公开(公告)号：US20140227723A1

公开(公告)日：2014-08-14

申请号：US14233553

申请日：2012-07-18

Applicant: Donald E. Ingber ,  Michael Super ,  Jeffrey Charles Way ,  Mark J. Cartwright ,  Julia B. Berthet ,  Dinah R. Super ,  Martin M. Rottman ,  Alexander Watters

Inventor： Donald E. Ingber ,  Michael Super ,  Jeffrey Charles Way ,  Mark J. Cartwright ,  Julia B. Berthet ,  Dinah R. Super ,  Martin M. Rottman ,  Alexander Watters

IPC: C07K16/12 ,  G01N33/569 ,  C07K14/42

CPC classification number: C07K16/12 ,  A61K47/68 ,  A61K47/6815 ,  C07K14/42 ,  C07K2319/33 ,  G01N33/54353 ,  G01N33/56911 ,  G01N33/56916 ,  G01N33/56938 ,  G01N33/56961 ,  G01N2333/42 ,  G01N2333/4724 ,  Y02A50/53 ,  Y02A50/58

Abstract: Described herein are engineered microbe-targeting or microbe-binding molecules, kits comprising the same and uses thereof. Some particular embodiments of the microbe-targeting or microbe-binding molecules comprise a carbohydrate recognition domain of mannose-binding lectin, or a fragment thereof, linked to a portion of a Fc region. In some embodiments, the microbe-targeting molecules or microbe-binding molecules can be conjugated to a substrate, e.g., a magnetic microbead, forming a microbe-targeting substrate (e.g., a microbe-targeting magnetic microbead). Such microbe-targeting molecules and/or substrates and the kits comprising the same can bind and/or capture of a microbe and/or microbial matter thereof, and can thus be used in various applications, e.g., diagnosis and/or treatment of an infection caused by microbes such as sepsis in a subject or any environmental surface. Microbe-targeting molecules and/or substrates can be regenerated after use by washing with a low pH buffer or buffer in which calcium is insoluble.

Abstract translation: 本文描述了工程化的微生物靶向或微生物结合分子，包含其的试剂盒及其用途。 微生物靶向或微生物结合分子的一些具体实施方案包含与Fc区的一部分连接的甘露糖结合凝集素或其片段的碳水化合物识别结构域。 在一些实施方案中，微生物靶向分子或微生物结合分子可以与基底（例如磁性微珠）缀合，形成微生物靶向底物（例如，微生物靶向磁性微珠）。 这样的微生物靶向分子和/或底物及其包含的试剂盒可以结合和/或捕获微生物和/或其微生物物质，因此可以用于各种应用中，例如诊断和/或治疗感染 由微生物引起，如受试者或任何环境表面的败血症。 微生物靶向分子和/或底物可以在使用后通过用不溶于钙的低pH缓冲液或缓冲液洗涤而再生。

公开(公告)号：US09593160B2

公开(公告)日：2017-03-14

申请号：US14233553

申请日：2012-07-18

Applicant: Donald E. Ingber ,  Michael Super ,  Jeffrey Charles Way ,  Mark J. Cartwright ,  Julia B. Berthet ,  Dinah R. Super ,  Martin M. Rottman ,  Alexander Watters

Inventor： Donald E. Ingber ,  Michael Super ,  Jeffrey Charles Way ,  Mark J. Cartwright ,  Julia B. Berthet ,  Dinah R. Super ,  Martin M. Rottman ,  Alexander Watters

IPC: C07K14/42 ,  C07K16/12 ,  A61K47/48 ,  G01N33/543 ,  G01N33/569

CPC classification number: C07K16/12 ,  A61K47/68 ,  A61K47/6815 ,  C07K14/42 ,  C07K2319/33 ,  G01N33/54353 ,  G01N33/56911 ,  G01N33/56916 ,  G01N33/56938 ,  G01N33/56961 ,  G01N2333/42 ,  G01N2333/4724 ,  Y02A50/53 ,  Y02A50/58

Abstract: Described herein are engineered microbe-targeting or microbe-binding molecules, kits comprising the same and uses thereof. Some particular embodiments of the microbe-targeting or microbe-binding molecules comprise a carbohydrate recognition domain of mannose-binding lectin, or a fragment thereof, linked to a portion of a Fc region. In some embodiments, the microbe-targeting molecules or microbe-binding molecules can be conjugated to a substrate, e.g., a magnetic microbead, forming a microbe-targeting substrate (e.g., a microbe-targeting magnetic microbead). Such microbe-targeting molecules and/or substrates and the kits comprising the same can bind and/or capture of a microbe and/or microbial matter thereof, and can thus be used in various applications, e.g., diagnosis and/or treatment of an infection caused by microbes such as sepsis in a subject or any environmental surface. Microbe-targeting molecules and/or substrates can be regenerated after use by washing with a low pH buffer or buffer in which calcium is insoluble.

Abstract translation: 本文描述了工程化的微生物靶向或微生物结合分子，包含其的试剂盒及其用途。 微生物靶向或微生物结合分子的一些具体实施方案包括与Fc区的一部分连接的甘露糖结合凝集素或其片段的碳水化合物识别结构域。 在一些实施方案中，微生物靶向分子或微生物结合分子可以与基底（例如磁性微珠）缀合，形成微生物靶向底物（例如，微生物靶向磁性微珠）。 这样的微生物靶向分子和/或底物及其包含的试剂盒可以结合和/或捕获微生物和/或其微生物物质，因此可用于各种应用中，例如诊断和/或治疗感染 由微生物引起，如受试者或任何环境表面的败血症。 微生物靶向分子和/或底物可以在使用后通过用不溶于钙的低pH缓冲液或缓冲液洗涤而再生。

公开(公告)号：US06667038B1

公开(公告)日：2003-12-23

申请号：US09553863

申请日：2000-04-20

Applicant: Sam T. Donta ,  Mark J. Cartwright

Inventor： Sam T. Donta ,  Mark J. Cartwright

IPC: A61K3902

CPC classification number: C07K14/20 ,  A61K39/00 ,  C07K2319/00 ,  Y02A50/401 ,  Y02A50/472

Abstract: The present invention provides compositions and methods related to Borrelia burgdorferi toxin and antitoxin preparations. In particular, the present invention provides methods and compositions for the diagnosis of Lyme disease, as well as for use in treating subjects infected with B. burgdorferi through passive immunization, and vaccine development.

Abstract translation: 本发明提供了与布氏疏螺旋体毒素和抗毒素制剂有关的组合物和方法。 特别地，本发明提供了用于诊断莱姆病的方法和组合物，以及用于通过被动免疫来治疗感染B型胆固醇的受试者和疫苗开发。