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    Recombinant albumins fused with poly-cysteine peptide and the methods for preparing the same
    2.
    发明授权
    Recombinant albumins fused with poly-cysteine peptide and the methods for preparing the same 有权
    与多聚半胱氨酸肽融合的重组白蛋白及其制备方法

    公开(公告)号：US08541378B2

    公开(公告)日：2013-09-24

    申请号：US12792186

    申请日：2010-06-02

    申请人： Hyung Jun Ahn ,  Ick Chan Kwon ,  Kuiwon Choi ,  Kwangmyeng Kim ,  Inchan Youn ,  Sehoon Kim

    发明人： Hyung Jun Ahn ,  Ick Chan Kwon ,  Kuiwon Choi ,  Kwangmyeng Kim ,  Inchan Youn ,  Sehoon Kim

    IPC分类号： A61K38/10

    CPC分类号： C07K14/76 ,  A61K47/643 ,  C07K14/765 ,  C07K2319/20

    摘要： The present invention relates to recombinant albumins fused with poly-cysteine peptide and methods for preparing the same, more precisely recombinant albumins in which cysteines that can be used for drug binding are amplified at N-terminal and C-terminal of the albumin and methods for preparing the same. The recombinant albumin of the present invention demonstrates improved albumin-drug conjugation efficiency when it is used for drug delivery system, indicating that it can effectively deliver a large amount of drug to a target tissue. At the same time, the recombinant albumin of the present invention can be used as an excellent drug deliverer with reduced side effects, compared with the conventional albumin carriers, by regulating the amount of drug conjugated to each unit of albumin by regulating the number of cysteine fused thereto. In addition, the recombinant albumin of the present invention can be used for the screening of a novel drug and for the non-invasive real-time diagnosis and treatment of disease by combining with a fluorescent material or a contrast agent for molecular imaging.

    摘要翻译： 本发明涉及与多聚半胱氨酸肽融合的重组白蛋白及其制备方法，更准确地说，重组白蛋白，其中可用于药物结合的半胱氨酸在白蛋白的N末端和C末端扩增， 准备一样 本发明的重组白蛋白在用于药物递送系统时表现出改善的白蛋白 - 药物共轭效率，表明其可有效地将大量药物递送至靶组织。 同时，与常规白蛋白载体相比，本发明的重组白蛋白可以用作优异的药物递送剂，通过调节半胱氨酸数目调节与每单位白蛋白结合的药物量 融合。 此外，本发明的重组白蛋白可以用于筛选新药和用于分子成像的荧光材料或造影剂的非侵入性实时诊断和治疗疾病。

    Nanoparticles of light emissive polymers and preparation method thereof
    3.
    发明授权
    Nanoparticles of light emissive polymers and preparation method thereof 有权
    光发射聚合物纳米颗粒及其制备方法

    公开(公告)号：US08367042B2

    公开(公告)日：2013-02-05

    申请号：US12576193

    申请日：2009-10-08

    申请人： Sehoon Kim ,  Ick Chan Kwon ,  Kwangmeyung Kim ,  Kuiwon Choi ,  Chang-Keun Lim

    发明人： Sehoon Kim ,  Ick Chan Kwon ,  Kwangmeyung Kim ,  Kuiwon Choi ,  Chang-Keun Lim

    IPC分类号： A61B5/00

    CPC分类号： A61K49/0019 ,  A61K49/0093

    摘要： Disclosed are nanoparticles of a light emissive polymer, comprising nanoparticles of a cyano-substituted poly(arylene vinylene) polymer; and a biocompatible surfactant adsorbed to the surface of the nanoparticles of the polymer, and preparation method thereof, wherein the method comprises: (1) uniformly mixing a dialdehyde monomer represented by a general formula OHC—Ar1—CHO, a dicyanide monomer represented by a general formula NC—Ar2—CN, and a liquid surfactant; (2) adding water to the resulting mixture to prepare an aqueous micelle dispersion; and (3) adding a polymerization catalyst to the aqueous micelle dispersion, followed by carrying out colloidal polymerization of the resulting mixture at room temperature under an atmosphere. The nanoparticles of the light emissive polymer of the invention are stabilized with a biocompatible surfactant, so that it can form a stable aqueous dispersion phase, and has particle size and fluorescence efficiency suitable for a biomolecular marker or a cell or in vivo imaging; therefore, it can be used as a cell or in vivo light emission contrast agent.

