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公开(公告)号：US12087568B2
公开(公告)日：2024-09-10
申请号：US18456678
申请日：2023-08-28
Applicant: Standard BioTools Canada Inc.
Inventor： Alexander Loboda , Raymond Jong , Michael Sullivan , Serguei Vorobiev , Robert Rotenberg , Emil D. Stratulativ , Maxim Voronov , Mark Armstrong
CPC classification number: H01J49/105 , H01J49/0027 , H01J49/0463 , H05H1/2406 , H05H1/30
Abstract: Inductively coupled plasma (ICP) analyzers use an ICP torch to generate a plasma in which a sample is atomized an ionized. Analysis of the atomic ions can be performed by atomic analysis, such as mass spectrometry (MS) or atomic emission spectrometry (AES). Particle based ICP analysis includes analysis of particles such as cells, beads, or laser ablation plumes, by atomizing and ionizing particles in an ICP torch followed by atomic analysis. In mass cytometry, mass tags of particles are analyzed by mass spectrometry, such as by ICP-MS. Systems and methods of the subject application include one or more of: a demountable ICP torch holder assembly, an external ignition device; an ICP load coil comprising an annular fin, particle suspension sample introduction fluidics, and ICP analyzers thereof.
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公开(公告)号：US20240371622A1
公开(公告)日：2024-11-07
申请号：US18610570
申请日：2024-03-20
Applicant: Standard BioTools Canada Inc. , University of Ottawa
Inventor： Paul Corkum , Alexander V. Loboda
IPC: H01J49/04 , G01N33/68 , G02B21/33 , H01J37/20 , H01J37/28 , H01J49/00 , H01J49/02 , H01J49/06 , H01J49/10 , H01J49/14 , H01J49/16 , H01J49/40
Abstract: The present invention relates to the high resolution imaging of samples using imaging mass spectrometry (IMS) and to the imaging of biological samples by imaging mass cytometry (IMC®) in which labelling atoms are detected by IMS. LA-ICP-MS (a form of IMS in which the sample is ablated by a laser, the ablated material is then ionised in an inductively coupled plasma before the ions are detected by mass spectrometry) has been used for analysis of various substances, such as mineral analysis of geological samples, analysis of archaeological samples, and imaging of biological substances. However, traditional LA-ICP-MS systems and methods may not provide high resolution. Described herein are methods and systems for high resolution IMS and IMC.
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公开(公告)号：US20240345072A1
公开(公告)日：2024-10-17
申请号：US18409399
申请日：2024-01-10
Applicant: Standard BioTools Canada Inc.
Inventor： Olga Ornatsky , Qing Chang , Bedilu Allo , Xudong Lou , Alexander Bouzekri
IPC: G01N33/532 , C07K1/13 , G01N33/58
CPC classification number: G01N33/532 , C07K1/13 , G01N33/583
Abstract: The present disclosure relates to reagents and their use for elemental imaging mass spectrometry of biological samples.
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公开(公告)号：US20230236105A1
公开(公告)日：2023-07-27
申请号：US17786163
申请日：2020-12-18
Applicant: Standard BioTools Canada Inc.
Inventor： Bedilu ALLO , Xudong LOU , Peng LIU , Daniel MAJONIS , Anastasia MAVROPOULOS , Ladan GHEIRATMAND
IPC: G01N15/14 , G01N33/483
CPC classification number: G01N15/1429 , G01N33/483 , G01N2015/1006
Abstract: Described herein are reagents and methods for improving signal in imaging mass cytometry. Aspects include mass tags with a large number of labeling atoms, chemical modifications to mass tags and additional reagents to reduce background and/or maintain target binding of mass tagged specific binding partners (SBPs), and schemes for associating a plurality of mass tags with a single SBP. As such, embodiments include any combination of one or more reagents and their use. The reagents, kits and methods herein may be used for mass cytometry, including imaging mass cytometry. In some aspects, reagents, kits or methods may be used for delivery of a large number of radioisotopes to a target analyte, for example for therapeutic use or radiometric detection. In certain aspects, only non-radioactive isotopes may be used for mass cytometry.
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公开(公告)号：US20250044571A1
公开(公告)日：2025-02-06
申请号：US18887633
申请日：2024-09-17
Applicant: Standard BioTools Canada Inc.
Inventor： Daaf Sandkuijl , Alexander V. Loboda , Adam Carew , Khashayar Askarpour
Abstract: We describe in this application systems and methods for autofocusing in imaging mass spectrometry. The present application describes improvements over current IMS and IMC apparatus and methods through an autofocus component including a plurality of apertures in the autofocus system, such as a plurality of apertures arranged in 2 dimensions. As a plurality of apertures is used, the autofocus system provides redundancy in the event that measurement of focus on the sample from the illuminating radiation passed through one or more of the apertures fails so as to reduce the number of unsuccessful autofocus attempts.
