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    Methods for detection of botulinum neurotoxin
    1.
    发明授权
    Methods for detection of botulinum neurotoxin 有权
    肉毒杆菌神经毒素检测方法

    公开(公告)号：US08067192B2

    公开(公告)日：2011-11-29

    申请号：US12134092

    申请日：2008-06-05

    Applicant: Markus Kalkum ,  Karine Bagramyan

    Inventor： Markus Kalkum ,  Karine Bagramyan

    IPC: G01N33/573

    CPC classification number: G01N33/573 ,  C07K7/08 ,  C07K14/705 ,  G01N33/5308 ,  G01N33/542 ,  G01N33/569 ,  G01N2333/32 ,  G01N2333/33 ,  G01N2333/38

    Abstract: Provided herein is a large immuno-sorbent surface area assay (ALISSA) for rapid and sensitive detection of toxin or enzyme activity. This assay is designed to capture a low number of toxin or enzyme molecules and to measure their intrinsic protease activity via conversion of a fluorogenic or luminescent substrate. The ALISSA is significantly faster and more sensitive than methods routinely utilized in the art. This assay is applicable for use for detection of a variety of toxins or enzymes having proteolytic activity, such as botulinum neurotoxin, bacillus anthracis lethal factor, human chitinases, and aspergillus fumigatus proteases. Also provided are methods for constructing and identifying novel luminescent or fluorescent substrates suitable for use with the ALISSA method.

    Abstract translation: 本文提供了用于快速和灵敏检测毒素或酶活性的大型免疫吸附剂表面积测定（ALISSA）。 该测定被设计为捕获低数量的毒素或酶分子，并通过荧光或发光底物的转化来测量它们的内在蛋白酶活性。 ALISSA比本领域常规使用的方法显着更快和更灵敏。 该测定适用于检测各种具有蛋白水解活性的毒素或酶，如肉毒杆菌神经毒素，炭疽杆菌致死因子，人几丁质酶和烟曲霉蛋白酶。 还提供了用于构建和鉴定适用于ALISSA方法的新型发光或荧光底物的方法。

    Process and device for detecting microparticle movement
    2.
    发明授权
    Process and device for detecting microparticle movement 失效
    用于检测微粒运动的方法和装置

    公开(公告)号：US06467328B1

    公开(公告)日：2002-10-22

    申请号：US09555068

    申请日：2000-07-24

    Applicant: Markus Kalkum ,  Markus Kietzmann ,  Martin Müller

    Inventor： Markus Kalkum ,  Markus Kietzmann ,  Martin Müller

    IPC: G01N100

    CPC classification number: B01L3/0241 ,  G01N2035/00237 ,  G01N2035/1034

    Abstract: For the detection of the position and/or velocity of microparticles which move from dispensers of a dispensing head (30) to a target, a detector target with two linear bound, electro-acoustic or electro-optic interactive areas which fix a target plane is used. When microparticles impact on the interactive areas, detector signals are determined from which along with the dispensing head position motion parameters of the microparticles are obtained.

    Abstract translation: 为了检测从分配头（30）的分配器移动到目标的微粒的位置和/或速度，具有固定目标平面的两个线性束缚，电声或电光交互区域的检测器目标是 用过的。 当微粒冲击交互区域时，确定检测器信号与其一起获得微粒的分配头位置运动参数。

    Methods for detection of botulinum neurotoxin
    6.
    发明授权
    Methods for detection of botulinum neurotoxin 有权
    肉毒杆菌神经毒素检测方法

    公开(公告)号：US08753831B2

    公开(公告)日：2014-06-17

    申请号：US13600186

    申请日：2012-08-30

    Applicant: Markus Kalkum ,  Karine Bagramyan

    Inventor： Markus Kalkum ,  Karine Bagramyan

    IPC: G01N21/76 ,  G01N21/64 ,  G01N33/53

    CPC classification number: G01N33/5308 ,  C07K7/08 ,  C07K14/705 ,  G01N33/5306 ,  G01N33/542 ,  G01N33/569 ,  G01N33/573 ,  G01N2333/32 ,  G01N2333/33 ,  G01N2333/38 ,  G01N2333/90241

    Abstract: Provided herein is a large immuno-sorbent surface area assay (ALISSA) for the rapid and sensitive detection of botulinum neurotoxins (BoNTs) and anthrax toxin. This assay is designed to capture a low number of toxin molecules and to measure their intrinsic protease activity via conversion of a fluorogenic or luminescent substrate. Also provided herein are novel peptides that can be specifically cleaved by BoNT and novel peptides that are resistant to cleavage by BoNT. The combination of these cleavable and control peptides can be used for implementation of an exemplary ALISSA used to specifically detect BoNT enzymatic activity. Furthermore, the ALISSA as described herein may also be used in a column based format for use in a high-throughput system for testing large quantities of samples.

    Abstract translation: 本文提供了用于快速和灵敏检测肉毒杆菌神经毒素（BoNT）和炭疽毒素的大型免疫吸附剂表面积测定（ALISSA）。 该测定设计用于捕获少量的毒素分子并通过转化荧光底物或发光底物测量其内在的蛋白酶活性。 本文还提供了可以被BoNT特异性切割的新型肽和对BoNT切割具有抗性的新型肽。 这些可切割和对照肽的组合可用于实施用于特异性检测BoNT酶活性的示例性ALISSA。 此外，如本文所述的ALISSA也可以以基于列的格式使用，以用于高通量系统中用于测试大量样品。

    METHODS FOR DETECTION OF BOTULINUM NEUROTOXIN
    9.
    发明申请
    METHODS FOR DETECTION OF BOTULINUM NEUROTOXIN 有权
    检测嗜碱性粒细胞毒素的方法

    公开(公告)号：US20090176259A1

    公开(公告)日：2009-07-09

    申请号：US12134092

    申请日：2008-06-05

    Applicant: Markus Kalkum ,  Karine Bagramyan

    Inventor： Markus Kalkum ,  Karine Bagramyan

    IPC: G01N33/573 ,  G01N33/569

    CPC classification number: G01N33/573 ,  C07K7/08 ,  C07K14/705 ,  G01N33/5308 ,  G01N33/542 ,  G01N33/569 ,  G01N2333/32 ,  G01N2333/33 ,  G01N2333/38

    Abstract: Provided herein is a large immuno-sorbent surface area assay (ALISSA) for rapid and sensitive detection of toxin or enzyme activity. This assay is designed to capture a low number of toxin or enzyme molecules and to measure their intrinsic protease activity via conversion of a fluorigenic or luminescent substrate. The ALISSA is significantly faster and more sensitive than methods routinely utilized in the art. This assay is applicable for use for detection of a variety of toxins or enzymes having proteolytic activity, such as botulinum neurotoxin, bacillus anthracis lethal factor, human chitinases, and aspergillus fumigatus proteases. Also provided are methods for constructing and identifying novel luminescent or fluorescent substrates suitable for use with the ALISSA method.

    Abstract translation: 本文提供了用于快速和灵敏检测毒素或酶活性的大型免疫吸附剂表面积测定（ALISSA）。 该测定被设计为捕获少量的毒素或酶分子，并通过转化荧光或发光底物来测量它们的内在蛋白酶活性。 ALISSA比本领域常规使用的方法显着更快和更灵敏。 该测定适用于检测各种具有蛋白水解活性的毒素或酶，如肉毒杆菌神经毒素，炭疽杆菌致死因子，人几丁质酶和烟曲霉蛋白酶。 还提供了用于构建和鉴定适用于ALISSA方法的新型发光或荧光底物的方法。

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