    摘要翻译： 公开了包含氰基取代的聚（亚芳基亚乙烯基）聚合物的纳米颗粒的发光聚合物的纳米颗粒; 和吸附在聚合物纳米颗粒表面的生物相容性表面活性剂及其制备方法，其中所述方法包括：（1）均匀混合由通式OHC-Ar1-CHO表示的二醛单体，由 通式NC-Ar2-CN和液体表面活性剂; （2）向所得混合物中加入水以制备水性胶束分散体; 和（3）将聚合催化剂加入到水性胶束分散体中，然后在室温下在气氛下进行所得混合物的胶体聚合。 本发明的光发射聚合物的纳米颗粒用生物相容的表面活性剂稳定，从而可以形成稳定的水分散相，并具有适用于生物分子标记或细胞或体内成像的粒度和荧光效率; 因此，它可以用作细胞或体内发光造影剂。

    NANOPARTICLES OF LIGHT EMISSIVE POLYMERS AND PREPARATION METHOD THEREOF
    4.
    发明申请
    NANOPARTICLES OF LIGHT EMISSIVE POLYMERS AND PREPARATION METHOD THEREOF 有权
    光散射聚合物纳米微粒及其制备方法

    公开(公告)号：US20100290999A1

    公开(公告)日：2010-11-18

    申请号：US12576193

    申请日：2009-10-08

    申请人： Sehoon KIM ,  Ick Chan KWON ,  Kwangmeyung KIM ,  Kuiwon CHOI ,  Chang-Keun LIM

    发明人： Sehoon KIM ,  Ick Chan KWON ,  Kwangmeyung KIM ,  Kuiwon CHOI ,  Chang-Keun LIM

    IPC分类号： A61K49/00 ,  C08G14/02 ,  C12Q1/02

    CPC分类号： A61K49/0019 ,  A61K49/0093

    摘要： Disclosed are nanoparticles of a light emissive polymer, comprising nanoparticles of a cyano-substituted poly(arylene vinylene) polymer; and a biocompatible surfactant adsorbed to the surface of the nanoparticles of the polymer, and preparation method thereof, wherein the method comprises: (1) uniformly mixing a dialdehyde monomer represented by a general formula OHC—Ar1—CHO, a dicyanide monomer represented by a general formula NC—Ar2—CN, and a liquid surfactant; (2) adding water to the resulting mixture to prepare an aqueous micelle dispersion; and (3) adding a polymerization catalyst to the aqueous micelle dispersion, followed by carrying out colloidal polymerization of the resulting mixture at room temperature under an atmosphere. The nanoparticles of the light emissive polymer of the invention are stabilized with a biocompatible surfactant, so that it can form a stable aqueous dispersion phase, and has particle size and fluorescence efficiency suitable for a biomolecular marker or a cell or in vivo imaging; therefore, it can be used as a cell or in vivo light emission contrast agent.