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Patent Agency Ranking
公开(公告)号：US20230215714A1
公开(公告)日：2023-07-06
申请号：US17785887
申请日：2020-12-21
Applicant: Standard BioTools Canada Inc.
Inventor： Adam Carew , Daaf Sandkuijl , Alexander Loboda , Matthew Weel , Dmitry Bandura
CPC classification number: H01J49/0004 , H01J49/40 , H01J49/0463
Abstract: Described herein are systems and methods for imaging mass spectrometry, including imaging mass cytometry. Aspects of the subject application include apparatus and methods for imaging mass spectrometry (IMS) that improve speed of sample acquisition, signal sensitivity, and/or signal stability. Systems and methods described herein may minimize the transfer time and/or may minimize the spread of plumes of sample material ablated from a sample to be transferred to the components of the imaging mass spectrometer or mass cytometer that ionize and analyze the sample material.
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公开(公告)号：US20240369567A1
公开(公告)日：2024-11-07
申请号：US18644592
申请日：2024-04-24
Applicant: Standard BioTools Canada Inc.
Inventor： Alexander V. Loboda , Olga Ornatsky , Vladimir Baranov , Nikita Zabinyakov , Anastasia Mavropoulos , Daniel Majonis , Bedilu Allo , Taunia Closson
Abstract: Embodiments of the present invention relate to reagents and their use for elemental imaging mass spectrometry of biological samples. Embodiments include a method for quantifying one or more analytes within a sample. The method may include providing the sample. One or more analytes may be immobilised to a mass cytometry sample carrier. The sample may have been labelled with one or more mass-tagged SBPs including one or more labelling atoms. The method may include performing mass cytometry on the sample to determine a level of the one or more labelling atoms, where the level of the one or more labelling atoms corresponds to the copy number of the one or more analytes to quantify the analytes.
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公开(公告)号：US20240369566A1
公开(公告)日：2024-11-07
申请号：US18752407
申请日：2024-06-24
Applicant: Standard BioTools Canada Inc.
Inventor： Alexander Loboda , Raymond Jong , Michael Sullivan , Serguei Vorobiev , Robert Rotenberg , Emil D. Stratulativ , Maxim Voronov , Mark Armstrong
Abstract: Inductively coupled plasma (ICP) analyzers use an ICP torch to generate a plasma in which a sample is atomized an ionized. Analysis of the atomic ions can be performed by atomic analysis, such as mass spectrometry (MS) or atomic emission spectrometry (AES). Particle based ICP analysis includes analysis of particles such as cells, beads, or laser ablation plumes, by atomizing and ionizing particles in an ICP torch followed by atomic analysis. In mass cytometry, mass tags of particles are analyzed by mass spectrometry, such as by ICP-MS. Systems and methods of the subject application include one or more of: a demountable ICP torch holder assembly, an external ignition device; an ICP load coil comprising an annular fin, particle suspension sample introduction fluidics, and ICP analyzers thereof.
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公开(公告)号：US20240361304A1
公开(公告)日：2024-10-31
申请号：US18657098
申请日：2024-05-07
Applicant: Standard BioTools Canada Inc.
Inventor： Vladimir Baranov , Alexander Loboda , Michael Sullivan
CPC classification number: G01N33/5306 , G01N33/58 , H01J49/105 , G01N2458/15 , G01N2560/00
Abstract: Analyzing samples injected into an inductively coupled plasma source can be improved by one or more of a stabilizing solution mixable with a sample prior to injection and a heated injector. The stabilizing solution can minimize the difference in osmotic pressure between the solution and the cells with a relatively low amount of dissolved solids (e.g., at or below about 0.2%). The stabilizing solution can contain a salt (e.g., ammonium nitrate) present in concentrations of at least 5 mM. The injector can be heated before and/or during injection. In some cases, heat from adjacent parts can be channeled into the injector to improve heating of the injector. An injector heated to sufficient temperatures can minimize solute buildup and can extend the usable time between cleanings. These improvements can be especially useful in elemental analysis, such as inductively coupled plasma mass spectrometry or inductively coupled plasma optical emission spectrometry.
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公开(公告)号：US20240327549A1
公开(公告)日：2024-10-03
申请号：US18575268
申请日：2022-07-08
Inventor： Mitchell A. Winnik , Edmond Chi Ngae Wong , Yefeng Zhang , Daniel Majonis , Peng Liu
Abstract: The present disclosure relates to compounds of Formula I, element tags comprising soft metals, and methods and kits therefore for performing mass cytometry.
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