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公开(公告)号：US20120135474A1
公开(公告)日：2012-05-31
申请号：US13266762
申请日：2010-04-27
CPC分类号： C12N9/2434 , C12N9/2445 , C12P19/14 , C12Y302/01021
摘要： The present disclosure provides methods for the conversion of hemicellulose into fermentable sugars using enzymes isolated from Prevotella bryantii. Hemicellulose-degrading enzymes include an endoxylanase, a β-xylosidase, a bifunctional β-xylosidase and β-glucosidase, a bifunctional arabinofuranosidase and β-xylosidase, a glucuronidase, and an acetyl xylan esterase. The enzymes can be used to release sugars present in hemicellulose for subsequent fermentation to produce value-added products such as ethanol.
摘要翻译： 本公开提供了使用从布氏血杆菌（Prevotella bryantii）分离的酶将半纤维素转化为可发酵糖的方法。 半纤维素降解酶包括内切木聚糖酶，β-木糖苷酶，双功能的β-木糖苷酶和β-葡糖苷酶，双功能的阿拉伯呋喃糖苷酶和β-木糖苷酶，葡糖苷酸酶和乙酰木聚糖酯酶。 酶可用于释放存在于半纤维素中的糖，用于随后的发酵以产生增值产物如乙醇。
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2.发明申请公开(公告)号：US20110237448A1
公开(公告)日：2011-09-29
申请号：US12885270
申请日：2010-09-17
CPC分类号： C12N9/18 , C07K2319/20 , C12Y301/01006
摘要： The present disclosure relates to isolated polynucleotides with two polynucleotide sequences linked within one open reading frame, in which the first polynucleotide sequence encodes a peptide that binds to a carbohydrate. The present disclosure also relates to vectors and genetically modified host cells containing such isolated polynucleotides and polypeptides encoded by such isolated polynucleotides. The present disclosure further relates to methods of increasing the ability of a recombinant protein to bind to a carbohydrate and methods of identifying a protein having an ability to bind to a carbohydrate.
摘要翻译： 本公开涉及具有在一个开放阅读框内连接的两个多核苷酸序列的分离的多核苷酸，其中第一多核苷酸序列编码与碳水化合物结合的肽。 本公开还涉及载体和遗传修饰的宿主细胞，其含有由分离的多核苷酸编码的分离的多核苷酸和多肽。 本公开还涉及增加重组蛋白质结合碳水化合物的能力的方法和鉴定具有结合碳水化合物的能力的蛋白质的方法。
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公开(公告)号：US09012186B2
公开(公告)日：2015-04-21
申请号：US13266762
申请日：2010-04-27
CPC分类号： C12N9/2434 , C12N9/2445 , C12P19/14 , C12Y302/01021
摘要： The present disclosure provides methods for the conversion of hemicellulose into fermentable sugars using enzymes isolated from Prevotella bryantii. Hemicellulose-degrading enzymes include an endoxylanase, a β-xylosidase, a bifunctional β-xylosidase and β-glucosidase, a bifunctional arabinofuranosidase and β-xylosidase, a glucuronidase, and an acetyl xylan esterase. The enzymes can be used to release sugars present in hemicellulose for subsequent fermentation to produce value-added products such as ethanol.