    摘要翻译： 公开了包含氰基取代的聚（亚芳基亚乙烯基）聚合物的纳米颗粒的发光聚合物的纳米颗粒; 和吸附在聚合物纳米颗粒表面的生物相容性表面活性剂及其制备方法，其中所述方法包括：（1）均匀混合由通式OHC-Ar1-CHO表示的二醛单体，由 通式NC-Ar2-CN，和液体表面活性剂; （2）向所得混合物中加入水以制备水性胶束分散体; 和（3）将聚合催化剂加入到水性胶束分散体中，然后在室温下在气氛下进行所得混合物的胶体聚合。 本发明的光发射聚合物的纳米颗粒用生物相容的表面活性剂稳定，从而可以形成稳定的水分散相，并具有适用于生物分子标记或细胞或体内成像的粒度和荧光效率; 因此，它可以用作细胞或体内发光造影剂。

    RECOMBINANT ALBUMINS FUSED WITH POLY-CYSTEINE PEPTIDE AND THE METHODS FOR PREPARING THE SAME
    5.
    发明申请
    RECOMBINANT ALBUMINS FUSED WITH POLY-CYSTEINE PEPTIDE AND THE METHODS FOR PREPARING THE SAME 有权
    与聚胞苷肽融合的重组脂质体及其制备方法

    公开(公告)号：US20100310468A1

    公开(公告)日：2010-12-09

    申请号：US12792186

    申请日：2010-06-02

    申请人： Hyung Jun AHN ,  Ick Chan KWON ,  Kuiwon CHOI ,  Kwangmyeng KIM ,  Inchan YOUN ,  Sehoon KIM

    发明人： Hyung Jun AHN ,  Ick Chan KWON ,  Kuiwon CHOI ,  Kwangmyeng KIM ,  Inchan YOUN ,  Sehoon KIM

    IPC分类号： C07K14/76 ,  A61K47/48 ,  C12N15/81 ,  C12N1/19 ,  C12P21/02 ,  A61K49/00

    CPC分类号： C07K14/76 ,  A61K47/643 ,  C07K14/765 ,  C07K2319/20

    摘要： The present invention relates to recombinant albumins fused with poly-cysteine peptide and methods for preparing the same, more precisely recombinant albumins in which cysteines that can be used for drug binding are amplified at N-terminal and C-terminal of the albumin and methods for preparing the same. The recombinant albumin of the present invention demonstrates improved albumin-drug conjugation efficiency when it is used for drug delivery system, indicating that it can effectively deliver a large amount of drug to a target tissue. At the same time, the recombinant albumin of the present invention can be used as an excellent drug deliverer with reduced side effects, compared with the conventional albumin carriers, by regulating the amount of drug conjugated to each unit of albumin by regulating the number of cysteine fused thereto. In addition, the recombinant albumin of the present invention can be used for the screening of a novel drug and for the non-invasive real-time diagnosis and treatment of disease by combining with a fluorescent material or a contrast agent for molecular imaging.

    摘要翻译： 本发明涉及与多聚半胱氨酸肽融合的重组白蛋白及其制备方法，更准确地说，重组白蛋白，其中可用于药物结合的半胱氨酸在白蛋白的N末端和C末端扩增， 准备一样 本发明的重组白蛋白在用于药物递送系统时表现出改善的白蛋白 - 药物共轭效率，表明其可有效地将大量药物递送至靶组织。 同时，与常规白蛋白载体相比，本发明的重组白蛋白可以用作优异的药物递送剂，通过调节半胱氨酸数目调节与每单位白蛋白结合的药物的量 融合。 此外，本发明的重组白蛋白可以用于筛选新药和用于分子成像的荧光材料或造影剂的非侵入性实时诊断和治疗疾病。

    Nanoparticles for two-photon activated photodynamic therapy and imaging
    7.
    发明申请
    Nanoparticles for two-photon activated photodynamic therapy and imaging 审中-公开
    纳米粒子用于双光子激活光动力治疗和成像

    公开(公告)号：US20090035576A1

    公开(公告)日：2009-02-05

    申请号：US11900334

    申请日：2007-09-10

    申请人： Paras N. Prasad ,  Sehoon Kim ,  Tymish Y. Ohulchanskyy ,  Ravindra K. Pandey

    发明人： Paras N. Prasad ,  Sehoon Kim ,  Tymish Y. Ohulchanskyy ,  Ravindra K. Pandey

