转移一次介质（7）的方法和装置。 系统（100）包括用于容纳生长培养基（7）的容器（1）和待测微生物存在的样品（8）。 还描述了用于在培养基（7）后转移第一介质（7）的系统（100）。 系统（100）包括自动倾倒机构，用于将主介质（7）自动地浇注到多个介质接收容器（11）中;以及计量机构，用于计量倾倒到每个介质接收容器（11）中的介质 ）。 自动倾斜装置（20）使容器（1）倾斜以致将预定量的介质（7）从容器（1）倾倒到介质接收器（11）中。 还描述了用于自动转移用于收集和测试微生物的介质（7）的装置（20）。 它包括一个自动倾斜装置（20），用于使容器（1）自动倾斜以致使预定量的介质（7）从容器（1）倾倒到介质接收器（11）中。 还描述了用于传送用于收集和测试微生物的介质（7）的容器（1）。 还描述了用于控制从容器（1）转移到容器容器（11）的主介质（7）的流动的歧管（12）。

    Multiple chromogen enzyme targeting (MCET) for use in bacterial contamination monitoring
    5.
    发明授权
    Multiple chromogen enzyme targeting (MCET) for use in bacterial contamination monitoring 失效
    用于细菌污染监测的多种色素酶靶向（MCET）

    公开(公告)号：US5510243A

    公开(公告)日：1996-04-23

    申请号：US264130

    申请日：1994-06-21

    申请人： Steven H. Boyd ,  Norman R. Wainwright

    发明人： Steven H. Boyd ,  Norman R. Wainwright

    IPC分类号： C12Q1/10 ,  C12Q1/54 ,  C12Q1/34 ,  C12N1/00 ,  C12P39/00 ,  C12Q1/04

    CPC分类号： C12Q1/54 ,  C12Q1/10 ,  G01N2333/245 ,  Y10S435/849

    摘要： The invention is directed to a process and a medium for simultaneous determination of the number and presence of living fecal coliform and Escherichia coli in a sample comprising 6-O-alpha-D-galactopyranosyl-D-glucose or isopropyl-beta-D-thiogalactoside as a galactosidase inducer and methyl-beta-D-glucuronide as a glucuronidase inducer. The sterile semi-solid medium also comprises non-target bacterial inhibitors, target bacterial enhancers, and multiple fluorogen and/or chromogan substrates that produce color and fluorescence upon cleavage by a specific enzyme expressed by the target bacteria in which expression is enhanced. The simultaneous detection of total coliforms via its expression of beta-galactosidase, and Escherichia coli as the target bacteria via its expression of beta-galactosidase and beta-glucuronidase is achieved rapidly and efficiently using this medium.

    摘要翻译： 本发明涉及用于同时测定包含6-O-α-D-吡喃半乳糖基-D-葡萄糖或异丙基-β-D-硫代半乳糖苷的样品中活的大肠杆菌和大肠杆菌数量和存在的方法和介质 作为半乳糖苷酶诱导剂和甲基-β-D-葡糖苷酸作为葡糖醛酸糖苷酶诱导剂。 无菌半固体培养基还包含非目标细菌抑制剂，靶细菌增生剂，以及由表达增强的靶细菌表达的特异性酶切割时产生颜色和荧光的多种氟和/或色原体底物。 通过其β-半乳糖苷酶的表达和大肠杆菌通过其β-半乳糖苷酶和β-葡糖苷酸酶的表达同时检测总大肠杆菌作为靶细菌，使用该培养基快速有效地实现。

    Activated immunodiagnostic pipette tips
    6.
    发明授权
    Activated immunodiagnostic pipette tips 失效
    活化的免疫诊断移液器吸头

    公开(公告)号：US5171537A

    公开(公告)日：1992-12-15

    申请号：US695955

    申请日：1991-05-06

    申请人： Norman B. Wainwright ,  Steven H. Boyd

    发明人： Norman B. Wainwright ,  Steven H. Boyd

    IPC分类号： B01L3/02 ,  G01N33/543 ,  G01N35/10

    CPC分类号： G01N33/54366 ,  B01L3/0275 ,  B01L2300/0627 ,  G01N2035/1055

    摘要： The tip has intermediate its end at least two axially spaced projections which are formed on the wall of its axial bore to extend radially and part way into the bore to define therebetween a reaction chamber in the bore of the tip. A spherical receptor element is mounted in the reaction chamber for limited pulsating movement therein in response to the movement of a fluid sample into and out of the tip. Additional projections may be formed in the bore to increase the turbulence created by the sample as it moves through the tip. The spherical element is coated with a ligand having a specific affinity for a target molecule in the sample.

