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公开(公告)号：US07364855B2
公开(公告)日：2008-04-29
申请号：US11119985
申请日：2005-05-02
CPC分类号： C12Q1/68 , C12Q1/6827 , C12Q1/6862 , C12Q2537/164 , C12Q2521/501
摘要： Ligation-based methods and kits are disclosed for determining the degree of methylation of one or more target nucleotides. In certain embodiments, the methylation status of one or more target nucleotides is determined by generating misligation products. In certain embodiments, at least one target nucleotide is amplified prior to the ligation reaction. In certain embodiments, at least one ligation product, at least one ligation product surrogate, at least one misligation product, at least one misligation product surrogate, or combinations thereof are amplified. In certain embodiments, one or more ligation probes comprise at least one nucleotide analog, at least one Modification, at least one mismatched nucleotide, or combinations thereof.
摘要翻译： 公开了基于连接的方法和试剂盒以确定一个或多个靶核苷酸的甲基化程度。 在某些实施方案中，通过产生错误产物来确定一个或多个靶核苷酸的甲基化状态。 在某些实施方案中，在连接反应之前扩增至少一种靶核苷酸。 在某些实施方案中，扩增至少一种连接产物，至少一种连接产物替代物，至少一种错误产物，至少一种错误产物替代物或其组合。 在某些实施方案中，一个或多个连接探针包含至少一个核苷酸类似物，至少一个修饰，至少一个错配核苷酸或其组合。
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公开(公告)号：US20110143343A1
公开(公告)日：2011-06-16
申请号：US12843877
申请日：2010-07-26
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68 , C12Q1/6827 , C12Q1/6862 , C12Q2537/164 , C12Q2521/501
摘要： Ligation-based methods and kits are disclosed for determining the degree of methylation of one or more target nucleotides. In certain embodiments, the methylation status of one or more target nucleotides is determined by generating misligation products. In certain embodiments, at least one target nucleotide is amplified prior to the ligation reaction. In certain embodiments, at least one ligation product, at least one ligation product surrogate, at least one misligation product, at least one misligation product surrogate, or combinations thereof are amplified. In certain embodiments, one or more ligation probes comprise at least one nucleotide analog, at least one Modification, at least one mismatched nucleotide, or combinations thereof.
摘要翻译： 公开了基于连接的方法和试剂盒以确定一个或多个靶核苷酸的甲基化程度。 在某些实施方案中，通过产生错误产物来确定一个或多个靶核苷酸的甲基化状态。 在某些实施方案中，在连接反应之前扩增至少一种靶核苷酸。 在某些实施方案中，扩增至少一种连接产物，至少一种连接产物替代物，至少一种错误产物，至少一种错误产物替代物或其组合。 在某些实施方案中，一个或多个连接探针包含至少一个核苷酸类似物，至少一个修饰，至少一个错配核苷酸或其组合。
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公开(公告)号：US07601821B2
公开(公告)日：2009-10-13
申请号：US11090830
申请日：2005-03-24
申请人： Mark R. Andersen , Kenneth J. Livak , Adam Broomer , Caifu Chen
发明人： Mark R. Andersen , Kenneth J. Livak , Adam Broomer , Caifu Chen
CPC分类号： C12Q1/6869 , C12Q1/6851 , C12Q2525/191 , C12Q2525/185 , C12Q2521/501 , C12Q2525/155 , C12Q2521/313 , C12Q2537/143 , C12Q2525/161
摘要： The present invention is directed to methods, reagents, and kits for detecting the presence or absence of (or quantifying) target polynucleotide sequences and proteins in at least one sample using encoding and decoding reactions. When a particular target polynucleotide is present in a sample for example, a reaction product is formed in the encoding reaction that includes addressable primer portions. At least one labeling probe and at least one address primer can be employed in the decoding amplification reaction thereby providing a detectable signal value depending upon whether a sequence is present or absent. In some embodiments, the encoding comprises a ligation reaction with linker probes, and single nucleotide polymorphisms (SNPs) are analyzed.