    IPC分类号： B32B5/16

    CPC分类号： A61K41/0071 ,  A61K41/008 ,  A61K49/0021 ,  A61K49/0093 ,  C01B33/141 ,  G01N33/582 ,  G01N33/585 ,  Y10T428/2982

    摘要： The present invention provides organically modified silica (ORMOSIL) nanoparticles into which have been incorporated two-photon absorption dye molecules. The two photon absorption dye displays a unique aggregation induced fluorescence enhancement behavior. As a result ORMOSIL nanoparticles with high amounts of the dye can be prepared. These particles can be used for imaging. In one embodiment, the nanoparticles can additionally have incorporated therein a photosensitizer. The photosensitzer can be activated by intraparticle fluorescence resonance energy transfer (FRET) from the dye aggregates resulting in enhanced fluorescence and singlet oxygen generation from photosensitizer under two-photon excitation conditions. Such nanoparticles can be used for photodynamic therapy applications.

    摘要翻译： 本发明提供了已经掺入双光子吸收染料分子的有机改性二氧化硅（ORMOSIL）纳米颗粒。 两个光子吸收染料显示出独特的聚集诱导的荧光增强行为。 因此，可以制备具有大量染料的ORMOSIL纳米颗粒。 这些颗粒可用于成像。 在一个实施方案中，纳米颗粒另外可以掺入光敏剂。 光敏剂可以通过来自染料聚集体的颗粒内荧光共振能量转移（FRET）来激活，从而在双光子激发条件下从光敏剂产生增强的荧光和单线态氧产生。 这种纳米颗粒可用于光动力疗法应用。

    Nanoparticles for two-photon activated photodynamic therapy and imaging
    8.
    发明申请
    Nanoparticles for two-photon activated photodynamic therapy and imaging 审中-公开
    纳米粒子用于双光子激活光动力治疗和成像

    公开(公告)号：US20080233051A1

    公开(公告)日：2008-09-25

    申请号：US11983845

    申请日：2007-11-13

    申请人： Paras N. Prasad ,  Sehoon Kim ,  Tymish Y. Ohulchanskyy ,  Ravindra K. Pandey

    发明人： Paras N. Prasad ,  Sehoon Kim ,  Tymish Y. Ohulchanskyy ,  Ravindra K. Pandey

    IPC分类号： A61K9/14 ,  A61K31/695 ,  A61K49/00

    CPC分类号： A61K31/695 ,  A61K41/0071 ,  A61K41/008 ,  A61K49/0021 ,  A61K49/0093

    摘要： The present invention provides organically modified silica (ORMOSIL) nanoparticles into which have been incorporated two-photon absorption dye molecules. The two photon absorption dye displays a unique aggregation induced fluorescence enhancement behavior. As a result ORMOSIL nanoparticles with high amounts of the dye can be prepared. These particles can be used for imaging. In one embodiment, the nanoparticles can additionally have incorporated therein a photosensitizer. The photosensitizer can be activated by intraparticle fluorescence resonance energy transfer (FRET) from the dye aggregates resulting in enhanced fluorescence and singlet oxygen generation from photosensitizer under two-photon excitation conditions. Such nanoparticles can be used for photodynamic therapy applications.

    摘要翻译： 本发明提供了已经掺入双光子吸收染料分子的有机改性二氧化硅（ORMOSIL）纳米颗粒。 两个光子吸收染料显示出独特的聚集诱导的荧光增强行为。 因此，可以制备具有大量染料的ORMOSIL纳米颗粒。 这些颗粒可用于成像。 在一个实施方案中，纳米颗粒另外可以掺入光敏剂。 可以通过来自染料聚集体的颗粒内荧光共振能量转移（FRET）来激活光敏剂，从而在双光子激发条件下从光敏剂产生增强的荧光和单线态氧产生。 这种纳米颗粒可用于光动力疗法应用。