摘要翻译： 本公开提供了使用从布氏血杆菌（Prevotella bryantii）分离的酶将半纤维素转化为可发酵糖的方法。 半纤维素降解酶包括内切木聚糖酶，β-木糖苷酶，双功能的β-木糖苷酶和β-葡糖苷酶，双功能的阿拉伯呋喃糖苷酶和β-木糖苷酶，葡糖苷酸酶和乙酰木聚糖酯酶。 酶可用于释放存在于半纤维素中的糖，用于随后的发酵以产生增值产物如乙醇。
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公开(公告)号：US09765371B2
公开(公告)日：2017-09-19
申请号：US13997170
申请日：2011-12-20
申请人： Yejun Han , Xiaoyun Su , Dylan Dodd , Roderick I. Mackie , Isaac K. O. Cann
发明人： Yejun Han , Xiaoyun Su , Dylan Dodd , Roderick I. Mackie , Isaac K. O. Cann
IPC分类号： C12P19/14 , C12P7/04 , C12P7/16 , C12N9/18 , C12N9/26 , C12N9/42 , C12N1/20 , C12P21/06 , C12P19/34 , C12N9/24 , C12P7/14 , C12P19/02
CPC分类号： C12P19/14 , C12N9/18 , C12N9/2402 , C12N9/2405 , C12N9/2434 , C12N9/248 , C12N9/2482 , C12P7/04 , C12P7/14 , C12P7/16 , C12P19/02 , C12Y301/01072 , C12Y302/01008 , C12Y302/01055 , C12Y302/01072 , C12Y302/01139
摘要： The disclosure provides thermostable enzymes isolated from Caldicellulosiruptor bescii and fragments thereof useful for the degradation of cellulose and/or hemicellulose, including thermostable cellulases and hemicellulases. The disclosure further provides nucleic acids encoding the thermostable enzymes of the disclosure. The disclosure also provides methods for the conversion of cellulose and hemicellulose into fermentable sugars using thermostable enzymes of the disclosure. The disclosure also provides enzyme cocktails containing multiple enzymes disclosed herein. The enzymes can be used to release sugars present in cellulose or hemicellulose for subsequent fermentation to produce value-added products.
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5.发明授权公开(公告)号：US08357518B2
公开(公告)日：2013-01-22
申请号：US13114907
申请日：2011-05-24
申请人： Yejun Han , Dylan Dodd , Satish Nair , Roderick I. Mackie , Isaac K. O. Cann
发明人： Yejun Han , Dylan Dodd , Satish Nair , Roderick I. Mackie , Isaac K. O. Cann
CPC分类号： C12N9/2494 , C11D3/386 , C12P7/10 , C12Y204/01078 , D06L4/40 , D21C9/1026 , D21C9/1063 , D21H11/12 , Y02E50/16 , Y02E50/17
摘要： The present disclosure relates to hydrolysis of mannan-containing poly- or oligo-saccharides by use of a polypeptide having endo-β-mannanase activity. In particular the present disclosure relates to compositions comprising a bacterial endo-β-mannanase, polynucleotides encoding the endo-β-mannanase, and methods of use thereof.
摘要翻译： 本公开涉及通过使用具有内切 - 甘露聚糖酶活性的多肽来水解含甘露聚糖的多糖或寡糖。 特别地，本公开涉及包含细菌内切 - 甘露聚糖酶，编码内切 - 甘露聚糖酶的多核苷酸的组合物及其使用方法。
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6.发明申请公开(公告)号：US20110294166A1
公开(公告)日：2011-12-01
申请号：US13114907
申请日：2011-05-24
申请人： Yejun HAN , Dylan Dodd , Satish Nair , Roderick I. Mackie , Isaac K. O. Cann
发明人： Yejun HAN , Dylan Dodd , Satish Nair , Roderick I. Mackie , Isaac K. O. Cann
IPC分类号： C12P19/00 , C12N15/63 , C12N1/21 , D06M16/00 , C12N1/19 , C12N5/10 , C12N7/01 , C12P7/14 , C07H21/04 , C12N1/15
CPC分类号： C12N9/2494 , C11D3/386 , C12P7/10 , C12Y204/01078 , D06L4/40 , D21C9/1026 , D21C9/1063 , D21H11/12 , Y02E50/16 , Y02E50/17
摘要： The present disclosure relates to hydrolysis of mannan-containing poly- or oligo-saccharides by use of a polypeptide having endo-β-mannanase activity. In particular the present disclosure relates to compositions comprising a bacterial endo-β-mannanase, polynucleotides encoding the endo-β-mannanase, and methods of use thereof.
摘要翻译： 本公开涉及通过使用具有内切 - 甘露聚糖酶活性的多肽来水解含甘露聚糖的多糖或寡糖。 特别地，本公开涉及包含细菌内切 - 甘露聚糖酶，编码内切 - 甘露聚糖酶的多核苷酸的组合物及其使用方法。
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