摘要翻译： 本发明涉及用于使用编码和解码反应检测至少一个样品中靶多核苷酸序列和蛋白质的存在或不存在（或定量）的方法，试剂和试剂盒。 当特定靶多核苷酸例如存在于样品中时，在包含可寻址引物部分的编码反应中形成反应产物。 可以在解码扩增反应中使用至少一个标记探针和至少一个地址引物，从而根据序列是否存在提供可检测的信号值。 在一些实施方案中，编码包含与接头探针的连接反应，并分析单核苷酸多态性（SNP）。
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公开(公告)号：US08323897B2
公开(公告)日：2012-12-04
申请号：US10723520
申请日：2003-11-26
申请人： Mark R. Andersen , David W. Ruff
发明人： Mark R. Andersen , David W. Ruff
CPC分类号： C12Q1/686 , C12Q1/6806 , C12Q1/6827 , C12Q1/6851 , C12Q2531/113 , C12Q2537/143 , C12Q2561/113 , C12Q2521/107 , C12Q2525/301 , C12Q2561/101
摘要： The present invention provides methods, reagents and kits for carrying out a variety of assays suitable for analyzing polynucleotides or samples that include an amplification step performed in a multiplex fashion. Also provided are methods for analyzing and improving the efficiency of amplification and for carrying out gene expression analysis.
摘要翻译： 本发明提供用于进行适于分析多核苷酸或样品的多种测定法的方法，试剂和试剂盒，所述测定包括以多种方式进行的扩增步骤。 还提供了用于分析和提高扩增效率和进行基因表达分析的方法。
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公开(公告)号：US07153658B2
公开(公告)日：2006-12-26
申请号：US10665671
申请日：2003-09-19
申请人： Mark R. Andersen , Michael W. Hunkapiller , Kenneth J. Livak , Eugene G. Spier , H. Michael Wenz
发明人： Mark R. Andersen , Michael W. Hunkapiller , Kenneth J. Livak , Eugene G. Spier , H. Michael Wenz
CPC分类号： C12Q1/6827 , C12Q2561/101 , C12Q2531/137 , C12Q2525/161
摘要： The present invention relates to methods and kits for detecting the presence or absence of (or quantitating) target nucleic acid sequences using ligation and amplification. The invention also relates to methods, reagents, and kits that employ addressable portions and labeled probes.
摘要翻译： 本发明涉及使用连接和扩增检测靶核酸序列的存在或不存在（或定量）的方法和试剂盒。 本发明还涉及采用可寻址部分和标记探针的方法，试剂和试剂盒。
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公开(公告)号：US20090142761A1
公开(公告)日：2009-06-04
申请号：US12111008
申请日：2008-04-28
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68 , C12Q1/6827 , C12Q1/6862 , C12Q2537/164 , C12Q2521/501
摘要： Ligation-based methods and kits are disclosed for determining the degree of methylation of one or more target nucleotides. In certain embodiments, the methylation status of one or more target nucleotides is determined by generating misligation products. In certain embodiments, at least one target nucleotide is amplified prior to the ligation reaction. In certain embodiments, at least one ligation product, at least one ligation product surrogate, at least one misligation product, at least one misligation product surrogate, or combinations thereof are amplified. In certain embodiments, one or more ligation probes comprise at least one nucleotide analog, at least one Modification, at least one mismatched nucleotide, or combinations thereof.
摘要翻译： 公开了基于连接的方法和试剂盒以确定一个或多个靶核苷酸的甲基化程度。 在某些实施方案中，通过产生错误产物来确定一个或多个靶核苷酸的甲基化状态。 在某些实施方案中，在连接反应之前扩增至少一种靶核苷酸。 在某些实施方案中，扩增至少一种连接产物，至少一种连接产物替代物，至少一种错误产物，至少一种错误产物替代物或其组合。 在某些实施方案中，一个或多个连接探针包含至少一个核苷酸类似物，至少一个修饰，至少一个错配核苷酸或其组合。
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