    摘要翻译： 尖端在其中间具有至少两个轴向间隔开的突起，其形成在其轴向孔的壁上，以径向和部分方式延伸到孔中，以在其中限定尖端孔中的反应室。 响应于流体样品进入和离开尖端的运动，球形接收器元件安装在反应室中用于有限的脉动运动。 在孔中可以形成另外的突起，以增加当样品移动通过尖端时由样品产生的湍流。 球形元件涂覆有对样品中靶分子具有特异性亲和性的配体。

    Process and apparatus for microbial filtration and bacterial injection for one or more environmental contaminants
    7.
    发明授权
    Process and apparatus for microbial filtration and bacterial injection for one or more environmental contaminants 失效
    用于一种或多种环境污染物的微生物过滤和细菌注射的方法和装置

    公开(公告)号：US07166211B1

    公开(公告)日：2007-01-23

    申请号：US10833409

    申请日：2004-04-28

    申请人： Steven H. Boyd

    发明人： Steven H. Boyd

    IPC分类号： C02F3/00

    CPC分类号： A01K63/04 ,  C02F3/006 ,  C02F3/06 ,  C02F3/10 ,  C02F3/102 ,  C02F3/286 ,  C02F3/288 ,  C02F3/348 ,  C02F2103/20 ,  C02F2209/22 ,  Y02W10/15

    摘要： Scaleable bacterial injection, aeration and filtration system to effectively control microbial community function of aquatic systems. The present invention is a substantially simplified system with respect to handling growth and dispersion of microorganisms in solution and is also adaptable to many different end-use applications, including treatment of ornamental fish and aquaculture, pond and streams and sewage treatment systems. Initial microorganism materials are provided in the form of aqueous suspensions, which are incorporated into a large volume of water in a vessel. Microorganisms are entrapped inside a carrier that serves as a physical enclosure for cell retention. It has a porous structure to facilitate the diffusion of substances, such as ammonia, nitrate, nutrients, fuels and organic carbon, into its internal void volume, where substrate reduction is accomplished by entrapped cells. Specific species of microbes are injected into a filtration matrix that is bathed in oxygenated effluent and a portion of which is dispensed into an aqueous environment. The apparatus can be electronic, air or fluid driven and injected with the desired microbial species, preferably multiple time per day. Multi-stage media can be placed into packed filter units of convenient sizes for wastewater regeneration as well as natural water pollution reduction. The reactor canisters containing entrapped cells are portable and can be assembled off-site and then delivered to the field for installation and immediate operation. Bio-solid reduction, odor reduction, unplugging of leaching fields, conversion and reduction of nitrogen species in natural waters such as lakes and ponds, etc. can be accomplished.

    摘要翻译： 可扩展的细菌注射，曝气和过滤系统，有效控制水生系统的微生物群落功能。 本发明是关于处理微生物在溶液中的生长和分散的基本简化的系统，并且也适用于许多不同的最终用途应用，包括观赏鱼和水产养殖，池塘和溪流以及污水处理系统的处理。 初始微生物材料以水性悬浮液的形式提供，其被并入容器中的大量水中。 微生物被捕获在用作细胞保留的物理外壳的载体内。 它具有多孔结构，以促进物质如氨，硝酸盐，营养物质，燃料和有机碳的扩散到其内部空隙体积中，其中底物还原通过包埋的细胞完成。 将特定种类的微生物注入过滤基质中，沐浴在含氧流出物中，其一部分被分配到含水环境中。 该设备可以是电子的，空气的或流体驱动的并且注入所需的微生物物种，优选每天多次。 多级介质可以放置在方便尺寸的包装过滤器中，用于废水再生以及天然水污染减少。 含有捕获的细胞的反应器罐是便携式的，可以非现场组装，然后输送到现场进行安装和立即操作。 可以实现生物固体减少，气味降低，浸出场的拔出，天然水如湖泊和池塘等中的氮物质的转化和还原。